Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,117,002 T→C T42T (ACA→ACG wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,117,0020TC99.2% 475.9 / NA 128T42T (ACA→ACGwcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (48/79);  minor base G (1/0);  total (49/79)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCA  >  NC_000913/2116854‑2117148
                                                                                                                                                    |                                                                                                                                                  
 cgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgtg                                                                                                                                                 <  2:1016424/151‑1 (MQ=255)
 cgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgtg                                                                                                                                                 >  4:567922/1‑151 (MQ=255)
  gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTgcgtgt                                                                                                                                                >  2:775511/1‑151 (MQ=255)
       ccAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCt                                                                                                                                           <  1:1322635/151‑1 (MQ=255)
        cAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGTCGCGTGGGTGTTTTGCGTGTCGCCtt                                                                                                                                          >  1:768826/1‑151 (MQ=255)
          ggTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCtttt                                                                                                                                        <  2:298539/151‑1 (MQ=255)
             aTTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGc                                                                                                                                     <  3:383932/151‑1 (MQ=255)
              tttttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGc                                                                                                                                     >  3:391723/1‑150 (MQ=255)
               ttttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCa                                                                                                                                    <  2:585099/150‑1 (MQ=255)
               ttttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCa                                                                                                                                    <  1:325735/150‑1 (MQ=255)
               ttttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCa                                                                                                                                    <  2:1335487/150‑1 (MQ=255)
                tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAgc                                                                                                                                  >  4:59911/1‑151 (MQ=255)
                 ttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAgc                                                                                                                                  <  4:138372/150‑1 (MQ=255)
                    cGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGTAGCGCga                                                                                                                              <  2:1461702/151‑1 (MQ=255)
                    cGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGcgcg                                                                                                                               <  2:1018433/150‑1 (MQ=255)
                    cGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCga                                                                                                                              <  1:1320948/151‑1 (MQ=255)
                      aTGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCgaga                                                                                                                            <  1:143226/151‑1 (MQ=255)
                        gAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAt                                                                                                                          <  2:1432087/151‑1 (MQ=255)
                        gAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAt                                                                                                                          <  4:296272/151‑1 (MQ=255)
                         aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtc                                                                                                                         <  3:587163/151‑1 (MQ=255)
                         aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtc                                                                                                                         <  1:440420/151‑1 (MQ=255)
                           agCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtctc                                                                                                                       >  4:270364/2‑151 (MQ=255)
                            gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtctc                                                                                                                       <  4:152939/150‑1 (MQ=255)
                            gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATctct                                                                                                                      <  2:739900/151‑1 (MQ=255)
                             ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATctct                                                                                                                      >  2:1230121/1‑150 (MQ=255)
                              cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                                    >  1:1444055/1‑151 (MQ=255)
                              cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                                    >  1:605464/1‑151 (MQ=255)
                              cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                                    >  2:1501543/1‑151 (MQ=255)
                               gcgcAAGGTCTGGCTGGCTCGGGGGCGCAGGTTCGCCACTTTGCCCGTAGGTTCGTCCGGGAACCAGCGGGGTCTGGCAGCAAGGGGGGAATTCGGACATGGAGCGGGGGTGGGAGGGGGGTAGCCATTTTGCAGCGCGAGAAGCTCTAcc                                                                                                                   >  2:816872/1‑151 (MQ=255)
                                cgcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCt                                                                                                                  <  4:62072/151‑1 (MQ=255)
                                   aaGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCa                                                                                                                >  4:414797/1‑150 (MQ=255)
                                     ggTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAAt                                                                                                             <  3:548592/151‑1 (MQ=255)
                                          ggctggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATcc                                                                                                           <  1:467208/148‑1 (MQ=255)
                                           gctggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGGTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCata                                                                                                       <  1:1105712/151‑1 (MQ=255)
                                             tggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATc                                                                                                     >  2:965288/1‑151 (MQ=255)
                                                ctCGGTGGCGCCGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCACGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAAt                                                                                                  >  4:489119/1‑151 (MQ=255)
                                                ctCGGTGGCGCCGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAAt                                                                                                  >  4:259324/1‑151 (MQ=255)
                                                  cGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATaa                                                                                                <  2:902011/151‑1 (MQ=255)
                                                    gTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAATTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAAc                                                                                              <  2:294699/151‑1 (MQ=255)
                                                      ggCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAAcg                                                                                             <  2:587355/150‑1 (MQ=255)
                                                           aGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTa                                                                                        >  2:40979/1‑150 (MQ=255)
                                                              ttCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAAttt                                                                                    >  1:1609673/1‑151 (MQ=255)
                                                                  ccACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGtt                                                                                >  2:1345943/1‑151 (MQ=255)
                                                                   cACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGtt                                                                                >  1:1410893/1‑150 (MQ=255)
                                                                    aCTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTaa                                                                              <  3:151912/151‑1 (MQ=255)
                                                                    aCTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTaa                                                                              <  1:428662/151‑1 (MQ=255)
                                                                       ttGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAgg                                                                            >  4:574336/1‑150 (MQ=255)
                                                                          cccGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGa                                                                        <  2:1117198/151‑1 (MQ=255)
                                                                            cGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAg                                                                      <  1:827812/151‑1 (MQ=255)
                                                                            cGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAg                                                                      <  2:1659421/151‑1 (MQ=255)
                                                                            cGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAg                                                                      <  2:927395/151‑1 (MQ=255)
                                                                               aGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGtt                                                                   >  2:1226728/1‑151 (MQ=255)
                                                                                ggTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGttt                                                                  <  2:254481/151‑1 (MQ=255)
                                                                                 ggtCGTCCTGTAACCAGCGGGTTCTTGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGtt                                                                   <  1:196460/147‑1 (MQ=255)
                                                                                 gTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTc                                                                 <  2:1043992/151‑1 (MQ=255)
                                                                                   tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTg                                                               >  1:1066196/1‑151 (MQ=255)
                                                                                          gTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACa                                                         <  1:827020/150‑1 (MQ=255)
                                                                                              ccAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCggg                                                    >  1:1060694/1‑151 (MQ=255)
                                                                                                aGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGaa                                                  <  3:468839/151‑1 (MQ=255)
                                                                                                aGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGa                                                   >  2:1526138/1‑150 (MQ=255)
                                                                                                 gCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAt                                                 <  1:209657/151‑1 (MQ=255)
                                                                                                   gggctgtGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGGCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAttt                                               >  1:479066/7‑151 (MQ=255)
                                                                                                   gggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAttt                                               >  2:1120368/1‑151 (MQ=255)
                                                                                                    ggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  2:1544676/1‑151 (MQ=255)
                                                                                                    ggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  1:1065872/1‑151 (MQ=255)
                                                                                                    ggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  2:1027262/1‑151 (MQ=255)
                                                                                                    ggTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              <  2:194685/151‑1 (MQ=255)
                                                                                                     gTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTc                                              >  2:689292/1‑150 (MQ=255)
                                                                                                     gTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCa                                             <  2:1037589/151‑1 (MQ=255)
                                                                                                         tGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTTAGTa                                          >  3:224605/1‑150 (MQ=255)
                                                                                                          ggCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTa                                          <  1:583107/149‑1 (MQ=255)
                                                                                                           gcagcaAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAAAGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTaaaa                                       <  3:414817/151‑1 (MQ=255)
                                                                                                             agcaAGGTTGTCTTTCGTCCCTGCCTCGTGGGTCTTTTGCGTGTCGCCTTTTTGCTTCGCGCTCGTCTCTACCTCTCCTCCCATCTCCATCAACGCGGTAATTTGGTTAAGGGCGAATGTTTCTGCCGACAGCGGTCCTTTCCTTAAAaag                                     <  1:816765/151‑1 (MQ=255)
                                                                                                             agcaAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaag                                     <  1:55253/151‑1 (MQ=255)
                                                                                                               caAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaagaa                                   <  2:1226705/151‑1 (MQ=255)
                                                                                                                 aGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAaagaa                                   <  2:43635/149‑1 (MQ=255)
                                                                                                                 aGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGc                                 >  2:794448/1‑151 (MQ=255)
                                                                                                                     tGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGAc                             >  2:99594/1‑151 (MQ=255)
                                                                                                                      gTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGAcc                            <  4:331056/151‑1 (MQ=255)
                                                                                                                       tATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCt                           >  1:210522/1‑151 (MQ=255)
                                                                                                                        aTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCtt                          <  1:458045/151‑1 (MQ=255)
                                                                                                                        aTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCtt                          <  2:1056877/151‑1 (MQ=255)
                                                                                                                           tCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCa                        <  3:411904/150‑1 (MQ=255)
                                                                                                                            cGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCAt                       <  2:791810/150‑1 (MQ=255)
                                                                                                                             gTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTGAAAAGAAGCCGACCTTCAttt                     >  3:584479/1‑151 (MQ=255)
                                                                                                                               ccATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    >  1:1520406/1‑150 (MQ=255)
                                                                                                                               ccATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    <  1:1316501/150‑1 (MQ=255)
                                                                                                                               ccATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    >  1:1140726/1‑150 (MQ=255)
                                                                                                                               ccATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTc                    <  2:828394/150‑1 (MQ=255)
                                                                                                                                cATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCAc                  >  2:792352/1‑151 (MQ=255)
                                                                                                                                   gCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCt                <  1:1171371/150‑1 (MQ=255)
                                                                                                                                      gcgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTcccc            >  4:327443/1‑151 (MQ=255)
                                                                                                                                       cgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCg           >  2:949963/1‑151 (MQ=255)
                                                                                                                                       cgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCg           <  1:1579027/151‑1 (MQ=255)
                                                                                                                                          gggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGAt         <  4:170009/150‑1 (MQ=255)
                                                                                                                                          gggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATg        <  2:1376309/151‑1 (MQ=255)
                                                                                                                                           ggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATgc       <  2:1636318/151‑1 (MQ=255)
                                                                                                                                              gtTTTGCGTGTCTCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACACGGTAATTTGGTTAAGTACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCTATGCGCt    <  1:313490/151‑1 (MQ=255)
                                                                                                                                                tttGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTc   >  2:1646598/1‑150 (MQ=255)
                                                                                                                                                 ttGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCa  <  2:406925/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCA  >  NC_000913/2116854‑2117148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: