BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,590	T→C	V298A (GTT→GCT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,590	0	T	C	100.0%	501.1 / NA	132	V298A (GTT→GCT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (72/60); total (72/60)

Only 100 of 132 total aligned reads displayed.

TAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACA  >  NC_000913/1756443‑1756736

tAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTg                                                                                                                                                  <  1:446488/150‑1 (MQ=255)

tAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGc                                                                                                                                                 >  1:324236/1‑151 (MQ=255)

     aTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACg                                                                                                                                            >  1:118688/1‑151 (MQ=255)

      tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGc                                                                                                                                           >  2:419165/1‑151 (MQ=255)

       cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGc                                                                                                                                           <  2:980326/150‑1 (MQ=255)

         cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCAt                                                                                                                                        >  2:1179448/1‑151 (MQ=255)

         cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCAt                                                                                                                                        <  3:349172/151‑1 (MQ=255)

         cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCAACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCAt                                                                                                                                        <  2:821236/151‑1 (MQ=255)

             agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTc                                                                                                                                    >  1:1703439/1‑151 (MQ=255)

               aagaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCg                                                                                                                                   >  1:1064044/1‑150 (MQ=255)

               aagaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCg                                                                                                                                   >  4:19635/1‑150 (MQ=255)

                agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGAc                                                                                                                                 >  1:1524622/1‑151 (MQ=255)

                 gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACg                                                                                                                                >  2:71738/1‑151 (MQ=255)

                   agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGt                                                                                                                              >  2:725763/1‑151 (MQ=255)

                    gTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTa                                                                                                                             <  1:1466745/151‑1 (MQ=255)

                           tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGa                                                                                                                         <  2:770512/148‑1 (MQ=255)

                           tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGa                                                                                                                         <  1:999339/148‑1 (MQ=255)

                           tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGa                                                                                                                         <  1:478507/148‑1 (MQ=255)

                           tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACg                                                                                                                       <  1:497469/150‑1 (MQ=255)

                            cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGc                                                                                                                      <  4:290163/150‑1 (MQ=255)

                            cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGc                                                                                                                      <  1:366381/150‑1 (MQ=255)

                            cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCa                                                                                                                     <  2:564980/151‑1 (MQ=255)

                              cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAg                                                                                                                    >  3:170930/1‑150 (MQ=255)

                               ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                                                                                                                  <  1:1620661/151‑1 (MQ=255)

                               ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                                                                                                                  >  1:478782/1‑151 (MQ=255)

                                   aagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGc                                                                                                              >  1:1041486/1‑151 (MQ=255)

                                           gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGt                                                                                                      <  3:63075/151‑1 (MQ=255)

                                              cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa                                                                                                    <  2:1589306/150‑1 (MQ=255)

                                               gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt                                                                                                  >  4:212572/1‑151 (MQ=255)

                                                aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCAACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGGCTCGCGCAGAAGCCGGTGACGCTGCAAACGCCTTCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt                                                                                                  <  4:325816/150‑1 (MQ=255)

                                                  aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg                                                                                               >  2:843716/1‑151 (MQ=255)

                                                   aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc                                                                                              <  2:585608/151‑1 (MQ=255)

                                                    aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGc                                                                                              >  1:454199/1‑150 (MQ=255)

                                                    aTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa                                                                                             <  1:1133193/151‑1 (MQ=255)

                                                     tGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa                                                                                             >  2:1082605/1‑150 (MQ=255)

                                                      gTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaa                                                                                            <  1:1294574/150‑1 (MQ=255)

                                                       tATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa                                                                                           >  1:494118/1‑150 (MQ=255)

                                                        aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaaa                                                                                          <  1:97446/150‑1 (MQ=255)

                                                        aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa                                                                                           >  2:1058053/1‑149 (MQ=255)

                                                        aTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaa                                                                                            <  2:1639111/148‑1 (MQ=255)

                                                           cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGt                                                                                       >  2:484710/1‑150 (MQ=255)

                                                           cGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTa                                                                                      <  1:1489757/151‑1 (MQ=255)

                                                              gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTaaa                                                                                    <  3:421606/150‑1 (MQ=255)

                                                               cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAAt                                                                                   >  4:364305/1‑150 (MQ=255)

                                                               cACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATa                                                                                  <  1:350052/151‑1 (MQ=255)

                                                                aCGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAc                                                                                 >  3:298578/1‑151 (MQ=255)

                                                                aCGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAc                                                                                 >  2:188604/1‑151 (MQ=255)

                                                                  gTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAccc                                                                               <  4:494825/151‑1 (MQ=255)

                                                                   tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAccc                                                                               <  2:1146773/150‑1 (MQ=255)

                                                                    aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAccca                                                                              <  4:170910/150‑2 (MQ=255)

                                                                    aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                             <  1:1040902/151‑1 (MQ=255)

                                                                     aaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCt                                                                            >  1:1605431/1‑151 (MQ=255)

                                                                      aGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCt                                                                            >  2:966546/1‑150 (MQ=255)

                                                                      aGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCt                                                                            <  1:966423/150‑1 (MQ=255)

                                                                      aGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTg                                                                           <  1:705110/151‑1 (MQ=255)

                                                                        tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTgg                                                                          >  2:1326120/1‑150 (MQ=255)

                                                                         cGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGa                                                                         >  1:666538/1‑150 (MQ=255)

                                                                          gTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGa                                                                         >  4:49336/1‑149 (MQ=255)

                                                                           ttATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAg                                                                       <  1:1669268/150‑1 (MQ=255)

                                                                            tATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGc                                                                      <  1:111629/150‑1 (MQ=255)

                                                                            tATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGc                                                                      <  2:650215/150‑1 (MQ=255)

                                                                             aTCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCgg                                                                    >  3:526980/1‑151 (MQ=255)

                                                                                aCTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGttt                                                                 >  2:810653/1‑151 (MQ=255)

                                                                                   gCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCt                                                               >  4:530692/1‑150 (MQ=255)

                                                                                         cAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATgg                                                        <  1:1243809/151‑1 (MQ=255)

                                                                                           gATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCg                                                      <  1:805645/151‑1 (MQ=255)

                                                                                            aTGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCg                                                      >  2:302608/1‑150 (MQ=255)

                                                                                             tGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGAc                                                    >  4:526339/1‑151 (MQ=255)

                                                                                                  gATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCt                                               <  2:454245/151‑1 (MQ=255)

                                                                                                   aTTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTg                                              <  1:1040336/151‑1 (MQ=255)

                                                                                                     tCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCg                                            >  2:1705841/1‑151 (MQ=255)

                                                                                                       cATGATCAAAACCCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGa                                           <  2:999937/150‑1 (MQ=255)

                                                                                                       cATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGaa                                          <  1:1413660/151‑1 (MQ=255)

                                                                                                       cATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGa                                           >  3:324627/1‑150 (MQ=255)

                                                                                                        aTGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc                                         <  2:1570596/151‑1 (MQ=255)

                                                                                                         tGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACg                                        >  1:1329626/1‑151 (MQ=255)

                                                                                                         tGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGAGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACg                                        >  3:77723/1‑151 (MQ=255)

                                                                                                          gATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACg                                        <  2:883332/150‑1 (MQ=255)

                                                                                                          gATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCAGCGAACGt                                       <  2:1404019/151‑1 (MQ=255)

                                                                                                            tCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCGGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTac                                     >  2:956507/1‑151 (MQ=255)

                                                                                                            tCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTac                                     >  4:535212/1‑151 (MQ=255)

                                                                                                            tCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTac                                     >  1:1687582/1‑151 (MQ=255)

                                                                                                              aaaaaCCTACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGAAAATACCCGCTGGCAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccg                                   >  1:1197214/1‑151 (MQ=255)

                                                                                                               aaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccg                                   >  1:81580/1‑150 (MQ=255)

                                                                                                                 aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                >  4:482531/1‑151 (MQ=255)

                                                                                                                   cccACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgaccg                               >  2:1188156/1‑150 (MQ=255)

                                                                                                                   cccACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACcgc                              >  1:1096147/1‑151 (MQ=255)

                                                                                                                    ccACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACcgcg                             >  1:552672/1‑151 (MQ=255)

                                                                                                                      aCGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtg                           <  2:255845/151‑1 (MQ=255)

                                                                                                                       cGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtg                           >  1:1296759/1‑150 (MQ=255)

                                                                                                                         cccGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgatg                        <  2:509995/151‑1 (MQ=255)

                                                                                                                            gACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAc                     >  2:1711436/1‑151 (MQ=255)

                                                                                                                             aCTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAc                     >  1:88658/1‑150 (MQ=255)

                                                                                                                                cgcgCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAg                   <  2:94169/149‑1 (MQ=255)

                                                                                                                                      gAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCg           <  2:1527129/151‑1 (MQ=255)

                                                                                                                                          ccGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGtt       <  2:760125/151‑1 (MQ=255)

                                                                                                                                           cGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc      >  1:719359/1‑151 (MQ=255)

                                                                                                                                               gACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaaca  <  4:127567/151‑1 (MQ=255)

                                                                                                                                                aCGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGt                                       >  3:137298/1‑113 (MQ=255)

                                                                                                                                                aCGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGt                                       <  4:137277/113‑1 (MQ=255)

TAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACA  >  NC_000913/1756443‑1756736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`