Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,336,738 T→C I303T (ATT→ACT)  acnA → aconitate hydratase 1; aconitase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,336,7380TC99.2% 467.3 / ‑4.1 125I303T (ATT→ACT) acnAaconitate hydratase 1; aconitase A
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (66/58);  total (67/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 126 total aligned reads displayed.

CCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTA  >  NC_000913/1336594‑1336884
                                                                                                                                                |                                                                                                                                                  
ccGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCa                                                                                                                                               <  2:293992/150‑1 (MQ=255)
  ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAat                                                                                                                                             >  2:1473941/1‑150 (MQ=255)
  ggAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAACTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAat                                                                                                                                             >  1:862064/1‑150 (MQ=255)
    aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAatat                                                                                                                                           <  2:782396/150‑1 (MQ=255)
    aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAatat                                                                                                                                           >  1:1446851/1‑150 (MQ=255)
      aaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTc                                                                                                                                        >  4:473979/1‑151 (MQ=255)
         tACGTGAAGGTATTGCCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGc                                                                                                                                      <  2:298370/150‑1 (MQ=255)
         tACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGcc                                                                                                                                     >  2:1195710/1‑151 (MQ=255)
         tACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGcc                                                                                                                                     >  1:872641/1‑151 (MQ=255)
          aCGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCa                                                                                                                                    <  2:935500/151‑1 (MQ=255)
           cGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGcc                                                                                                                                     >  2:153072/1‑149 (MQ=255)
           cGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCa                                                                                                                                    <  2:1313003/150‑1 (MQ=255)
            gTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAg                                                                                                                                   <  4:250373/150‑1 (MQ=255)
                aGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAatat                                                                                                                              <  3:478964/151‑1 (MQ=255)
                 ggTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATg                                                                                                                             >  2:47493/1‑151 (MQ=255)
                  gTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATgg                                                                                                                            <  2:390715/151‑1 (MQ=255)
                      tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGcc                                                                                                                        >  2:884183/1‑151 (MQ=255)
                           ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                                                                   <  4:53994/151‑1 (MQ=255)
                            cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCtgt                                                                                                                  <  1:1056366/151‑1 (MQ=255)
                            cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCtgt                                                                                                                  >  1:1551723/1‑151 (MQ=255)
                            cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                                                                   >  1:1165252/1‑150 (MQ=255)
                              caGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                                                                >  2:840979/1‑151 (MQ=255)
                              caGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                                                                <  4:30701/151‑1 (MQ=255)
                              caGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTgg                                                                                                                <  1:715941/151‑1 (MQ=255)
                               aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGc                                                                                                               >  3:218127/1‑151 (MQ=255)
                                 aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGctt                                                                                                             >  2:1608482/1‑151 (MQ=255)
                                 aCCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGctt                                                                                                             <  1:1218629/151‑1 (MQ=255)
                                  ccTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                                                            >  2:1728679/1‑151 (MQ=255)
                                  ccTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                                                            >  2:1406641/1‑151 (MQ=255)
                                     ggTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCttcttc                                                                                                         >  3:549561/1‑151 (MQ=255)
                                      gTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTcc                                                                                                        <  1:1657096/151‑1 (MQ=255)
                                       ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTccc                                                                                                       >  2:1556316/1‑151 (MQ=255)
                                           cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATc                                                                                                   >  1:618207/1‑151 (MQ=255)
                                            aCTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCg                                                                                                  >  1:700861/1‑151 (MQ=255)
                                              tGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                                                                >  2:680413/1‑151 (MQ=255)
                                                 tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCt                                                                                             >  2:1009140/1‑151 (MQ=255)
                                                 tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCt                                                                                             >  1:1372899/1‑151 (MQ=255)
                                                   cccAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                                                           <  4:65365/151‑1 (MQ=255)
                                                   cccAAATGCTGCGCAAACAGGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                                                           <  1:1329887/151‑1 (MQ=255)
                                                     cAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                                                          >  1:1570798/1‑150 (MQ=255)
                                                            tgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCg                                                                                   >  2:1384951/1‑150 (MQ=255)
                                                            tgcGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCg                                                                                   >  2:1501986/1‑150 (MQ=255)
                                                             gcgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGa                                                                                  <  1:29520/150‑1 (MQ=255)
                                                              cgcAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAtt                                                                                >  1:695649/1‑151 (MQ=255)
                                                                 aaaCATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTa                                                                               >  1:1735260/1‑149 (MQ=255)
                                                                    cATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGc                                                                          >  2:1013127/1‑151 (MQ=255)
                                                                       ggCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGtt                                                                       <  4:330783/151‑1 (MQ=255)
                                                                        gCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTATCCCTCGATTACATGCGttt                                                                      <  1:225985/151‑1 (MQ=255)
                                                                        gCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGttt                                                                      <  2:1507463/151‑1 (MQ=255)
                                                                          gtggtgGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTaa                                                                    <  2:588066/151‑1 (MQ=255)
                                                                             gtggGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCg                                                                 >  2:654959/1‑151 (MQ=255)
                                                                              tggGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCgg                                                                >  1:1672457/1‑151 (MQ=255)
                                                                                 ggAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcg                                                             <  4:540965/151‑1 (MQ=255)
                                                                                   aaaTTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                                                            >  1:1112586/1‑150 (MQ=255)
                                                                                        cgtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGaa                                                      <  4:133817/151‑1 (MQ=255)
                                                                                        cgtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                                                       >  2:941393/1‑150 (MQ=255)
                                                                                        cgtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                                                       <  2:774446/150‑1 (MQ=255)
                                                                                         gtcgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                                                       >  2:1156485/1‑149 (MQ=255)
                                                                                           cgAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa                                                       >  2:100950/1‑147 (MQ=255)
                                                                                            gAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgc                                                              >  2:1117369/1‑139 (MQ=255)
                                                                                            gAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgc                                                              <  1:1117235/139‑1 (MQ=255)
                                                                                             aaTTTTATGGTGATGGTCTGGTTTCACTACGGTGGGCGGACCGCGCCACCACTGCCAATATGTTGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc                                                  <  1:1256647/150‑1 (MQ=255)
                                                                                             aaTTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCa                                                 <  4:358360/151‑1 (MQ=255)
                                                                                                 ttATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTc                                             >  3:275496/1‑151 (MQ=255)
                                                                                                  tATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTc                                             <  2:1570959/150‑1 (MQ=255)
                                                                                                  tATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg                                            >  3:436312/1‑151 (MQ=255)
                                                                                                     ggTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAg                                          <  1:47484/150‑1 (MQ=255)
                                                                                                      gTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGtt                                        <  2:863485/151‑1 (MQ=255)
                                                                                                        gATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTg                                       <  1:1328785/150‑1 (MQ=255)
                                                                                                           ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                   <  4:437156/151‑1 (MQ=255)
                                                                                                           ggTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                   <  1:1188113/151‑1 (MQ=255)
                                                                                                            gTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                   >  3:77447/1‑150 (MQ=255)
                                                                                                            gTCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGa                                  <  2:1443209/151‑1 (MQ=255)
                                                                                                             tCTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGa                                  >  1:243907/1‑150 (MQ=255)
                                                                                                              cTGGATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATCTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGTGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaa                                 >  1:216693/1‑150 (MQ=255)
                                                                                                                ggATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaaaaa                              <  3:48121/151‑1 (MQ=255)
                                                                                                                ggATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaaaaa                              <  1:1219033/151‑1 (MQ=255)
                                                                                                                ggATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaaaa                               >  4:104214/1‑150 (MQ=255)
                                                                                                                ggATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaaaa                               >  4:553944/1‑150 (MQ=255)
                                                                                                                ggATTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGaa                                 <  3:192396/148‑1 (MQ=255)
                                                                                                                  aTTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAat                             >  2:1058890/1‑150 (MQ=255)
                                                                                                                  aTTCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAat                             >  4:466813/1‑150 (MQ=255)
                                                                                                                    tCACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAatat                           <  2:1232999/150‑1 (MQ=255)
                                                                                                                     cACTACCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGc                         >  2:453254/1‑151 (MQ=255)
                                                                                                                         aCCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCaaa                     <  2:1276471/151‑1 (MQ=255)
                                                                                                                         aCCGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCaaa                     <  2:1149559/151‑1 (MQ=255)
                                                                                                                          ccGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAg                    <  2:630184/151‑1 (MQ=255)
                                                                                                                           cGTTGGCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAg                    >  1:169186/1‑150 (MQ=255)
                                                                                                                               ggCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAg               <  1:153045/151‑1 (MQ=255)
                                                                                                                                gCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAgg              <  3:466830/151‑1 (MQ=255)
                                                                                                                                gCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAgg              <  2:290968/151‑1 (MQ=255)
                                                                                                                                gCGGATCGCGCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAgg              >  1:499162/1‑151 (MQ=255)
                                                                                                                                  ggATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGc            >  2:741824/1‑151 (MQ=255)
                                                                                                                                      cgcgCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAtgt        >  2:1065409/1‑151 (MQ=255)
                                                                                                                                      cgcgCCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAtg         >  1:1093976/1‑150 (MQ=255)
                                                                                                                                       gcgcCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCAtgtg       <  2:548576/151‑1 (MQ=255)
                                                                                                                                         gcCACCACTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGc     <  1:221368/151‑1 (MQ=255)
                                                                                                                                            accaCTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTa  <  1:384293/151‑1 (MQ=255)
                                                                                                                                             ccaCTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTa  >  3:195160/1‑150 (MQ=255)
                                                                                                                                             ccaCTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTa  >  1:769158/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTA  >  NC_000913/1336594‑1336884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: