BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	482,315	G→A	Q697* (CAG→TAG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	482,315	0	G	A	100.0%	546.9 / NA	134	Q697* (CAG→TAG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (62/72); total (62/72)

Only 100 of 134 total aligned reads displayed.

GTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGG  >  NC_000913/482169‑482461

    ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGtt                                                                                                                                            >  2:1281487/1‑151 (MQ=255)

     tGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGttt                                                                                                                                           >  1:1093254/1‑151 (MQ=255)

     tGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGtt                                                                                                                                            <  1:1125397/150‑1 (MQ=255)

        tAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTc                                                                                                                                        >  1:122253/1‑151 (MQ=255)

         aGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTc                                                                                                                                        <  1:1647031/150‑1 (MQ=255)

           aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGt                                                                                                                                      <  1:476212/150‑1 (MQ=255)

            aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGa                                                                                                                                    <  2:1473371/151‑1 (MQ=255)

                 ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCa                                                                                                                                 >  2:362362/1‑149 (MQ=255)

                 ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCa                                                                                                                                 >  2:1720524/1‑149 (MQ=255)

                  cAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAgg                                                                                                                              <  1:1599351/151‑1 (MQ=255)

                    gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGTAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGcc                                                                                                                            <  4:511609/151‑1 (MQ=255)

                    gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGcc                                                                                                                            <  3:593194/151‑1 (MQ=255)

                    gcgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGcc                                                                                                                            <  1:772466/151‑1 (MQ=255)

                     cgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGcc                                                                                                                            >  2:878567/1‑150 (MQ=255)

                     cgcCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCa                                                                                                                           <  1:100812/151‑1 (MQ=255)

                        cTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGATGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGcc                                                                                                                        >  2:359247/1‑151 (MQ=255)

                          gcgcTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCt                                                                                                                       <  1:430692/150‑1 (MQ=255)

                            gcTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                                                                                                                    >  2:692753/1‑151 (MQ=255)

                              tttttCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTc                                                                                                                   >  2:1716333/1‑150 (MQ=255)

                               ttttCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                                                                                                  >  3:414631/1‑150 (MQ=255)

                               ttttCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                                                                                                  <  2:1726642/150‑1 (MQ=255)

                                   ccTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAg                                                                                                             <  2:796840/151‑1 (MQ=255)

                                      ggTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGc                                                                                                            <  1:342218/149‑1 (MQ=255)

                                       gTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                                                                                                         <  1:1532088/151‑1 (MQ=255)

                                         cGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                                                                                                       <  2:1053931/151‑1 (MQ=255)

                                          gATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaag                                                                                                      <  1:62917/151‑1 (MQ=255)

                                          gATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                                                                                        >  2:1110685/1‑149 (MQ=255)

                                          gATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                                                                                        >  2:1605178/1‑149 (MQ=255)

                                          gATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                                                                                                        >  2:1194016/1‑149 (MQ=255)

                                             atCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc                                                                                                    >  1:982638/1‑150 (MQ=255)

                                               cAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATAACAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtc                                                                                                    <  1:44120/148‑1 (MQ=255)

                                                aaTCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag                                                                                                <  2:621272/151‑1 (MQ=255)

                                                aaTCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag                                                                                                <  1:1456708/151‑1 (MQ=255)

                                                aaTCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa                                                                                                  >  2:1519294/1‑149 (MQ=255)

                                                  tCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                                                                                              >  1:1276444/1‑151 (MQ=255)

                                                  ccTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                                                                                              >  1:865007/2‑151 (MQ=255)

                                                   cTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg                                                                                             >  2:1600779/1‑151 (MQ=255)

                                                    ttAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg                                                                                            >  2:1435477/1‑151 (MQ=255)

                                                     tAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg                                                                                            <  2:494982/150‑1 (MQ=255)

                                                       aaCTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg                                                                                         <  4:107462/151‑1 (MQ=255)

                                                            cGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTa                                                                                    <  2:115746/151‑1 (MQ=255)

                                                             ggggTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATCTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGCCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCACAGCCGGTTGCAGTAc                                                                                   <  1:869964/151‑1 (MQ=255)

                                                             ggggTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAc                                                                                   >  1:1699347/1‑151 (MQ=255)

                                                             ggggTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAc                                                                                   >  2:1558281/1‑151 (MQ=255)

                                                              gggTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAcc                                                                                  <  2:824464/151‑1 (MQ=255)

                                                                gTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCa                                                                                <  2:288632/151‑1 (MQ=255)

                                                                     ttCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc                                                                           >  2:216285/1‑151 (MQ=255)

                                                                        cAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACg                                                                        <  1:362310/151‑1 (MQ=255)

                                                                           aCCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt                                                                     <  2:210922/151‑1 (MQ=255)

                                                                           aCCGTTTGGACGGACGCTGGTCAACATATCAGGGAGCTTCGCTGCTTCTGCAAACAACTGGTTACGCGCCTAGGTCAGTTTTTCGTGGCAAAAGCCAACCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGAACCCAGTTCCACGAtt                                                                     >  1:1204454/1‑151 (MQ=255)

                                                                            ccGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAt                                                                      <  1:1130463/149‑1 (MQ=255)

                                                                             cGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGc                                                                   >  4:304118/1‑151 (MQ=255)

                                                                              gTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCg                                                                  >  1:719730/1‑151 (MQ=255)

                                                                               tttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCgg                                                                 >  1:878328/1‑151 (MQ=255)

                                                                                ttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACTCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGctg                                                                 <  1:1050285/150‑3 (MQ=255)

                                                                                ttGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCgg                                                                 <  4:302645/150‑1 (MQ=255)

                                                                                  ggACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCggg                                                                >  1:1369226/1‑149 (MQ=255)

                                                                                  ggACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGc                                                               <  2:1687332/150‑1 (MQ=255)

                                                                                  ggACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCa                                                              <  2:26256/151‑1 (MQ=255)

                                                                                   gACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAg                                                             <  3:292720/151‑1 (MQ=255)

                                                                                    aCGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAgg                                                            <  3:380307/151‑1 (MQ=255)

                                                                                      gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGtt                                                          <  2:310442/151‑1 (MQ=255)

                                                                                         cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaaa                                                       <  2:1411408/151‑1 (MQ=255)

                                                                                         cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaa                                                        >  1:731612/1‑150 (MQ=255)

                                                                                           cTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAgg                                                     >  1:1332005/1‑151 (MQ=255)

                                                                                             ggTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                   <  1:487879/151‑1 (MQ=255)

                                                                                             ggTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCg                                                   >  2:1376663/1‑151 (MQ=255)

                                                                                              gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGa                                                  <  2:573197/151‑1 (MQ=255)

                                                                                                cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGaa                                                 >  2:1204097/1‑150 (MQ=255)

                                                                                                   cATAGCAGGGTGCTTCGCTGCTGCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGaaaa                                               <  1:1230022/149‑1 (MQ=255)

                                                                                                     tatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt                                           >  4:152141/1‑151 (MQ=255)

                                                                                                       tCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCg                                         >  3:76830/1‑151 (MQ=255)

                                                                                                        cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCg                                         <  2:408713/150‑1 (MQ=255)

                                                                                                        cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCg                                         <  2:1354909/150‑1 (MQ=255)

                                                                                                        cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGc                                        >  4:333214/1‑151 (MQ=255)

                                                                                                        cAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGc                                        >  1:35454/1‑151 (MQ=255)

                                                                                                          gggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCAt                                      <  1:1277104/151‑1 (MQ=255)

                                                                                                             tGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCtt                                   >  1:330333/1‑151 (MQ=255)

                                                                                                              gCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCtt                                   <  3:458493/150‑1 (MQ=255)

                                                                                                                ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCttt                                  <  1:660898/149‑1 (MQ=255)

                                                                                                                ttCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTg                                 >  2:1310120/1‑150 (MQ=255)

                                                                                                                 tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTg                                 <  2:9609/149‑1 (MQ=255)

                                                                                                                 tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGAt                               >  3:29432/1‑151 (MQ=255)

                                                                                                                   gctgctTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGAttt                             <  2:1123189/151‑1 (MQ=255)

                                                                                                                    ctgctTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTg                            >  1:913200/1‑151 (MQ=255)

                                                                                                                      gctTCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCg                          >  1:463923/1‑151 (MQ=255)

                                                                                                                         tCTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgag                        <  2:122274/150‑1 (MQ=255)

                                                                                                                          cTGCAAGCAACTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgag                        >  3:136692/1‑149 (MQ=255)

                                                                                                                            gcaagcaaCTGGTTACGCGCCTAAGTTAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGaa                      <  2:278247/149‑1 (MQ=255)

                                                                                                                            gcaagcaaCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCgaga                       <  1:1004049/148‑1 (MQ=255)

                                                                                                                            gcaagcaaCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGaa                      >  2:712865/1‑149 (MQ=255)

                                                                                                                             caagcaaCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgc                   <  1:216252/151‑1 (MQ=255)

                                                                                                                               agcaaCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAgcgc                 <  2:654557/151‑1 (MQ=255)

                                                                                                                                   aCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCgtgt             >  2:697036/1‑151 (MQ=255)

                                                                                                                                   aCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCgtgt             <  4:19441/151‑1 (MQ=255)

                                                                                                                                   aCTGGTTACGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCgtgt             <  4:504521/151‑1 (MQ=255)

                                                                                                                                          aCGCGCCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACg       >  2:964642/1‑150 (MQ=255)

                                                                                                                                            gcgcCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCAt    >  2:1519160/1‑151 (MQ=255)

                                                                                                                                            gcgcCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCAt    <  2:1276589/151‑1 (MQ=255)

                                                                                                                                             cgcCTAAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCAt    <  4:210721/150‑1 (MQ=255)

GTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCGCATCTTTGATTTGCGAGAAAGCGCGTGTTGCACGCATGG  >  NC_000913/482169‑482461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`