Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,302,141 T→C E313G (GAG→GGG)  mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,302,1410TC98.7% 543.4 / ‑3.5 153E313G (GAG→GGG) mdtOmembrane translocase (MDR) of MdtNOP efflux pump, PET family
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (63/88);  total (64/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 155 total aligned reads displayed.

AAACAGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCA  >  NC_000913/4301994‑4302287
                                                                                                                                                   |                                                                                                                                                  
aaaCAGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTcccc                                                                                                                                                  >  2:67620/1‑150 (MQ=255)
aaaCAGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCa                                                                                                                                                 <  1:219642/151‑1 (MQ=255)
 aaCAGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTcccc                                                                                                                                                  >  2:1138358/1‑149 (MQ=255)
    aGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGtt                                                                                                                                             <  4:586988/151‑1 (MQ=255)
     ggCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTa                                                                                                                                            <  2:299473/151‑1 (MQ=255)
     ggCGAGCAGCGGTTTTACCGCGGAACGCATATAGTCTGGATTGGTAAAAGCATCGGCGGGCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGCCCCAGTTGTAGCAACGTCTGGCAGATATTCCCCAgg                                                                                                                                               <  1:1258050/148‑1 (MQ=255)
       cGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTAc                                                                                                                                           >  2:378714/1‑150 (MQ=255)
           cagcGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTcc                                                                                                                                      <  2:1622731/151‑1 (MQ=255)
            agcGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTccc                                                                                                                                     >  2:601039/1‑151 (MQ=255)
              cGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGt                                                                                                                                   <  2:1638330/151‑1 (MQ=255)
              cGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGt                                                                                                                                   >  1:793748/1‑151 (MQ=255)
               gTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTg                                                                                                                                  <  1:200749/151‑1 (MQ=255)
               gTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTg                                                                                                                                  >  2:1220019/1‑151 (MQ=255)
               gTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTg                                                                                                                                  >  1:836927/1‑151 (MQ=255)
                tttttACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgc                                                                                                                                 >  4:191161/1‑151 (MQ=255)
                 ttttACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTgcc                                                                                                                                >  1:1297710/1‑151 (MQ=255)
                 ttttACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgcc                                                                                                                                >  2:739629/1‑151 (MQ=255)
                 ttttACCGCGTAGCGCATATACTCTGGATTGGTAAAAGCATCCGCGGCCATTGATGGCGGCTTGGCTGCGGGCGTTGGCGGCGCATTTGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgcc                                                                                                                                >  1:979004/1‑151 (MQ=255)
                   ttACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgccg                                                                                                                               <  4:437168/150‑1 (MQ=255)
                    tACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgccgcc                                                                                                                             >  3:421449/1‑151 (MQ=255)
                    tACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgccgc                                                                                                                              <  1:967498/150‑1 (MQ=255)
                     aCCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTgccgcc                                                                                                                             >  1:1720457/1‑150 (MQ=255)
                      ccGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCa                                                                                                                            >  2:1311455/1‑150 (MQ=255)
                      ccGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGCTTCGCTGCAGGCGTTGGCGGCGCATTCGTGTCCATCTGACCCAGTTTTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCa                                                                                                                            >  1:507813/1‑150 (MQ=255)
                       cgcgTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCCGTTGTAACAACGTCTGGCAGATATTCCCCAGCTTACATTCCCGTGCCGCCATc                                                                                                                          <  1:691307/151‑1 (MQ=255)
                       cgcgTAGCACATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATGGATGGCGTTTTGGCTGCGGGCGGTGGCGGCGTATTCGGGGCCGTCTGGCCCAGTTGTAACAACGTCTGGCAGGTATTCCCGATGTTACATGCCCGTGCCGCGATc                                                                                                                          <  1:70089/151‑1 (MQ=255)
                           tAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTAATCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCtt                                                                                                                      >  2:1487536/1‑151 (MQ=255)
                                 atatAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCttcacttt                                                                                                                >  1:740142/1‑151 (MQ=255)
                                      gTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTg                                                                                                           >  1:1049662/1‑151 (MQ=255)
                                      gTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTg                                                                                                           >  3:78995/1‑151 (MQ=255)
                                      gTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTg                                                                                                           <  1:997921/151‑1 (MQ=255)
                                        cTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGAt                                                                                                         >  2:389701/1‑151 (MQ=255)
                                          ggATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGAt                                                                                                         <  4:34353/149‑1 (MQ=255)
                                           gATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATcc                                                                                                       >  2:136980/1‑150 (MQ=255)
                                           gATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCg                                                                                                      <  4:296878/151‑1 (MQ=255)
                                           gATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCg                                                                                                      <  1:372187/151‑1 (MQ=255)
                                           gATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCg                                                                                                      <  4:20789/151‑1 (MQ=255)
                                              tGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGcc                                                                                                    <  2:385683/150‑1 (MQ=255)
                                               ggTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGcc                                                                                                    >  2:767541/1‑149 (MQ=255)
                                               ggTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCa                                                                                                   <  1:468469/150‑1 (MQ=255)
                                               ggTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAg                                                                                                  <  2:1478834/151‑1 (MQ=255)
                                                  aaaaGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGGTGGAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCAACGCTTCACGTTCAATGAGCCGCCAGTCg                                                                                               >  1:430619/1‑151 (MQ=255)
                                                        aTCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGc                                                                                         <  2:133459/151‑1 (MQ=255)
                                                          cggcggCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCa                                                                                       <  2:105465/151‑1 (MQ=255)
                                                          cggcggCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCa                                                                                       <  2:1737728/151‑1 (MQ=255)
                                                           ggcggcCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGcc                                                                                        >  2:1292292/1‑149 (MQ=255)
                                                                  aTTGATGGCGGTTTGGCTGCGGGCGTTGGGGGCGTATTAGGGTCCATATGACCCAGTTGTAACAACGTCTGGGAGATATTCCCCAGGGTAAATTCCCGGGCCGCCATCGGTTCACTTTCCATGAGCCGCCAGGCGCTTTGCCAGCACTGa                                                                                >  1:81068/1‑150 (MQ=255)
                                                                     gATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCtt                                                                            <  1:1294342/151‑1 (MQ=255)
                                                                      aTGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCGCTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTc                                                                           <  2:672967/151‑1 (MQ=255)
                                                                          cGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGc                                                                        >  1:596804/1‑150 (MQ=255)
                                                                           ggTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCa                                                                       >  1:474355/1‑150 (MQ=255)
                                                                            gTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAAc                                                                     <  2:168147/151‑1 (MQ=255)
                                                                                ggCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGc                                                                  <  3:356507/150‑1 (MQ=255)
                                                                                 gctgctGGCGTGGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGAGCCGCCAGGCGCTTTGCCAGCACTGCCCTTCAGCAACgg                                                                   <  1:1109450/148‑1 (MQ=255)
                                                                                 gCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCAt                                                                >  3:42043/1‑151 (MQ=255)
                                                                                   tGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACACAGTTGTAACAACGTCTGGCAGATAGTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCAtgc                                                              >  4:256673/1‑151 (MQ=255)
                                                                                    gCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCAtg                                                               <  3:183229/149‑1 (MQ=255)
                                                                                       ggCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCa                                                          <  1:1150763/151‑1 (MQ=255)
                                                                                          gTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCt                                                       >  4:386490/1‑151 (MQ=255)
                                                                                          gTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCt                                                       <  1:629387/151‑1 (MQ=255)
                                                                                          gTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCt                                                       >  1:377774/1‑151 (MQ=255)
                                                                                             ggcggcGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCttgt                                                    >  2:77952/1‑151 (MQ=255)
                                                                                             ggcggcGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCttg                                                     <  1:1267561/150‑1 (MQ=255)
                                                                                                ggcgTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCttgttg                                                  >  1:28116/1‑150 (MQ=255)
                                                                                                ggcgTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGa                                                 <  1:1328581/151‑1 (MQ=255)
                                                                                                  cgTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGAt                                                <  1:833519/150‑1 (MQ=255)
                                                                                                    tATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTc                                             >  2:1119488/1‑151 (MQ=255)
                                                                                                     aTTCGGGTTCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCt                                            <  2:619327/151‑1 (MQ=255)
                                                                                                     aTTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCt                                            >  2:1200354/1‑151 (MQ=255)
                                                                                                     aTTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCt                                            >  1:1516096/1‑151 (MQ=255)
                                                                                                     aTTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCt                                            >  1:1428620/1‑151 (MQ=255)
                                                                                                       tCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTg                                           <  3:257821/150‑1 (MQ=255)
                                                                                                        cGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGaa                                         <  1:165335/151‑1 (MQ=255)
                                                                                                             ccATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTaa                                    <  2:396220/151‑1 (MQ=255)
                                                                                                                  tGACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttt                               >  2:86482/1‑151 (MQ=255)
                                                                                                                   gACCCAGTTGTAACAACGTCTTGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGACGCTAAtt                                  <  1:1353012/147‑1 (MQ=255)
                                                                                                                   gACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAttttt                               <  2:1393198/150‑1 (MQ=255)
                                                                                                                   gACCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAAtttt                                <  1:1033688/149‑1 (MQ=255)
                                                                                                                    aCCCAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGt                             <  1:811844/151‑1 (MQ=255)
                                                                                                                     cccAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTc                            >  1:1342974/1‑151 (MQ=255)
                                                                                                                      ccAGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCg                           <  3:3333/151‑1 (MQ=255)
                                                                                                                        aGTTGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGa                         <  1:259176/151‑1 (MQ=255)
                                                                                                                          ttGTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAAt                       >  1:1651067/1‑151 (MQ=255)
                                                                                                                            gTAACAACGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAAtt                      <  2:1049790/150‑1 (MQ=255)
                                                                                                                              aacaccGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCaa                   <  1:1147695/151‑1 (MQ=255)
                                                                                                                              aacaacGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCaa                   <  2:740251/151‑1 (MQ=255)
                                                                                                                              aacaacGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCaa                   <  1:547063/151‑1 (MQ=255)
                                                                                                                              aacaacGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCa                    <  1:1517692/150‑1 (MQ=255)
                                                                                                                                  acGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCaataat               <  1:1449030/151‑1 (MQ=255)
                                                                                                                                  acGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCaataat               <  2:503491/151‑1 (MQ=255)
                                                                                                                                   cGTCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAAtt              <  1:777880/151‑1 (MQ=255)
                                                                                                                                     tCTGGCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGc            >  1:185792/1‑151 (MQ=255)
                                                                                                                                        ggCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGc            <  1:1114001/148‑1 (MQ=255)
                                                                                                                                         gCAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTga        <  1:176189/151‑1 (MQ=255)
                                                                                                                                          cAGATATTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgag       <  2:461939/151‑1 (MQ=255)
                                                                                                                                            gATATTCCCCAGGTTACATTCCGGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTgaga      <  4:570030/150‑1 (MQ=255)
                                                                                                                                               atTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATc   >  2:87986/1‑150 (MQ=255)
                                                                                                                                               atTCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCa  <  2:661435/151‑1 (MQ=255)
                                                                                                                                               atTCCCCAGGGTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCATGAGAATc   >  2:835576/1‑150 (MQ=255)
                                                                                                                                                ttCCCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATc   >  2:57307/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAACAGGCGAGCAGCGTTTTTACCGCGTAGCGCATATAGTCTGGATTGGTAAAAGCATCGGCGGCCATTGATGGCGGTTTGGCTGCGGGCGTTGGCGGCGTATTCGGGTCCATCTGACCCAGTTGTAACAACGTCTGGCAGATATTCTCCAGGTTACATTCCCGTGCCGCCATCGCTTCACTTTCACTGATCCGCCAGTCGCTTTGCCAGCACTGACCTTCAGCAACGGCATGCTGCAGCTTGTTGATTTCTGAAGCTAATTTTTGTCGGAATTCAATAATTGCCTGAGAATCA  >  NC_000913/4301994‑4302287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: