BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,015	T→C	A454A (GCA→GCG)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,015	0	T	C	100.0%	414.4 / NA	115	A454A (GCA→GCG)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (63/52); total (63/52)

Only 100 of 118 total aligned reads displayed.

CGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAG  >  NC_000913/1245867‑1246161

cGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGgcgc                                                                                                                                                  >  2:275640/1‑151 (MQ=255)

cGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGgcgc                                                                                                                                                  <  2:146788/151‑1 (MQ=255)

 ggtggtGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGcc                                                                                                                                                 >  2:615966/1‑151 (MQ=255)

 ggtggtGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGcc                                                                                                                                                 >  3:523327/1‑151 (MQ=255)

 ggtggtGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGcc                                                                                                                                                 >  1:220750/1‑151 (MQ=255)

     gtgCTTACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCa                                                                                                                                               <  1:1062018/149‑1 (MQ=255)

       gCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGcc                                                                                                                                            >  1:483814/1‑150 (MQ=255)

        cTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCAt                                                                                                                                          >  4:499348/1‑151 (MQ=255)

         tGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCAtt                                                                                                                                         >  1:1021431/1‑151 (MQ=255)

          gACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGTCAttt                                                                                                                                        <  1:741210/151‑1 (MQ=255)

          gACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCAttt                                                                                                                                        <  3:143237/151‑1 (MQ=255)

           aCATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTg                                                                                                                                       <  2:69195/151‑1 (MQ=255)

            catcatATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTgg                                                                                                                                      >  1:1456073/1‑151 (MQ=255)

              tcatATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGgcg                                                                                                                                    >  2:170246/1‑151 (MQ=255)

                    ttttCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATccc                                                                                                                              >  4:295400/1‑151 (MQ=255)

                      ttCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCa                                                                                                                             <  1:661020/150‑1 (MQ=255)

                       tCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAtt                                                                                                                           >  1:1557867/1‑151 (MQ=255)

                       tCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAt                                                                                                                            <  3:87279/150‑1 (MQ=255)

                         caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAttgt                                                                                                                         >  3:565082/1‑151 (MQ=255)

                         caccaGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAttgt                                                                                                                         <  1:1062497/151‑1 (MQ=255)

                            caGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGc                                                                                                                      >  3:526269/1‑151 (MQ=255)

                            caGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGc                                                                                                                      <  4:421269/151‑1 (MQ=255)

                             aGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGcc                                                                                                                     >  1:996018/1‑151 (MQ=255)

                                tttttCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCAc                                                                                                                  >  2:679009/1‑151 (MQ=255)

                                 ttttCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACt                                                                                                                 >  2:1254848/1‑151 (MQ=255)

                                       cccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACtttt                                                                                                              <  2:1269751/148‑1 (MQ=255)

                                       cccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACtttt                                                                                                              <  2:1187567/148‑1 (MQ=255)

                                       cccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACtttt                                                                                                              <  2:555214/148‑1 (MQ=255)

                                       cccGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCAc                                                                                                           >  1:433781/1‑151 (MQ=255)

                                         cGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCg                                                                                                         >  2:831202/1‑151 (MQ=255)

                                                aGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACgtggt                                                                                                  >  2:922039/1‑151 (MQ=255)

                                                 ggTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCAGTGTTGCCCACTTTTCACCGACgtggtg                                                                                                 <  4:193265/151‑1 (MQ=255)

                                                     tgCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTCTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCAT‑CCATTGTTGCCCACTTTTCACCGACGTGGTGtt                                                                                               >  3:579057/1‑148 (MQ=255)

                                                      gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTc                                                                                              >  2:710376/1‑149 (MQ=255)

                                                      gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCa                                                                                             <  3:455258/150‑1 (MQ=255)

                                                      gCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAg                                                                                            <  2:556331/151‑1 (MQ=255)

                                                       cTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAgg                                                                                           >  1:113651/1‑151 (MQ=255)

                                                        tGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCTGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgc                                                                                          >  3:361770/1‑151 (MQ=255)

                                                          aaCATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgccg                                                                                        <  2:1078416/151‑1 (MQ=255)

                                                          aaCATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgccg                                                                                        >  2:1535763/1‑151 (MQ=255)

                                                            cATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgccgcc                                                                                      <  1:615877/151‑1 (MQ=255)

                                                             aTTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGccgccg                                                                                     <  4:43358/151‑1 (MQ=255)

                                                               tGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGccgccg                                                                                     >  1:601241/1‑149 (MQ=255)

                                                               tGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCggg                                                                                   >  1:1361703/1‑151 (MQ=255)

                                                                ggTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCggg                                                                                   <  1:184216/150‑1 (MQ=255)

                                                                ggTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGt                                                                                  >  4:287161/1‑151 (MQ=255)

                                                                  tCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTg                                                                                >  2:1366687/1‑151 (MQ=255)

                                                                    aGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgca                                                                              <  2:292307/151‑1 (MQ=255)

                                                                    aGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTg                                                                                <  4:56002/149‑1 (MQ=255)

                                                                      gAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgcagc                                                                            >  2:622778/1‑151 (MQ=255)

                                                                       aaGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTgcagc                                                                            >  1:283742/1‑150 (MQ=255)

                                                                             cAGCTAATGTCCATCGCCACCTCTTTTTGCCCTTAGTTTTGTAATCCCTCTGTCGCGACCCACTGTAACGGCGGCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGAtgt                                                                      <  1:1271507/150‑1 (MQ=255)

                                                                             cAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGAtgtg                                                                     >  4:108451/1‑151 (MQ=255)

                                                                                cTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTGGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGtt                                                                   <  1:1158041/150‑1 (MQ=255)

                                                                                  aaTGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGtttt                                                                 <  4:104706/150‑1 (MQ=255)

                                                                                  aaTGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGttt                                                                  <  3:277004/149‑1 (MQ=255)

                                                                                   aTGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcg                                                               <  3:289416/151‑1 (MQ=255)

                                                                                     gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCTCCGGGTTGCAGCAGATGTGTTTtcgtc                                                             <  4:331179/151‑1 (MQ=255)

                                                                                     gTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                              <  3:579240/150‑1 (MQ=255)

                                                                                       ccATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTtcgtcg                                                            <  2:1579308/150‑1 (MQ=255)

                                                                                        cATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgcc                                                          <  3:403474/151‑1 (MQ=255)

                                                                                         aTCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccg                                                         >  2:733068/1‑151 (MQ=255)

                                                                                           cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                       >  2:472173/1‑151 (MQ=255)

                                                                                           cGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                       <  2:676605/151‑1 (MQ=255)

                                                                                                   atttttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                               >  1:362201/2‑151 (MQ=255)

                                                                                                     ttttGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                              <  1:557795/150‑1 (MQ=255)

                                                                                                         gCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                          <  2:325561/150‑1 (MQ=255)

                                                                                                         gCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                          <  1:678912/150‑1 (MQ=255)

                                                                                                           ccGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                       <  1:524534/151‑1 (MQ=255)

                                                                                                           ccGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                       >  2:37740/1‑151 (MQ=255)

                                                                                                           ccGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                        <  1:16682/150‑1 (MQ=255)

                                                                                                              tAGTTTTGTAATCCTTCTGTCGCGCCCCACTTTATCGGCGCCCAGCCTTTTGGCGCATCCCATTGTTGCCCACTTTTCCCCGACGTGGTTTTCAGGCCGCGGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCAATTTTGTTGGCGCGa                                     <  1:15020/150‑1 (MQ=255)

                                                                                                                gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                    <  4:496480/149‑1 (MQ=255)

                                                                                                                gTTTTGTAATCCTTCGGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCTCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                    <  1:1145360/149‑1 (MQ=255)

                                                                                                                 ttttGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCt                                  >  2:991419/1‑150 (MQ=255)

                                                                                                                  tttGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt                                >  1:1373957/1‑151 (MQ=255)

                                                                                                                    tGTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTc                               <  2:1044835/150‑1 (MQ=255)

                                                                                                                     gTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                              <  2:427951/150‑1 (MQ=255)

                                                                                                                     gTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                              >  2:1055268/1‑150 (MQ=255)

                                                                                                                     gTAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                             >  1:499130/1‑151 (MQ=255)

                                                                                                                      tAATCCTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCt                            >  4:105597/1‑151 (MQ=255)

                                                                                                                          ccTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGcc                         <  2:252490/150‑1 (MQ=255)

                                                                                                                          ccTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                        >  2:200901/1‑151 (MQ=255)

                                                                                                                          ccTTCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCg                        >  1:1293078/1‑151 (MQ=255)

                                                                                                                             tCTGTCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                     >  4:212594/1‑151 (MQ=255)

                                                                                                                                 tCGCGACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGAc                 >  2:467022/1‑151 (MQ=255)

                                                                                                                                  cgcgACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGACGCCATTTTGTTGGCGCGATCTTTCACTGCCGCATTGACg                >  2:1233741/1‑151 (MQ=255)

                                                                                                                                   gcgACCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt               >  2:901301/1‑151 (MQ=255)

                                                                                                                                      aCCCACTGTAACGGCTCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc             >  1:1259179/1‑150 (MQ=255)

                                                                                                                                      aCCCACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa            <  2:220786/151‑1 (MQ=255)

                                                                                                                                       cccACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc             >  4:276063/1‑149 (MQ=255)

                                                                                                                                        ccACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGc          >  1:520839/1‑151 (MQ=255)

                                                                                                                                        ccACTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCACATTGACGTACAGc          >  1:287453/1‑151 (MQ=255)

                                                                                                                                          aCTGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgg        <  4:326809/151‑1 (MQ=255)

                                                                                                                                            tGTAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCggg       >  3:525530/1‑150 (MQ=255)

                                                                                                                                              tAACGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc    >  2:1723680/1‑151 (MQ=255)

                                                                                                                                               aaCGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc    >  3:171080/1‑150 (MQ=255)

                                                                                                                                               aaCGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACa   <  1:1538615/151‑1 (MQ=255)

                                                                                                                                                aCGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAAc    <  1:170216/149‑1 (MQ=255)

                                                                                                                                                 cGGCGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAg  <  1:335537/150‑1 (MQ=255)

CGGTGGTGCTGACATCATATTTTTCCACCAGCTTTTTCTCCCGGTCATAGGTGTGCTGAACATTGGTCAGGAAGTGCCAGCTAATGTCCATCGCCACCTCTTTTTGCCCGTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAG  >  NC_000913/1245867‑1246161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`