Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 240,865 G→A A813A (GCC→GCT)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913240,8650GA100.0% 587.5 / NA 147A813A (GCC→GCT) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (81/66);  total (81/66)

Only 100 of 148 total aligned reads displayed.

AATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTC  >  NC_000913/240718‑241012
                                                                                                                                                   |                                                                                                                                                   
aaTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCt                                                                                                                                                  >  4:22193/1‑151 (MQ=255)
aaTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCt                                                                                                                                                  >  3:303122/1‑151 (MQ=255)
aaTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCt                                                                                                                                                  >  3:11941/1‑151 (MQ=255)
  tgtgAACGATGCGCTTTCGATTGATGGTAAAACGGGGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTc                                                                                                                                                >  3:221513/1‑151 (MQ=255)
   gtgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTc                                                                                                                                                <  1:600467/150‑1 (MQ=255)
   gtgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTc                                                                                                                                                >  2:1582211/1‑150 (MQ=255)
   gtgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTc                                                                                                                                                >  1:312196/1‑150 (MQ=255)
      aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACt                                                                                                                                            >  2:1383499/1‑151 (MQ=255)
       aCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACtt                                                                                                                                           <  2:1635959/151‑1 (MQ=255)
       aCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACtt                                                                                                                                           >  2:914825/1‑151 (MQ=255)
        cGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACttt                                                                                                                                          <  2:925466/151‑1 (MQ=255)
         gATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGATCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTc                                                                                                                                         <  2:190239/151‑1 (MQ=255)
         gATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTc                                                                                                                                         >  2:314504/1‑151 (MQ=255)
          aTGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTcc                                                                                                                                        <  4:113282/151‑1 (MQ=255)
          aTGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTcc                                                                                                                                        >  2:1329575/1‑151 (MQ=255)
           tGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTcc                                                                                                                                        <  1:823700/150‑1 (MQ=255)
            gcgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCg                                                                                                                                       <  3:531722/150‑1 (MQ=255)
            gcgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGc                                                                                                                                      >  1:1216523/1‑151 (MQ=255)
            gcgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGc                                                                                                                                      >  2:1337369/1‑151 (MQ=255)
             cgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGc                                                                                                                                      >  2:476645/1‑150 (MQ=255)
             cgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCa                                                                                                                                     <  3:549151/151‑1 (MQ=255)
              gcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCAc                                                                                                                                    >  2:442115/1‑151 (MQ=255)
              gcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCAc                                                                                                                                    >  2:800802/1‑151 (MQ=255)
                 ttCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACttt                                                                                                                                 >  2:383599/1‑151 (MQ=255)
                 ttCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACtt                                                                                                                                  <  2:1139294/150‑1 (MQ=255)
                   cGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTtc                                                                                                                                <  2:262802/150‑1 (MQ=255)
                             tAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACtt                                                                                                                     >  1:491110/1‑151 (MQ=255)
                              aaaaCGGTGTTGTTCGCGGCGCTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACttt                                                                                                                    <  1:1467147/151‑1 (MQ=255)
                               aaaCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTa                                                                                                                   <  1:1585062/151‑1 (MQ=255)
                                   ggTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCgg                                                                                                               <  4:593829/151‑1 (MQ=255)
                                    gtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGc                                                                                                              <  4:174478/151‑1 (MQ=255)
                                    gtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGc                                                                                                              >  2:1304600/1‑151 (MQ=255)
                                          ttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                                        >  2:210063/1‑151 (MQ=255)
                                            cgcgGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGc                                                                                                      >  1:614014/1‑151 (MQ=255)
                                              cggcgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGcc                                                                                                     >  1:910922/1‑150 (MQ=255)
                                                 cgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                                                  >  1:649780/1‑150 (MQ=255)
                                                  gTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctc                                                                                                >  2:1291304/1‑151 (MQ=255)
                                                    tGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctct                                                                                               <  1:1420638/150‑1 (MQ=255)
                                                     gACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTc                                                                                             <  4:441468/151‑1 (MQ=255)
                                                       ccGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCg                                                                                           <  4:587547/151‑1 (MQ=255)
                                                       ccGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCg                                                                                           >  2:1319933/1‑151 (MQ=255)
                                                        cGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                                          >  1:468828/1‑151 (MQ=255)
                                                           cAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATc                                                                                       >  1:240355/1‑151 (MQ=255)
                                                               cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaa                                                                                     >  2:438473/1‑149 (MQ=255)
                                                                 ggAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtc                                                                                 >  2:1145604/1‑151 (MQ=255)
                                                                   aaaaCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtcat                                                                               <  1:631952/151‑1 (MQ=255)
                                                                       ccATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAAc                                                                           <  1:554893/151‑1 (MQ=255)
                                                                        cATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                                          >  2:1568851/1‑151 (MQ=255)
                                                                            cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                                                       >  1:176844/1‑150 (MQ=255)
                                                                             ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                                                       >  2:1631156/1‑149 (MQ=255)
                                                                             ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAAt                                                                     >  2:632818/1‑151 (MQ=255)
                                                                             ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAAt                                                                     <  3:506553/151‑1 (MQ=255)
                                                                              gATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATa                                                                    <  1:1402022/151‑1 (MQ=255)
                                                                                   cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                                               >  1:1013763/1‑151 (MQ=255)
                                                                                    tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                                               >  1:1073811/1‑150 (MQ=255)
                                                                                     ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                              >  1:1484854/1‑150 (MQ=255)
                                                                                      tAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                              >  1:911695/1‑149 (MQ=255)
                                                                                       aaTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                              >  2:73993/1‑148 (MQ=255)
                                                                                       aaTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGa                                                           <  1:118879/151‑1 (MQ=255)
                                                                                          tttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACg                                                         >  4:44230/1‑150 (MQ=255)
                                                                                           ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                                                       >  2:489397/1‑151 (MQ=255)
                                                                                             aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCt                                                      <  1:699852/150‑1 (MQ=255)
                                                                                             aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCt                                                      <  3:5042/150‑1 (MQ=255)
                                                                                              aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCttt                                                    >  3:380319/1‑151 (MQ=255)
                                                                                              aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCttt                                                    <  1:606348/151‑1 (MQ=255)
                                                                                                taatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                                                  >  4:487268/1‑151 (MQ=255)
                                                                                                 aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttctt                                                 >  2:729156/1‑151 (MQ=255)
                                                                                                  atTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttcttc                                                <  2:1043392/151‑1 (MQ=255)
                                                                                                    tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttcttc                                                >  1:1733460/1‑149 (MQ=255)
                                                                                                       cGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCtt                                           <  1:1183841/151‑1 (MQ=255)
                                                                                                       cGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCtt                                           <  1:1319789/151‑1 (MQ=255)
                                                                                                        ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                          <  1:397826/151‑1 (MQ=255)
                                                                                                         gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                                         <  1:948023/151‑1 (MQ=255)
                                                                                                         gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                                         >  2:869024/1‑151 (MQ=255)
                                                                                                          aTAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                                         >  1:990370/1‑150 (MQ=255)
                                                                                                          aTAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGCCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                                         >  1:1065404/1‑150 (MQ=255)
                                                                                                            aaaGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACt                                                                  >  2:563840/1‑123 (MQ=255)
                                                                                                              aGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAcc                                      >  1:1631866/1‑149 (MQ=255)
                                                                                                               gAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAcc                                      >  2:61922/1‑148 (MQ=255)
                                                                                                                 aaCGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAAt                                 <  1:400492/151‑1 (MQ=255)
                                                                                                                  aCGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATa                                <  2:1522665/151‑1 (MQ=255)
                                                                                                                     gAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCg                             >  1:446042/1‑151 (MQ=255)
                                                                                                                       gCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGc                           >  1:67325/1‑151 (MQ=255)
                                                                                                                          tttCGGCTCCGTTATTCATTTACGCAGCTTCGACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTc                        >  2:264108/1‑151 (MQ=255)
                                                                                                                           ttCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTc                        <  1:259587/150‑1 (MQ=255)
                                                                                                                           ttCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTc                        <  1:782478/150‑1 (MQ=255)
                                                                                                                            tCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCAt                      >  4:326437/1‑151 (MQ=255)
                                                                                                                            tCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCGTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCa                       <  1:783662/150‑1 (MQ=255)
                                                                                                                             cGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                     <  2:102973/151‑1 (MQ=255)
                                                                                                                              ggCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCt                    <  2:1424194/151‑1 (MQ=255)
                                                                                                                                 tCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                  >  3:152961/1‑150 (MQ=255)
                                                                                                                                  ccGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTat                <  2:1307019/151‑1 (MQ=255)
                                                                                                                                  ccGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTa                 <  4:547935/150‑1 (MQ=255)
                                                                                                                                   cGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCCGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatc               <  3:478337/151‑1 (MQ=255)
                                                                                                                                         tCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatcaatca          <  1:800698/150‑1 (MQ=255)
                                                                                                                                          cATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGc        <  2:1550158/151‑1 (MQ=255)
                                                                                                                                           aTTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcc       >  2:1448594/1‑151 (MQ=255)
                                                                                                                                              tACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCt    >  1:303710/1‑151 (MQ=255)
                                                                                                                                               aCGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCt    <  2:765741/150‑1 (MQ=255)
                                                                                                                                                cgcaGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTc  <  2:471076/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTC  >  NC_000913/240718‑241012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑