BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,391	T→C	T419A (ACA→GCA)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,391	0	T	C	99.2%	464.3 / NA	126	T419A (ACA→GCA)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (70/55); total (71/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

CGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTC  >  NC_000913/1307243‑1307537

cGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTgcgc                                                                                                                                                  >  3:159376/1‑151 (MQ=255)

  ttttgcccATAAACGGGCATCGGGAAGCCGTAAACGGGAAATAAAACCGCCCTG‑ACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCa                                                                                                                                                <  3:259557/144‑1 (MQ=255)

   tttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCa                                                                                                                                                >  3:155166/1‑150 (MQ=255)

   tttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCa                                                                                                                                                <  2:407492/150‑1 (MQ=255)

    ttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAAc                                                                                                                                              >  2:966980/1‑151 (MQ=255)

    ttAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAAc                                                                                                                                              >  1:386379/1‑151 (MQ=255)

       gCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAg                                                                                                                                           <  2:996327/151‑1 (MQ=255)

         gataagggggaagcGAGCAGGGGGGAAAGGGAAAGATAATAGTGTTAAACGGCGGGGGGGTCAGCACAAAAAAAAAAATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTATGCATATCAAGGTTAACTGTGCCAAACAGAc                                                                                                                                         >  1:58191/14‑151 (MQ=255)

          aTAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACt                                                                                                                                        <  1:1037957/151‑1 (MQ=255)

            aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACttg                                                                                                                                      <  2:846616/151‑2 (MQ=255)

            aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACtt                                                                                                                                       <  1:371773/150‑1 (MQ=255)

                 ggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCg                                                                                                                                 >  2:298387/1‑151 (MQ=255)

                 ggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCg                                                                                                                                 <  4:459463/151‑1 (MQ=255)

                  gCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGc                                                                                                                                <  2:607962/151‑1 (MQ=255)

                       gAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCg                                                                                                                           >  1:1638260/1‑151 (MQ=255)

                       gAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCg                                                                                                                           <  1:1426548/151‑1 (MQ=255)

                        aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGa                                                                                                                          <  2:415414/151‑1 (MQ=255)

                         gCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGAc                                                                                                                         <  2:1497995/151‑1 (MQ=255)

                          cAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                                                        >  2:1267026/1‑151 (MQ=255)

                           aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATGGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                                                        >  2:107172/1‑150 (MQ=255)

                            gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                                                        >  1:1706840/1‑149 (MQ=255)

                            gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGCAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                                                        >  2:99289/1‑149 (MQ=255)

                             aCGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCa                                                                                                                       >  1:1356649/1‑149 (MQ=255)

                              cGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCa                                                                                                                    <  4:388554/151‑1 (MQ=255)

                                 gAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCCTCGACCAGCACGc                                                                                                                 >  1:1118627/1‑151 (MQ=255)

                                 gAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGCCCAGCACg                                                                                                                  <  2:1229530/150‑1 (MQ=255)

                                 gAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc                                                                                                                 >  1:871860/1‑151 (MQ=255)

                                     gcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCGCCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCtt                                                                                                             <  1:520258/151‑1 (MQ=255)

                                      cgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTg                                                                                                            <  1:179345/151‑1 (MQ=255)

                                      cgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTg                                                                                                            <  2:834503/151‑1 (MQ=255)

                                        aaaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                                                          >  1:576835/1‑151 (MQ=255)

                                        aaaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGGAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                                                          >  1:943499/1‑151 (MQ=255)

                                          atatAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTAt                                                                                                        >  2:600779/1‑151 (MQ=255)

                                               aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGt                                                                                                   <  2:468719/151‑1 (MQ=255)

                                               aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGt                                                                                                   >  3:385775/1‑151 (MQ=255)

                                               aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGt                                                                                                   <  2:97923/151‑1 (MQ=255)

                                                  gTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAc                                                                                                <  2:1316439/151‑1 (MQ=255)

                                                  gTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAc                                                                                                >  2:302890/1‑151 (MQ=255)

                                                     cTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAcc                                                                                               <  2:1317571/149‑1 (MQ=255)

                                                      tGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGc                                                                                            >  1:8511/1‑151 (MQ=255)

                                                        aaCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGGAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCCGTAACCCGcc                                                                                           >  2:99490/1‑150 (MQ=255)

                                                         aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCtt                                                                                         >  2:804184/1‑151 (MQ=255)

                                                            gcggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaa                                                                                      <  2:900435/151‑1 (MQ=255)

                                                              ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                                                                    <  2:888306/151‑1 (MQ=255)

                                                              ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                                                                    >  2:172703/1‑151 (MQ=255)

                                                                    gTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                                                                              <  2:446001/151‑1 (MQ=255)

                                                                     tCAGCATCAAAACCTATATCGACGATTGCCAGGGTAATCCCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTCCGCCATCGACCATCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                                                                              <  1:1036133/150‑1 (MQ=255)

                                                                      cAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAt                                                                            >  2:612578/1‑151 (MQ=255)

                                                                      cAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAt                                                                            >  4:111542/1‑151 (MQ=255)

                                                                      cAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAt                                                                            <  1:830040/151‑1 (MQ=255)

                                                                       aGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtt                                                                           <  3:86431/151‑1 (MQ=255)

                                                                       aGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtt                                                                           >  3:11768/1‑151 (MQ=255)

                                                                        gCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAttt                                                                          >  1:967631/1‑151 (MQ=255)

                                                                          aCCAAATCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                                                                        <  1:1730167/151‑1 (MQ=255)

                                                                                 ccTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAcccc                                                                   >  3:353747/1‑149 (MQ=255)

                                                                                  cTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagc                                                                >  3:557373/1‑151 (MQ=255)

                                                                                    ttATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagc                                                                <  2:1020378/149‑1 (MQ=255)

                                                                                       tcgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                                                            >  2:308185/1‑150 (MQ=255)

                                                                                         gtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc                                                         >  1:63908/1‑151 (MQ=255)

                                                                                          tcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc                                                         >  4:426061/1‑150 (MQ=255)

                                                                                             aTTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt                                                     <  3:415837/151‑1 (MQ=255)

                                                                                             aTTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt                                                     <  1:93767/151‑1 (MQ=255)

                                                                                             aTTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt                                                     >  1:53077/1‑151 (MQ=255)

                                                                                              ttGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTc                                                    >  1:1145236/1‑151 (MQ=255)

                                                                                                gCCAGGGTAATCTCGAAATTTAGCCACAGTCTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTcc                                                   <  2:1014417/150‑1 (MQ=255)

                                                                                                gCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTcc                                                   <  2:1679085/150‑1 (MQ=255)

                                                                                                 ccAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt                                                 >  1:549581/1‑151 (MQ=255)

                                                                                                     ggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa                                               >  3:556971/1‑149 (MQ=255)

                                                                                                        aaTCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAAt                                          >  2:836248/1‑151 (MQ=255)

                                                                                                         aTCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATg                                         >  2:1441119/1‑151 (MQ=255)

                                                                                                           ctcGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATgcg                                       >  1:1571026/1‑151 (MQ=255)

                                                                                                             cGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATgcg                                       <  2:1601229/149‑1 (MQ=255)

                                                                                                             cGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATg                                         <  1:910048/147‑1 (MQ=255)

                                                                                                              gAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGcgcg                                     >  1:824146/1‑150 (MQ=255)

                                                                                                                aaTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACt                                  >  2:145727/1‑151 (MQ=255)

                                                                                                                aaTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACt                                  >  2:1611045/1‑151 (MQ=255)

                                                                                                                aaTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACt                                  >  2:410279/1‑151 (MQ=255)

                                                                                                                aaTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACt                                  <  4:150449/151‑1 (MQ=255)

                                                                                                                 aTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTg                                 <  4:304174/151‑1 (MQ=255)

                                                                                                                   ttAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcag                                                              <  4:74012/120‑1 (MQ=255)

                                                                                                                   ttAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTgg                                <  2:1240725/150‑1 (MQ=255)

                                                                                                                   ttAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGc                               >  4:280595/1‑151 (MQ=255)

                                                                                                                      gCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCa                            <  2:541133/151‑1 (MQ=255)

                                                                                                                       ccACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGccc                             >  1:1466685/1‑149 (MQ=255)

                                                                                                                       ccACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGccc                             >  3:547299/1‑149 (MQ=255)

                                                                                                                        cacaGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGc                          >  2:330008/1‑151 (MQ=255)

                                                                                                                          caGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCg                        >  4:118528/1‑151 (MQ=255)

                                                                                                                             aCTACGCATATCAAGGTTAACTGCGCCACCCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGAc                      <  1:512640/150‑1 (MQ=255)

                                                                                                                                 cGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCa                 <  2:1253519/151‑1 (MQ=255)

                                                                                                                                  gCAGATCAAGGTTAACTGCACCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGTTATGCAGTCACCCGCCTTCAAACTGATAAACTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCCCGACTGGCCCAGCCGACCAGCAt                <  1:311591/151‑1 (MQ=255)

                                                                                                                                   cATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATc               <  1:561324/151‑1 (MQ=255)

                                                                                                                                    atatCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCg              >  3:442787/1‑151 (MQ=255)

                                                                                                                                       tCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGt           >  2:1276496/1‑151 (MQ=255)

                                                                                                                                         aaGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTc          >  2:864498/1‑150 (MQ=255)

                                                                                                                                          aGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAt        <  4:115992/151‑1 (MQ=255)

                                                                                                                                           ggTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtt       >  2:705674/1‑151 (MQ=255)

                                                                                                                                             ttAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtttt     >  2:606325/1‑151 (MQ=255)

                                                                                                                                               aaCTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtttttt   <  2:461953/151‑1 (MQ=255)

                                                                                                                                               aaCTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtttttt   >  2:1390167/1‑151 (MQ=255)

                                                                                                                                                aCTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTc  <  2:871971/151‑1 (MQ=255)

CGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTC  >  NC_000913/1307243‑1307537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`