Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,136,954 A→G V97A (GTC→GCC)  wza ← colanic acid export protein; outer membrane auxillary lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,136,9540AG99.2% 426.3 / ‑4.4 122V97A (GTC→GCC) wzacolanic acid export protein; outer membrane auxillary lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (71/50);  total (71/51)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 122 total aligned reads displayed.

ACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACATTCACCATTT  >  NC_000913/2136806‑2137101
                                                                                                                                                    |                                                                                                                                                   
aCTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCg                                                                                                                                                   <  4:255824/151‑1 (MQ=255)
aCTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCg                                                                                                                                                   <  4:426330/151‑1 (MQ=255)
     ggATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGAc                                                                                                                                              >  2:883422/1‑151 (MQ=255)
          tCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTa                                                                                                                                          <  2:1531692/150‑1 (MQ=255)
           cAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTa                                                                                                                                          >  2:1701161/1‑149 (MQ=255)
           cAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAg                                                                                                                                        <  1:656730/151‑1 (MQ=255)
           cAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCAGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTaa                                                                                                                                         <  2:1151994/150‑1 (MQ=255)
            agcgagcgTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGGGATCCCAGGCGGTGACATTa                                                                                                                                          >  1:1675066/1‑148 (MQ=255)
               gagcgTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACg                                                                                                                                    >  1:682715/1‑151 (MQ=255)
                  cgTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTcc                                                                                                                                 >  4:135327/1‑151 (MQ=255)
                  cgTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTAAAATTAAGAACGTc                                                                                                                                  >  2:1605535/1‑150 (MQ=255)
                      ttCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTccccc                                                                                                                              <  2:1139484/150‑1 (MQ=255)
                       tCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCg                                                                                                                             <  1:866902/150‑1 (MQ=255)
                          cGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGcgc                                                                                                                         <  2:39399/151‑3 (MQ=255)
                            aCTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCg                                                                                                                        >  2:594603/1‑150 (MQ=255)
                            aCTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCg                                                                                                                        >  2:1316883/1‑150 (MQ=255)
                             cTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGa                                                                                                                       <  4:351224/150‑1 (MQ=255)
                               aCGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGAcgc                                                                                                                    <  4:584631/151‑1 (MQ=255)
                                cGTGGACCTTGCCGATATACGGGTAAAACATAGAGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGAcgcg                                                                                                                   >  3:391161/1‑151 (MQ=255)
                                  tGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGActc                                                                                                                    <  1:1572825/148‑3 (MQ=255)
                                       cTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTgg                                                                                                            >  1:1337346/1‑151 (MQ=255)
                                         tGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTgg                                                                                                            <  2:249203/149‑1 (MQ=255)
                                         tGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGt                                                                                                           >  2:1352253/1‑150 (MQ=255)
                                           ccGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAAt                                                                                                        >  2:1187688/1‑151 (MQ=255)
                                           ccGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAAt                                                                                                        >  3:3661/1‑151 (MQ=255)
                                            cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTTAGTTCCGGGTGATCCCAGGCGGGGACATTAAGAACGTCCCCCGGCCCGACCCGATACTGGTAAtt                                                                                                       >  1:576896/1‑151 (MQ=255)
                                            cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAAtt                                                                                                       >  2:1551862/1‑151 (MQ=255)
                                            cGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGGAAtt                                                                                                       >  4:150984/1‑151 (MQ=255)
                                              atatACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGGGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTcg                                                                                                     >  2:1712618/1‑151 (MQ=255)
                                               tataCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGCGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTcgc                                                                                                    >  1:1374463/1‑151 (MQ=255)
                                                ataCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTcgc                                                                                                    <  2:1525570/150‑1 (MQ=255)
                                                 taCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTcgc                                                                                                    >  4:281527/1‑149 (MQ=255)
                                                  aCGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGAt                                                                                                 <  2:516964/151‑1 (MQ=255)
                                                      gTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCa                                                                                             <  2:660759/151‑1 (MQ=255)
                                                      gTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGCGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGAtctc                                                                                              >  2:196311/1‑150 (MQ=255)
                                                       tAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCAc                                                                                            >  2:110461/1‑151 (MQ=255)
                                                       tAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCAc                                                                                            >  1:137124/1‑151 (MQ=255)
                                                       tAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCAc                                                                                            >  2:745237/1‑151 (MQ=255)
                                                        aaaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACt                                                                                           >  2:656892/1‑151 (MQ=255)
                                                         aaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACtt                                                                                          >  1:211863/1‑151 (MQ=255)
                                                         aaaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACtt                                                                                          <  2:365317/151‑1 (MQ=255)
                                                          aaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACttt                                                                                         >  4:260348/1‑151 (MQ=255)
                                                          aaCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACttt                                                                                         >  2:1342008/1‑151 (MQ=255)
                                                           aCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACttt                                                                                         >  4:393865/1‑150 (MQ=255)
                                                           aCATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTc                                                                                        >  1:1710410/1‑151 (MQ=255)
                                                             aTAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTcc                                                                                       >  3:240562/1‑150 (MQ=255)
                                                               aGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGGGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATGCTGGTAATTCTCGATCTCACTTTCAAGc                                                                                    >  1:1228694/1‑151 (MQ=255)
                                                                  gCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGCGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCg                                                                                   >  2:195524/1‑149 (MQ=255)
                                                                   ccGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCa                                                                                <  1:1659006/151‑1 (MQ=255)
                                                                    cGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCat                                                                               >  2:1080054/1‑151 (MQ=255)
                                                                     gTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCata                                                                              <  1:842121/151‑1 (MQ=255)
                                                                       cAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATAtt                                                                            <  2:118475/151‑1 (MQ=255)
                                                                        aGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATAtt                                                                            >  1:906863/1‑150 (MQ=255)
                                                                        aGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTg                                                                           <  1:117582/151‑1 (MQ=255)
                                                                            tGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTgggg                                                                        >  1:920122/1‑150 (MQ=255)
                                                                            tGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGgc                                                                       >  3:38107/1‑151 (MQ=255)
                                                                               aCCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGgcgcgc                                                                   >  2:1593553/1‑151 (MQ=255)
                                                                                 ccAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCt                                                                  <  4:244840/151‑1 (MQ=255)
                                                                                 ccAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCt                                                                  <  2:9887/151‑1 (MQ=255)
                                                                                  cAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTa                                                                 <  2:736090/151‑1 (MQ=255)
                                                                                   aGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGGGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGAAACTGGTAATTCGCGATCTCACTTTCCAGCGGCATATTgggg                                                                        >  1:824360/1‑143 (MQ=255)
                                                                                    gTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACa                                                               <  1:1195947/151‑1 (MQ=255)
                                                                                      tGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGGAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACAtt                                                             >  2:279640/1‑151 (MQ=255)
                                                                                       gCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACAtt                                                             <  1:883307/150‑1 (MQ=255)
                                                                                        ccGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTc                                                            <  1:1002360/150‑1 (MQ=255)
                                                                                           gtgtCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggc                                                        <  1:546832/151‑1 (MQ=255)
                                                                                           gtgtCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggc                                                        <  2:709758/151‑1 (MQ=255)
                                                                                            tgtCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcg                                                       >  1:1539916/1‑151 (MQ=255)
                                                                                             gtCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgt                                                      >  1:796853/1‑151 (MQ=255)
                                                                                              tCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgt                                                      >  1:1698557/1‑150 (MQ=255)
                                                                                              tCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGATACATTCgggcgtg                                                     >  1:437693/1‑151 (MQ=255)
                                                                                               cGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgg                                                    <  2:1343071/151‑1 (MQ=255)
                                                                                                  tGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGCTCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggcg                                                 >  3:422837/1‑151 (MQ=255)
                                                                                                  tGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggcg                                                 >  1:1190394/1‑151 (MQ=255)
                                                                                                   ggAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggcg                                                 >  1:1155880/1‑150 (MQ=255)
                                                                                                   ggAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCgggcgtgggc                                                  <  3:329680/149‑1 (MQ=255)
                                                                                                    gAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTa                                               <  1:981598/151‑1 (MQ=255)
                                                                                                        tGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTg                                           >  2:1000994/1‑151 (MQ=255)
                                                                                                        tGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTg                                           >  2:541191/1‑151 (MQ=255)
                                                                                                         gCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAAtt                                            <  1:1296973/149‑1 (MQ=255)
                                                                                                            gTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTc                                        >  2:1487123/1‑150 (MQ=255)
                                                                                                                 gACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCa                                   <  2:682813/150‑1 (MQ=255)
                                                                                                                 gACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAg                                  <  2:1523872/151‑1 (MQ=255)
                                                                                                                      gCTGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGcgcg                             >  1:271764/1‑151 (MQ=255)
                                                                                                                        tGGCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCggg                           >  2:988278/1‑151 (MQ=255)
                                                                                                                          gCGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGt                         <  2:1680999/151‑1 (MQ=255)
                                                                                                                           cGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTc                        >  3:552410/1‑151 (MQ=255)
                                                                                                                           cGTGGTGAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTc                        <  1:745128/151‑1 (MQ=255)
                                                                                                                            gtggtgAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTc                        >  4:246334/1‑150 (MQ=255)
                                                                                                                               gtgAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCg                    <  1:1700995/151‑1 (MQ=255)
                                                                                                                               gtgAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCg                    <  2:776779/151‑1 (MQ=255)
                                                                                                                                tgAGTTCCGTGTGATCCCAGGCGGTGCGATTAAGAACGCCCCTGGAGCCGACGCGATACTGGTAATTGGCGATCTCTCTTCCCAGCGTCATATTGGGGCGCGCTGCATTCGGGCGTGGGGGTAATTGGGCAATGATGCGCggggcgag                      <  1:1557824/148‑5 (MQ=255)
                                                                                                                                 gAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCgg                   >  1:822038/1‑150 (MQ=255)
                                                                                                                                 gAGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGa                  <  1:539400/151‑1 (MQ=255)
                                                                                                                                  aGTTCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGAt                 <  2:814978/151‑1 (MQ=255)
                                                                                                                                     tCCGGGTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATaa               >  2:116810/1‑150 (MQ=255)
                                                                                                                                        gggTGATCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCTTCATATTGGGGCGCGCTACATTCGGGCGTCGGCGAAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACAt           <  1:1510476/151‑1 (MQ=255)
                                                                                                                                             aTCCCAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACATTCAcc      >  1:395976/1‑151 (MQ=255)
                                                                                                                                                ccAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACATTCACCAtt   >  2:653770/1‑151 (MQ=255)
                                                                                                                                                 cAGGCGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACATTCACCAttt  <  3:155479/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCCGGCCCGACGCGATACTGGTAATTCGCGATCTCACTTTCCAGCGTCATATTGGGGCGCGCTACATTCGGGCGTGGGCGTAATTGGTCAATCAGGCGCGGGGTCAGCGGATAAACATTCACCATTT  >  NC_000913/2136806‑2137101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: