Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,612,540 T→C W226R (TGG→CGG)  yhhT → UPF0118 family putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,612,5400TC100.0% 477.0 / NA 128W226R (TGG→CGG) yhhTUPF0118 family putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/69);  total (59/69)

Only 100 of 129 total aligned reads displayed.

AATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTCATCG  >  NC_000913/3612396‑3612690
                                                                                                                                                |                                                                                                                                                      
aatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGc                                                                                                                                                    >  3:552719/1‑149 (MQ=255)
aatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCa                                                                                                                                                   <  4:375395/150‑1 (MQ=255)
aatCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAg                                                                                                                                                  <  4:458158/151‑1 (MQ=255)
   ccACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTAc                                                                                                                                               >  2:1033401/1‑151 (MQ=255)
    cacaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGTTGCAGTTTGCGCTGATGCGGGCAGTACt                                                                                                                                              <  1:1076788/151‑1 (MQ=255)
    cacaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACt                                                                                                                                              <  3:292728/151‑1 (MQ=255)
     acaGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTg                                                                                                                                             <  1:420914/151‑1 (MQ=255)
      caGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTgg                                                                                                                                            >  2:36967/1‑151 (MQ=255)
      caGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTgg                                                                                                                                            <  1:364132/151‑1 (MQ=255)
      caGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATATTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTgg                                                                                                                                            >  1:1714246/1‑151 (MQ=255)
        gATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCg                                                                                                                                          >  3:208277/1‑151 (MQ=255)
         aTTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGt                                                                                                                                         <  1:458053/151‑1 (MQ=255)
         aTTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGt                                                                                                                                         <  2:431667/151‑1 (MQ=255)
         aTTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGt                                                                                                                                         >  2:158894/1‑151 (MQ=255)
          ttCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGtt                                                                                                                                        >  1:299418/1‑151 (MQ=255)
          ttCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGtt                                                                                                                                        >  2:892485/1‑151 (MQ=255)
          ttCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGtt                                                                                                                                        >  1:1609115/1‑151 (MQ=255)
            cacaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGtttt                                                                                                                                      >  1:1107033/1‑151 (MQ=255)
            cacaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGttt                                                                                                                                       <  2:581460/150‑1 (MQ=255)
             acaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGttttt                                                                                                                                     <  4:206230/151‑1 (MQ=255)
             acaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGttttt                                                                                                                                     <  2:889492/151‑1 (MQ=255)
                    gggATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAAc                                                                                                                              <  2:375556/151‑1 (MQ=255)
                     ggATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACt                                                                                                                             <  1:44579/151‑1 (MQ=255)
                      gATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTa                                                                                                                            <  3:581582/151‑1 (MQ=255)
                         tACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACg                                                                                                                          <  2:1534651/150‑1 (MQ=255)
                          acacCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGTTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGt                                                                                                                         <  2:327592/150‑1 (MQ=255)
                             ccGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGccc                                                                                                                     <  3:550429/151‑1 (MQ=255)
                               gcgcACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCa                                                                                                                    >  3:363705/1‑150 (MQ=255)
                                 gcACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAat                                                                                                                  >  1:1137757/1‑150 (MQ=255)
                                    cTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCg                                                                                                              >  2:1284425/1‑151 (MQ=255)
                                     ttAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCgg                                                                                                             >  2:1157813/1‑151 (MQ=255)
                                      tAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCTAATATCgg                                                                                                             <  1:510700/150‑1 (MQ=255)
                                      tAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCgg                                                                                                             <  1:923120/150‑1 (MQ=255)
                                      tAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCgg                                                                                                             >  1:1152932/1‑150 (MQ=255)
                                      tAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCg                                                                                                              <  3:179625/149‑1 (MQ=255)
                                       aaaGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGgcg                                                                                                           <  3:41719/151‑1 (MQ=255)
                                        aaGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGgcgc                                                                                                          >  2:669348/1‑151 (MQ=255)
                                        aaGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGgcgc                                                                                                          >  1:1302001/1‑151 (MQ=255)
                                          ggCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCgg                                                                                                        <  4:589688/151‑1 (MQ=255)
                                           gCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGt                                                                                                       <  2:1432730/151‑1 (MQ=255)
                                             gTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTa                                                                                                      >  2:1660993/1‑150 (MQ=255)
                                              tttCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTaa                                                                                                     >  1:450264/1‑150 (MQ=255)
                                                tCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAtt                                                                                                   <  1:1255695/150‑1 (MQ=255)
                                                tCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAtt                                                                                                   <  1:1315486/150‑1 (MQ=255)
                                                   cACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccg                                                                                               >  4:347348/1‑151 (MQ=255)
                                                   cACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccg                                                                                               >  4:6433/1‑151 (MQ=255)
                                                      tATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgccg                                                                                            >  2:750973/1‑151 (MQ=255)
                                                      tATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTATGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccgccg                                                                                            >  1:1463480/1‑151 (MQ=255)
                                                           tGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTa                                                                                          <  2:1164658/148‑1 (MQ=255)
                                                           tGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCg                                                                                       >  1:780954/1‑151 (MQ=255)
                                                            gCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCg                                                                                       <  3:3630/150‑1 (MQ=255)
                                                            gCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGc                                                                                      <  2:149316/151‑1 (MQ=255)
                                                              aTTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCa                                                                                    <  1:1438790/151‑1 (MQ=255)
                                                                tGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCaa                                                                                   <  2:1313455/150‑1 (MQ=255)
                                                                tGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCaa                                                                                   <  2:173463/150‑1 (MQ=255)
                                                                   aGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAt                                                                               >  2:1033324/1‑151 (MQ=255)
                                                                    gACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAtt                                                                              >  4:457307/1‑151 (MQ=255)
                                                                    gACGCTACTCAGTTTATGGACAGGCGTCATCGTCTGGCTGGGGCTGGAGCTGTTGGGTGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGGGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAtt                                                                              >  1:1100383/1‑151 (MQ=255)
                                                                     aCGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTc                                                                             >  2:330731/1‑151 (MQ=255)
                                                                      cGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTc                                                                             >  1:1107012/1‑150 (MQ=255)
                                                                      cGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCa                                                                            <  2:632236/151‑1 (MQ=255)
                                                                          aCTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtg                                                                        >  3:430034/1‑151 (MQ=255)
                                                                             cAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtgctg                                                                     <  1:1631641/151‑1 (MQ=255)
                                                                               gTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTgctgct                                                                   <  3:478855/151‑1 (MQ=255)
                                                                                tttATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGctgctg                                                                  >  2:1111092/1‑151 (MQ=255)
                                                                                 ttATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGctgctg                                                                  <  1:1233314/150‑1 (MQ=255)
                                                                                  tATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGt                                                                 <  2:276465/150‑1 (MQ=255)
                                                                                   aTGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGttt                                                               <  2:1714413/151‑1 (MQ=255)
                                                                                    tGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGttt                                                               >  2:155918/1‑150 (MQ=255)
                                                                                     ggACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTaa                                                             <  3:29379/151‑1 (MQ=255)
                                                                                      gACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAAt                                                            <  2:1446627/151‑1 (MQ=255)
                                                                                        cAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCCTACCGCCAATGATTCAGGTGCTGCTGTTTAATgg                                                          >  4:427444/1‑151 (MQ=255)
                                                                                          ggTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATgg                                                          <  1:27255/149‑1 (MQ=255)
                                                                                            tgtCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGgtg                                                        >  1:139389/1‑149 (MQ=255)
                                                                                             gtCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGttt                                                     <  2:1398834/151‑1 (MQ=255)
                                                                                              tCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGttt                                                     >  4:264491/1‑150 (MQ=255)
                                                                                                aTCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACg                                                  >  2:1528181/1‑151 (MQ=255)
                                                                                                     ctggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGt                                             >  1:1473389/1‑151 (MQ=255)
                                                                                                      tggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATg                                              >  1:1362972/1‑149 (MQ=255)
                                                                                                      tggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGt                                             >  2:1291949/1‑150 (MQ=255)
                                                                                                            gggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTgg                                      >  1:115875/1‑151 (MQ=255)
                                                                                                            gggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTgg                                      <  2:404301/151‑1 (MQ=255)
                                                                                                            gggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTgg                                      >  4:272213/1‑151 (MQ=255)
                                                                                                                 ggtgCTGATGGGGGTGCGTTTTGCGATGTTGCGGGCCCTACTGGCGTTTTTGCCCAACTACTTCCCCAATATGGTTGCGGTAATTTCCGCCGTTCCGCAAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCgg                                  <  1:932004/147‑1 (MQ=255)
                                                                                                                 ggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCg                                   <  2:1107168/149‑1 (MQ=255)
                                                                                                                 ggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGgc                                 >  2:481284/1‑151 (MQ=255)
                                                                                                                  gAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGgcg                                <  2:1058580/151‑1 (MQ=255)
                                                                                                                     cTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCAt                             >  2:913132/1‑151 (MQ=255)
                                                                                                                      ttaTGGGGATGCGGTTTGAGCTGATGCGGGCAGTACTGGCGTTTTAGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGCTTACGGATGTATTCTGGTCGgc                                 >  1:1373529/3‑146 (MQ=255)
                                                                                                                           ggggTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGtttt                       <  1:1534126/151‑1 (MQ=255)
                                                                                                                                gCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGt                   <  4:511736/150‑1 (MQ=255)
                                                                                                                                         gcTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTGCGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATg         <  4:565930/151‑1 (MQ=255)
                                                                                                                                         gcTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATg         <  1:387535/151‑1 (MQ=255)
                                                                                                                                          cTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATgg        >  1:208003/1‑151 (MQ=255)
                                                                                                                                           tGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGt       >  3:62050/1‑151 (MQ=255)
                                                                                                                                            gATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATgg        <  4:397280/149‑1 (MQ=255)
                                                                                                                                            gATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTc      >  2:573153/1‑151 (MQ=255)
                                                                                                                                             aTGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTCa     <  3:264509/151‑1 (MQ=255)
                                                                                                                                             aTGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTCa     <  2:881047/151‑1 (MQ=255)
                                                                                                                                                tgGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGCTTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTCATCg  >  1:1627623/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                      
AATCCACAGATTCACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCCATATGGTCATCG  >  NC_000913/3612396‑3612690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑