Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,564,425 G→A P89S (CCA→TCA)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,564,4250GA99.1% 416.6 / NA 108P89S (CCA→TCA) yjiLputative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (53/53);  total (53/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAA  >  NC_000913/4564277‑4564571
                                                                                                                                                    |                                                                                                                                                  
ccAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGacg                                                                                                                                                  <  4:499622/151‑1 (MQ=255)
ccAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGacg                                                                                                                                                  <  2:892516/151‑1 (MQ=255)
  aaGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGacgc                                                                                                                                                 >  2:1457911/1‑150 (MQ=255)
    gCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAg                                                                                                                                              <  2:56212/151‑1 (MQ=255)
        gcgcGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAAc                                                                                                                                          <  2:457910/151‑1 (MQ=255)
         cgcgAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAAcc                                                                                                                                         >  2:967511/1‑151 (MQ=255)
          gcgAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCg                                                                                                                                        >  1:1732453/1‑151 (MQ=255)
           cgAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGt                                                                                                                                       >  1:1327916/1‑151 (MQ=255)
            gagaTCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTg                                                                                                                                      >  3:508242/1‑151 (MQ=255)
              gaTCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCGAGAAACCGTgcg                                                                                                                                    <  2:1728418/151‑1 (MQ=255)
                tCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGt                                                                                                                                       <  1:1433545/146‑1 (MQ=255)
                  aCCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGccc                                                                                                                                 >  4:533989/1‑150 (MQ=255)
                        aGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGt                                                                                                                          >  3:77908/1‑151 (MQ=255)
                          gAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGa                                                                                                                        <  1:644204/151‑1 (MQ=255)
                               gCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAgg                                                                                                                    >  2:1682569/1‑150 (MQ=255)
                                    gTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGAttt                                                                                                              <  3:420773/151‑1 (MQ=255)
                                      gCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTcc                                                                                                            >  2:1423749/1‑151 (MQ=255)
                                      gCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTcc                                                                                                            <  1:780758/151‑1 (MQ=255)
                                      gCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTc                                                                                                             >  1:1164948/1‑150 (MQ=255)
                                      gCCCGCATCGCATTTATCATTCATCAGGAAATCGCGTAGATCACCTTCAGCATCAAGCGGAAACCCTTTGCTATCCGGACGCCCGATGTCGATTCCCGCGCGCGTTGCTGTCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGttt                                                                                                               <  1:543474/148‑1 (MQ=255)
                                       cccGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTcc                                                                                                            <  1:484232/150‑1 (MQ=255)
                                        ccgccgCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGt                                                                                                          >  2:960688/1‑151 (MQ=255)
                                             gcgcATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCt                                                                                                     <  2:826602/151‑1 (MQ=255)
                                              cgcATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTg                                                                                                    <  3:369373/151‑1 (MQ=255)
                                                cATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGtt                                                                                                  >  2:123457/1‑151 (MQ=255)
                                                   ttGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTa                                                                                                <  2:781188/150‑1 (MQ=255)
                                                   ttGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTAt                                                                                               >  1:330275/1‑151 (MQ=255)
                                                   ttGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTAt                                                                                               >  2:1667122/1‑151 (MQ=255)
                                                    tGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATc                                                                                              >  4:579719/1‑151 (MQ=255)
                                                     gTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATc                                                                                              <  2:24956/150‑1 (MQ=255)
                                                      tcattcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCgg                                                                                            >  2:1707973/1‑151 (MQ=255)
                                                       cattcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCa                                                                                                                                <  4:281049/114‑1 (MQ=255)
                                                       cattcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCa                                                                                                                                >  3:281067/1‑114 (MQ=255)
                                                       cattcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGc                                                                                           <  3:364566/151‑1 (MQ=255)
                                                        attcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGc                                                                                           >  3:322821/1‑150 (MQ=255)
                                                        attcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCa                                                                                          <  2:1062005/151‑1 (MQ=255)
                                                         ttcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCa                                                                                          >  2:1035365/1‑150 (MQ=255)
                                                           catcaGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCaaa                                                                                        <  2:460256/150‑1 (MQ=255)
                                                             tcaGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                                                                     >  3:257547/1‑151 (MQ=255)
                                                              caGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                                                                     <  2:862360/150‑1 (MQ=255)
                                                              caGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                                                                     <  4:287088/150‑1 (MQ=255)
                                                              caGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                                                                     <  1:187324/150‑1 (MQ=255)
                                                               aGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcca                                                                                   <  2:992542/151‑1 (MQ=255)
                                                                ggAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccac                                                                                  >  2:516587/1‑151 (MQ=255)
                                                                ggAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccac                                                                                  >  1:965943/1‑151 (MQ=255)
                                                                ggAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACGTTGCTGTCCTGACCACCGATGTCGATTACCGCGCTCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcca                                                                                   <  2:602223/150‑1 (MQ=255)
                                                                 gAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccaca                                                                                 <  1:217015/151‑2 (MQ=255)
                                                                  aaaTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                                                                 >  1:608642/1‑150 (MQ=255)
                                                                  aaaTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                                                                 >  1:475797/1‑150 (MQ=255)
                                                                  aaaTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                                                                 >  1:622533/1‑150 (MQ=255)
                                                                  aaaTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                                                                 >  3:257938/1‑150 (MQ=255)
                                                                     tCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGtt                                                                             >  2:1226631/1‑151 (MQ=255)
                                                                       gCACACGGTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGTCCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGAGCGCCCAGCCCGGGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAg                                                                               >  1:551443/1‑147 (MQ=255)
                                                                           aGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgccgcc                                                                       <  1:1734758/151‑1 (MQ=255)
                                                                            ggTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGccc                                                                      <  1:81959/151‑1 (MQ=255)
                                                                               tACCGTCATCATCAAGCTGAATCGCTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTGCCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGt                                                                    <  2:436403/150‑1 (MQ=255)
                                                                               tACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGt                                                                    <  2:1428356/150‑1 (MQ=255)
                                                                                aCCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTa                                                                   <  1:1582317/150‑1 (MQ=255)
                                                                                 cggTCATCAGCAAGCGGAATCACTTTGCTGGCCTGCCCCCCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCGAGCCCGTGACAGGAGATTTCCGTTGCCTGTTTATCGGCAAAATCCAGCAGTTGCCGCCCGTa                                                                   <  1:898278/147‑1 (MQ=255)
                                                                                    tcatcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTcc                                                                                                            <  3:484160/105‑1 (MQ=255)
                                                                                    tcatcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTcc                                                                                                            >  4:484142/1‑105 (MQ=255)
                                                                                     catcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGt                                                             >  1:723720/1‑151 (MQ=255)
                                                                                       tcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTg                                                            <  2:1591761/150‑1 (MQ=255)
                                                                                            aaGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTc                                                      >  2:1276035/1‑151 (MQ=255)
                                                                                             aGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCTGGTGAGCGTc                                                      >  1:378123/1‑150 (MQ=255)
                                                                                             aGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTc                                                      >  2:1691707/1‑150 (MQ=255)
                                                                                              gCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAg                                                    <  1:1012493/151‑1 (MQ=255)
                                                                                               cTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTc                                                      >  1:1044960/1‑148 (MQ=255)
                                                                                                     cACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGc                                             <  1:914231/151‑1 (MQ=255)
                                                                                                        tttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                          >  4:408134/1‑151 (MQ=255)
                                                                                                        tttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                          >  3:285492/1‑151 (MQ=255)
                                                                                                         ttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGt                                           <  1:315836/149‑1 (MQ=255)
                                                                                                         ttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTg                                         >  1:112999/1‑151 (MQ=255)
                                                                                                          tGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTg                                         <  1:491568/150‑1 (MQ=255)
                                                                                                           gCTGTACTGAACACCGAAGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGAGTCAGAAACGGCGTTg                                         >  1:1476253/1‑149 (MQ=255)
                                                                                                             tGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGtctc                                     >  3:383560/1‑151 (MQ=255)
                                                                                                              gTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGtct                                      <  2:1647834/149‑1 (MQ=255)
                                                                                                                ccTGACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTaa                                  <  2:752809/151‑1 (MQ=255)
                                                                                                                   gACCACCGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAAccc                               >  3:447528/1‑151 (MQ=255)
                                                                                                                    accaccGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCt                              <  2:1706057/151‑1 (MQ=255)
                                                                                                                     ccaccGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAGCCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCtt                             <  2:29173/151‑1 (MQ=255)
                                                                                                                       accGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcg                           <  2:391121/151‑1 (MQ=255)
                                                                                                                        ccGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcgc                          <  4:465819/151‑1 (MQ=255)
                                                                                                                         cGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcgcg                         >  4:11242/1‑151 (MQ=255)
                                                                                                                         cGATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcgcg                         <  3:62832/151‑1 (MQ=255)
                                                                                                                          gATGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCgcgc                        >  1:1077728/1‑151 (MQ=255)
                                                                                                                            tGTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCgcgc                        >  1:763709/1‑149 (MQ=255)
                                                                                                                             gTCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCaga                     <  2:723828/151‑1 (MQ=255)
                                                                                                                              tCGATTACCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCag                      >  2:866725/1‑149 (MQ=255)
                                                                                                                                    aCCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTccc              <  2:1629965/151‑1 (MQ=255)
                                                                                                                                    aCCGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTccc              >  2:999447/1‑151 (MQ=255)
                                                                                                                                     ccGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTccaa             <  1:284528/151‑3 (MQ=255)
                                                                                                                                     ccGCGCGCGTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCa             <  1:598166/151‑1 (MQ=255)
                                                                                                                                        cgcgcgTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGc          >  2:556108/1‑151 (MQ=255)
                                                                                                                                         gcgcgTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCt         >  2:1465651/1‑151 (MQ=255)
                                                                                                                                         gcgcgTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCt         <  2:1328063/151‑1 (MQ=255)
                                                                                                                                            cgTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTc       <  1:1423593/150‑1 (MQ=255)
                                                                                                                                            cgTTGCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTc       >  2:792282/1‑150 (MQ=255)
                                                                                                                                                gCTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTa   <  1:990768/150‑1 (MQ=255)
                                                                                                                                                 cTGACGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTaa  >  1:318598/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAA  >  NC_000913/4564277‑4564571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑