Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,929,306 G→A A134A (GCC→GCT)  yebE ← DUF533 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,929,3060GA99.2% 482.5 / ‑5.5 122A134A (GCC→GCT) yebEDUF533 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (72/49);  total (73/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 122 total aligned reads displayed.

TAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTG  >  NC_000913/1929158‑1929452
                                                                                                                                                    |                                                                                                                                                  
tAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGc                                                                                                                                                  >  3:176410/1‑151 (MQ=255)
 aaaaTGGTCAATATCAATAGCCGCTCAACTCAGGAAATTGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTATCCAGGCGTTGTGGATCCCGCGGTTGTTCGATTGCCTTCTTAATGAGTACCCGCCCCTGCTCTTCCACGCCAGCt                                                                                                                                                 <  1:1581155/151‑1 (MQ=255)
   aaTGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTcc                                                                                                                                               >  2:772261/1‑151 (MQ=255)
   aaTGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTcc                                                                                                                                               >  3:56883/1‑151 (MQ=255)
    aTGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTCCACGCCCATGCTATTCCACGCCAGCTcc                                                                                                                                               >  1:1127216/1‑150 (MQ=255)
    aTGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCa                                                                                                                                              <  3:494337/151‑1 (MQ=255)
        tcaatatcaataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGc                                                                                                                                           >  1:1640415/1‑150 (MQ=255)
          aatatcaataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAAt                                                                                                                                        >  3:196215/1‑151 (MQ=255)
             atcaataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTg                                                                                                                                      <  1:417455/150‑1 (MQ=255)
              tcaataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCt                                                                                                                                    >  4:315920/1‑151 (MQ=255)
              tcaataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCt                                                                                                                                    >  3:560528/1‑151 (MQ=255)
                aataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTgg                                                                                                                                  >  2:484639/1‑151 (MQ=255)
                 ataGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGt                                                                                                                                 >  1:1383638/1‑151 (MQ=255)
                  taGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTc                                                                                                                                >  2:623819/1‑151 (MQ=255)
                  taGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTc                                                                                                                                >  4:387831/1‑151 (MQ=255)
                   aGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTc                                                                                                                                <  4:399735/150‑1 (MQ=255)
                   aGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTc                                                                                                                                <  3:473646/150‑1 (MQ=255)
                          cAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCg                                                                                                                        >  2:1090372/1‑151 (MQ=255)
                          cAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCg                                                                                                                        >  2:570006/1‑151 (MQ=255)
                            aCTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGc                                                                                                                       >  1:679512/1‑150 (MQ=255)
                              tCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACg                                                                                                                    >  2:969338/1‑151 (MQ=255)
                              tCAGGAAATAGATTTCCAGCGCCTCTTCTTCATCGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACCCCAGCTCCACGCAATTGCTGGTCGACTGCCGCAc                                                                                                                     >  1:920628/1‑150 (MQ=255)
                               cAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGt                                                                                                                   >  1:258643/1‑151 (MQ=255)
                                aGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGt                                                                                                                   <  1:49314/150‑1 (MQ=255)
                                 ggAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTc                                                                                                                 <  2:614471/151‑1 (MQ=255)
                                  gAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCt                                                                                                                <  3:387851/151‑1 (MQ=255)
                                  gAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCt                                                                                                                >  2:1650751/1‑151 (MQ=255)
                                     aTAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTg                                                                                                             <  1:1314178/151‑1 (MQ=255)
                                       aGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTgg                                                                                                            <  4:18096/150‑1 (MQ=255)
                                       aGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGc                                                                                                           >  2:1245952/1‑151 (MQ=255)
                                        gATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGc                                                                                                           >  2:791109/1‑150 (MQ=255)
                                         aTTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCa                                                                                                          <  4:398223/150‑1 (MQ=255)
                                          tttCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATc                                                                                                        >  2:93370/1‑151 (MQ=255)
                                           ttCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATc                                                                                                        >  3:511549/1‑150 (MQ=255)
                                            tCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCa                                                                                                       >  2:251488/1‑150 (MQ=255)
                                            tCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCa                                                                                                       <  2:1488781/150‑1 (MQ=255)
                                             ccAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCaa                                                                                                      >  1:191574/1‑150 (MQ=255)
                                              cAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAata                                                                                                    <  1:1118442/151‑1 (MQ=255)
                                               aGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAatat                                                                                                   >  2:1120316/1‑151 (MQ=255)
                                                       ttcttcATTGCGGACCCCGGTAGCCAGGTGTTGTGGATCCAGAGGTTGTTCGATTGCCTGCTCAATGAGTACACGACCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTAGATTGCCGCAAGTTCTTTGGCATCAATATGACAATc                                                                                            >  4:112354/1‑150 (MQ=255)
                                                       ttcttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATc                                                                                            >  1:1007005/1‑150 (MQ=255)
                                                        tcttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                             <  4:575475/148‑1 (MQ=255)
                                                         cttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCAt                                                                                             <  3:323164/147‑1 (MQ=255)
                                                          ttcATTGCGGATCCCGGTAGCCCGGCGTGGTGGATCTAGCGGTTGTTCGCTTGCTCGCTTAATGAGTACACGCCCCTCCACTACCACGCCAGCTCCTCGCAATTGCTGGTCTATTGCCGCTGGTTCTGTGGCATCAATATGACCATCACt                                                                                         <  1:100065/150‑1 (MQ=255)
                                                          ttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtt                                                                                        >  2:1358188/1‑151 (MQ=255)
                                                          ttcATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtt                                                                                        >  1:568823/1‑151 (MQ=255)
                                                            cATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtttt                                                                                      >  2:952481/1‑151 (MQ=255)
                                                            cATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtt                                                                                        <  1:1053816/149‑1 (MQ=255)
                                                             aTTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACtttt                                                                                      >  1:1259430/1‑150 (MQ=255)
                                                               tGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAg                                                                                    <  4:257418/150‑1 (MQ=255)
                                                                gCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGc                                                                                   <  1:1072728/150‑1 (MQ=255)
                                                                gCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCg                                                                                  <  2:1198135/151‑1 (MQ=255)
                                                                    aCCCCGGTAGCCAGGCGTTGTGGATCCAGCGGGTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCa                                                                               >  1:1527196/1‑150 (MQ=255)
                                                                           tAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCTGCAAAGAcc                                                                         >  1:781345/1‑149 (MQ=255)
                                                                            aGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAgc                                                                      >  4:241635/1‑151 (MQ=255)
                                                                              ccAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAgcgc                                                                    <  1:1120404/151‑1 (MQ=255)
                                                                              ccAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAgcgc                                                                    >  2:577187/1‑151 (MQ=255)
                                                                               cAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAgcgc                                                                    >  2:334073/1‑150 (MQ=255)
                                                                                      tgtgGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATc                                                             >  2:1470265/1‑150 (MQ=255)
                                                                                         ggATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAAc                                                         <  3:315939/151‑1 (MQ=255)
                                                                                         ggATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGACCCTGCTCTTCAACGCCAGCTCCAAGCAATTGCTGGTCGATTGCCGCACGTTCTTCGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAAc                                                         >  1:1381582/1‑151 (MQ=255)
                                                                                           aTCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGt                                                       <  4:110870/151‑1 (MQ=255)
                                                                                              cAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGc                                                     >  4:516477/1‑150 (MQ=255)
                                                                                              cAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCt                                                    >  2:1644815/1‑151 (MQ=255)
                                                                                               aGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTg                                                   >  3:382424/1‑151 (MQ=255)
                                                                                                 cGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGt                                                  >  1:1700913/1‑150 (MQ=255)
                                                                                                   gttgttCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACg                                               >  2:892113/1‑151 (MQ=255)
                                                                                                      gttCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCt                                             <  1:1311579/150‑1 (MQ=255)
                                                                                                      gttCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTc                                            <  4:323068/151‑1 (MQ=255)
                                                                                                         cGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATc                                         >  2:831839/1‑151 (MQ=255)
                                                                                                             tGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgc                                     >  2:1328360/1‑151 (MQ=255)
                                                                                                             tGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgc                                     >  3:327768/1‑151 (MQ=255)
                                                                                                             tGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAg                                      <  2:1601876/150‑1 (MQ=255)
                                                                                                              gCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcg                                    <  2:910170/151‑1 (MQ=255)
                                                                                                                cTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcgg                                   >  1:1549395/1‑150 (MQ=255)
                                                                                                                  gCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggcg                                 <  1:1293190/150‑1 (MQ=255)
                                                                                                                  gCTCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAgcggc                                  <  1:865904/149‑1 (MQ=255)
                                                                                                                    tCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTAc                              >  2:807539/1‑151 (MQ=255)
                                                                                                                    tCAATGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTAc                              >  1:33579/1‑151 (MQ=255)
                                                                                                                      aaTGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACtt                            >  3:127460/1‑151 (MQ=255)
                                                                                                                      aaTGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACtt                            >  2:1411754/1‑151 (MQ=255)
                                                                                                                      aaTGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACtt                            <  1:547204/151‑1 (MQ=255)
                                                                                                                       aTGAGTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACttt                           >  2:597577/1‑151 (MQ=255)
                                                                                                                           gTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcgc                       >  4:363360/1‑151 (MQ=255)
                                                                                                                           gTACACGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcgc                       >  4:362958/1‑151 (MQ=255)
                                                                                                                             acacGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcgcgc                     >  3:487889/1‑151 (MQ=255)
                                                                                                                             acacGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcgcgc                     >  2:411300/1‑151 (MQ=255)
                                                                                                                             acacGCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTgcgcgc                     <  1:772156/151‑1 (MQ=255)
                                                                                                                                 gCCCCTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCaa                  >  1:1054350/1‑150 (MQ=255)
                                                                                                                                   cccTGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACt               >  1:133945/1‑151 (MQ=255)
                                                                                                                                      tGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCg            >  2:941862/1‑151 (MQ=255)
                                                                                                                                      tGCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCg            >  4:62058/1‑151 (MQ=255)
                                                                                                                                       gCTCTTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCg            <  2:258685/150‑1 (MQ=255)
                                                                                                                                          ctTCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTc        >  1:1652301/1‑151 (MQ=255)
                                                                                                                                            tCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACTTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGTCAAACTGCGGTTc        <  2:1383789/149‑1 (MQ=255)
                                                                                                                                            tCCACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCg       <  1:851295/150‑1 (MQ=255)
                                                                                                                                             ccACGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGt      <  1:484562/150‑1 (MQ=255)
                                                                                                                                                cGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCt   <  1:1406564/150‑1 (MQ=255)
                                                                                                                                                cGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTg  <  1:1090240/151‑1 (MQ=255)
                                                                                                                                                cGCCAGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTg  <  4:518839/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TAAAATGGTCAATATCAATAGCCGCGCAACTCAGGAAATAGATTTCCAGCGCCTCTTCTTCATTGCGGACCCCGGTAGCCAGGCGTTGTGGATCCAGCGGTTGTTCGATTGCCTGCTCAATGAGTACACGCCCCTGCTCTTCCACGCCGGCTCCACGCAATTGCTGGTCGATTGCCGCACGTTCTTTGGCATCAATATGACCATCACTTTTAGCGGCAAAGACCAGCGCAAGGATCAAACGTGCTGTACGCTCATCCAGCGGCGTACTTTGCGCGCCAAACTGCGGTTCGTCCTG  >  NC_000913/1929158‑1929452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑