Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,224,908 C→T intergenic (‑38/+136) pbpG ← / ← yohC D‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,224,9080CT99.3% 570.2 / ‑4.8 151intergenic (‑38/+136)pbpG/yohCD‑alanyl‑D‑alanine endopeptidase/Yip1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (72/78);  total (72/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 152 total aligned reads displayed.

TCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAAC  >  NC_000913/2224761‑2225054
                                                                                                                                                   |                                                                                                                                                  
tCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTgtgc                                                                                                                                                  >  1:228462/1‑150 (MQ=255)
 ccGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAc                                                                                                                                                >  2:526838/1‑151 (MQ=255)
 ccGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAc                                                                                                                                                >  2:888414/1‑151 (MQ=255)
     ttGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAccgcc                                                                                                                                            >  4:289578/1‑151 (MQ=255)
       gtgAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCAccgccg                                                                                                                                           <  1:831991/150‑1 (MQ=255)
         gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGa                                                                                                                                         <  2:1519846/150‑1 (MQ=255)
         gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAg                                                                                                                                        <  2:987777/151‑1 (MQ=255)
         gAAGCGGGGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGCGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTACATAAAGACACTCGAAATTTCGGCATGATGAGCTTTCAGATAGTGGGTCACGCGCAAGGGTTGTGCAccg                                                                                                                                              >  1:1264417/1‑145 (MQ=255)
            gCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTa                                                                                                                                      >  2:752413/1‑150 (MQ=255)
                tggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGAt                                                                                                                                 >  1:951535/1‑151 (MQ=255)
                 ggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGAtt                                                                                                                                <  2:1303240/151‑1 (MQ=255)
                   tAGCGGCTGCCGTCTTCTCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTCAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGAttt                                                                                                                               >  1:176066/1‑150 (MQ=255)
                    aGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTa                                                                                                                              <  2:1068005/150‑1 (MQ=255)
                    aGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTAc                                                                                                                             >  1:815696/1‑151 (MQ=255)
                     gCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTAc                                                                                                                             <  1:461489/150‑1 (MQ=255)
                         cTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAgg                                                                                                                        >  1:835325/1‑151 (MQ=255)
                                tttCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCg                                                                                                                  >  2:1262844/1‑150 (MQ=255)
                                        cGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatc                                                                                                          <  2:1354389/150‑1 (MQ=255)
                                         ggCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatcat                                                                                                        >  1:1248365/1‑151 (MQ=255)
                                          gCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                       <  3:29916/151‑1 (MQ=255)
                                          gCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                       <  1:308758/151‑1 (MQ=255)
                                          gCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                       <  1:1187086/151‑1 (MQ=255)
                                           ccTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                       >  1:825836/1‑150 (MQ=255)
                                           ccTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATa                                                                                                       >  4:253268/1‑150 (MQ=255)
                                           ccTGCGGTGAAAAAGGCACAGCCAGCATCAGGGCCAAGCTAATTAAAGAAACCCGGAATTACGGCATGATGAGCATTCAGATAGTGGTTAACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTAACGGAGTCTAGCGACGCcatca                                                                                                         >  1:387434/1‑148 (MQ=255)
                                              gCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                                                                                                    <  2:801358/150‑1 (MQ=255)
                                               cGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCTTGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGTATTTACTGCGTCTAGCGACGCCATCATAACg                                                                                                    >  1:1564411/1‑149 (MQ=255)
                                                ggTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGc                                                                                                 <  1:473975/151‑1 (MQ=255)
                                                    cAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaa                                                                                             <  3:468998/151‑1 (MQ=255)
                                                    cAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaa                                                                                             <  3:442501/151‑1 (MQ=255)
                                                     aaaaGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaa                                                                                             >  2:1468819/1‑150 (MQ=255)
                                                        aGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg                                                                                         >  1:997334/1‑151 (MQ=255)
                                                         ggCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGc                                                                                        <  1:299462/151‑1 (MQ=255)
                                                                  agcATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGACGGAGTAAGGAGTTACTGAGGCTAGCGACGCCATCAAAACGAGCAAAAAGTGCGATCGTc                                                                                 <  1:1327762/149‑1 (MQ=255)
                                                                     aTCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAg                                                                             <  1:214367/150‑1 (MQ=255)
                                                                      tCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAgg                                                                            >  1:701316/1‑150 (MQ=255)
                                                                       cAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAgg                                                                            >  2:1145056/1‑149 (MQ=255)
                                                                       cAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGgag                                                                          <  2:711192/151‑1 (MQ=255)
                                                                       cAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGga                                                                           <  1:964292/150‑1 (MQ=255)
                                                                         gggCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGgaga                                                                         >  4:249777/1‑150 (MQ=255)
                                                                         gggCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGaa                                                                        <  1:122414/151‑1 (MQ=255)
                                                                          ggCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAAt                                                                       <  3:390676/151‑1 (MQ=255)
                                                                            ccAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACTGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCg                                                                     <  1:1035749/151‑1 (MQ=255)
                                                                            ccAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCg                                                                     <  1:542282/151‑1 (MQ=255)
                                                                             cAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGt                                                                    >  3:45023/1‑151 (MQ=255)
                                                                                 cTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                >  2:762234/1‑151 (MQ=255)
                                                                                 cTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                >  3:6357/1‑151 (MQ=255)
                                                                                  taaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAgg                                                                >  1:92329/1‑150 (MQ=255)
                                                                                   aaataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                              <  2:1235367/151‑1 (MQ=255)
                                                                                    aataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGaa                                                              >  2:1590004/1‑150 (MQ=255)
                                                                                     ataaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACCGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAt                                                            <  2:172441/151‑1 (MQ=255)
                                                                                      taaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtg                                                           >  1:305325/1‑151 (MQ=255)
                                                                                       aaaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgc                                                          <  1:175431/151‑1 (MQ=255)
                                                                                        aaGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgct                                                         >  3:446504/1‑151 (MQ=255)
                                                                                         aGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctg                                                        >  1:1467592/1‑151 (MQ=255)
                                                                                         aGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAAtgctg                                                        >  1:11570/1‑151 (MQ=255)
                                                                                             aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                                    <  1:1293812/151‑1 (MQ=255)
                                                                                             aCTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAtt                                                    >  4:156158/1‑151 (MQ=255)
                                                                                                cGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTg                                                   <  1:935348/149‑1 (MQ=255)
                                                                                                cGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCt                                                 <  2:915851/151‑1 (MQ=255)
                                                                                                cGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCt                                                 >  1:569265/1‑151 (MQ=255)
                                                                                                 gAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                <  4:33136/151‑1 (MQ=255)
                                                                                                 gAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTg                                                <  2:585841/151‑1 (MQ=255)
                                                                                                   aaTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGAc                                              >  1:1053193/1‑151 (MQ=255)
                                                                                                    aTTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACa                                             <  3:258038/151‑1 (MQ=255)
                                                                                                      ttCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAtt                                           >  3:370277/1‑151 (MQ=255)
                                                                                                      ttCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAtt                                           >  1:693063/1‑151 (MQ=255)
                                                                                                      ttCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAt                                            <  4:309363/150‑1 (MQ=255)
                                                                                                      ttCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAt                                            <  2:6644/150‑1 (MQ=255)
                                                                                                        cGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAttt                                          >  2:537887/1‑150 (MQ=255)
                                                                                                        cGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACAttt                                          >  2:1669924/1‑150 (MQ=255)
                                                                                                            atgatgAGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGcc                                     >  3:199081/1‑151 (MQ=255)
                                                                                                              gatgaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAg                                   <  1:1233741/151‑1 (MQ=255)
                                                                                                                 gaGCATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCaa                                <  1:763434/151‑1 (MQ=255)
                                                                                                                    cATTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGc                             >  1:1183451/1‑151 (MQ=255)
                                                                                                                     aTTCAGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCa                            <  2:832308/151‑1 (MQ=255)
                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                        <  2:228499/151‑1 (MQ=255)
                                                                                                                         aGATAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGt                        >  3:266619/1‑151 (MQ=255)
                                                                                                                           aTAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCa                      <  2:201906/151‑1 (MQ=255)
                                                                                                                            tAGTGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCa                      >  2:1175014/1‑150 (MQ=255)
                                                                                                                               tGGTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCaa                     >  4:56406/1‑148 (MQ=255)
                                                                                                                                ggTTCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGa                  >  2:13643/1‑150 (MQ=255)
                                                                                                                                   tCACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCTTAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACt               <  4:379831/150‑1 (MQ=255)
                                                                                                                                    cACGCGCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACtt              <  2:1605995/150‑1 (MQ=255)
                                                                                                                                      cgcgCACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt           >  2:817594/1‑151 (MQ=255)
                                                                                                                                       gcgcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt           >  3:340630/1‑150 (MQ=255)
                                                                                                                                       gcgcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTa          <  1:607397/151‑1 (MQ=255)
                                                                                                                                       gcgcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTa          <  1:1174875/151‑1 (MQ=255)
                                                                                                                                       gcgcACGGGTTGTGCACCGCCGGAATAAGGATTTACTGAGGCTAGAGACGCCATCATAACGAGCAAAAAGTACGATCGTCAAAGGAGAAACGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGAAACGTCAAAGAACATTc            <  1:787803/149‑1 (MQ=255)
                                                                                                                                         gcACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCt           >  1:1707290/1‑148 (MQ=255)
                                                                                                                                          cACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGa        <  2:1175870/150‑1 (MQ=255)
                                                                                                                                          cACGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGa        >  1:35627/1‑150 (MQ=255)
                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGaa       <  2:287981/150‑1 (MQ=255)
                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc      <  1:526755/151‑1 (MQ=255)
                                                                                                                                           aCGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAAc      <  3:525859/151‑1 (MQ=255)
                                                                                                                                            cGGGTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACa     <  2:951655/151‑1 (MQ=255)
                                                                                                                                             gggTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACaa    <  2:308321/151‑1 (MQ=255)
                                                                                                                                             gggTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACaa    <  1:941768/151‑1 (MQ=255)
                                                                                                                                               gTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAAc  >  1:170297/1‑151 (MQ=255)
                                                                                                                                               gTTGTGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAAc  <  3:138972/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAAC  >  NC_000913/2224761‑2225054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: