Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,129,249 T→C L471L (TTG→CTG)  murJ → putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,2490TC98.9% 346.9 / NA 94L471L (TTG→CTG) murJputative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/61);  minor base A (1/0);  total (33/61)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395
                                                                                                                                                   |                                                                                                                                                      
gCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTgg                                                                                                                                                     >  2:554382/1‑151 (MQ=255)
  ttCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTggg                                                                                                                                                    >  1:1588612/1‑150 (MQ=255)
   tCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTggg                                                                                                                                                    <  4:525705/149‑1 (MQ=255)
    cGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTa                                                                                                                                                  <  1:394360/150‑1 (MQ=255)
    cGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTAc                                                                                                                                                 >  4:201408/1‑151 (MQ=255)
    cGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTAc                                                                                                                                                 >  2:704880/1‑151 (MQ=255)
        gctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCa                                                                                                                                               <  2:1713838/149‑1 (MQ=255)
        gctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATg                                                                                                                                             <  3:363959/151‑1 (MQ=255)
             aCTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGccc                                                                                                                                          >  2:1655669/1‑149 (MQ=255)
             aCTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCt                                                                                                                                         <  4:371317/150‑1 (MQ=255)
               tGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGc                                                                                                                                      >  2:1565393/1‑151 (MQ=255)
               tGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGGACCATGCCCTGGc                                                                                                                                      >  2:359896/1‑151 (MQ=255)
                 gCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGt                                                                                                                                    <  2:1483599/151‑1 (MQ=255)
                 gCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGt                                                                                                                                    >  2:392705/1‑151 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                  >  2:1192790/1‑151 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                  >  2:1718283/1‑151 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                  >  2:43915/1‑151 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                  <  2:539974/151‑1 (MQ=255)
                   aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                  <  1:1476235/151‑1 (MQ=255)
                     ttGCGTAAGTAGAAAATCTCTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGTTCGCGGCCATCGCGATGTCTTGCGAGCTTTTTGGTGTATTACATTCCATGCTGGCCGGATCACTGGGTACCATGCCCTggacctt                                                                                                                                  >  1:132074/1‑144 (MQ=255)
                      tGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGGTGTTTGGCGTGCTTTTATGTATTTTACATATCCTTCCGGAGTGGTCGCTGGGGACCATGCCCTGGCGGTTACt                                                                                                                               >  1:1008974/1‑151 (MQ=255)
                       gCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACt                                                                                                                               <  2:1033837/150‑1 (MQ=255)
                       gCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACt                                                                                                                               >  2:523664/1‑150 (MQ=255)
                       gCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTg                                                                                                                              <  3:395329/151‑1 (MQ=255)
                          tAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGt                                                                                                                           >  3:445020/1‑151 (MQ=255)
                           aaGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGtt                                                                                                                          <  1:1389776/151‑1 (MQ=255)
                             gCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGttt                                                                                                                         >  1:1504815/1‑150 (MQ=255)
                              cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGGATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGGTTa                                                                                                                        >  1:142580/1‑150 (MQ=255)
                               aGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAt                                                                                                                      >  1:1635198/1‑151 (MQ=255)
                                gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAt                                                                                                                      >  2:1493690/1‑150 (MQ=255)
                                gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                     <  1:1443236/151‑1 (MQ=255)
                                gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                     <  1:62114/151‑1 (MQ=255)
                                gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                     <  1:1524922/151‑1 (MQ=255)
                                 aaaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATgg                                                                                                                    <  2:1625873/151‑1 (MQ=255)
                                 aaaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                     <  1:1167617/150‑1 (MQ=255)
                                 aaaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                     <  1:601445/150‑1 (MQ=255)
                                   aaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCg                                                                                                                 <  1:913223/151‑1 (MQ=255)
                                      cTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGt                                                                                                               >  2:1611771/1‑150 (MQ=255)
                                        ttACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTc                                                                                                              <  2:872174/149‑1 (MQ=255)
                                          aCCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTg                                                                                                          <  1:811533/150‑1 (MQ=255)
                                           ccccGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTg                                                                                                          <  1:997207/149‑1 (MQ=255)
                                            cccGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCgg                                                                                                                >  1:155457/1‑143 (MQ=255)
                                            cccGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTg                                                                                                      <  1:1373807/151‑1 (MQ=255)
                                             ccGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCg                                                                                                            <  1:159994/145‑1 (MQ=255)
                                              cGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGTGTTTACTGCGTTTAATGGCGGTCGTGCTGGc                                                                                                    <  1:1346913/151‑1 (MQ=255)
                                                 aaCCCGGCTGGATGGCGTTTCCGATGCGTCTGGTGGTGGCGGTACTGGTGTTGGCTGGCGTGCTTTTCGGTACGTTACCTATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTACTGGCggcggcgcccgcg                                                                                                     >  1:1678596/1‑138 (MQ=255)
                                                  aCCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACCGGGGGTGTCTGGCGTGCTTTTAGGGATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGGCCTGGTGTTTACTGCGTTTAATGGCGGGAGTGGTGGAgggg                                                                                                >  1:334935/1‑151 (MQ=255)
                                                   cccGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGGTCGGGCgggggggg                                                                                                >  1:1233528/1‑151 (MQ=255)
                                                    ccGGCTGGATGGCTTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGGTTACTGCGTTTAATCGCGGTCATGCTCTCGCGGAt                                                                                              <  1:265848/151‑1 (MQ=255)
                                                    ccGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCgggtgtgtgggggggg                                                                                                  >  4:111276/1‑135 (MQ=255)
                                                         tGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATAATGCCGGAGTTGTCCCTGGGTACCATGCCCTGGCGTTTACTGCGTTTTATGGCGGTCGTGACGGGCggggg                                                                                               >  1:566856/1‑145 (MQ=255)
                                                          ggATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCcgc                                                                                        <  2:50089/151‑1 (MQ=255)
                                                          ggATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCcgc                                                                                        <  2:1072523/151‑1 (MQ=255)
                                                          ggATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCcg                                                                                         <  2:204408/150‑1 (MQ=255)
                                                            aTGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGt                                                                                      <  1:1655505/151‑1 (MQ=255)
                                                                 gTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTGGTGCTGGCGGGGATTGCCGCGTACtt                                                                                  <  1:393560/150‑1 (MQ=255)
                                                                 gTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGGGGCGGGGGGGGGTTGCcgcgg                                                                                      >  2:389731/1‑146 (MQ=255)
                                                                 gTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTc                                                                                 <  1:228240/151‑1 (MQ=255)
                                                                  tttCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGGTGggggggggtggcgccgcatt                                                                                  >  2:1183288/1‑135 (MQ=255)
                                                                    tCTGTTGCGTCTGGTGGAGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGGGGTAAATGGGTAGCATGCCCTGGCGTTTACTGCGTTTAATGGCGGGGGTGGGGGCggggggggg                                                                                           >  1:177676/1‑133 (MQ=255)
                                                                       gTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCa                                                                          <  1:471280/151‑1 (MQ=255)
                                                                          gCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCTCTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTg                                                                       <  2:1019278/151‑1 (MQ=255)
                                                                           cGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGGGGGGGGTGCCGCGGGCTTctcttcgccgg                                                                       >  2:78986/1‑144 (MQ=255)
                                                                                   ggtggCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACt                                                               <  4:403148/150‑1 (MQ=255)
                                                                                    gttgCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTTCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTCCTTCGCTGCACTGGCGGTACTgg                                                             <  1:1049046/148‑1 (MQ=255)
                                                                                    gtggCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTgg                                                             <  2:1113160/151‑1 (MQ=255)
                                                                                      ggCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGGTGGGGGGGGGTTTCGCGGACTTTTCTTCACcggcgggggcaggg                                                           >  2:1114161/1‑143 (MQ=255)
                                                                                       gCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCt                                                          <  2:983867/151‑1 (MQ=255)
                                                                                        cGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCtt                                                         <  1:1071004/151‑1 (MQ=255)
                                                                                            aCTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCaa                                                      <  1:592933/150‑1 (MQ=255)
                                                                                              tGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGGGTGGCCCGGACTTTTCTGCCCcggcgggggctggggttcaa                                                     >  3:595854/1‑135 (MQ=255)
                                                                                               ggTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGt                                                   <  4:417563/150‑1 (MQ=255)
                                                                                                gTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTa                                                 <  1:770698/151‑1 (MQ=255)
                                                                                                  ggtggctagagTGCTTTTAGGTGTGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGGGTTTACTGCGTTTAATGGCGATCGTGCTGGCGGGGATTGCCGCGTACATCGCTGCACAGGCGGTACTGGGCTTCAAAGtt                                                  <  1:679938/138‑1 (MQ=255)
                                                                                                         ggCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAAttt                                         <  2:1547487/150‑1 (MQ=255)
                                                                                                         ggCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAAtt                                          <  1:1503700/149‑1 (MQ=255)
                                                                                                          gCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGc                                       <  1:815810/151‑1 (MQ=255)
                                                                                                             tGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCGCTGGGTGCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGccc                                     <  1:378415/150‑1 (MQ=255)
                                                                                                               cTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgc                                   <  2:706300/150‑1 (MQ=255)
                                                                                                                 tttAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgcc                                  <  1:943957/149‑1 (MQ=255)
                                                                                                                  ttAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCgg                                <  1:654536/150‑1 (MQ=255)
                                                                                                                   tAGGTATGTTGCATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGa                               <  1:685742/150‑1 (MQ=255)
                                                                                                                      gTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGgt                           <  2:498124/151‑1 (MQ=255)
                                                                                                                        aTGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGGGGGGGGTGTCGCGGGCTTTCCTGCCCCGGGGGGGGCGGGGGTTCTAAATTAAAAAATTTTCCCCCCggcggggg                          >  2:910179/1‑145 (MQ=255)
                                                                                                                          gTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTa                        <  1:350393/150‑1 (MQ=255)
                                                                                                                            tACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAAc                      <  2:1009103/150‑1 (MQ=255)
                                                                                                                             aCATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACa                     <  3:309306/150‑1 (MQ=255)
                                                                                                                             aCATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACa                     <  4:107483/150‑1 (MQ=255)
                                                                                                                                       ccGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCgg          <  1:507648/151‑1 (MQ=255)
                                                                                                                                         ggAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGcc        <  1:549690/151‑1 (MQ=255)
                                                                                                                                         ggAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGcc        <  1:523583/151‑1 (MQ=255)
                                                                                                                                         ggAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGca        <  1:917176/151‑2 (MQ=255)
                                                                                                                                          gAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCt       <  2:818290/151‑1 (MQ=255)
                                                                                                                                               gTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt  <  1:1250426/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: