Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,000,046 G→A R114C (CGC→TGC)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,000,0460GA99.3% 529.5 / NA 135R114C (CGC→TGC) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (75/59);  minor base T (0/1);  total (75/60)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ACTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC  >  NC_000913/1999899‑2000192
                                                                                                                                                   |                                                                                                                                                  
aCTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACtc                                                                                                                                                 <  4:397310/151‑1 (MQ=255)
 ctctTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACtc                                                                                                                                                 <  1:1679103/150‑1 (MQ=255)
  tctTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCCCCTGAATACCAGAAATGGTGTACGGGGTTGGGACATCGTATTTTTTCTTGCACtca                                                                                                                                                >  1:1027837/1‑150 (MQ=255)
      cATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACtcatc                                                                                                                                              >  1:1416804/1‑148 (MQ=255)
       aTAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGaa                                                                                                                                          <  1:915070/151‑1 (MQ=255)
        tAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGaa                                                                                                                                          >  3:160738/1‑150 (MQ=255)
         aGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAAt                                                                                                                                        >  2:1350559/1‑151 (MQ=255)
             ggTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGt                                                                                                                                     >  2:5321/1‑150 (MQ=255)
             ggTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTg                                                                                                                                    <  1:1273442/151‑1 (MQ=255)
               tGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGAc                                                                                                                                  >  2:436998/1‑151 (MQ=255)
                gCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGAcc                                                                                                                                 >  4:471719/1‑151 (MQ=255)
                  ccAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTg                                                                                                                               <  2:903002/151‑1 (MQ=255)
                   cAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGa                                                                                                                              <  2:419831/151‑1 (MQ=255)
                    tgaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGGTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGAt                                                                                                                             >  4:155295/2‑151 (MQ=255)
                    aGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGAt                                                                                                                             >  2:231035/1‑151 (MQ=255)
                      accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTa                                                                                                                           <  3:80825/151‑1 (MQ=255)
                        cgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAAt                                                                                                                         >  3:463672/1‑151 (MQ=255)
                           cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATca                                                                                                                       <  1:430840/150‑1 (MQ=255)
                             cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATcacc                                                                                                                     >  1:47899/1‑150 (MQ=255)
                              ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATcaccac                                                                                                                   >  2:897852/1‑151 (MQ=255)
                                aCTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTTACCAGCGCCTGAATACCAGAAATGGTGTAAGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                  >  1:180841/1‑150 (MQ=255)
                                   tttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATc                                                                                                                <  1:1103562/149‑1 (MQ=255)
                                   tttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCa                                                                                                               >  4:235459/1‑150 (MQ=255)
                                   tttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCa                                                                                                               <  2:388494/150‑1 (MQ=255)
                                    ttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                             >  4:64738/1‑151 (MQ=255)
                                     tttGCCTTTCAGATCATCGGCTGTTTTAATGGAGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                             >  1:1323399/1‑150 (MQ=255)
                                     tttGCCTTTCAGATCATCGGCTGCTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                             >  3:189613/1‑150 (MQ=255)
                                      ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATa                                                                                                            <  2:1321956/150‑1 (MQ=255)
                                        gCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTGCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                         <  2:1252569/151‑1 (MQ=255)
                                         ccTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                        <  2:522753/151‑1 (MQ=255)
                                         ccTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                        <  2:1447466/151‑1 (MQ=255)
                                         ccTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAACGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                        >  4:504159/1‑151 (MQ=255)
                                            ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGttt                                                                                                       <  1:1729170/149‑1 (MQ=255)
                                            ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa                                                                                                      <  4:304880/150‑1 (MQ=255)
                                             tCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGttt                                                                                                       >  1:708405/1‑148 (MQ=255)
                                               aGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGt                                                                                                  <  2:324229/151‑1 (MQ=255)
                                               aGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGt                                                                                                  >  4:10655/1‑151 (MQ=255)
                                                gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                 <  2:872284/151‑1 (MQ=255)
                                                  tcatcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                                >  1:709125/1‑150 (MQ=255)
                                                      aggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc                                                                                             >  1:1665500/2‑149 (MQ=255)
                                                       ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGAc                                                                                          <  2:225833/151‑1 (MQ=255)
                                                          tGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGcc                                                                                       >  2:1213714/1‑151 (MQ=255)
                                                             tttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGc                                                                                    >  1:91786/1‑151 (MQ=255)
                                                               tAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                   <  2:139064/150‑1 (MQ=255)
                                                                  tGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAcc                                                                               >  2:788905/1‑151 (MQ=255)
                                                                   ggTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCg                                                                              <  4:461769/151‑1 (MQ=255)
                                                                    gTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGt                                                                             >  3:482491/1‑151 (MQ=255)
                                                                       ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                          >  3:251822/1‑151 (MQ=255)
                                                                       ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                          >  1:1669426/1‑151 (MQ=255)
                                                                       ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                          >  1:463215/1‑151 (MQ=255)
                                                                        cTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                          >  1:62384/1‑150 (MQ=255)
                                                                         ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAt                                                                        >  2:1180807/1‑151 (MQ=255)
                                                                           cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAttt                                                                      >  1:840520/1‑151 (MQ=255)
                                                                           cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAtt                                                                       <  1:75531/150‑1 (MQ=255)
                                                                               aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGt                                                                   <  2:676607/150‑1 (MQ=255)
                                                                                 cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCgg                                                                >  2:654120/1‑151 (MQ=255)
                                                                                 cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCgg                                                                >  1:126678/1‑151 (MQ=255)
                                                                                   tttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                              >  2:1385627/1‑151 (MQ=255)
                                                                                    ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGttt                                                             >  1:1000394/1‑151 (MQ=255)
                                                                                     tttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtttt                                                            >  2:1399926/1‑151 (MQ=255)
                                                                                            aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAc                                                      >  1:930280/1‑150 (MQ=255)
                                                                                                ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTc                                                 <  1:747255/151‑1 (MQ=255)
                                                                                                    aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACg                                             >  3:283212/1‑151 (MQ=255)
                                                                                                      tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGcc                                           >  4:537761/1‑151 (MQ=255)
                                                                                                        ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                         <  1:298758/151‑1 (MQ=255)
                                                                                                        ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCa                                          <  4:160717/150‑1 (MQ=255)
                                                                                                         cAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCa                                          >  2:35942/1‑149 (MQ=255)
                                                                                                           gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCa                                          >  1:956060/1‑147 (MQ=255)
                                                                                                                 gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                <  1:1720694/151‑1 (MQ=255)
                                                                                                                 gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                >  4:437319/1‑151 (MQ=255)
                                                                                                                 gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                <  2:380702/151‑1 (MQ=255)
                                                                                                                     aCGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCa                             <  2:1479242/150‑1 (MQ=255)
                                                                                                                     aCGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCa                             <  1:1482774/150‑1 (MQ=255)
                                                                                                                     aCGGGGTTGAGAAATCGTATTTTTTCTTGCACTCACCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCa                             <  1:88368/150‑1 (MQ=255)
                                                                                                                      cGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                           <  2:820274/151‑1 (MQ=255)
                                                                                                                       ggggTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAGCGCCAAGATGTTTTGCCAGCt                          <  2:1690046/151‑1 (MQ=255)
                                                                                                                        gggTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCt                          >  1:274646/1‑150 (MQ=255)
                                                                                                                         ggTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                        >  1:1585650/1‑151 (MQ=255)
                                                                                                                          gTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                       <  2:842448/151‑1 (MQ=255)
                                                                                                                            tGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                         <  2:835373/147‑1 (MQ=255)
                                                                                                                            tGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                         >  1:835266/1‑147 (MQ=255)
                                                                                                                             gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCTCCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTgg                     <  2:1274467/150‑1 (MQ=255)
                                                                                                                             gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTggg                    >  2:662623/1‑151 (MQ=255)
                                                                                                                             gagaAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTg                      <  2:1054951/149‑1 (MQ=255)
                                                                                                                                aaaTCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaa                 <  1:138642/151‑1 (MQ=255)
                                                                                                                                aaaTCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaa                 <  2:775500/151‑1 (MQ=255)
                                                                                                                                 aaTCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaa                 >  1:1584887/1‑150 (MQ=255)
                                                                                                                                  aTCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAt               >  4:64393/1‑151 (MQ=255)
                                                                                                                                    cGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt              <  1:938968/150‑1 (MQ=255)
                                                                                                                                    cGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt              <  1:1701192/150‑1 (MQ=255)
                                                                                                                                     gTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTcc            >  4:186020/1‑151 (MQ=255)
                                                                                                                                       aTTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCa           <  3:500361/150‑1 (MQ=255)
                                                                                                                                         tttttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGTGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACt         <  3:10656/150‑1 (MQ=255)
                                                                                                                                         tttttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt        >  3:573388/1‑151 (MQ=255)
                                                                                                                                         tttttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACt         <  2:1296744/150‑1 (MQ=255)
                                                                                                                                          ttttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt        <  1:844901/150‑1 (MQ=255)
                                                                                                                                           tttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTc       >  4:362683/1‑150 (MQ=255)
                                                                                                                                            ttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCa      >  2:1304695/1‑150 (MQ=255)
                                                                                                                                             tCTTGAACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaa     >  1:777970/1‑150 (MQ=255)
                                                                                                                                                tGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaaa   <  2:795855/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC  >  NC_000913/1999899‑2000192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: