Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,154,782 A→G E224G (GAA→GGA)  yceG → septation protein, ampicillin sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,154,7820AG99.3% 550.6 / NA 148E224G (GAA→GGA) yceGseptation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (72/75);  minor base T (1/0);  total (73/75)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 148 total aligned reads displayed.

TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924
                                                                                                                                                     |                                                                                                                                              
  gcgTTCCACAAGCGAGCGAACAAGAAATGGGTGAAAGGGGTCAATACCGCGTGGGAAGGGCGGGCGGAGGTTCTGTCTTATAAAGAAAAAAACCAGTTGGTGATGATGGCATAAATTAGCAAAAAAGAAACCGCCGTTGCCAGTGGAc                                                                                                                                              <  1:1170518/148‑1 (MQ=255)
     ttACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGAt                                                                                                                                         >  2:930321/1‑150 (MQ=255)
     ttACTCAAGCGAGCGCAAAAGAAAAGGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGa                                                                                                                                          <  3:449905/149‑1 (MQ=255)
        cTCAGGCGAGGGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGGGGACGGTCTGCTTTATAAAGATAAAAACCAGTGGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATaa                                                                                                                                       <  1:402050/149‑1 (MQ=255)
         tCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGt                                                                                                                                    >  4:268513/1‑151 (MQ=255)
          cAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGt                                                                                                                                    >  1:1267934/1‑151 (MQ=255)
           aaGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGAAAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAgg                                                                                                                                     <  2:1007204/148‑1 (MQ=255)
           aaGCGAGCGCACAAAAAAAGGGGAAAAGGGGTAAATAGCGCCTGGGAAGGGCGGGCGGACGGTCTGCCTTATAAAGATAAAAACCGGTGGGTGACGATGGAATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGtt                                                                                                                                   <  2:1702068/150‑1 (MQ=255)
           aaGCGAGCGCAAAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAgg                                                                                                                                     <  2:1541797/148‑1 (MQ=255)
            agcgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGc                                                                                                                                 >  3:59686/1‑151 (MQ=255)
             gcgagcgCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGGGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGGGGACGCGATAAGGTTGcc                                                                                                                                >  2:1280591/1‑151 (MQ=255)
               gagcgCACAAGAAAATGGTGAAAGCGGTGGATAGCGCCTGGGAAGGGAGAGCGGACAGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGTACGCGATAAGGTTGCCTc                                                                                                                              >  1:1479926/1‑151 (MQ=255)
                 gcgcACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTc                                                                                                                              >  1:1625016/1‑149 (MQ=255)
                     ccaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAtt                                                                                                                        >  1:1403085/2‑151 (MQ=255)
                      caAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAttt                                                                                                                       >  1:639875/1‑151 (MQ=255)
                      caAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAttt                                                                                                                       >  2:817700/1‑151 (MQ=255)
                           aaaTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCa                                                                                                                   >  3:513173/1‑150 (MQ=255)
                            aaTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTGGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAAc                                                                                                                 <  2:767131/151‑1 (MQ=255)
                              tGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCg                                                                                                               >  4:554230/1‑151 (MQ=255)
                                gTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGtt                                                                                                             >  2:167580/1‑151 (MQ=255)
                                   aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTAAAAAGAAAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTa                                                                                                           <  4:459571/150‑1 (MQ=255)
                                        gggcGAAAGCCCCTGGGAAGGCCGGGGGGACGGTCTGCCTTATAAAGAAAAAAACCATTTGGTGACGGTGGCACCAATTATCAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCa                                                                                                       <  2:1403239/146‑1 (MQ=255)
                                            gATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTgg                                                                                                   <  2:421690/149‑1 (MQ=255)
                                             atggcgcCGGGGAAGGGCGTGCGAAGGGTCTGCCTTAAAAAGAAAAAACCCAGTTGGGGACGATGGTTTAAATTATGGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCAt                                                                                                      <  1:594578/142‑1 (MQ=255)
                                                 cgcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATgcgc                                                                                            >  2:1228012/1‑151 (MQ=255)
                                                  gcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGcc                                                                                           >  2:1229170/1‑151 (MQ=255)
                                                   ccTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCt                                                                                          >  1:1147858/1‑151 (MQ=255)
                                                    cTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         >  1:1656674/1‑151 (MQ=255)
                                                     tGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGc                                                                                        >  2:1604297/1‑151 (MQ=255)
                                                      gggAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                         <  2:1311058/149‑1 (MQ=255)
                                                       ggaggggttggCGGACGTGCAGCTTTATAAAGATAAAAAGCGGTTTGTGGCGATGGCCTTAATCTCCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAg                                                                                      <  1:1091640/141‑1 (MQ=255)
                                                       gaaaGGGCGTGCGGACGTTCTGCCTTATAAAGAAAAAACCCAGTTGGTGACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAg                                                                                      <  2:245253/149‑1 (MQ=255)
                                                           gggcgggcGGACGGTCTGCCTTAAAAAGAAAAAACCCAGTTGGTGACGATGGAATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  <  1:104133/151‑1 (MQ=255)
                                                           gggCGTGCGGACGGTCTGCCTTAAAAAGATAAAACCCAGTTGGTGACGATGGCGTCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccg                                                                                  <  2:1436946/151‑1 (MQ=255)
                                                             gCGTGCGGACGGTCTGCCTTAAAAAGATAAAAACCATTTGGTGACGAGGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgac                                                                                <  2:316694/151‑1 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  1:1170716/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  1:391279/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                             >  2:346968/1‑151 (MQ=255)
                                                                tGCGGACGGTCTGCCTTAAAAAGATAAAAACCAGTTGTTGACGAAGGCATCATTTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAgaccgacc                                                                               <  4:539600/149‑1 (MQ=255)
                                                                   ggACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          >  3:226701/1‑151 (MQ=255)
                                                                   ggACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          >  1:1660712/1‑151 (MQ=255)
                                                                     aCGGTCTGCCTTATAAAGATAAAAACCATGGGGTGACAATCGATTTAATTTTCAAAAAAAAATCCGCCGTTGACAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACcgacccgacc                                                                          <  1:462925/149‑1 (MQ=255)
                                                                       ggTCTGCCTTATAAAGATAAAAACCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGa                                                                      <  2:569059/151‑1 (MQ=255)
                                                                            gCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 >  2:1724154/1‑151 (MQ=255)
                                                                             ccTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACg                                                                >  1:808228/1‑151 (MQ=255)
                                                                             ccTTAAAAAAAAAAAATCCAGTGGGGGACGATGGCATCAATTACGGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 <  1:175673/150‑1 (MQ=255)
                                                                              cTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                               >  2:1006699/1‑151 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTTGGTGAGGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTAc                                                                 <  3:130434/148‑1 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACggg                                                              >  2:935377/1‑151 (MQ=255)
                                                                               ttATAAAGATAAAAACCAGTGGGTGACGTTGGCATCATTTATCGAAAAATAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                               <  1:1677166/150‑1 (MQ=255)
                                                                                 ataAAGATAAAACCAAGTAGGTGAGAATGGCATCAATTACCAAAAAACACACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTACTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGGCCCGACCGTGATTTACGGGAt                                                            <  1:799821/151‑1 (MQ=255)
                                                                                   aaaaATAAAAACCAGTTGTTGACAATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  1:514449/150‑1 (MQ=255)
                                                                                   aaaGATAAAACCCAGTTGGTGAGGATGGCATCAATTATCGAAAAAAAATCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  2:12831/150‑1 (MQ=255)
                                                                                   aaaGATAAAACCCAGTTGGTGACGAGGGCGTAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGa                                                             <  4:220751/148‑1 (MQ=255)
                                                                                   aaaGAAAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATg                                                           <  1:44996/150‑1 (MQ=255)
                                                                                    aaGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                         >  1:421567/1‑151 (MQ=255)
                                                                                     aGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                         >  4:229991/1‑150 (MQ=255)
                                                                                        aaaaaaCCAATTGGGGGAGGAGGGCATCAATTATGAAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGgaga                                                      <  1:1462059/150‑1 (MQ=255)
                                                                                         aaaaaCCAGTGGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                    <  4:421473/151‑1 (MQ=255)
                                                                                              ccAGTTGGTGACGATGCCATAAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTtat                                               <  2:1350607/151‑1 (MQ=255)
                                                                                              ccAGTTGGTGACGAGGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTta                                                <  3:595106/150‑1 (MQ=255)
                                                                                                       tGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCACCCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCa                                        >  1:340460/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                       >  2:652684/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaa                                       >  1:444195/1‑150 (MQ=255)
                                                                                                        gACGATGGCATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCaaa                                      <  2:274998/151‑1 (MQ=255)
                                                                                                        gACGATGGCATCAATTACCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCa                                        <  1:1643441/149‑1 (MQ=255)
                                                                                                          cGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    >  1:1279427/1‑151 (MQ=255)
                                                                                                           gagggCAACAATTAACAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAAc                                     <  1:1441068/146‑1 (MQ=255)
                                                                                                           gATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACtt                                   >  2:602903/1‑151 (MQ=255)
                                                                                                            aTGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACt                                    <  3:483727/149‑1 (MQ=255)
                                                                                                              ggCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                  <  2:927111/149‑1 (MQ=255)
                                                                                                              ggCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtct                                <  2:261712/151‑1 (MQ=255)
                                                                                                                cATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  1:991786/1‑151 (MQ=255)
                                                                                                                cATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  2:330406/1‑151 (MQ=255)
                                                                                                                 aTCATTTATTGATAAAGAAACCGCCGTTGCCAGTGGGCGCGCAATGGTTTCCTCAGCATTAATCAACCGTTTACGCATTGGTAGGCGCGTGGAGAACGACCCGACCGTGATTTACGGGAAGGGAGAGCGTTATAATGGCCAACTTtc                                 >  1:1005727/1‑147 (MQ=255)
                                                                                                                 aTCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCg                              >  2:997044/1‑150 (MQ=255)
                                                                                                                 aTCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  2:1079850/1‑151 (MQ=255)
                                                                                                                 aTCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  1:324855/1‑151 (MQ=255)
                                                                                                                 aTCAATTATCGAAAAAAAACCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             <  2:828626/151‑1 (MQ=255)
                                                                                                                  tCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             >  1:685411/1‑150 (MQ=255)
                                                                                                                  tCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGt                             <  1:1336250/150‑1 (MQ=255)
                                                                                                                      ttATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGc                           >  1:1527076/1‑148 (MQ=255)
                                                                                                                      ttACCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAg                         <  4:282160/150‑1 (MQ=255)
                                                                                                                        aTCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                       <  2:655803/150‑1 (MQ=255)
                                                                                                                           gAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                   >  1:681245/1‑151 (MQ=255)
                                                                                                                           gAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                   >  3:444215/1‑151 (MQ=255)
                                                                                                                             aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGAc                       <  2:776018/146‑1 (MQ=255)
                                                                                                                             aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                    <  1:698682/149‑1 (MQ=255)
                                                                                                                              aaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGaa                 <  3:422544/151‑1 (MQ=255)
                                                                                                                              aaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGa                  >  2:1375734/1‑150 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            >  1:1283403/1‑151 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            >  2:434866/1‑151 (MQ=255)
                                                                                                                                   aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc            <  2:114160/151‑1 (MQ=255)
                                                                                                                                        gccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGAc         >  1:1566902/1‑149 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      >  2:1525477/1‑151 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      >  2:1434083/1‑151 (MQ=255)
                                                                                                                                         ccgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGc      <  1:1059886/151‑1 (MQ=255)
                                                                                                                                          cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg     >  1:786045/1‑151 (MQ=255)
                                                                                                                                          cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg     >  2:112740/1‑151 (MQ=255)
                                                                                                                                            ttGCCAGTGGACGCGGTAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGt    >  2:1334472/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
TCGCGTTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTAT  >  NC_000913/1154635‑1154924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: