| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,480 | A→G | intergenic (+651/+85) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,480 | 0 | A | G | 86.4% | 93.6 / 6.0 | 46 | intergenic (+651/+85) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base G (15/23); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
GTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCT > NC_000913/1097331‑1097627 | gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGaa < 2:1215460/151‑1 (MQ=11) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGG‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCg < 1:1645472/151‑1 (MQ=9) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCg < 2:845484/151‑1 (MQ=14) tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACGCCCCGCGCTCTCCCCTCGGGCAGCCCCCCCCCCGCGTTCCCGGTCCTTTCAACCGGCTGCGGCCCGGT‑GGCCAAccccc > 1:1418811/1‑148 (MQ=2) ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTTGACTCGCCTCGCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTTCCGGTCTGTCCAACTGGGTGTCGCCCGTT‑GTCGGACCCCgg > 2:158656/1‑150 (MQ=11) taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTTGACTCGCCTTCCCCCCCCCGCGGGCAGCCCCCCCACCGCGTTCAAGGGCTGGTCAACCCGCCGTTGCCCGTTGGTCGAACCCCg > 2:467607/1‑149 (MQ=2) cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCg < 2:1634115/150‑1 (MQ=14) aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCAGGGGGGagtg > 1:1377048/1‑145 (MQ=0) ttCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAgcccc > 2:518346/1‑150 (MQ=9) tCACGGTCTGTCCCTCTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAc < 1:979126/145‑1 (MQ=16) cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAccccc > 1:197239/1‑148 (MQ=25) cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGAt < 2:80991/151‑1 (MQ=38) cACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGAt < 1:204791/151‑1 (MQ=38) cACGGTCTGCCCAACTGTCTGTTGTCGCTT‑ATCCACCTTCCCTCCGCGCCTCTCTTCCGCTCTGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAcc < 4:86920/145‑1 (MQ=2) cGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAc < 2:39555/151‑1 (MQ=255) ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTTGAAacccc > 1:1528029/1‑145 (MQ=255) ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGCGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATa < 1:530546/149‑1 (MQ=255) ggTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCGCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTCGGGGGGGGGtttaccctgattcctc > 4:304603/1‑136 (MQ=1) tGTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCCTACGtt > 2:1194954/1‑149 (MQ=255) gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAcccccctttcccgccc > 2:351983/1‑140 (MQ=1) gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCGCTaag > 2:1192603/1‑144 (MQ=11) gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGtt < 2:1377198/148‑1 (MQ=255) gTCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTg < 3:277155/149‑1 (MQ=255) gTCCAACTGGCTGGCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTGCAACCCCCATaacgt > 4:208496/1‑144 (MQ=11) tCCAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGATGGGGGGGTTCGAAcccccccca > 1:1596069/1‑139 (MQ=255) cAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGta < 2:1562319/151‑1 (MQ=255) cAACTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTCGAAACCCCCCTACGTTGcc > 2:827537/1‑148 (MQ=11) aaCTGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGtat < 2:1475750/151‑1 (MQ=255) tGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAAACCCCCCTACCGTGCCGtata > 2:101023/1‑149 (MQ=11) tGGCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGGTCGAACCCCCGATAAGCTGCCgcaaa > 1:186518/1‑145 (MQ=14) ggCTGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca < 2:1165240/150‑1 (MQ=255) gCTGTCGCGGGTT‑TTCGAGCCCCGGTCGGGGCTTTTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTGCTTTCGTACGAGCTCTTCGTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATgca < 2:1006921/149‑1 (MQ=17) tGTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCGCTACgctcccctctaccc > 1:1195495/1‑135 (MQ=11) gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacacac < 2:1596533/149‑1 (MQ=255) gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACtt < 1:1533202/151‑1 (MQ=255) gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 2:483276/150‑1 (MQ=255) gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 2:788829/150‑1 (MQ=255) gTCGCCAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACt < 1:1384254/150‑1 (MQ=255) ccAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCa < 2:590190/151‑1 (MQ=255) cAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg < 2:1634368/151‑1 (MQ=255) cAGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAg < 4:189338/151‑1 (MQ=255) aGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTAACGGGGGGGGTCGGAACCCCCCCACGTTTTCGGATacacaaacttccgg > 2:814292/1‑142 (MQ=11) aGTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGATCGAAACCCCCATTACTTGCCGcattaacccctt > 2:1006292/1‑137 (MQ=11) gTT‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCa < 3:186142/148‑1 (MQ=255) tt‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGATCCCCCGATACGTTGCCGTATACACTCTTTCCAg < 1:1612569/148‑1 (MQ=255) t‑GTCGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATACGTTGCCGTATACACACTTTTCCGGCg > 4:351304/1‑150 (MQ=255) cGAGCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCt < 2:1486082/151‑1 (MQ=255) | GTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTT‑GTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCT > NC_000913/1097331‑1097627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |