Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,974,562 T→C T255A (ACG→GCG)  cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,974,5620TC100.0% 465.1 / NA 129T255A (ACG→GCG) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (70/59);  total (70/59)

Only 100 of 144 total aligned reads displayed.

ATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974414‑1974709
                                                                                                                                                    |                                                                                                                                                   
aTCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGctt                                                                                                                                                   >  1:604008/1‑151 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGTTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGTGCGTAGttttt                                                                                                                                                 >  1:596219/1‑151 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGcttt                                                                                                                                                  <  3:37527/150‑1 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGcttt                                                                                                                                                  <  1:506966/150‑1 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGcttt                                                                                                                                                  <  1:505825/150‑1 (MQ=255)
  cAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGcttt                                                                                                                                                  <  1:37509/150‑1 (MQ=255)
   aaaTCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGcttttt                                                                                                                                                >  1:353533/1‑151 (MQ=255)
    aaTCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTc                                                                                                                                               >  1:146576/1‑151 (MQ=255)
      tCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTccc                                                                                                                                             >  1:226978/1‑151 (MQ=255)
       cACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCg                                                                                                                                            >  2:49295/1‑151 (MQ=255)
           aTGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTcc                                                                                                                                        >  2:1015627/1‑151 (MQ=255)
            tGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGGGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTc                                                                                                                                         >  1:1560305/1‑149 (MQ=255)
             gATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCa                                                                                                                                       <  1:1413372/150‑1 (MQ=255)
             gATTAACCGGGTCCAGTTCGCCGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAc                                                                                                                                      <  2:77746/151‑1 (MQ=255)
              aTTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcg                                                                                                                                     >  2:711485/1‑151 (MQ=255)
              aTTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcg                                                                                                                                     >  1:251022/1‑151 (MQ=255)
               ttAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcgc                                                                                                                                    >  1:1581144/1‑151 (MQ=255)
                   ccGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGcc                                                                                                                                <  4:328172/151‑1 (MQ=255)
                    cGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGcct                                                                                                                               <  1:978564/151‑2 (MQ=255)
                     gggTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCgg                                                                                                                              <  2:1087029/151‑1 (MQ=255)
                     gggTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCgg                                                                                                                              >  2:471842/1‑151 (MQ=255)
                      ggTCCAGTTCGCTGGAACGCTGGGCAAGAATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGACGCTTTTTCCCGCTCCACGCGGCCgg                                                                                                                              >  2:1009081/1‑150 (MQ=255)
                           aGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGgcg                                                                                                                        <  1:829263/151‑1 (MQ=255)
                             ttCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGgcgc                                                                                                                       >  2:985922/1‑150 (MQ=255)
                              tCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCt                                                                                                                      <  1:848566/150‑1 (MQ=255)
                                         cTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGcc                                                                                                          >  1:1451356/1‑151 (MQ=255)
                                           gggCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGcc                                                                                                          <  2:1633292/149‑1 (MQ=255)
                                           gggCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGcc                                                                                                          >  1:1268500/1‑149 (MQ=255)
                                             gCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGtt                                                                                                      <  1:646996/151‑1 (MQ=255)
                                              cAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGttt                                                                                                     <  2:402488/151‑1 (MQ=255)
                                                  cATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGc                                                                                                 <  2:696706/151‑1 (MQ=255)
                                                   aTGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGc                                                                                                 >  3:105908/1‑150 (MQ=255)
                                                   aTGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGc                                                                                                 >  1:179243/1‑150 (MQ=255)
                                                    tGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCAc                                                                                               >  1:1290435/1‑151 (MQ=255)
                                                      gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             >  1:1371188/1‑151 (MQ=255)
                                                      gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             >  1:1575360/1‑151 (MQ=255)
                                                       aCTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtgg                                                                                            <  3:18916/151‑1 (MQ=255)
                                                          gggTGATAACCCGCGCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggtgg                                                                                         <  2:410159/151‑1 (MQ=255)
                                                          gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggt                                                                                           <  4:330652/149‑1 (MQ=255)
                                                           ggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggg                                                                                        <  2:1113618/151‑1 (MQ=255)
                                                               aTAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggggtgg                                                                                    <  2:880977/151‑1 (MQ=255)
                                                               aTAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTggtggggtgg                                                                                    >  1:240758/1‑151 (MQ=255)
                                                                  aCCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGat                                                                                  <  1:1461537/150‑1 (MQ=255)
                                                                   ccAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGatat                                                                                <  2:195401/151‑1 (MQ=255)
                                                                   ccAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGatat                                                                                >  1:1635269/1‑151 (MQ=255)
                                                                     aGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATAttt                                                                              <  1:1266007/151‑1 (MQ=255)
                                                                      gCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATAtttt                                                                             <  1:1155142/151‑1 (MQ=255)
                                                                         cGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTg                                                                           <  3:366586/150‑1 (MQ=255)
                                                                         cGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTg                                                                           <  3:495208/150‑1 (MQ=255)
                                                                           ccGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCg                                                                        >  1:1589599/1‑151 (MQ=255)
                                                                            cgacgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCga                                                                       <  2:1702327/151‑1 (MQ=255)
                                                                            cgacgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCg                                                                        >  4:3534/1‑150 (MQ=255)
                                                                             gacgaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCg                                                                        >  1:226680/1‑149 (MQ=255)
                                                                                gaGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACt                                                                   <  3:235429/151‑1 (MQ=255)
                                                                                 aGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACtt                                                                  >  1:785967/1‑151 (MQ=255)
                                                                                 aGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACtt                                                                  >  2:540181/1‑151 (MQ=255)
                                                                                    ttaattaaTTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCt                                                                >  2:141108/1‑150 (MQ=255)
                                                                                         taattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGtt                                                          >  2:455431/1‑151 (MQ=255)
                                                                                         taattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGtt                                                          >  2:175791/1‑151 (MQ=255)
                                                                                          aattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGttt                                                         >  1:1140505/1‑151 (MQ=255)
                                                                                          aattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGttt                                                         >  1:540467/1‑151 (MQ=255)
                                                                                             tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                       <  2:1617577/150‑1 (MQ=255)
                                                                                             tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                       <  2:353584/150‑1 (MQ=255)
                                                                                              gATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaa                                                      <  2:28631/150‑1 (MQ=255)
                                                                                               aTCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaa                                                      >  2:962916/1‑149 (MQ=255)
                                                                                               aTCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaa                                                      >  1:865683/1‑149 (MQ=255)
                                                                                               aTCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCaa                                                      >  1:193694/1‑149 (MQ=255)
                                                                                               aTCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAg                                                    <  2:829240/151‑1 (MQ=255)
                                                                                                tCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAgg                                                   >  4:385156/1‑151 (MQ=255)
                                                                                                 cAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCTCGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGt                                                  <  2:1435593/151‑1 (MQ=255)
                                                                                                  aaCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGt                                                  >  1:258706/1‑150 (MQ=255)
                                                                                                   aCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 >  2:12963/1‑150 (MQ=255)
                                                                                                   aCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 >  2:529781/1‑150 (MQ=255)
                                                                                                       tttCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCt                                             <  3:591377/150‑1 (MQ=255)
                                                                                                        ttCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTg                                            >  1:1021241/1‑150 (MQ=255)
                                                                                                           tACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCg                                        >  4:526960/1‑151 (MQ=255)
                                                                                                            aCCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATCTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCgg                                       >  3:531035/1‑151 (MQ=255)
                                                                                                             ccGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc                                      <  3:190342/151‑1 (MQ=255)
                                                                                                              cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTaa                                                <  4:355267/140‑1 (MQ=255)
                                                                                                               gCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCtt                                    <  2:179271/151‑1 (MQ=255)
                                                                                                                cTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCtt                                    >  2:543919/1‑150 (MQ=255)
                                                                                                                 tACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc                                   >  4:258767/1‑150 (MQ=255)
                                                                                                                  acacGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc                                   <  2:1542605/149‑1 (MQ=255)
                                                                                                                  acacGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTc                                   <  4:379620/149‑1 (MQ=255)
                                                                                                                  acacGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCa                                  >  1:1605731/1‑150 (MQ=255)
                                                                                                                     cGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATca                              <  2:1586260/151‑1 (MQ=255)
                                                                                                                     cGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATca                              <  1:900917/151‑1 (MQ=255)
                                                                                                                      ggATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATcac                             >  2:927610/1‑151 (MQ=255)
                                                                                                                      ggATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATcac                             >  3:51091/1‑151 (MQ=255)
                                                                                                                        aTGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACaa                           <  2:328953/151‑1 (MQ=255)
                                                                                                                             ggtggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACa                       <  1:1059729/150‑1 (MQ=255)
                                                                                                                              gtgtATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGGGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACaaaa                         >  1:700504/1‑147 (MQ=255)
                                                                                                                              gtggATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACaga                     <  2:1600983/151‑1 (MQ=255)
                                                                                                                                  aTTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTAc                 <  2:27076/151‑1 (MQ=255)
                                                                                                                                     cATTGCTGCGCGTCGCTTTTTCCCGCGCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGGTCGGCTTCAATCACAAAACAGAGTACCGc              <  1:1008952/151‑1 (MQ=255)
                                                                                                                                      aTTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCt             <  1:677162/151‑1 (MQ=255)
                                                                                                                                        tgctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtg            <  4:566758/150‑1 (MQ=255)
                                                                                                                                            gcgcGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGAt        <  1:1668141/150‑1 (MQ=255)
                                                                                                                                              gcgTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTc     <  2:604092/151‑1 (MQ=255)
                                                                                                                                                 tcgcTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATc  >  1:1192802/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCAAATCACCATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974414‑1974709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑