Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 449,925 A→G I239I (ATT→ATC cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913449,9250AG99.2% 523.0 / NA 134I239I (ATT→ATCcyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (68/65);  total (68/66)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GTAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATC  >  NC_000913/449778‑450072
                                                                                                                                                     |                                                                                                                                                   
gTAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATc                                                                                                                                                  <  2:402564/151‑1 (MQ=255)
 tAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATc                                                                                                                                                  >  2:515689/1‑150 (MQ=255)
  aaaCTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATc                                                                                                                                                  >  1:11006/1‑149 (MQ=255)
  aaaCTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATc                                                                                                                                                  >  4:242311/1‑149 (MQ=255)
    aCTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTa                                                                                                                                              <  2:293470/151‑1 (MQ=255)
    aCTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTa                                                                                                                                              <  3:89440/151‑1 (MQ=255)
       tCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACg                                                                                                                                            <  3:92409/150‑1 (MQ=255)
         cGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTc                                                                                                                                         >  1:1121251/1‑151 (MQ=255)
           ggaggCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGAGCCCAGATAGCGAGCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTcgc                                                                                                                                       <  1:45756/148‑1 (MQ=255)
              ggCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCgcgc                                                                                                                                     >  1:461572/1‑150 (MQ=255)
              ggCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCgcgc                                                                                                                                     <  1:1364562/150‑1 (MQ=255)
               gCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGcac                                                                                                                                   <  1:1708042/151‑1 (MQ=255)
                ccccAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGcaca                                                                                                                                  <  2:1091194/151‑1 (MQ=255)
                  ccAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGt                                                                                                                                <  2:1547736/151‑1 (MQ=255)
                  ccAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGt                                                                                                                                <  2:353380/151‑1 (MQ=255)
                     ggCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGAt                                                                                                                             <  1:1119000/151‑1 (MQ=255)
                      gCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATg                                                                                                                            <  2:1641006/151‑1 (MQ=255)
                      gCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATg                                                                                                                            >  1:563895/1‑151 (MQ=255)
                       cccAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGc                                                                                                                           <  3:128767/151‑1 (MQ=255)
                        ccAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGcc                                                                                                                          >  4:346532/1‑151 (MQ=255)
                        ccAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGcc                                                                                                                          >  2:376392/1‑151 (MQ=255)
                         cAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGccc                                                                                                                         >  2:1303764/1‑151 (MQ=255)
                          aaaTCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGccc                                                                                                                         >  1:1150625/1‑150 (MQ=255)
                          aaaTCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGccc                                                                                                                         >  1:255372/1‑150 (MQ=255)
                          aaaTCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGccc                                                                                                                         >  2:1640035/1‑150 (MQ=255)
                          aaaTCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGccc                                                                                                                         >  2:1186893/1‑150 (MQ=255)
                           aaTCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAg                                                                                                                       <  2:1234402/151‑1 (MQ=255)
                              cAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTa                                                                                                                    <  3:324930/151‑1 (MQ=255)
                               aGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTa                                                                                                                    >  2:1477808/1‑150 (MQ=255)
                               aGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTa                                                                                                                    >  1:424564/1‑150 (MQ=255)
                                ggTTGTTGTACAACATCATGTTTCCACCCATATCGTTTGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTa                                                                                                                    >  1:1077445/1‑149 (MQ=255)
                                ggTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTaa                                                                                                                   >  2:772602/1‑150 (MQ=255)
                                   tGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATa                                                                                                                <  1:1048163/150‑1 (MQ=255)
                                     aTGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGTTCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTg                                                                                                             <  1:1312046/151‑1 (MQ=255)
                                        tACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGc                                                                                                           >  2:1624710/1‑150 (MQ=255)
                                         aCATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCAt                                                                                                         <  2:183002/151‑1 (MQ=255)
                                         aCATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCAt                                                                                                         <  2:1565727/151‑1 (MQ=255)
                                         aCATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCAt                                                                                                         <  2:335191/151‑1 (MQ=255)
                                         aCATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCAt                                                                                                         <  2:1436053/151‑1 (MQ=255)
                                           atcatcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCt                                                                                                       >  1:48005/1‑151 (MQ=255)
                                            tcatcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCtt                                                                                                      >  2:655675/1‑151 (MQ=255)
                                             catcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTg                                                                                                     <  1:1723519/151‑1 (MQ=255)
                                              atcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGa                                                                                                    <  4:563942/151‑1 (MQ=255)
                                               tcatGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGa                                                                                                    <  2:554973/150‑1 (MQ=255)
                                                   gTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATg                                                                                               <  2:255414/151‑1 (MQ=255)
                                                    ttGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATgg                                                                                              >  3:547584/1‑151 (MQ=255)
                                                     tGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATgg                                                                                              <  1:165864/150‑1 (MQ=255)
                                                       ccACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTc                                                                                            <  1:796712/150‑1 (MQ=255)
                                                       ccACCCATTCCGTGGTAAAGGAATTGGTGCCCAGGAAGCCATCCAGGGTTAACAACGGGGACGTTACCGGTCGAATTGGGAAAGAAAGAAATAACCGTACCGTCCCGGACCGGTATTGCCAGGTAAATACTGGCATCtt                                                                                                      <  1:516644/139‑1 (MQ=255)
                                                        cACCCATATCGTTGGTAAAGAAATGGGCGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCAt                                                                                          >  1:1417274/1‑151 (MQ=255)
                                                            cATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGccc                                                                                      <  3:489209/151‑1 (MQ=255)
                                                               atCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGt                                                                                   <  1:1673749/151‑1 (MQ=255)
                                                                tCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTg                                                                                  >  4:158053/1‑151 (MQ=255)
                                                                   ttGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgcgc                                                                               >  2:1355651/1‑151 (MQ=255)
                                                                   ttGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgcgcg                                                                              >  3:257685/1‑151 (MQ=255)
                                                                    tGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTgcgcg                                                                              >  4:138279/1‑151 (MQ=255)
                                                                    tGGTAAAGAAATGGGTGCCAAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCACGGTgcg                                                                                >  1:51333/1‑149 (MQ=255)
                                                                       tAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCa                                                                            >  1:7211/1‑150 (MQ=255)
                                                                        aaaGAACTGGGGGCCCAGATAGCGATCCAGGGTCAACAACGCCACGGTAACCGACAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATCCTGGCATCTTGAACCTGGTCATGCCCGGAGCGCGCATc                                                                          >  4:343152/1‑151 (MQ=255)
                                                                           gAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTc                                                                       >  1:215922/1‑151 (MQ=255)
                                                                            aaaTGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTc                                                                       >  2:1363389/1‑150 (MQ=255)
                                                                            aaaTGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCa                                                                      <  2:306140/151‑1 (MQ=255)
                                                                            aaaTGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCa                                                                      <  2:1545413/151‑1 (MQ=255)
                                                                              aTGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCGGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGa                                                                    <  1:312706/151‑1 (MQ=255)
                                                                               tGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGa                                                                    >  1:1639767/1‑150 (MQ=255)
                                                                                gggTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAAt                                                                  >  3:172011/1‑151 (MQ=255)
                                                                                 ggTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATg                                                                 <  2:1668156/151‑1 (MQ=255)
                                                                                   tGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGt                                                               >  1:776309/1‑151 (MQ=255)
                                                                                     cccAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTaa                                                             <  4:488391/151‑1 (MQ=255)
                                                                                     cccAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTa                                                              >  2:907683/1‑150 (MQ=255)
                                                                                       cAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACg                                                           <  1:1101292/151‑1 (MQ=255)
                                                                                           tAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTAAGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTaatg                                                           >  1:367782/1‑145 (MQ=255)
                                                                                            aGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAGTGGTAACgaagaa                                                      <  1:256208/151‑1 (MQ=255)
                                                                                            aGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACgaaga                                                       >  1:344657/1‑150 (MQ=255)
                                                                                              cGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGt                                                    <  1:1477649/151‑1 (MQ=255)
                                                                                               gATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGtt                                                   >  2:772628/1‑151 (MQ=255)
                                                                                                 tCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTg                                                  >  3:367966/1‑150 (MQ=255)
                                                                                                  ccAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCTCGCACAGTGATGCCCATGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCCCCTTCAGAATAGTAACGTAGAAGTTGa                                                 <  1:177384/150‑1 (MQ=255)
                                                                                                  ccAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGAt                                                <  4:391666/151‑1 (MQ=255)
                                                                                                   cAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGAt                                                >  2:677499/1‑150 (MQ=255)
                                                                                                     gggTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATAcc                                             <  1:640195/151‑1 (MQ=255)
                                                                                                      ggTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCg                                            <  3:158074/151‑1 (MQ=255)
                                                                                                         caacaaCGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGt                                          >  2:496857/1‑150 (MQ=255)
                                                                                                         caacaaCGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGt                                          <  2:464524/150‑1 (MQ=255)
                                                                                                           acaacGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAg                                       <  2:1031975/151‑1 (MQ=255)
                                                                                                              acGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGcgt                                    <  2:653151/151‑1 (MQ=255)
                                                                                                                 gcgACGGTAACCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGcgtcg                                  <  1:376340/151‑1 (MQ=255)
                                                                                                                         aaCCGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCtata                          <  3:18855/151‑1 (MQ=255)
                                                                                                                           ccGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATAcc                        >  3:534355/1‑151 (MQ=255)
                                                                                                                            cGTCAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCg                       >  1:160355/1‑151 (MQ=255)
                                                                                                                               cAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGAt                    <  2:1325649/151‑1 (MQ=255)
                                                                                                                               cAGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCAAGGTAAAAACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGAt                    >  4:111091/1‑151 (MQ=255)
                                                                                                                                aGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGAt                    >  1:1143191/1‑150 (MQ=255)
                                                                                                                                aGAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATa                   <  2:272094/151‑1 (MQ=255)
                                                                                                                                 gAATTGGGAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGAt                    >  2:1459275/1‑149 (MQ=255)
                                                                                                                                      gggAAGGAAGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTgg              <  2:7212/150‑1 (MQ=255)
                                                                                                                                       ggaaggaaGCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGgag            <  2:1060565/151‑1 (MQ=255)
                                                                                                                                        gaaggaagCACTGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGCACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTgg              >  1:708532/1‑148 (MQ=255)
                                                                                                                                          aaggaagCAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGAc          <  2:1077575/151‑1 (MQ=255)
                                                                                                                                                 cAATGATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCata    <  1:907570/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGGGTCAACAACGCGACGGTAACCGTCAGAATTGGGAAGGAAGCAATAATCAGTACGTTCGCGCACAGTGATGCCCAGGTAAATACTGGCATCTTGAACATGGTCATGCCCGGTGCGCGCATCTTCAGAATGGTAACGAAGAAGTTGATACCGGTAAGCGTCGTACCTATACCGGATAGCTGGAGACTCCATATC  >  NC_000913/449778‑450072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: