Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,449,100 T→C T24A (ACG→GCG)  rplP ← 50S ribosomal subunit protein L16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,449,1000TC99.2% 493.5 / NA 134T24A (ACG→GCG) rplP50S ribosomal subunit protein L16
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (59/74);  minor base A (1/0);  total (60/74)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACA  >  NC_000913/3448954‑3449245
                                                                                                                                                  |                                                                                                                                                 
  gTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTg                                                                                                                                             <  4:348736/151‑1 (MQ=255)
    ttGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTgc                                                                                                                                            <  1:201236/150‑1 (MQ=255)
     tGTCCGGGAACACACGGATCCAGGTCTTACATTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGATTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGACGCTAACATCCGCACTCTgcgc                                                                                                                                          >  1:1295292/1‑151 (MQ=255)
       tCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGcc                                                                                                                                         >  1:724338/1‑150 (MQ=255)
        ccGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAg                                                                                                                                       <  2:1044998/151‑1 (MQ=255)
         cGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCa                                                                                                                                        <  2:579473/149‑1 (MQ=255)
           ggAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGAcc                                                                                                                                    >  3:152879/1‑151 (MQ=255)
               cacacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCgg                                                                                                                                <  4:167081/151‑1 (MQ=255)
                 cacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGtt                                                                                                                              <  2:247844/151‑1 (MQ=255)
                 cacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGtt                                                                                                                              <  2:912152/151‑1 (MQ=255)
                 cacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGtt                                                                                                                              >  1:1097630/1‑151 (MQ=255)
                   cGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTAc                                                                                                                            <  2:222251/151‑1 (MQ=255)
                    ggATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACg                                                                                                                           >  1:530029/1‑151 (MQ=255)
                     gATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACgg                                                                                                                          <  1:330820/151‑1 (MQ=255)
                     gATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCACGGTTACgg                                                                                                                          <  1:1532057/151‑1 (MQ=255)
                      aTCCAGATCTTTCCTTGACGCTTAACAGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCATCAGCTTTCCGACCGAAGCTGCCGAAGCTAACATCCGCCCCCTGCGCCAGACCGCGGTTACGGc                                                                                                                         <  1:1363603/151‑1 (MQ=255)
                       tCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGcc                                                                                                                        >  2:899933/1‑151 (MQ=255)
                        ccAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGcc                                                                                                                        <  1:126230/150‑1 (MQ=255)
                        ccAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCt                                                                                                                       <  2:1076032/151‑1 (MQ=255)
                        ccAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCt                                                                                                                       >  2:1274644/1‑151 (MQ=255)
                          aGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCttt                                                                                                                     >  2:1320860/1‑151 (MQ=255)
                          aGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCttt                                                                                                                     >  4:299194/1‑151 (MQ=255)
                           gATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtg                                                                                                                    >  1:556673/1‑151 (MQ=255)
                             tCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtgtg                                                                                                                  >  1:1717213/1‑151 (MQ=255)
                              cTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGc                                                                                                                 >  2:273529/1‑151 (MQ=255)
                               ttACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGc                                                                                                                 >  1:1606578/1‑150 (MQ=255)
                                tACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTtgt                                                                                                                   <  2:376/147‑1 (MQ=255)
                                tACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGc                                                                                                                 <  1:820811/149‑1 (MQ=255)
                                tACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAt                                                                                                               >  4:121658/1‑151 (MQ=255)
                                  ccTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAttt                                                                                                             >  2:408650/1‑151 (MQ=255)
                                  ccTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtt                                                                                                              <  2:1616507/150‑1 (MQ=255)
                                    ttGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtttt                                                                                                            >  2:494006/1‑150 (MQ=255)
                                     tGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtttt                                                                                                            <  2:1566331/149‑1 (MQ=255)
                                     tGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTa                                                                                                           <  4:152858/150‑1 (MQ=255)
                                      gACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACg                                                                                                         >  1:1115859/1‑151 (MQ=255)
                                       aCGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACgg                                                                                                        <  4:296391/151‑1 (MQ=255)
                                         gCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGaa                                                                                                      <  3:562208/151‑1 (MQ=255)
                                             aaCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAAtttt                                                                                                  >  1:1186785/1‑151 (MQ=255)
                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTg                                                                                                 <  2:695041/151‑1 (MQ=255)
                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTg                                                                                                 >  2:415154/1‑151 (MQ=255)
                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGGCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCGTTGTGCATTTTACGGAAtgtt                                                                                                  >  1:835423/1‑150 (MQ=255)
                                                tGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGt                                                                                                >  1:980772/1‑150 (MQ=255)
                                                 gCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACGGAGGCTGCGGAAGCTAACATCGGAACCCTGCGCCGGACCGCGGTAACGCCTTTTGTGCATTTTAGGGAAttt                                                                                                   >  2:288531/1‑146 (MQ=255)
                                                 gCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTAc                                                                                              <  2:1462474/151‑1 (MQ=255)
                                                  cACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACg                                                                                             >  2:176562/1‑151 (MQ=255)
                                                    cGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtt                                                                                                              <  3:246875/132‑1 (MQ=255)
                                                    cGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCt                                                                                           >  2:1048067/1‑151 (MQ=255)
                                                      ggTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCttt                                                                                         >  2:350377/1‑151 (MQ=255)
                                                         cATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTgg                                                                                       <  1:1668715/150‑1 (MQ=255)
                                                         cATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGt                                                                                      >  1:1259643/1‑151 (MQ=255)
                                                          aTAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGtt                                                                                     <  2:255459/151‑1 (MQ=255)
                                                           tAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTg                                                                                    >  3:585079/1‑151 (MQ=255)
                                                            aGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTg                                                                                    <  4:248240/150‑1 (MQ=255)
                                                            aGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGt                                                                                   <  2:1424511/151‑1 (MQ=255)
                                                            aGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGt                                                                                   <  3:328198/151‑1 (MQ=255)
                                                             gCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGt                                                                                   >  4:421452/1‑150 (MQ=255)
                                                             gCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTa                                                                                  <  2:859229/151‑1 (MQ=255)
                                                              cACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTa                                                                                  >  2:595907/1‑150 (MQ=255)
                                                                cgacgTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACa                                                                               <  4:100658/151‑1 (MQ=255)
                                                                  acgTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATc                                                                             <  2:1184022/151‑1 (MQ=255)
                                                                   cgTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCa                                                                            <  2:1433515/151‑1 (MQ=255)
                                                                    gTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATc                                                                             >  3:94405/1‑149 (MQ=255)
                                                                    gTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAg                                                                           <  3:421472/151‑1 (MQ=255)
                                                                      gctgctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGc                                                                          <  2:518378/150‑1 (MQ=255)
                                                                          ctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGc                                                                     >  2:1041628/1‑151 (MQ=255)
                                                                              gATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCt                                                                 >  1:359183/1‑151 (MQ=255)
                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTcc                                                                  <  2:1645967/145‑1 (MQ=255)
                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTa                                                               <  2:1410772/148‑1 (MQ=255)
                                                                                    aCGGGCAGTTAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTa                                                           <  4:469091/151‑1 (MQ=255)
                                                                                    aCGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAttt                                                            >  1:990809/1‑150 (MQ=255)
                                                                                       ggCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACgg                                                        <  1:1729852/151‑1 (MQ=255)
                                                                                             cAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTa                                                  <  2:1677152/151‑1 (MQ=255)
                                                                                               gacgacCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACg                                                <  1:985607/151‑1 (MQ=255)
                                                                                                  gacCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctg                                             <  2:230618/151‑1 (MQ=255)
                                                                                                    ccACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctgct                                           >  2:643193/1‑151 (MQ=255)
                                                                                                     cACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctgctg                                          >  1:1533577/1‑151 (MQ=255)
                                                                                                         gCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCttt                                      <  2:1301575/151‑1 (MQ=255)
                                                                                                          ccAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCtttt                                     >  2:19451/1‑151 (MQ=255)
                                                                                                          ccAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCtttt                                     >  4:425802/1‑151 (MQ=255)
                                                                                                           cAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCttttt                                    <  4:555222/151‑1 (MQ=255)
                                                                                                            aaCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTa                                   <  2:31371/151‑1 (MQ=255)
                                                                                                             aCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAg                                  <  1:1047939/151‑1 (MQ=255)
                                                                                                             aCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAg                                  <  1:516898/151‑1 (MQ=255)
                                                                                                                gCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCt                               <  1:473888/151‑1 (MQ=255)
                                                                                                                  tttCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCt                               <  1:135409/149‑1 (MQ=255)
                                                                                                                   ttCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGa                             <  3:485228/150‑1 (MQ=255)
                                                                                                                   ttCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGa                             <  3:293436/150‑1 (MQ=255)
                                                                                                                     cAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGagca                          <  2:471694/151‑1 (MQ=255)
                                                                                                                          cGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGTCCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCgg                     <  4:213922/151‑1 (MQ=255)
                                                                                                                          cGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCgg                     <  1:680516/151‑1 (MQ=255)
                                                                                                                            aaGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGtt                   <  2:274989/151‑1 (MQ=255)
                                                                                                                               cTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGttttt                >  2:667214/1‑151 (MQ=255)
                                                                                                                                tGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTc               >  2:1219365/1‑151 (MQ=255)
                                                                                                                                 gCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTcc              >  2:875673/1‑151 (MQ=255)
                                                                                                                                     aaGCTAACATCCGCTCCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTTCGCTTTGGTTGTAACATCAGCGACTCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCg             <  1:833698/148‑1 (MQ=255)
                                                                                                                                        cTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGgttgtt       >  3:108749/1‑151 (MQ=255)
                                                                                                                                         tAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTc      >  1:1562759/1‑151 (MQ=255)
                                                                                                                                          aaCATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCa     <  1:331062/151‑1 (MQ=255)
                                                                                                                                             aTCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAAca  <  2:415615/151‑1 (MQ=255)
                                                                                                                                             aTCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAAca  <  1:1667801/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTTGTTCAACA  >  NC_000913/3448954‑3449245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑