Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,124,650 G→A A366V (GCG→GTG)  wcaI ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,124,6500GA98.5% 484.0 / ‑3.3 132A366V (GCG→GTG) wcaIputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (63/66);  total (66/66)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 132 total aligned reads displayed.

AGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTG  >  NC_000913/2124502‑2124797
                                                                                                                                                    |                                                                                                                                                   
agagTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCAcc                                                                                                                                                   >  2:1168413/1‑151 (MQ=255)
  agTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTc                                                                                                                                                 <  2:1356561/151‑1 (MQ=255)
  agTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTc                                                                                                                                                 <  2:699582/151‑1 (MQ=255)
   gTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCTTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCg                                                                                                                                                <  4:582641/151‑1 (MQ=255)
     ttCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGAc                                                                                                                                              >  2:420473/1‑151 (MQ=255)
       cGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGAcc                                                                                                                                             <  1:56787/150‑1 (MQ=255)
         aCTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGAt                                                                                                                                          >  3:495616/1‑151 (MQ=255)
         aCTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGAt                                                                                                                                          <  2:2208/151‑1 (MQ=255)
          cTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGAtt                                                                                                                                         >  1:1459019/1‑151 (MQ=255)
            gcgcCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGAtt                                                                                                                                         <  1:512140/149‑1 (MQ=255)
            gcgcCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGAtt                                                                                                                                         <  1:1530695/149‑1 (MQ=255)
                cATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGtt                                                                                                                                   >  1:1211599/1‑151 (MQ=255)
                cATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGtt                                                                                                                                   >  1:84159/1‑151 (MQ=255)
                cATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGtt                                                                                                                                   >  1:739957/1‑151 (MQ=255)
                 aTAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTc                                                                                                                                  >  2:930337/1‑151 (MQ=255)
                  tAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGtt                                                                                                                                   <  4:365633/149‑1 (MQ=255)
                     ttATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACg                                                                                                                              >  2:1631764/1‑151 (MQ=255)
                        tCCCCGCATATCATTTATAAATTGACGTAAAACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGCTTCCGGTTCAACGCa                                                                                                                            >  1:303825/1‑150 (MQ=255)
                         ccccGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCaa                                                                                                                           <  2:956224/150‑1 (MQ=255)
                          cccGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAAc                                                                                                                         >  2:406188/1‑151 (MQ=255)
                          cccGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAAc                                                                                                                         >  2:706149/1‑151 (MQ=255)
                           ccGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTGCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAAcc                                                                                                                        <  1:104611/151‑1 (MQ=255)
                            cGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCg                                                                                                                       <  2:1138376/151‑1 (MQ=255)
                            cGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCg                                                                                                                       <  2:1028442/151‑1 (MQ=255)
                             gAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGc                                                                                                                      <  1:825363/151‑1 (MQ=255)
                               atatCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCaa                                                                                                                    <  2:348652/151‑1 (MQ=255)
                               atatCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCa                                                                                                                     >  2:833396/1‑150 (MQ=255)
                                   cATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGcc                                                                                                                <  3:9425/151‑1 (MQ=255)
                                   cATTTATAAATTGACGTAACACGTTATCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGcc                                                                                                                >  1:1349328/1‑151 (MQ=255)
                                    aTTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGc                                                                                                                 <  2:607274/149‑1 (MQ=255)
                                     tttATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCg                                                                                                              >  2:392207/1‑151 (MQ=255)
                                      ttATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCgg                                                                                                             >  3:108786/1‑151 (MQ=255)
                                        ataAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGa                                                                                                            >  1:1533444/1‑150 (MQ=255)
                                          aaaTTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGaaa                                                                                                          <  2:1522772/150‑1 (MQ=255)
                                          aaaTTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGaa                                                                                                           >  1:1402364/1‑149 (MQ=255)
                                          aaaTTGACGCAACCCGTTCTCTTTATGGAGCGTGCGTTCGGCATATTCCCGTGCCACCGTTTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGaa                                                                                                           <  1:1314338/149‑1 (MQ=255)
                                               gACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGttt                                                                                                     <  1:1177170/150‑1 (MQ=255)
                                                 cGTAACACGTTCTCTTTATCGAGCGTGCGTTCGTCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGACCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCg                                                                                                   <  1:1643594/150‑1 (MQ=255)
                                                  gTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCa                                                                                                 <  2:1059347/151‑1 (MQ=255)
                                                    aaCACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCaa                                                                                                >  4:279230/1‑150 (MQ=255)
                                                    aaCACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCaa                                                                                                >  2:1232776/1‑150 (MQ=255)
                                                     acacGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCaaa                                                                                               <  1:408269/150‑1 (MQ=255)
                                                      cacGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGc                                                                                             >  3:503960/1‑151 (MQ=255)
                                                        cGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTg                                                                                           >  1:1353822/1‑151 (MQ=255)
                                                        cGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTg                                                                                           >  2:301502/1‑151 (MQ=255)
                                                         gTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGc                                                                                          >  2:1358568/1‑151 (MQ=255)
                                                              ctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcccc                                                                                       <  2:1527535/149‑1 (MQ=255)
                                                              ctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGGTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGcccc                                                                                       >  2:494995/1‑149 (MQ=255)
                                                                ttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAg                                                                                     <  1:1153210/149‑1 (MQ=255)
                                                                 tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTc                                                                                  >  1:1469618/1‑151 (MQ=255)
                                                                 tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTc                                                                                  >  2:918197/1‑151 (MQ=255)
                                                                  aTCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCt                                                                                 >  3:343282/1‑151 (MQ=255)
                                                                   tCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtg                                                                                >  1:1525555/1‑151 (MQ=255)
                                                                    cGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgt                                                                               <  2:810975/151‑1 (MQ=255)
                                                                    cGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtg                                                                                <  2:1631431/150‑1 (MQ=255)
                                                                     gAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgtg                                                                              >  3:509733/1‑151 (MQ=255)
                                                                     gAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgtg                                                                              >  1:1364603/1‑151 (MQ=255)
                                                                     gAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCACCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGCTTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtgtg                                                                              >  1:1472474/1‑151 (MQ=255)
                                                                      aGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTgtgt                                                                             >  1:1124663/1‑151 (MQ=255)
                                                                       gcgtgcgtTCGTCATATTCACGTGCCACCGTGTTTTGTTTGGGCAGCAGGAGCGCCTTACGGATCCCCGCCTCCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTgtgt                                                                             <  4:236754/150‑1 (MQ=255)
                                                                       gcgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTa                                                                            <  1:740124/151‑1 (MQ=255)
                                                                           gcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCt                                                                        >  2:1702989/1‑151 (MQ=255)
                                                                            cgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCtt                                                                       <  3:224417/151‑1 (MQ=255)
                                                                             gtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGc                                                                         <  1:1421643/148‑1 (MQ=255)
                                                                              ttCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTc                                                                      <  4:129445/150‑1 (MQ=255)
                                                                                           cGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACcg                                                        <  1:1562234/151‑1 (MQ=255)
                                                                                               ccACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                                    <  1:194425/151‑1 (MQ=255)
                                                                                                cACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTa                                                   <  1:554690/151‑1 (MQ=255)
                                                                                                    gtgttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGc                                               >  1:642555/1‑151 (MQ=255)
                                                                                                     tgttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGcc                                              >  1:1492971/1‑151 (MQ=255)
                                                                                                       ttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCt                                             <  4:248744/150‑1 (MQ=255)
                                                                                                       ttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCt                                             <  4:185642/150‑1 (MQ=255)
                                                                                                          tgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTg                                         >  2:1307996/1‑151 (MQ=255)
                                                                                                           gttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGc                                        <  1:1571503/151‑1 (MQ=255)
                                                                                                            tttGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGc                                        <  4:310854/150‑1 (MQ=255)
                                                                                                             ttGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGcc                                       >  1:36931/1‑150 (MQ=255)
                                                                                                               gggCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAg                                     <  1:232801/150‑1 (MQ=255)
                                                                                                                ggCAGCAGGAGCGCCTGGCGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGa                                    <  2:1086795/150‑1 (MQ=255)
                                                                                                                ggCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGaa                                   <  1:849286/151‑1 (MQ=255)
                                                                                                                      agGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTgg                             <  2:591332/151‑1 (MQ=255)
                                                                                                                      agGAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTgg                             >  4:355821/1‑151 (MQ=255)
                                                                                                                       ggAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTg                              <  1:898379/149‑1 (MQ=255)
                                                                                                                        gAGCGCCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTc                           >  1:228156/1‑151 (MQ=255)
                                                                                                                          gcgcCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCa                          <  1:1056909/150‑1 (MQ=255)
                                                                                                                          gcgaCTGCCGGACCCCCGCCACCAGCACCTCGACCGATTACGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTCCCGCCTACTGCCAGAATATTGGTCAg                         <  1:233282/151‑1 (MQ=255)
                                                                                                                            gcCTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGtt                       <  1:59794/151‑1 (MQ=255)
                                                                                                                             ccTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGttt                      <  1:936863/151‑1 (MQ=255)
                                                                                                                             ccTGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGttt                      >  1:922802/1‑151 (MQ=255)
                                                                                                                               tGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCg                    >  3:12973/1‑151 (MQ=255)
                                                                                                                               tGACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCg                    >  2:1488914/1‑151 (MQ=255)
                                                                                                                                gACGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCg                    <  1:1231214/150‑1 (MQ=255)
                                                                                                                                 aCGGACCCCCGCCCCCAGCACCTCGACCTATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAATCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCTCCTACTTCCAGATTATTGGTCAGTTTCGaa                  <  1:1298712/151‑2 (MQ=255)
                                                                                                                                  cGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACg                 >  3:235336/1‑151 (MQ=255)
                                                                                                                                  cGGATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACg                 >  2:549857/1‑151 (MQ=255)
                                                                                                                                  aggATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGAGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACg                 >  4:59751/2‑151 (MQ=255)
                                                                                                                                   ggATCCCCGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACgg                <  2:1180512/151‑1 (MQ=255)
                                                                                                                                        cccGCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGAct                 <  1:716335/145‑2 (MQ=255)
                                                                                                                                           gCCACCAGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACgg        >  1:592258/1‑151 (MQ=255)
                                                                                                                                             caccaGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACgga       >  1:1294480/1‑149 (MQ=255)
                                                                                                                                               ccaGCACCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATc    >  4:57864/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGAGTTTCGACTGCGCCATAATTATCCCCGAATATCATTTATAAATTGACGTAACACGTTCTCTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTG  >  NC_000913/2124502‑2124797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: