Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 218,942 A→G V218A (GTT→GCT)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913218,9420AG99.3% 508.7 / NA 140V218A (GTT→GCT) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (81/58);  minor base C (1/0);  total (82/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

GGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTT  >  NC_000913/218794‑219086
                                                                                                                                                    |                                                                                                                                                
ggAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCagcgagcg                                                                                                                                                <  2:932609/151‑1 (MQ=255)
 gAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGt                                                                                                                                               <  1:970529/151‑1 (MQ=255)
  aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGtt                                                                                                                                              >  1:1025360/1‑151 (MQ=255)
  aaGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGtt                                                                                                                                              >  2:1403247/1‑151 (MQ=255)
     gTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTaaa                                                                                                                                           <  3:467165/151‑1 (MQ=255)
        gAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATc                                                                                                                                        >  2:736/1‑151 (MQ=255)
        gAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATc                                                                                                                                        >  1:814528/1‑151 (MQ=255)
         aaCAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCa                                                                                                                                       <  4:411626/151‑1 (MQ=255)
         aaCAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCa                                                                                                                                       <  1:614890/151‑1 (MQ=255)
           cAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATg                                                                                                                                     <  2:1223826/151‑1 (MQ=255)
            aGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGt                                                                                                                                    >  2:648689/1‑151 (MQ=255)
                  aaGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGcc                                                                                                                                >  1:1612623/1‑149 (MQ=255)
                   aGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGcc                                                                                                                                >  2:583073/1‑148 (MQ=255)
                      cGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACgg                                                                                                                          >  1:992059/1‑151 (MQ=255)
                       gAGCCTGGGACAAAAAAAAGTGATTTAGGGTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACgg                                                                                                                          <  1:742888/150‑1 (MQ=255)
                         gCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCa                                                                                                                        <  1:906489/150‑1 (MQ=255)
                           cTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATc                                                                                                                                        <  3:79192/132‑1 (MQ=255)
                           cTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATc                                                                                                                                        >  4:79188/1‑132 (MQ=255)
                           cTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCAt                                                                                                                       >  3:22074/1‑149 (MQ=255)
                              ggACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtt                                                                                                                  <  1:1592488/151‑1 (MQ=255)
                               gACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGttt                                                                                                                 <  2:180550/151‑1 (MQ=255)
                                aCAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtttt                                                                                                                >  2:1662502/1‑151 (MQ=255)
                                aCAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGtttt                                                                                                                >  4:213401/1‑151 (MQ=255)
                                 cAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTg                                                                                                               <  3:35717/151‑1 (MQ=255)
                                  aaaaaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGc                                                                                                              <  2:1022744/151‑1 (MQ=255)
                                     aaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCg                                                                                                           >  2:1658912/1‑151 (MQ=255)
                                     aaaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCg                                                                                                           >  2:760845/1‑151 (MQ=255)
                                      aaaaGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGt                                                                                                          >  1:1524968/1‑151 (MQ=255)
                                            gATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCt                                                                                                     <  2:1607701/150‑1 (MQ=255)
                                            gATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                    >  2:328230/1‑151 (MQ=255)
                                             aTTTAGCTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAATACTTCAAAACCTGCGTCGGGGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc                                                                                                    >  1:1137153/1‑150 (MQ=255)
                                                 aGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                                                                                               <  4:173957/151‑1 (MQ=255)
                                                 aGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCtt                                                                                               <  2:829211/151‑1 (MQ=255)
                                                   tttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCttt                                                                                              >  1:1529483/1‑150 (MQ=255)
                                                    ttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTa                                                                                             <  3:394398/150‑1 (MQ=255)
                                                    ttACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc                                                                                            >  1:41357/1‑151 (MQ=255)
                                                     tACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACg                                                                                           >  1:1385904/1‑151 (MQ=255)
                                                      aCCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACg                                                                                           >  2:82867/1‑150 (MQ=255)
                                                        cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                         >  2:1078848/1‑150 (MQ=255)
                                                        cAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa                                                                                        <  2:410759/151‑1 (MQ=255)
                                                         aGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGAt                                                                                         >  3:503598/1‑149 (MQ=255)
                                                             cAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCg                                                                                   >  4:235830/1‑151 (MQ=255)
                                                              aaaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCgg                                                                                  >  4:399623/1‑151 (MQ=255)
                                                               aaaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCggg                                                                                 >  1:877618/1‑151 (MQ=255)
                                                                aaGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgc                                                                                <  1:768236/151‑1 (MQ=255)
                                                                 aGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgc                                                                                >  4:36734/1‑150 (MQ=255)
                                                                  gAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGgcgc                                                                              >  2:256519/1‑151 (MQ=255)
                                                                   aaaTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg                                                                             <  3:343618/151‑1 (MQ=255)
                                                                      tGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                          >  1:75186/1‑151 (MQ=255)
                                                                      tGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                          >  2:163945/1‑151 (MQ=255)
                                                                              gagaAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGcc                                                                  <  3:438035/151‑1 (MQ=255)
                                                                               agaAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCa                                                                 <  1:960374/151‑1 (MQ=255)
                                                                                 aaGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGt                                                               >  4:522130/1‑151 (MQ=255)
                                                                                    ggCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACt                                                            >  2:1469871/1‑151 (MQ=255)
                                                                                      cGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTc                                                          <  1:1457048/151‑1 (MQ=255)
                                                                                      cGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTc                                                          >  2:595194/1‑151 (MQ=255)
                                                                                       gTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcg                                                         <  2:872113/151‑1 (MQ=255)
                                                                                             ttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGAt                                                    <  1:582803/150‑1 (MQ=255)
                                                                                              tAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATa                                                   >  3:267973/1‑150 (MQ=255)
                                                                                               aaCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATaa                                                  <  2:320594/150‑1 (MQ=255)
                                                                                                aCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAAc                                                >  3:579043/1‑151 (MQ=255)
                                                                                                 cgcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACa                                               <  2:463054/151‑1 (MQ=255)
                                                                                                   cgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGc                                             <  2:992185/151‑1 (MQ=255)
                                                                                                    ggTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGGCAGTACTTCGCGGATAAACAGCg                                            >  1:264972/1‑151 (MQ=255)
                                                                                                     gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGt                                           <  2:1542989/151‑1 (MQ=255)
                                                                                                     gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGt                                           >  2:930658/1‑151 (MQ=255)
                                                                                                     gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGt                                           >  2:991315/1‑151 (MQ=255)
                                                                                                      ttCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGtt                                          >  2:775640/1‑151 (MQ=255)
                                                                                                       tCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGtt                                          <  3:209779/150‑1 (MQ=255)
                                                                                                        ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTa                                         >  2:1639913/1‑150 (MQ=255)
                                                                                                         cAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAAc                                       >  3:160005/1‑151 (MQ=255)
                                                                                                         cAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAAc                                       >  3:413862/1‑151 (MQ=255)
                                                                                                          aGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACt                                      >  4:582990/1‑151 (MQ=255)
                                                                                                           gcgcAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACt                                      >  2:1352624/1‑150 (MQ=255)
                                                                                                                aaGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGAt                                <  4:22074/151‑1 (MQ=255)
                                                                                                                aaGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGAt                                >  2:1409726/1‑151 (MQ=255)
                                                                                                                aaGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGAt                                <  1:778658/151‑1 (MQ=255)
                                                                                                                 aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATa                               <  1:1580346/151‑1 (MQ=255)
                                                                                                                  gACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATa                               >  1:1264822/1‑150 (MQ=255)
                                                                                                                  gACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATa                               >  2:262971/1‑150 (MQ=255)
                                                                                                                    cTTCACAACCTGCGTCGGTGTCGCGGCAGCTAGCGTTAAATTCATGTAGCCAGACCGCATAGGCTTTGCCCGTTTCCTCCCCGTTTCGATAGGCCGGCCGCGCTTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATaa                              <  1:1045630/149‑1 (MQ=255)
                                                                                                                    cTTCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAAc                             >  1:1213230/1‑150 (MQ=255)
                                                                                                                     ttCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                           >  2:337873/1‑151 (MQ=255)
                                                                                                                     ttCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                           >  4:165724/1‑151 (MQ=255)
                                                                                                                      tCAAAACCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                           <  4:249586/150‑1 (MQ=255)
                                                                                                                           aCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTcc                     >  4:574615/1‑151 (MQ=255)
                                                                                                                           aCCTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTcc                     <  2:988528/151‑1 (MQ=255)
                                                                                                                            ccTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTcc                     >  3:458353/1‑150 (MQ=255)
                                                                                                                            ccTGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCa                    <  2:525520/151‑1 (MQ=255)
                                                                                                                              tGCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGc                  >  1:127702/1‑151 (MQ=255)
                                                                                                                               gCGTCGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAg                   <  2:1025489/149‑1 (MQ=255)
                                                                                                                                   cGGTGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCa              >  2:174392/1‑150 (MQ=255)
                                                                                                                                      tGACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCaaa            >  1:460463/1‑149 (MQ=255)
                                                                                                                                       gACGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgc         >  4:113274/1‑151 (MQ=255)
                                                                                                                                        aCGCGCCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgct        <  1:437857/151‑1 (MQ=255)
                                                                                                                                            gcCAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtt    >  1:1206599/1‑151 (MQ=255)
                                                                                                                                             ccAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCttt   >  3:170601/1‑151 (MQ=255)
                                                                                                                                              cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCGCCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtt    <  1:1223399/149‑1 (MQ=255)
                                                                                                                                              cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtttt  <  1:163917/151‑1 (MQ=255)
                                                                                                                                              cAGCGAGCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtttt  >  3:491265/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTT  >  NC_000913/218794‑219086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑