Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,519,524 T→C V383V (GTA→GTG)  gltX ← glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,5240TC99.3% 506.5 / NA 144V383V (GTA→GTG) gltXglutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (72/71);  total (73/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

TTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCTACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCGGGCCGTTA  >  NC_000913/2519376‑2519668
                                                                                                                                                    |                                                                                                                                                
ttACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCAc                                                                                                                                                >  3:557010/1‑151 (MQ=255)
  aCGGCGACACGCAGCGGCATACCAACTTTACCCATACCCCCTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCCGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCAcc                                                                                                                                               <  1:1419064/150‑1 (MQ=255)
    ggCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCgg                                                                                                                                             >  1:746259/1‑150 (MQ=255)
    ggCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGCCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCgg                                                                                                                                             >  3:141776/1‑150 (MQ=255)
     gCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGAc                                                                                                                                           <  1:1556482/151‑1 (MQ=255)
      cGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACg                                                                                                                                          >  2:297471/1‑151 (MQ=255)
       gACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGc                                                                                                                                         <  1:1645862/151‑1 (MQ=255)
         cacGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAGCGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCa                                                                                                                                        <  1:439893/150‑1 (MQ=255)
         cacGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGc                                                                                                                                         >  2:1408215/1‑149 (MQ=255)
         cacGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCa                                                                                                                                        <  1:1043615/150‑1 (MQ=255)
             cAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGt                                                                                                                                   >  4:17233/1‑151 (MQ=255)
              aGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGtt                                                                                                                                  <  2:1581419/151‑1 (MQ=255)
                cGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGtttt                                                                                                                                >  4:377711/1‑151 (MQ=255)
                 ggCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGttttt                                                                                                                               >  3:259385/1‑151 (MQ=255)
                  gCATACCAACTTTACCCATACCCACGTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGATCCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGtttttt                                                                                                                              <  1:1266758/151‑1 (MQ=255)
                   cATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTc                                                                                                                             >  1:755874/1‑151 (MQ=255)
                   cATACCAACTTGACCCATACCAACTTCCAGCACAGCCGCCGTCGCCTTAATAGCGTGATGACCGTTTTCAGCGGTGCAATCAGTAATTGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGtttttt                                                                                                                              <  1:1637692/150‑1 (MQ=255)
                       ccAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCgcgg                                                                                                                         <  2:403077/151‑1 (MQ=255)
                        cAACTTTACACATACGCTCTTCCAGCTCACCCGACATCGCCTGAATAGGGTGATGAACGTTTTCAGCGATGCAGTCAGTAATCGCGGCCAGTTTGCCACGAAACACTTCCAGCGGCTGACGCGCTCGTGGACGCAGATGTTTTTTAgcggt                                                                                                                        >  1:919767/1‑150 (MQ=255)
                          aCTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGt                                                                                                                      <  3:13379/151‑1 (MQ=255)
                            tttACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCg                                                                                                                    >  2:684468/1‑151 (MQ=255)
                            tttACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCg                                                                                                                    >  2:1637237/1‑151 (MQ=255)
                             ttACCCATACCCACTTCCAGCTCATCCGCCGTGGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCgg                                                                                                                   >  4:226768/1‑151 (MQ=255)
                             ttACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCg                                                                                                                    <  4:6173/150‑1 (MQ=255)
                              tacccatacccaCTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCgg                                                                                                                   <  2:1441085/150‑1 (MQ=255)
                                cccatacccaCTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGACGAACGTTTTCAGCGGTCCAGTCAGTACTCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGc                                                                                                                  <  1:463574/149‑1 (MQ=255)
                                 ccatacccaCTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATc                                                                                                               <  2:597748/151‑1 (MQ=255)
                                      cccaCTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACt                                                                                                          <  4:398019/151‑1 (MQ=255)
                                          cTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAg                                                                                                       <  2:975666/150‑1 (MQ=255)
                                          cTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAg                                                                                                       >  1:1511968/1‑150 (MQ=255)
                                          cTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGc                                                                                                      >  1:246226/1‑151 (MQ=255)
                                          cTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGc                                                                                                      >  2:547483/1‑151 (MQ=255)
                                            tCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGc                                                                                                      <  4:521322/149‑1 (MQ=255)
                                            tCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCa                                                                                                     >  3:190320/1‑150 (MQ=255)
                                             ccAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCaa                                                                                                    <  4:153806/150‑1 (MQ=255)
                                                   cATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTc                                                                                             >  1:1159315/1‑151 (MQ=255)
                                                      ccgccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGt                                                                                           <  2:1085600/150‑1 (MQ=255)
                                                      ccgccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGt                                                                                           >  3:115015/1‑150 (MQ=255)
                                                      ccgccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTa                                                                                          <  4:294317/151‑1 (MQ=255)
                                                      ccgccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTGCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGt                                                                                           <  1:1439720/150‑1 (MQ=255)
                                                         ccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGaa                                                                                       <  1:1698129/151‑1 (MQ=255)
                                                         ccgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGa                                                                                        >  4:305890/1‑150 (MQ=255)
                                                          cgTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGaa                                                                                       >  2:1212099/1‑150 (MQ=255)
                                                           gTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAAt                                                                                     <  1:385820/151‑1 (MQ=255)
                                                           gTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAAt                                                                                     <  1:1299628/151‑1 (MQ=255)
                                                           gTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAAt                                                                                     <  4:597391/151‑1 (MQ=255)
                                                             cGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATa                                                                                    <  1:264604/150‑1 (MQ=255)
                                                             cGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATa                                                                                    >  2:1602304/1‑150 (MQ=255)
                                                               ccTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACg                                                                                 <  1:1699934/151‑1 (MQ=255)
                                                               ccTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACg                                                                                 <  2:423214/151‑1 (MQ=255)
                                                                 tGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGc                                                                               >  1:1721033/1‑151 (MQ=255)
                                                                  gAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACgg                                                                                <  2:781992/149‑1 (MQ=255)
                                                                  gAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACgg                                                                                <  2:357234/149‑1 (MQ=255)
                                                                  gAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGc                                                                               <  1:979607/150‑1 (MQ=255)
                                                                    aTACCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGc                                                                            >  1:357327/1‑151 (MQ=255)
                                                                        cGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCtg                                                                        >  4:235535/1‑151 (MQ=255)
                                                                              gaACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCa                                                                   <  4:309984/150‑1 (MQ=255)
                                                                              gaACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCAt                                                                  >  3:197980/1‑151 (MQ=255)
                                                                                   ttttCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCtt                                                             >  2:307619/1‑151 (MQ=255)
                                                                                    tttCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCGGATGTTTTGTCGGGGCGTCGGCATCGAACTCAGCAAAATCTTCGTATAAATAACGGCGGCTCTGTGCCATCTCtt                                                             <  1:1012038/150‑1 (MQ=255)
                                                                                      tCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTc                                                           <  1:914820/150‑1 (MQ=255)
                                                                                        aGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGc                                                        <  2:4307/151‑1 (MQ=255)
                                                                                           ggTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGt                                                      >  2:629247/1‑150 (MQ=255)
                                                                                             tCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGGCGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCt                                                    <  1:599525/150‑1 (MQ=255)
                                                                                              ccAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCtt                                                   >  1:1097398/1‑150 (MQ=255)
                                                                                              ccAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTg                                                  <  2:1537452/151‑1 (MQ=255)
                                                                                                agtcagtAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCa                                                <  2:705043/151‑1 (MQ=255)
                                                                                                     gtAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGtt                                           >  2:559548/1‑151 (MQ=255)
                                                                                                      tAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTc                                          >  4:514902/1‑151 (MQ=255)
                                                                                                       aaTCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTc                                          >  1:70930/1‑150 (MQ=255)
                                                                                                       aaTCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTc                                          <  3:423172/150‑1 (MQ=255)
                                                                                                        aTCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGc                                        >  2:553222/1‑151 (MQ=255)
                                                                                                          cgcgGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGccc                                      <  1:774381/151‑1 (MQ=255)
                                                                                                            cgGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGccc                                      >  2:600179/1‑149 (MQ=255)
                                                                                                            cgGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGccc                                      >  2:681695/1‑149 (MQ=255)
                                                                                                            cgGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcag                                    <  1:374338/151‑1 (MQ=255)
                                                                                                              gCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcagc                                   >  2:890728/1‑150 (MQ=255)
                                                                                                               ccAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcagcag                                 <  1:1501173/151‑1 (MQ=255)
                                                                                                               ccAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcagcag                                 <  2:21919/151‑1 (MQ=255)
                                                                                                               ccAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcagc                                   >  2:1479456/1‑149 (MQ=255)
                                                                                                               ccAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCcagc                                   >  3:245094/1‑149 (MQ=255)
                                                                                                                 aGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGtt                               <  2:1315203/151‑1 (MQ=255)
                                                                                                                 aGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGtt                               >  2:1020554/1‑151 (MQ=255)
                                                                                                                  gTTTGTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGttt                              <  3:367002/151‑1 (MQ=255)
                                                                                                                      gTCACGACCCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCGTCGTAGAAATAACGGCAGCTATGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCACAGCAGtgtg                             <  1:1183032/148‑4 (MQ=255)
                                                                                                                      gTCACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCAcc                          >  3:239523/1‑151 (MQ=255)
                                                                                                                        cACGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCAGCGAACTCAGCAAAATCTTCGTAGAAATAACGCCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCa                         <  4:110332/150‑1 (MQ=255)
                                                                                                                         aCGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCAcc                          >  1:96749/1‑148 (MQ=255)
                                                                                                                          cGAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCAcc                          >  2:1267109/1‑147 (MQ=255)
                                                                                                                           gAACCACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCAcc                          >  1:511312/1‑146 (MQ=255)
                                                                                                                              ccACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATcagc                  >  2:1132492/1‑151 (MQ=255)
                                                                                                                               cACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATcagcc                 >  1:1347758/1‑151 (MQ=255)
                                                                                                                               cACTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGAGCCATCTCTTTCCGCGTCTTGCAGCGTTCGCCCAGCAGTTTTACCAGATcagcc                 >  1:28104/1‑151 (MQ=255)
                                                                                                                                 cTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATcagcca                <  1:72912/150‑1 (MQ=255)
                                                                                                                                 cTTCCAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATcagcca                <  1:433826/150‑1 (MQ=255)
                                                                                                                                    ccAGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTg            <  2:547850/151‑1 (MQ=255)
                                                                                                                                      aGCGGCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCg          <  1:289920/151‑1 (MQ=255)
                                                                                                                                         ggCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCGGGc       <  1:650262/151‑1 (MQ=255)
                                                                                                                                          gCTGACGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCGGGcc      <  2:1589119/151‑1 (MQ=255)
                                                                                                                                              aCGCGCCACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCCTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCGGGCCGTTa  <  1:1548690/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTACGGCGACACGCAGCGGCATACCAACTTTACCCATACCCACTTCCAGCTCATCCGCCGTCGCCTGAATAGCGTGATGAACGTTTTCAGCGGTCCAGTCAGTAATCGCGGCCAGTTTGTCACGAACCACTTCCAGCGGCTGACGCGCTACCGGACGCAGATGTTTTTTCGCGGCGTCGGCATCGAACTCAGCAAAATCTTCGTAGAAATAACGGCAGCTCTGTGCCATCTCTTTCAGCGTCTTGCAGCGTTCGCCCAGCAGTTTCACCAGATCAGCCAGCTGCGGGCCGTTA  >  NC_000913/2519376‑2519668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑