Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,258,247 A→G F41L (TTC→CTC)  psuG ← pseudouridine 5'‑phosphate glycosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,258,2470AG99.3% 467.3 / NA 137F41L (TTC→CTC) psuGpseudouridine 5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (62/73);  minor base T (0/1);  total (63/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 137 total aligned reads displayed.

TTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAACG  >  NC_000913/2258099‑2258394
                                                                                                                                                    |                                                                                                                                                   
 tATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCgg                                                                                                                                                  >  1:605229/1‑151 (MQ=255)
    gCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGc                                                                                                                                                 >  2:381698/1‑149 (MQ=255)
        ttCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATccc                                                                                                                                            >  2:714277/1‑150 (MQ=255)
           aCGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTga                                                                                                                                        <  2:1501541/151‑1 (MQ=255)
           aCGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTga                                                                                                                                        <  1:923240/151‑1 (MQ=255)
            cGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTga                                                                                                                                        <  1:548439/150‑1 (MQ=255)
              aCCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTTTTCAACTTCAATTGCGGTCTGGGCATTTTTTGGGAGCGGCATCCCGTgaga                                                                                                                                      >  1:1015806/1‑150 (MQ=255)
              aCCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGaa                                                                                                                                     <  2:711087/151‑1 (MQ=255)
                   gTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAaataat                                                                                                                                <  1:772669/151‑1 (MQ=255)
                        tCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTc                                                                                                                            <  2:30948/150‑1 (MQ=255)
                         cAATTTCTTCTTTGCTGAACCCCGCTTGCATCCCGCCGCCAATAATGGCGATCGTGTCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACGTCAATTGCGGTCTGGGCATTTTGTGGGATCGGCATCCCGTGAGAAATAATGGTCgt                                                                                                                          <  1:1578597/151‑2 (MQ=255)
                             ttcttcTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAGTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGGGAGAAATAATGGTCGAtt                                                                                                                        >  1:701126/1‑149 (MQ=255)
                              tcttctTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTcc                                                                                                                      >  2:1083492/1‑150 (MQ=255)
                              tcttctTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTcc                                                                                                                      >  2:567850/1‑150 (MQ=255)
                                 tcttTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAgcg                                                                                                                  >  2:289842/1‑151 (MQ=255)
                                 tcttTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTACGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCa                                                                                                                     <  2:1396100/148‑1 (MQ=255)
                                   tttGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGcc                                                                                                                >  2:467564/1‑151 (MQ=255)
                                   tttGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAACGCCCTGTTTTAGAAAAGGTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGGGCGGCATCCCGTGAGAAATAATTGTCGGTTCCAGCGcc                                                                                                                >  1:1315808/1‑151 (MQ=255)
                                     tGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCcac                                                                                                              >  3:281058/1‑151 (MQ=255)
                                      gCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCcaca                                                                                                             <  3:592590/151‑1 (MQ=255)
                                        ttAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGGAAAGCGCCCTGTTTATGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCCGCGCCACAAc                                                                                                           >  2:1287121/1‑151 (MQ=255)
                                         tAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACaa                                                                                                            <  3:192226/149‑1 (MQ=255)
                                           aCCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGGCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCCACCgg                                                                                                        >  4:584227/1‑151 (MQ=255)
                                             cccACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTTTTCAAATTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGgtt                                                                                                      >  3:510351/1‑151 (MQ=255)
                                              ccACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttt                                                                                                     <  2:1684240/151‑1 (MQ=255)
                                              ccACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttt                                                                                                     <  1:984309/151‑1 (MQ=255)
                                                aCTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttt                                                                                                   >  2:332420/1‑151 (MQ=255)
                                                   ttCCTCCCGGCGGCAATTATGGCCATTGTTGGAAGTTCCGCGGCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttg                                                                                                 <  2:324661/150‑1 (MQ=255)
                                                   ttCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTCTTTACGGATAGATTCTTCGACTTCAATTGCGGTCTGGGCATTTTTTGGGAGCGGCATCCCCTGAAGAATAATGGTCGGTTCCAGCGCCACGACCGgtttttttt                                                                                                >  2:156651/1‑149 (MQ=255)
                                                      atcaCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgtttt                                                                                             <  2:1543272/151‑1 (MQ=255)
                                                      atcaCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttt                                                                                              <  1:1302702/150‑1 (MQ=255)
                                                        caCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttttt                                                                                            <  1:251423/150‑1 (MQ=255)
                                                        caCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttttt                                                                                            <  1:1250259/150‑1 (MQ=255)
                                                        caCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttttt                                                                                            <  4:300468/150‑1 (MQ=255)
                                                        caCGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGgttttttgttttt                                                                                            <  2:226532/150‑1 (MQ=255)
                                                        caCGCCGCCAATAATGGCGATCGTGGCAGGCAAAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCCCAACCGgttttttgtttttt                                                                                           >  1:398455/1‑151 (MQ=255)
                                                          cgccgcCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTaa                                                                                         <  2:988505/151‑1 (MQ=255)
                                                                  ataatGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCt                                                                                 <  1:544387/151‑1 (MQ=255)
                                                                     atGGCGATCGTGGCAGGCCCAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGGGGTCTGGGGCTTTTGTGGGAGCGGCATCCCGGGAGAAATAATGGTCGATTGCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGt                                                                                    >  1:1391736/1‑145 (MQ=255)
                                                                      tGGCGATCGTGGCAGGGACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGGGCCACAACCGGCTTTTTGTTTTTTAAAGCGTCCTGCAc                                                                             >  2:389784/1‑151 (MQ=255)
                                                                       ggCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCAc                                                                             <  2:106282/150‑1 (MQ=255)
                                                                        gCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCAc                                                                             <  1:1610197/149‑1 (MQ=255)
                                                                         cGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTc                                                                          <  3:6321/151‑1 (MQ=255)
                                                                          gATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTcc                                                                         <  2:1297011/151‑1 (MQ=255)
                                                                                ggCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGa                                                                    <  1:664302/150‑1 (MQ=255)
                                                                                ggCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGa                                                                    <  4:281040/150‑1 (MQ=255)
                                                                                   aGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAAtt                                                                <  2:605313/151‑1 (MQ=255)
                                                                                    ggTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAAttt                                                               <  3:135218/151‑1 (MQ=255)
                                                                                         aGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGtaat                                                          <  2:1705893/151‑1 (MQ=255)
                                                                                         aGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGtaat                                                          <  1:935923/151‑1 (MQ=255)
                                                                                         aGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGtaat                                                          <  2:134001/151‑1 (MQ=255)
                                                                                           cgcCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCt                                                                                 <  3:350543/126‑1 (MQ=255)
                                                                                           cgcCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGtaataa                                                        <  1:1033304/151‑1 (MQ=255)
                                                                                            gcCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTaataat                                                       >  2:631642/1‑151 (MQ=255)
                                                                                             cccTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTaataat                                                       <  3:117043/150‑1 (MQ=255)
                                                                                             cccTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAAtt                                                      >  1:1343229/1‑151 (MQ=255)
                                                                                             cccTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAAtt                                                      <  1:531592/151‑1 (MQ=255)
                                                                                              ccTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTc                                                     >  1:461860/1‑151 (MQ=255)
                                                                                               cTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTc                                                     >  1:60325/1‑150 (MQ=255)
                                                                                                 gTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCa                                                    <  4:538599/149‑1 (MQ=255)
                                                                                                 gTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAgg                                                  >  1:64568/1‑151 (MQ=255)
                                                                                                  tttACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAggg                                                 >  2:972215/1‑151 (MQ=255)
                                                                                                  tttACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAgg                                                  <  1:105514/150‑1 (MQ=255)
                                                                                                   ttACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAggg                                                 <  1:379111/150‑1 (MQ=255)
                                                                                                    tACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAgggg                                                >  2:1591779/1‑150 (MQ=255)
                                                                                                     aCGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGa                                               <  2:1250845/150‑1 (MQ=255)
                                                                                                      cGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGaaa                                             <  2:1327717/151‑1 (MQ=255)
                                                                                                         aTAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAttt                                          <  2:770777/151‑1 (MQ=255)
                                                                                                          tAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtttt                                         >  2:614281/1‑151 (MQ=255)
                                                                                                           aGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAttttt                                        <  1:1726603/151‑1 (MQ=255)
                                                                                                           aGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAAtttt                                         <  2:198303/150‑1 (MQ=255)
                                                                                                             tttCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTa                                       >  4:512780/1‑150 (MQ=255)
                                                                                                                 ttcaacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTc                                   >  1:682682/1‑150 (MQ=255)
                                                                                                                   caacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAg                                 <  1:151120/150‑1 (MQ=255)
                                                                                                                   caacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAg                                 <  4:287870/150‑1 (MQ=255)
                                                                                                                   caacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGa                                <  2:1626518/151‑1 (MQ=255)
                                                                                                                    aacttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGAc                               <  2:173264/151‑1 (MQ=255)
                                                                                                                     acttcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACa                              <  2:1070760/151‑1 (MQ=255)
                                                                                                                     aattcacTGGCGGTCTGGGCCGTTGTTGGGAGCGGCATCACGTTAGAAATAATGGTCGATTCCAGAGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTc                                   <  1:1164486/144‑1 (MQ=255)
                                                                                                                        tcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACAtt                            <  2:904246/150‑1 (MQ=255)
                                                                                                                        tcaaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACAt                             <  1:1680060/149‑1 (MQ=255)
                                                                                                                         caaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTc                           >  1:487562/1‑150 (MQ=255)
                                                                                                                         caaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCt                          <  1:1532141/151‑1 (MQ=255)
                                                                                                                         caaTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCt                          >  1:44934/1‑151 (MQ=255)
                                                                                                                           aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGc                        <  2:1681803/151‑1 (MQ=255)
                                                                                                                           aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGc                        >  3:23939/1‑151 (MQ=255)
                                                                                                                           aTTGCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGc                        <  2:281258/151‑1 (MQ=255)
                                                                                                                              gCGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGtt                     <  1:628766/151‑1 (MQ=255)
                                                                                                                               cGGTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGtt                     <  2:191913/150‑1 (MQ=255)
                                                                                                                                ggTCTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTtct                   >  1:852066/1‑151 (MQ=255)
                                                                                                                                   cTGGGCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTcc                 >  1:840027/1‑150 (MQ=255)
                                                                                                                                   cTGGGCATTTTGTGGGACCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACATCCGGTTTTTTGCTTTTTAAAGTGTCCTGCACTTCCGGGGAACTTTGTCATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTtctc                  >  1:778206/1‑149 (MQ=255)
                                                                                                                                     gggCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTcc                 <  4:7603/148‑1 (MQ=255)
                                                                                                                                     gggCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCa                <  1:72681/149‑1 (MQ=255)
                                                                                                                                       gCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTaa            <  4:466475/151‑1 (MQ=255)
                                                                                                                                       gCATTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTa             >  1:799477/1‑150 (MQ=255)
                                                                                                                                         aTTTTGTGGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACg          <  1:1582669/151‑1 (MQ=255)
                                                                                                                                             tgtgGGAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGatat      >  3:523178/1‑151 (MQ=255)
                                                                                                                                                gggAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAAc   >  2:1192973/1‑151 (MQ=255)
                                                                                                                                                 ggAGCGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAACg  <  2:1186375/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTATGCCCTTCACGACCCAGTAATTCAATTTCTTCTTTGCTTAACCCCACTTTCATCACGCCGCCAATAATGGCGATCGTGGCAGGTACAGCGCCCTGTTTACGAATAGTTTCTTCAACTTCAATTGCGGTCTGGGCATTTTGTGGGAACGGCATCCCGTGAGAAATAATGGTCGATTCCAGCGCCACAACCGGTTTTTTGTTTTTTAAAGCGTCCTGCACTTCCGGGGAAATTTGTAATAATTCAGGGGAAATTTTTAATTCAGACATTCTGCGTTCTCCACTAACGATATAACG  >  NC_000913/2258099‑2258394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: