Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,992,016 T→C R288R (CGT→CGC cyaA → adenylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,992,0160TC100.0% 559.6 / NA 147R288R (CGT→CGCcyaAadenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (74/73);  total (74/73)

Only 100 of 149 total aligned reads displayed.

TGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTC  >  NC_000913/3991869‑3992163
                                                                                                                                                   |                                                                                                                                                   
tgctgAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTg                                                                                                                                                  >  2:115357/1‑151 (MQ=255)
tgctgAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTg                                                                                                                                                  >  3:119898/1‑151 (MQ=255)
  ctgAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGc                                                                                                                                                 >  2:1179479/1‑150 (MQ=255)
  ctgAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGc                                                                                                                                                 >  2:1720989/1‑150 (MQ=255)
      agagTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCacgac                                                                                                                                            >  3:9655/1‑151 (MQ=255)
       gagTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCacgacg                                                                                                                                           <  1:283186/151‑1 (MQ=255)
            cTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCg                                                                                                                                        >  3:177452/1‑149 (MQ=255)
             tttGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCg                                                                                                                                        >  1:1681432/1‑148 (MQ=255)
             tttGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCg                                                                                                                                        >  2:562308/1‑148 (MQ=255)
               tGGTGCCAGCCTTTGGCAGCTCTACGAGAGTATGGATTCCCCATACAAAGTGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTCGGAATACCCGAACCCACGTCTGCTGGCGCAAGTTATCAAACAGCGCTTGGACGACGGCGAGAtt                                                                                                                                   >  1:526228/1‑151 (MQ=255)
                ggTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGAtt                                                                                                                                   <  4:48976/150‑1 (MQ=255)
                ggTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGAtt                                                                                                                                   <  1:253037/150‑1 (MQ=255)
                ggTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGAtt                                                                                                                                   <  4:186106/150‑1 (MQ=255)
                    ccAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCGCGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCGCGACGGCGAGATTGTATc                                                                                                                              <  1:927457/151‑1 (MQ=255)
                    ccAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATc                                                                                                                              <  2:91049/151‑1 (MQ=255)
                    ccAGCCTTTGGCAGCTCTACAAGAGTATCGAGTGCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGAGAGATTGTATc                                                                                                                              >  4:245976/1‑151 (MQ=255)
                     cAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCg                                                                                                                             >  2:1147094/1‑151 (MQ=255)
                     cAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCg                                                                                                                             >  2:295840/1‑151 (MQ=255)
                         cTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTg                                                                                                                         >  1:107510/1‑151 (MQ=255)
                           ttGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTg                                                                                                                         <  2:819715/149‑1 (MQ=255)
                              gCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGtctc                                                                                                                    <  1:251559/151‑1 (MQ=255)
                              gCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGtctc                                                                                                                    <  1:1324781/151‑1 (MQ=255)
                               cAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCg                                                                                                                   >  3:478728/1‑151 (MQ=255)
                                aGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCg                                                                                                                   >  1:562562/1‑150 (MQ=255)
                                aGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGa                                                                                                                  <  2:1203172/151‑1 (MQ=255)
                                aGCTCTACAAGAGAATCGATTCCCCATACAAAGCGGTACTGCAAACACTGCTGCTGGAAGCCTAATCCTGGCAATACCCGCACCCACGTATGCTGGCGAAAGATATCATTCAGCGCTTGCACGACGGCGAGATTGTCTCGTTTGGtc                                                                                                                      >  1:1318312/1‑147 (MQ=255)
                                   tctACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATcc                                                                                                               >  4:454841/1‑151 (MQ=255)
                                    ctACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATc                                                                                                                <  1:341412/149‑1 (MQ=255)
                                    ctACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATc                                                                                                                <  4:450741/149‑1 (MQ=255)
                                        aaGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACt                                                                                                          >  2:258736/1‑151 (MQ=255)
                                        aaGAGTATCGATTCCCCATACAAAGCGGTACGGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATTCCCGAACCCTCGTCTGGTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACt                                                                                                          <  1:1374014/151‑1 (MQ=255)
                                              aTCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCatg                                                                                                     <  2:1292160/150‑1 (MQ=255)
                                                     ccccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTgg                                                                                              >  2:1736236/1‑150 (MQ=255)
                                                      cccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTgg                                                                                              >  4:230124/1‑149 (MQ=255)
                                                      cccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAg                                                                                            <  2:1224410/151‑1 (MQ=255)
                                                         aTACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCgt                                                                                         <  1:1462956/151‑1 (MQ=255)
                                                         aTACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCgt                                                                                         <  1:1488458/151‑1 (MQ=255)
                                                           aCAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCgtgt                                                                                       <  3:454858/151‑1 (MQ=255)
                                                           aCAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCgtgt                                                                                       >  1:667789/1‑151 (MQ=255)
                                                           aCAAAGCGGTACTAAAAACATTGCTGCAGGAAGCCTATTCCTGAGCATACTCGCACCCACGTCTGCTGGCGAAAGATATCAAACCGCGCTTGCACGACGGCGAGATTGTATCGTTTGAACTCGATGCATACTGCATGATGCTGGAGCg                                                                                          >  1:1580262/1‑148 (MQ=255)
                                                            cAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGtt                                                                                      >  2:205852/1‑151 (MQ=255)
                                                             aaaGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTa                                                                                     <  1:440886/151‑1 (MQ=255)
                                                              aaGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTAc                                                                                    >  2:742727/1‑151 (MQ=255)
                                                                  ggTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGa                                                                                 >  2:836953/1‑150 (MQ=255)
                                                                   gTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGaa                                                                                >  1:837253/1‑150 (MQ=255)
                                                                   gTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAAt                                                                               <  2:632680/151‑1 (MQ=255)
                                                                     aCTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATAc                                                                             <  4:362977/151‑1 (MQ=255)
                                                                     aCTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATAc                                                                             >  1:470283/1‑151 (MQ=255)
                                                                        gAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATAcc                                                                            <  2:1425887/149‑1 (MQ=255)
                                                                        gAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCt                                                                           <  4:316101/150‑1 (MQ=255)
                                                                          aaaCACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGAc                                                                        <  1:605465/151‑1 (MQ=255)
                                                                             cacTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                     <  1:8220/151‑1 (MQ=255)
                                                                             cacTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                     >  2:1100840/1‑151 (MQ=255)
                                                                             cacTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGc                                                                     <  2:1046499/151‑1 (MQ=255)
                                                                                tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGAt                                                                  >  4:580895/1‑151 (MQ=255)
                                                                                    gctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGa                                                               >  4:407925/1‑150 (MQ=255)
                                                                                     ctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGa                                                               >  1:302092/1‑149 (MQ=255)
                                                                                      tgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                >  1:618600/1‑147 (MQ=255)
                                                                                       ggAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGa                                                            <  4:482106/150‑1 (MQ=255)
                                                                                        gAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGAtt                                                          <  1:1011200/151‑1 (MQ=255)
                                                                                        gAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGAtt                                                          <  2:279604/151‑1 (MQ=255)
                                                                                           gCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGAtttt                                                        >  1:217484/1‑150 (MQ=255)
                                                                                              tATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                    >  2:811218/1‑151 (MQ=255)
                                                                                               aTTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                    <  2:677892/150‑1 (MQ=255)
                                                                                                  ccTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                <  1:38229/151‑1 (MQ=255)
                                                                                                  ccTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                <  4:101766/151‑1 (MQ=255)
                                                                                                   cTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTg                                               >  2:66350/1‑151 (MQ=255)
                                                                                                   cTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTg                                               >  2:1629280/1‑151 (MQ=255)
                                                                                                    tGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                              >  3:299803/1‑151 (MQ=255)
                                                                                                     gggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                              <  1:339365/150‑1 (MQ=255)
                                                                                                      ggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGAt                                            <  1:966586/151‑1 (MQ=255)
                                                                                                      ggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGAt                                            <  2:249063/151‑1 (MQ=255)
                                                                                                       gAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGAtt                                           <  4:421864/151‑1 (MQ=255)
                                                                                                           aCCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGt                                       >  1:1660482/1‑151 (MQ=255)
                                                                                                             ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTAc                                     >  1:834607/1‑151 (MQ=255)
                                                                                                             ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTAc                                     >  1:753101/1‑151 (MQ=255)
                                                                                                              cGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACg                                    >  1:139651/1‑151 (MQ=255)
                                                                                                              cGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACg                                    >  2:721464/1‑151 (MQ=255)
                                                                                                               gAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGt                                   >  1:1691308/1‑151 (MQ=255)
                                                                                                               gAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGt                                   >  2:220270/1‑151 (MQ=255)
                                                                                                               gAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGt                                   >  3:255794/1‑151 (MQ=255)
                                                                                                                   ccACGTCTGCTGGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgcg                               <  1:1146959/151‑2 (MQ=255)
                                                                                                                          tgctgGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCt                         >  1:1026859/1‑150 (MQ=255)
                                                                                                                           gctgGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTAt                       <  4:162602/151‑1 (MQ=255)
                                                                                                                             tgGCGAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTAttt                     >  2:148982/1‑151 (MQ=255)
                                                                                                                                 gAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaaa                 <  2:301898/151‑1 (MQ=255)
                                                                                                                                 gAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaaa                 <  1:1720819/151‑1 (MQ=255)
                                                                                                                                 gAAAGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaa                  >  2:1575494/1‑150 (MQ=255)
                                                                                                                                  aaaGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaa                  >  2:357549/1‑149 (MQ=255)
                                                                                                                                  aaaGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAg                <  2:934427/151‑1 (MQ=255)
                                                                                                                                   aaGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaaa                 <  1:1157075/149‑1 (MQ=255)
                                                                                                                                   aaGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgt               >  2:1272892/1‑151 (MQ=255)
                                                                                                                                   aaGATATCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgt               >  1:381862/1‑151 (MQ=255)
                                                                                                                                       tatCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGtgtg            >  1:538177/1‑150 (MQ=255)
                                                                                                                                       tatCAAACAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTgcgc           >  1:232301/1‑151 (MQ=255)
                                                                                                                                           aaaCAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGaaa       <  1:1195699/151‑1 (MQ=255)
                                                                                                                                            aaCAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGaaa       >  1:1196180/1‑150 (MQ=255)
                                                                                                                                             aCAGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAg     <  1:1734164/151‑1 (MQ=255)
                                                                                                                                               aGCGCTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAg     >  2:588050/1‑149 (MQ=255)
                                                                                                                                                gcgcTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTc  >  4:573066/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCTGAAGAGTACTTTGGTGCCAGCCTTTGGCAGCTCTACAAGAGTATCGATTCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTC  >  NC_000913/3991869‑3992163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: