Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,631,501 C→T pseudogene (785/1284 nt) ydfJ ← pseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,631,5010CT99.2% 507.7 / NA 132pseudogene (785/1284 nt)ydfJpseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (57/74);  minor base G (1/0);  total (58/74)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TAATGGTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATT  >  NC_000913/1631355‑1631648
                                                                                                                                                  |                                                                                                                                                   
tAATGGTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCg                                                                                                                                                   >  1:576398/1‑149 (MQ=255)
  aTGGTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGaa                                                                                                                                               <  1:232070/151‑1 (MQ=255)
  aTGGTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGaa                                                                                                                                               <  1:898103/151‑1 (MQ=255)
     gTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATca                                                                                                                                            <  1:539729/151‑2 (MQ=255)
     gTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCg                                                                                                                                            >  1:638634/1‑151 (MQ=255)
          cGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTg                                                                                                                                       >  1:556269/1‑151 (MQ=255)
           ggggCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTg                                                                                                                                       >  1:257144/1‑150 (MQ=255)
             ggCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGatc                                                                                                                                    <  1:1139424/151‑1 (MQ=255)
              gCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGatca                                                                                                                                   <  1:179821/151‑1 (MQ=255)
               cATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGatcat                                                                                                                                  <  3:15034/151‑1 (MQ=255)
                aTAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGatcatc                                                                                                                                 >  1:1546716/1‑151 (MQ=255)
                  aGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCaa                                                                                                                               <  2:1235380/151‑1 (MQ=255)
                   gCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAAt                                                                                                                              <  2:1001837/151‑1 (MQ=255)
                       ttATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATTCa                                                                                                                           <  1:1442712/150‑1 (MQ=255)
                          tCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTg                                                                                                                       >  3:177997/1‑151 (MQ=255)
                          tCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTg                                                                                                                       >  2:923211/1‑151 (MQ=255)
                             aCAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTg                                                                                                                       <  2:1128548/148‑1 (MQ=255)
                               aaTGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTgg                                                                                                                   >  1:1140892/1‑150 (MQ=255)
                               aaTGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGa                                                                                                                  <  4:453702/151‑1 (MQ=255)
                                 tGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAAt                                                                                                                >  1:1391306/1‑151 (MQ=255)
                                 tGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAAt                                                                                                                >  1:407721/1‑151 (MQ=255)
                                   aTAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTg                                                                                                              <  4:232225/151‑1 (MQ=255)
                                        aaGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTg                                                                                                         <  2:1421622/151‑1 (MQ=255)
                                         aGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGt                                                                                                        >  2:634413/1‑151 (MQ=255)
                                           cATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGtt                                                                                                       >  3:40235/1‑150 (MQ=255)
                                            aTTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTaa                                                                                                     <  4:518068/151‑1 (MQ=255)
                                            aTTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTaa                                                                                                     <  2:1664788/151‑1 (MQ=255)
                                            aTTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTa                                                                                                      >  3:140464/1‑150 (MQ=255)
                                              tGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTaaa                                                                                                    <  4:398904/150‑1 (MQ=255)
                                               ggCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACa                                                                                                  <  2:448368/151‑1 (MQ=255)
                                                 ccATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAAt                                                                                                <  2:1714583/151‑1 (MQ=255)
                                                 ccATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAAt                                                                                                >  1:910948/1‑151 (MQ=255)
                                                  cATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAAt                                                                                                >  1:863554/1‑150 (MQ=255)
                                                   aTGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATaa                                                                                              <  2:466591/151‑1 (MQ=255)
                                                       cAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTc                                                                                          <  2:1023458/151‑1 (MQ=255)
                                                         gCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTg                                                                                        <  2:638724/151‑1 (MQ=255)
                                                          cacaATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGc                                                                                       >  4:187548/1‑151 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    >  2:582759/1‑151 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    <  2:301506/151‑1 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    >  2:1474044/1‑151 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    <  3:326269/151‑1 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    <  1:1269954/151‑1 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    >  1:1280925/1‑151 (MQ=255)
                                                             aaTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    <  1:266860/151‑1 (MQ=255)
                                                              aTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    >  2:413783/1‑150 (MQ=255)
                                                              aTCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACt                                                                                    >  2:1412381/1‑150 (MQ=255)
                                                                 gcgGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAAt                                                                                <  3:334953/151‑1 (MQ=255)
                                                                 gcgGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAAt                                                                                >  2:201947/1‑151 (MQ=255)
                                                                  cgGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAAt                                                                                >  1:530871/1‑150 (MQ=255)
                                                                  cgGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATa                                                                               <  2:1678145/151‑1 (MQ=255)
                                                                   ggAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAg                                                                              <  2:317962/151‑1 (MQ=255)
                                                                    gAGGTATTCATAATAATATACGGCATCCGGCGACCAATGTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACGGATCACCAATGCATCTGTTGGAATTGCTGTGTTAAACAATAAGGGCTGCACTAAATat                                                                              <  1:762175/150‑2 (MQ=255)
                                                                      ggTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCt                                                                           <  3:81146/151‑1 (MQ=255)
                                                                      ggTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCt                                                                           <  4:422285/151‑1 (MQ=255)
                                                                        tATTCATAATAATATACGGGGTGCGGGGACAAATTTTATCGGATAACCAACCAAGGAACGTAATGGACATAAAGTCGAGAATCGAAATGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCAATAAATAGCCtac                                                                         >  1:304492/1‑149 (MQ=255)
                                                                         aTTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGc                                                                         <  3:443253/150‑1 (MQ=255)
                                                                         aTTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGc                                                                         >  2:241431/1‑150 (MQ=255)
                                                                         aTTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCa                                                                        <  2:877352/151‑1 (MQ=255)
                                                                             ataataataTACGGGGTCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGa                                                                    <  4:504155/151‑1 (MQ=255)
                                                                             ataataataTACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGa                                                                    <  3:14072/151‑1 (MQ=255)
                                                                                     ataCGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTg                                                             >  2:562065/1‑150 (MQ=255)
                                                                                     ataCGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTg                                                             <  4:476414/150‑1 (MQ=255)
                                                                                      taCGCGGTCCGGCGACCAATTTTATCGGCTAACCAACCAAGCAACGGAGTGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGATACACAATAACGTGTGCACTAAATATCCTGCAAGGAAAGTCt                                                              >  1:1581825/1‑148 (MQ=255)
                                                                                          ggATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTa                                                        <  1:1005259/150‑1 (MQ=255)
                                                                                             tCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAAccc                                                    >  4:125510/1‑151 (MQ=255)
                                                                                              ccGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCg                                                   <  3:97111/151‑1 (MQ=255)
                                                                                              ccGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCg                                                   >  2:399049/1‑151 (MQ=255)
                                                                                               cGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCgg                                                  <  1:170934/151‑1 (MQ=255)
                                                                                                   gACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGtt                                              >  4:218799/1‑151 (MQ=255)
                                                                                                   gACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGtt                                              <  2:63384/151‑1 (MQ=255)
                                                                                                     ccAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTAc                                            >  4:47935/1‑151 (MQ=255)
                                                                                                     ccAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTAc                                            >  1:1281197/1‑151 (MQ=255)
                                                                                                        aTTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCg                                         <  1:937314/151‑1 (MQ=255)
                                                                                                           ttATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGcc                                       <  1:450998/150‑1 (MQ=255)
                                                                                                           ttATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCt                                      >  2:743321/1‑151 (MQ=255)
                                                                                                           ttATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCt                                      >  1:175553/1‑151 (MQ=255)
                                                                                                           ttATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCt                                      >  2:194818/1‑151 (MQ=255)
                                                                                                             ttCGGATAACCAACCAAGAAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCTATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGACTGAATTAACCCGGAGTTACCCGCCTg                                     >  1:1488860/2‑150 (MQ=255)
                                                                                                             aTCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTg                                     >  4:530402/1‑150 (MQ=255)
                                                                                                             aTCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTg                                     <  2:1663385/150‑1 (MQ=255)
                                                                                                             aTCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTg                                     >  2:1023870/1‑150 (MQ=255)
                                                                                                             aTCGGATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGa                                    <  2:1317066/151‑1 (MQ=255)
                                                                                                                ggATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATNCNTNNNTTGGAATTGCTTTGTTAAACNATNNNNNNNGCACTAAATNNCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCa                                 <  2:810397/151‑1 (MQ=255)
                                                                                                                ggATAACCAACCAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCa                                 <  1:879278/151‑1 (MQ=255)
                                                                                                                  aTAACCAACCACGGAACGGAATGGTCATAAAGTCGAGAATCGAACTTATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACTTCTCCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGTGTTACCCGCCTGACCaa                                >  1:159329/1‑150 (MQ=255)
                                                                                                                    aaccaaccAAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAAc                             <  3:560870/151‑1 (MQ=255)
                                                                                                                     accaaccaAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACg                            <  2:1330406/151‑1 (MQ=255)
                                                                                                                     accaaccaAGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACg                            <  1:650892/151‑1 (MQ=255)
                                                                                                                       caaccaaGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGc                           >  1:1042813/1‑150 (MQ=255)
                                                                                                                          ccaaGGAACGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGcc                       <  3:218817/151‑1 (MQ=255)
                                                                                                                                  cGGAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgcc               >  2:746887/1‑151 (MQ=255)
                                                                                                                                   ggAATGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgcca              <  2:1291332/151‑1 (MQ=255)
                                                                                                                                     aaTGGTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgccagc            <  4:411362/151‑1 (MQ=255)
                                                                                                                                     aaTGGTAATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTGTGTTAAACAATAACGTCTGCACAAAATAGCCTGCAAGGAAAGTCTGAATTAACCCCGAGTTACCCGCCTGACCAAAACGCAGCCCCGTTgccag             <  1:358225/150‑1 (MQ=255)
                                                                                                                                        ggTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTgccagcca          <  1:1412227/150‑1 (MQ=255)
                                                                                                                                         gTCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGa        <  2:1310569/151‑1 (MQ=255)
                                                                                                                                          tCATAAAGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGa        >  1:1573713/1‑150 (MQ=255)
                                                                                                                                              aaaGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGa    <  1:745089/150‑1 (MQ=255)
                                                                                                                                              aaaGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAt   >  2:626085/1‑151 (MQ=255)
                                                                                                                                               aaGTCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGAtt  <  2:699867/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAATGGTGCTCGGGGCATAGCTTTTATCTACAATGATAGAAAGCATTGGCCATGCCAGCACAATCGCGGAGGTATTCATAATAATATACGGGATCCGGCGACCAATTTTATCGGATAACCAACCAAGGAACGGAATGGTCATAAAGCCGAGAATCGAACTGATCATCAATGCATCTGTTGGAATTGCTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATT  >  NC_000913/1631355‑1631648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: