Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,630,461 A→G N151N (AAT→AAC ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,630,4610AG100.0% 412.1 / NA 110N151N (AAT→AACydfIputative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/57);  total (53/57)

Only 100 of 110 total aligned reads displayed.

CGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATC  >  NC_000913/1630316‑1630607
                                                                                                                                                 |                                                                                                                                                  
cGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGtttt                                                                                                                                                >  2:236945/1‑150 (MQ=255)
        gTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCgg                                                                                                                                       <  3:365643/151‑1 (MQ=255)
         tGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGc                                                                                                                                      >  4:146927/1‑151 (MQ=255)
          gACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGc                                                                                                                                      >  2:1300392/1‑150 (MQ=255)
            cAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGTGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGc                                                                                                                                   <  2:1485646/151‑1 (MQ=255)
               cGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTAc                                                                                                                                >  2:1371455/1‑151 (MQ=255)
                   aCGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATc                                                                                                                            <  2:1226943/151‑1 (MQ=255)
                      cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                         >  1:1616564/1‑151 (MQ=255)
                      cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGGTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                         >  1:955499/1‑151 (MQ=255)
                       aTAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGt                                                                                                                        <  1:1283468/151‑1 (MQ=255)
                       aTAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGt                                                                                                                        >  1:238486/1‑151 (MQ=255)
                         aaCATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGa                                                                                                                      <  2:1736215/151‑1 (MQ=255)
                              gACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGa                                                                                                                  <  2:1314475/150‑1 (MQ=255)
                              gACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCGTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGa                                                                                                                  <  1:465357/150‑1 (MQ=255)
                                 cGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAt                                                                                                              >  2:769448/1‑151 (MQ=255)
                                  gTTTTCTGGCATGTTGTCACATGACATGACGGTTAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAtt                                                                                                             >  2:41131/1‑151 (MQ=255)
                                  gTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAtt                                                                                                             >  1:382471/1‑151 (MQ=255)
                                   ttttCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAtt                                                                                                             >  1:863298/1‑150 (MQ=255)
                                    tttCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTa                                                                                                            >  1:880879/1‑150 (MQ=255)
                                      tCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAAc                                                                                                          <  3:525170/150‑1 (MQ=255)
                                      tCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACt                                                                                                         >  1:226800/1‑151 (MQ=255)
                                       cTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACt                                                                                                         >  1:1461342/1‑150 (MQ=255)
                                         ggCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGt                                                                                                       <  1:613782/150‑1 (MQ=255)
                                         ggCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTc                                                                                                      >  3:424805/1‑151 (MQ=255)
                                            aTGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCgg                                                                                                   <  1:1441239/151‑1 (MQ=255)
                                               ttgtCACATGACCTGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCg                                                                                                >  3:218608/1‑151 (MQ=255)
                                                tgtCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGc                                                                                               >  1:677255/1‑151 (MQ=255)
                                                   cacaTGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                            >  2:52335/1‑151 (MQ=255)
                                                     catgacatgaCGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGga                                                                                           <  1:37188/150‑1 (MQ=255)
                                                      atgacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                            >  1:440773/1‑148 (MQ=255)
                                                       tgacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGgag                                                                                          >  2:60917/1‑149 (MQ=255)
                                                        gacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGagag                                                                                        <  1:1732700/150‑1 (MQ=255)
                                                        gacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGagag                                                                                        <  2:1657680/150‑1 (MQ=255)
                                                        gacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGt                                                                                       <  2:1097375/151‑1 (MQ=255)
                                                        gacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGt                                                                                       <  4:13380/151‑1 (MQ=255)
                                                         acatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTg                                                                                      >  1:1599300/1‑151 (MQ=255)
                                                          catgacGGTAAACGCGCGAAGCCCTGCCGCTTTACCGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGGCGGCAGCTACCATCGGGTGATCGGGCATTAACTGTCCGGTCGCCGGAGAGTgg                                                                                     <  1:1589998/151‑1 (MQ=255)
                                                             gacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGaa                                                                                   <  3:368727/150‑1 (MQ=255)
                                                              acGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAt                                                                                 <  3:338095/151‑1 (MQ=255)
                                                              acGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAt                                                                                 <  1:1253646/151‑1 (MQ=255)
                                                               cGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATa                                                                                <  2:1456136/151‑1 (MQ=255)
                                                                ggTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAc                                                                               >  2:1240472/1‑151 (MQ=255)
                                                                 gTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAcc                                                                              <  2:819679/151‑1 (MQ=255)
                                                                  tAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAcc                                                                              <  2:238485/150‑1 (MQ=255)
                                                                   aaaTGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCt                                                                            >  4:346465/1‑151 (MQ=255)
                                                                   aaaTGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCt                                                                            >  1:1433381/1‑151 (MQ=255)
                                                                       gCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCttttt                                                                        <  1:1446335/151‑1 (MQ=255)
                                                                       gCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCttttt                                                                        >  1:830198/1‑151 (MQ=255)
                                                                       gCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCtttt                                                                         <  1:715235/150‑1 (MQ=255)
                                                                         gggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                                      <  4:471011/151‑1 (MQ=255)
                                                                          ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCgg                                                                     <  3:191774/151‑1 (MQ=255)
                                                                          ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCgg                                                                     >  1:158612/1‑151 (MQ=255)
                                                                          ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCgg                                                                     <  2:1670324/151‑1 (MQ=255)
                                                                          ggAAGTACTGCCGATTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTGCCATCGGGTGATCGAGCATTAACTGTACGGTCGCCGGAGAGTGGAAATACCCTTTTTCgg                                                                     >  1:298037/1‑151 (MQ=255)
                                                                           gAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGt                                                                    <  2:1270034/151‑1 (MQ=255)
                                                                               tCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGAtt                                                                >  1:1499770/1‑151 (MQ=255)
                                                                                 cTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                              >  3:540819/1‑151 (MQ=255)
                                                                                 cTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                              >  2:406681/1‑151 (MQ=255)
                                                                                  tGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTc                                                             >  1:1680296/1‑151 (MQ=255)
                                                                                   gCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATTCCCTTTTTCGGTGACTGTc                                                             <  1:1695778/150‑1 (MQ=255)
                                                                                    ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                           <  4:218590/151‑1 (MQ=255)
                                                                                      gCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGGTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                           >  2:847894/1‑149 (MQ=255)
                                                                                         ttACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagaga                                                        <  2:1307467/149‑1 (MQ=255)
                                                                                          tACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAgagaga                                                      >  1:104942/1‑150 (MQ=255)
                                                                                          tACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGCTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAgagaga                                                      <  1:1275689/150‑1 (MQ=255)
                                                                                                gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgc                                               >  1:250242/1‑151 (MQ=255)
                                                                                                gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgc                                               >  1:1362629/1‑151 (MQ=255)
                                                                                                gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGAGATCGAGCATTAACTGTCCGGTCCCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGTGAGACAATc                                                 >  4:236974/1‑149 (MQ=255)
                                                                                                 aGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGTCAATcgcg                                              <  1:355638/151‑1 (MQ=255)
                                                                                                 aGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgcg                                              <  2:774474/151‑1 (MQ=255)
                                                                                                   ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                            <  1:236905/151‑1 (MQ=255)
                                                                                                   ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                            <  1:1546414/151‑1 (MQ=255)
                                                                                                    cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCttttt                                                                        <  1:1310596/122‑1 (MQ=255)
                                                                                                    cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCttttt                                                                        >  2:1310744/1‑122 (MQ=255)
                                                                                                    cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAtt                                           <  3:344274/151‑1 (MQ=255)
                                                                                                    cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAtt                                           >  3:149381/1‑151 (MQ=255)
                                                                                                       cgcCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGCGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                         >  1:1416987/1‑150 (MQ=255)
                                                                                                            ctacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAATTGTCCGGTCGCCGGAGTGTGGAAATACCCTTTTTCTGTGATTGTCAGAGACACAATCGCGATTTGCGGTTc                                   >  1:892760/3‑151 (MQ=255)
                                                                                                            caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                    >  1:382905/1‑150 (MQ=255)
                                                                                                            caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                    <  4:119938/150‑1 (MQ=255)
                                                                                                              acaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGGGACAATCGCGATTTGCGGTTcac                                 <  2:1090363/151‑1 (MQ=255)
                                                                                                                aaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcacac                               >  3:343550/1‑151 (MQ=255)
                                                                                                                   cACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACAt                             <  3:463272/150‑1 (MQ=255)
                                                                                                                   cACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATc                            >  2:1672321/1‑151 (MQ=255)
                                                                                                                        cTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGc                       >  4:143132/1‑151 (MQ=255)
                                                                                                                         tGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGcc                      >  1:869319/1‑151 (MQ=255)
                                                                                                                              cTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAacac                 >  1:1405622/1‑151 (MQ=255)
                                                                                                                                gTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACgg               >  1:1365115/1‑151 (MQ=255)
                                                                                                                                gTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACgg               <  2:1696886/151‑1 (MQ=255)
                                                                                                                                 ttttCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACgg               <  2:1616727/150‑1 (MQ=255)
                                                                                                                                  tttCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGt              <  2:812154/150‑1 (MQ=255)
                                                                                                                                   ttCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGtt             <  1:1132150/150‑1 (MQ=255)
                                                                                                                                   ttCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGtt             <  2:112569/150‑1 (MQ=255)
                                                                                                                                     cGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTc           <  1:1127351/150‑1 (MQ=255)
                                                                                                                                     cGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCt          <  2:1401870/151‑1 (MQ=255)
                                                                                                                                      ggCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAACCTTTTTCGGTGATTGTCAGAGATACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTc           <  1:110627/149‑1 (MQ=255)
                                                                                                                                        cTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTa         >  2:1559043/1‑149 (MQ=255)
                                                                                                                                          ggtggGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGcc     <  2:440845/151‑1 (MQ=255)
                                                                                                                                          ggtggGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGcc     <  2:1278357/151‑1 (MQ=255)
                                                                                                                                             ggggGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatc  <  1:1645368/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATC  >  NC_000913/1630316‑1630607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: