Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,498,862 T→C V64V (GTT→GTC)  ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,498,8620TC100.0% 491.5 / NA 137V64V (GTT→GTC) ypdAsensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (66/71);  total (66/71)

Only 100 of 137 total aligned reads displayed.

GCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCC  >  NC_000913/2498716‑2499009
                                                                                                                                                  |                                                                                                                                                   
 cGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGt                                                                                                                                                >  2:598453/1‑151 (MQ=255)
 cGTTAATGCTTATCTCCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCAAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGt                                                                                                                                                <  2:520173/151‑1 (MQ=255)
  gTTAATGCTTATCTGCCTGTTCTTTCTCACCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTa                                                                                                                                               <  1:599729/151‑1 (MQ=255)
     aaTGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGa                                                                                                                                             >  3:332913/1‑150 (MQ=255)
      aTGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGa                                                                                                                                             >  2:1130563/1‑149 (MQ=255)
      aTGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGa                                                                                                                                             >  3:557592/1‑149 (MQ=255)
      aTGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGa                                                                                                                                             >  2:127095/1‑149 (MQ=255)
         cTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCt                                                                                                                                        >  2:136286/1‑151 (MQ=255)
            aTCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCCCTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTc                                                                                                                                       <  1:1105359/149‑1 (MQ=255)
             tCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCg                                                                                                                                      <  2:357417/149‑1 (MQ=255)
                gCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTg                                                                                                                                   <  2:859702/149‑1 (MQ=255)
                 ccTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACATGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTg                                                                                                                                >  2:1059435/1‑151 (MQ=255)
                  cTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGCTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTg                                                                                                                                >  3:279894/1‑150 (MQ=255)
                     ttctttctCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAacg                                                                                                                            >  2:1443973/1‑151 (MQ=255)
                         ttctCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAacgt                                                                                                                           <  2:1145695/148‑1 (MQ=255)
                         ttctCATCCGTATCCGCCTGTTTCGCGAACTGCTGCACAAGTCGGCCCATTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAacgtacg                                                                                                                        >  1:156548/1‑151 (MQ=255)
                           ctcATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAacgtacg                                                                                                                        <  3:555514/149‑1 (MQ=255)
                             cATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGtatt                                                                                                                    <  2:842495/151‑1 (MQ=255)
                              aTCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGtatt                                                                                                                    >  4:367415/1‑150 (MQ=255)
                               tCCGTACCCGCCTTTTTCGCGACCTGTTGCTCAATTCGGCGCACTCCCCAAAATAATGGCTCGCCCTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCTCCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAAcgtacgta                                                                                                                      <  1:1038176/147‑1 (MQ=255)
                                 cGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATcg                                                                                                                <  2:755471/151‑1 (MQ=255)
                                  gTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATcgc                                                                                                               >  2:475958/1‑151 (MQ=255)
                                  gTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATcgc                                                                                                               <  2:992712/151‑1 (MQ=255)
                                  gTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATcgc                                                                                                               >  3:517192/1‑151 (MQ=255)
                                  gTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATc                                                                                                                 >  2:93683/1‑149 (MQ=255)
                                    aTCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCgg                                                                                                             >  1:360077/1‑151 (MQ=255)
                                      ccGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCgg                                                                                                             <  1:65039/149‑1 (MQ=255)
                                       cGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGa                                                                                                          <  1:1009268/151‑1 (MQ=255)
                                       cGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGa                                                                                                          <  2:1098844/151‑1 (MQ=255)
                                        gCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGAt                                                                                                         >  1:815359/1‑151 (MQ=255)
                                          cTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGt                                                                                                       >  4:573097/1‑151 (MQ=255)
                                          cTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGt                                                                                                       >  1:1200295/1‑151 (MQ=255)
                                              ttCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCcg                                                                                                    <  1:1476540/150‑1 (MQ=255)
                                                   gAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCggg                                                                                               >  2:678873/1‑150 (MQ=255)
                                                     aCTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGAt                                                                                             >  1:20181/1‑150 (MQ=255)
                                                           gCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGtt                                                                                       <  3:434596/150‑1 (MQ=255)
                                                            cacaAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTg                                                                                     <  2:473663/151‑1 (MQ=255)
                                                             acaAGTCGGCGCACTCCCCAAAAGAATAGCTCTCCTTTACCGCCATTTTCTCGCTGTTCTCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCGGTTGAACGTACGTATTATCGCTGTGATGTCCGGCGGGATTCTGTTTgg                                                                                    <  1:1337841/151‑1 (MQ=255)
                                                              caAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTTGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTgg                                                                                    >  2:630070/1‑150 (MQ=255)
                                                                      cgcACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGt                                                                           <  3:573117/151‑1 (MQ=255)
                                                                        cACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTa                                                                          <  2:1554873/150‑1 (MQ=255)
                                                                        cACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAg                                                                         <  1:448606/151‑1 (MQ=255)
                                                                            ccccAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGc                                                                       <  1:1455504/149‑1 (MQ=255)
                                                                            ccccAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGc                                                                       <  1:1284232/149‑1 (MQ=255)
                                                                                 aaaGAATTGCTCGCCTTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTa                                                                <  2:1676587/151‑1 (MQ=255)
                                                                                 aaaGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCAtt                                                                 >  4:555156/1‑150 (MQ=255)
                                                                                 aaaGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTa                                                                <  2:264644/151‑1 (MQ=255)
                                                                                     aaTTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCgg                                                            >  2:530721/1‑151 (MQ=255)
                                                                                           tCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGAt                                                      >  2:1269385/1‑151 (MQ=255)
                                                                                           tCGCCGCTACCGCCAATTCCTCGTTGTTCGACCAGTTCAGCAACTGGTCCGACGTCCCCGTAGCAGGCTCGCTGGTGAACGTCCGAATTATCGCGGTGATGTCCGGCGGGATTCTGTTTCGCCCGGGGGTAGGCGTCATTCTCGGCGTg                                                        >  1:730001/1‑149 (MQ=255)
                                                                                             gCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTg                                                    >  2:915489/1‑151 (MQ=255)
                                                                                               cGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCGCCGGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCg                                                  <  2:27915/151‑1 (MQ=255)
                                                                                               cGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCg                                                  <  2:1623362/151‑1 (MQ=255)
                                                                                               cGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCg                                                  <  2:1601739/151‑1 (MQ=255)
                                                                                               cGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCg                                                  >  1:321665/1‑151 (MQ=255)
                                                                                               cGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCg                                                  <  2:599911/151‑1 (MQ=255)
                                                                                                gTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCgg                                                 >  2:1206943/1‑151 (MQ=255)
                                                                                                 ttACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCggg                                                >  1:687307/1‑151 (MQ=255)
                                                                                                 ttACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCggg                                                >  2:77884/1‑151 (MQ=255)
                                                                                                 ttACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCggg                                                >  2:64073/1‑151 (MQ=255)
                                                                                                  tACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGCAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGACGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGt                                               >  4:282611/1‑151 (MQ=255)
                                                                                                  tACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGt                                               >  2:331269/1‑151 (MQ=255)
                                                                                                    ccGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCggg                                                >  2:1176183/1‑148 (MQ=255)
                                                                                                    ccGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTAt                                             >  2:1371725/1‑151 (MQ=255)
                                                                                                       ccATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCa                                          <  3:39793/151‑1 (MQ=255)
                                                                                                       ccACTTTCTCGCTGTTCGCTCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTc                                           <  1:738996/150‑1 (MQ=255)
                                                                                                         aTTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCAcc                                        <  3:227904/151‑1 (MQ=255)
                                                                                                         aTTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCAcc                                        <  1:1145006/151‑1 (MQ=255)
                                                                                                         aTTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCAcc                                        >  2:1173925/1‑151 (MQ=255)
                                                                                                           tttCTCGCTGTTCGCCCTGTTCAGCTCCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCg                                       <  1:403902/150‑1 (MQ=255)
                                                                                                            ttCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCgg                                      <  1:13705/150‑1 (MQ=255)
                                                                                                             tctcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGt                                     <  2:994263/150‑1 (MQ=255)
                                                                                                              ctcGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTAt                                   >  2:496989/1‑151 (MQ=255)
                                                                                                                cGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTAttt                                 <  3:476393/151‑1 (MQ=255)
                                                                                                                 gCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTa                                <  1:397643/151‑1 (MQ=255)
                                                                                                                  cTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTa                                <  2:1080213/150‑1 (MQ=255)
                                                                                                                  cTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTa                                >  1:1166374/1‑150 (MQ=255)
                                                                                                                   tGTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGCGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGGGATTGCGGGGATTCACCGGGATTTAAt                              >  2:798091/1‑151 (MQ=255)
                                                                                                                    gTTCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAt                              <  2:1523186/150‑1 (MQ=255)
                                                                                                                     ttCGCCCTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAAtt                             >  2:969941/1‑150 (MQ=255)
                                                                                                                         cccTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGatat                        >  2:50648/1‑151 (MQ=255)
                                                                                                                         cccTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGatat                        <  2:495437/151‑1 (MQ=255)
                                                                                                                          ccTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGatat                        >  4:277716/1‑150 (MQ=255)
                                                                                                                           cTGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcg                      >  2:462144/1‑151 (MQ=255)
                                                                                                                            tGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcgg                     >  1:1430473/1‑151 (MQ=255)
                                                                                                                            tGTTCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcgg                     >  3:580723/1‑151 (MQ=255)
                                                                                                                              ttCAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggcg                   >  2:310816/1‑151 (MQ=255)
                                                                                                                                cAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggcgg                  >  1:518436/1‑150 (MQ=255)
                                                                                                                                cAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATcggcgg                  <  2:815467/150‑1 (MQ=255)
                                                                                                                                cAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCggcggc                 <  1:951515/151‑1 (MQ=255)
                                                                                                                                cAGCACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCggcggc                 <  1:1536196/151‑1 (MQ=255)
                                                                                                                                   cACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGt               >  2:477828/1‑150 (MQ=255)
                                                                                                                                   cACCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCCGCGGCGt               >  1:1665741/1‑150 (MQ=255)
                                                                                                                                    aCCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTg              >  3:466916/1‑150 (MQ=255)
                                                                                                                                    aCCTGGTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGa             <  2:439923/151‑1 (MQ=255)
                                                                                                                                        ggTCCGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACgg          >  2:670487/1‑150 (MQ=255)
                                                                                                                                           ccGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGAt      <  3:231850/151‑1 (MQ=255)
                                                                                                                                           ccGGCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCACGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGa       <  2:1020804/150‑1 (MQ=255)
                                                                                                                                              gCGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGGATTTAATTGATATCGGCGGCGTGACGGCGATccc   >  1:390778/1‑151 (MQ=255)
                                                                                                                                               cGTCCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATcccc  >  2:350897/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGTTAATGCTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCATTTTCTCGCTGTTCGCCCTGTTCAGCACCTGGTCCGGCGTTCCCGTAGAAGGCTCGCTGGTGAACGTACGTATTATCGCGGTGATGTCCGGCGGGATTCTGTTTGGCCCGTGGGTAGGCATCATTACCGGCGTGATTGCGGGTATTCACCGGTATTTAATTGATATCGGCGGCGTGACGGCGATCCCC  >  NC_000913/2498716‑2499009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑