Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,241,409 T→C Q87R (CAG→CGG)  galS ← galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,241,4090TC99.3% 531.1 / ‑4.0 141Q87R (CAG→CGG) galSgalactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (80/60);  total (81/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 145 total aligned reads displayed.

CCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGG  >  NC_000913/2241263‑2241553
                                                                                                                                                  |                                                                                                                                                
ccATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATgccgct                                                                                                                                               <  1:975963/150‑1 (MQ=255)
 cATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATgccgct                                                                                                                                               >  1:598977/1‑149 (MQ=255)
 cATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGccgctg                                                                                                                                              >  1:863863/1‑150 (MQ=255)
 cATAAATTGCGCCAGTTCAACGTCACGCAATTCATTTGAGTGAACAATCATCGCATTACAACGCTGGCGCATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGAATGGCACGTATCTCTGATGCCGCAGa                                                                                                                                             <  1:849508/151‑1 (MQ=255)
   tAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTACCACGTATTTCTGATGCCGCTGa                                                                                                                                             >  1:525824/1‑149 (MQ=255)
    aaaTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCg                                                                                                                                          <  4:17434/151‑1 (MQ=255)
     aaTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCg                                                                                                                                          >  1:208395/1‑150 (MQ=255)
     aaTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCg                                                                                                                                          >  1:674853/1‑150 (MQ=255)
      aTTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGAc                                                                                                                                        <  1:1042702/151‑1 (MQ=255)
        tGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGAcc                                                                                                                                       >  2:853241/1‑150 (MQ=255)
        aacgcgAGTGCATCGTCACTGAGGGGATTGGAGTGAACAATGAACGCATGACAAGGCTGGCGGGTTAACACCTCAATAAAGAGAAGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGATGAGCGAc                                                                                                                                        >  4:13230/3‑149 (MQ=255)
         gcgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGAcc                                                                                                                                       >  2:126592/1‑149 (MQ=255)
         gcgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCa                                                                                                                                      <  1:395071/150‑1 (MQ=255)
         gcgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCa                                                                                                                                      <  2:1267097/150‑1 (MQ=255)
          cgcCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCGGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGa                                                                                                                                    <  1:679524/151‑1 (MQ=255)
             cAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATcc                                                                                                                                 <  2:386904/151‑1 (MQ=255)
             cAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATc                                                                                                                                  >  3:368703/1‑150 (MQ=255)
               gTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCAc                                                                                                                               >  4:230063/1‑151 (MQ=255)
                  cATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGc                                                                                                                            >  1:1718274/1‑151 (MQ=255)
                   aTCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCt                                                                                                                           >  1:1544598/1‑151 (MQ=255)
                   aTCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCt                                                                                                                           >  4:134451/1‑151 (MQ=255)
                     cGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCttt                                                                                                                         >  1:1016504/1‑151 (MQ=255)
                            cAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACTCCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCGGAGCGACCAGATCCACCGCTTTTACCAg                                                                                                                   <  1:1137307/150‑1 (MQ=255)
                              aTGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAgcgc                                                                                                                <  1:625151/151‑1 (MQ=255)
                              aTGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAgcgc                                                                                                                >  1:464095/1‑151 (MQ=255)
                                  ttttGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCg                                                                                                            >  2:1064751/1‑151 (MQ=255)
                                      gAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaa                                                                                                         <  2:1243915/150‑1 (MQ=255)
                                       aGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaaa                                                                                                        >  2:1204082/1‑150 (MQ=255)
                                       aGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaaa                                                                                                        >  2:228901/1‑150 (MQ=255)
                                       aGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGaaaa                                                                                                        >  3:259871/1‑150 (MQ=255)
                                          gAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACg                                                                                                     <  1:1221339/150‑1 (MQ=255)
                                              aaTCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCa                                                                                                <  1:552871/151‑1 (MQ=255)
                                               aTCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAg                                                                                               <  1:1437222/151‑1 (MQ=255)
                                                tCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATc                                                                                                 >  4:245059/1‑148 (MQ=255)
                                                  aaCGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGaaa                                                                                            <  2:1337301/151‑1 (MQ=255)
                                                         tACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCa                                                                                      <  3:222409/150‑1 (MQ=255)
                                                         tACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCAt                                                                                     >  1:1176337/1‑151 (MQ=255)
                                                         tACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCAt                                                                                     >  4:580487/1‑151 (MQ=255)
                                                          aCAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCAt                                                                                     <  1:1430297/150‑1 (MQ=255)
                                                            aaCGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcac                                                                                  <  2:1688753/151‑1 (MQ=255)
                                                              cGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcacca                                                                                <  3:435568/151‑1 (MQ=255)
                                                              cGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcacca                                                                                <  1:999273/151‑1 (MQ=255)
                                                              cGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcacc                                                                                 >  4:318481/1‑150 (MQ=255)
                                                                 tGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATcaccacc                                                                              >  2:1234881/1‑150 (MQ=255)
                                                                   gCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACg                                                                           <  1:506030/151‑1 (MQ=255)
                                                                   gCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACg                                                                           >  2:1370929/1‑151 (MQ=255)
                                                                     gAATTAACACCTCACTGGCGTGGCGCCCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACTAGATCCACCGCTTTTACCAACGCGCCAAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                         >  4:557359/1‑151 (MQ=255)
                                                                     gAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                         >  1:937374/1‑151 (MQ=255)
                                                                      aaTTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                         >  1:1175357/1‑150 (MQ=255)
                                                                      aaTTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGcc                                                                         >  1:879534/1‑150 (MQ=255)
                                                                      aaTTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCa                                                                        <  2:178652/151‑1 (MQ=255)
                                                                        ttAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAAt                                                                      >  2:974164/1‑151 (MQ=255)
                                                                         tAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                     >  2:1154945/1‑151 (MQ=255)
                                                                         tAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                     >  1:1070747/1‑151 (MQ=255)
                                                                          aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCCCCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATgg                                                                    <  1:1397351/151‑1 (MQ=255)
                                                                          aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATgg                                                                    >  1:1552739/1‑151 (MQ=255)
                                                                          aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                     <  2:1551583/150‑1 (MQ=255)
                                                                          aaCACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATg                                                                     <  1:1398573/150‑1 (MQ=255)
                                                                           acacCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgt                                                                   >  3:259483/1‑151 (MQ=255)
                                                                           acacCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGgt                                                                   >  3:413985/1‑151 (MQ=255)
                                                                                 cAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCg                                                               <  3:548197/149‑1 (MQ=255)
                                                                                 cAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt                                                             <  2:256263/151‑1 (MQ=255)
                                                                                      gCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCt                                                             <  3:2673/146‑1 (MQ=255)
                                                                                       cGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTg                                                       <  2:1060515/151‑1 (MQ=255)
                                                                                          gACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGt                                                    <  2:804011/151‑1 (MQ=255)
                                                                                           aCGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGtt                                                   <  2:1586278/151‑1 (MQ=255)
                                                                                            cGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTg                                                  <  3:266425/151‑1 (MQ=255)
                                                                                               tctTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGcc                                                >  2:1108410/1‑150 (MQ=255)
                                                                                                  ttttCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAgcg                                            >  4:142536/1‑151 (MQ=255)
                                                                                                    ttCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAgcgc                                           <  3:232625/150‑1 (MQ=255)
                                                                                                    ttCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGcc                                          >  2:1412072/1‑151 (MQ=255)
                                                                                                     tCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCt                                         >  3:510042/1‑151 (MQ=255)
                                                                                                      ccGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTg                                        >  2:334351/1‑151 (MQ=255)
                                                                                                      ccGCTGCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCt                                         <  1:1265840/150‑1 (MQ=255)
                                                                                                        gCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg                                      >  2:1172974/1‑151 (MQ=255)
                                                                                                        gCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTggg                                      >  3:276538/1‑151 (MQ=255)
                                                                                                         cTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGCGTCGCTAACCTGAGTAGCCAGCGCCTgg                                       >  1:133123/1‑149 (MQ=255)
                                                                                                           tCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCa                                    <  4:289870/150‑1 (MQ=255)
                                                                                                            cATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCAtt                                  <  1:1700623/151‑1 (MQ=255)
                                                                                                            cATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCAt                                   <  1:131490/150‑1 (MQ=255)
                                                                                                             aTGATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTg                                 >  1:977553/1‑151 (MQ=255)
                                                                                                               gATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGc                               >  2:1503384/1‑151 (MQ=255)
                                                                                                               gATAGCTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGc                               >  1:746625/1‑151 (MQ=255)
                                                                                                                    cTATTGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTg                          >  2:600044/1‑151 (MQ=255)
                                                                                                                        tGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGcc                       <  2:969962/150‑1 (MQ=255)
                                                                                                                        tGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGca                       <  1:1692456/150‑2 (MQ=255)
                                                                                                                        tGCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCg                      >  1:1278380/1‑151 (MQ=255)
                                                                                                                         gCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCg                      >  1:1160565/1‑150 (MQ=255)
                                                                                                                         gCCGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCg                      >  1:433227/1‑150 (MQ=255)
                                                                                                                          ccGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAt                    >  1:820381/1‑151 (MQ=255)
                                                                                                                           cGATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGAt                    >  2:605387/1‑150 (MQ=255)
                                                                                                                            gATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATaa                  <  2:1314626/151‑1 (MQ=255)
                                                                                                                            gATTAGCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATaa                  <  1:1021004/151‑1 (MQ=255)
                                                                                                                                 gCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATcc               >  3:370610/1‑149 (MQ=255)
                                                                                                                                 gCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg             <  1:126568/151‑1 (MQ=255)
                                                                                                                                 gCACGTATTTCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAg             <  2:1130830/151‑1 (MQ=255)
                                                                                                                                         ttCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGctcactc      >  2:585623/1‑150 (MQ=255)
                                                                                                                                          tCTGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCtcactcac    >  2:1589644/1‑151 (MQ=255)
                                                                                                                                            tGATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCa                                    >  4:219695/1‑117 (MQ=255)
                                                                                                                                             gATGCCGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACgg  <  2:1718441/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCATAAATTGCGCCAGTTCATCGTCACTCAATGCTTTTGAGTGAACAATCAACGCATTACAACGCTGGCGAATTAACACCTCAATGGCGTGACGCTCTTTTTCCGCTTCATGATAGCTATTGCCGATTAGCACGTATTTCTGATGCTGCTGAGCGACCAGATCCACCGCTTTTACCAGCGCGCCGAAAAACGCATCAGAAACGTCCATCACCACCACGCCAATGGTGTCGCTAACCTGAGTTGCCAGCGCCTGGGCATTGGCGTTTGGCCGATAATCCAGCTCACTCACGG  >  NC_000913/2241263‑2241553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: