Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,602,739 A→G T308A (ACC→GCC)  hyfB → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,7390AG99.1% 412.1 / ‑6.4 111T308A (ACC→GCC) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (62/47);  total (62/49)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 111 total aligned reads displayed.

CCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTT  >  NC_000913/2602591‑2602885
                                                                                                                                                    |                                                                                                                                                  
ccTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGcc                                                                                                                                                  >  3:152506/1‑151 (MQ=255)
  tGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCg                                                                                                                                                 >  1:1694776/1‑150 (MQ=255)
     aaGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCg                                                                                                                                              >  2:96336/1‑150 (MQ=255)
     aaGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACCGCTGCTGGCCTGGAGTGCCGTCg                                                                                                                                              >  1:1057907/1‑150 (MQ=255)
      aGTAGCGATGGATCTGCTGGCGCACACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGGGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCg                                                                                                                                              >  2:41718/1‑149 (MQ=255)
       gTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGaaa                                                                                                                                           <  1:179275/151‑1 (MQ=255)
        tAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGaaa                                                                                                                                           >  1:475321/1‑150 (MQ=255)
           cGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGt                                                                                                                                       <  1:677050/151‑1 (MQ=255)
            gATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACg                                                                                                                                        >  2:147181/1‑149 (MQ=255)
             aTGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCg                                                                                                                                     <  2:1650864/151‑1 (MQ=255)
               ggATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGc                                                                                                                                   <  3:42459/151‑1 (MQ=255)
                gATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGc                                                                                                                                   >  2:1198470/1‑150 (MQ=255)
                 aTCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCat                                                                                                                                 >  2:399023/1‑151 (MQ=255)
                 aTCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCat                                                                                                                                 >  1:469724/1‑151 (MQ=255)
                 aTCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCat                                                                                                                                 >  1:1078439/1‑151 (MQ=255)
                    tgctgGCGCAAACGGGTTTGCCTCTGTGGAGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGGCGAAAACGGCGGCatta                                                                                                                               >  4:366746/1‑150 (MQ=255)
                     gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCattatt                                                                                                                             >  1:1168437/1‑151 (MQ=255)
                     gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCattat                                                                                                                              <  2:918574/150‑1 (MQ=255)
                     gctgGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCatt                                                                                                                                <  1:511793/148‑1 (MQ=255)
                        ggCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTAtttt                                                                                                                           <  2:32020/150‑1 (MQ=255)
                        ggCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTAtttt                                                                                                                           <  1:183779/150‑1 (MQ=255)
                           gcAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTg                                                                                                                       <  2:1386594/151‑1 (MQ=255)
                            cAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTgg                                                                                                                      <  2:1302962/151‑1 (MQ=255)
                             aaaCGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGc                                                                                                                     >  2:1468279/1‑151 (MQ=255)
                             aaaCGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGc                                                                                                                     >  2:763777/1‑151 (MQ=255)
                              aaCGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCa                                                                                                                    <  2:1295932/151‑1 (MQ=255)
                                 gggTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGt                                                                                                                  <  3:355337/150‑1 (MQ=255)
                                  ggTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCg                                                                                                                <  1:3709/151‑1 (MQ=255)
                                  ggTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCg                                                                                                                <  1:1144124/151‑1 (MQ=255)
                                   gTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCgg                                                                                                               >  4:206799/1‑151 (MQ=255)
                                   gTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCgg                                                                                                               >  2:614129/1‑151 (MQ=255)
                                    tttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCgg                                                                                                               >  2:1373455/1‑150 (MQ=255)
                                    tttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCgg                                                                                                               >  1:1534703/1‑150 (MQ=255)
                                     ttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAATCGGCTGCTGGCCTGGAGTGCCGTCGAAACCGTCGGCATTATTTTGCTGGCAGTCGgt                                                                                                              <  1:379135/150‑1 (MQ=255)
                                     ttGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtg                                                                                                             >  1:583067/1‑151 (MQ=255)
                                      tGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGgtgt                                                                                                            >  3:215696/1‑151 (MQ=255)
                                       gCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGtgtg                                                                                                           <  2:830011/151‑1 (MQ=255)
                                        ccTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTgg                                                                                                          <  2:1047288/151‑1 (MQ=255)
                                        ccTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTgg                                                                                                          >  1:255132/1‑151 (MQ=255)
                                         ctctGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTgg                                                                                                          >  1:1415629/1‑150 (MQ=255)
                                            tgtgGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCgct                                                                                                      >  1:37177/1‑149 (MQ=255)
                                               ggtggGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTACTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggt                                                                                                   <  2:829615/151‑1 (MQ=255)
                                                  ggggCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATggtcg                                                                                                 <  2:1587580/150‑1 (MQ=255)
                                                   gggAATTCCGGTGATGGCGATCGGCGCAATCTCCGCGCTCACGGGAGTGCTATATGCGCTGGCGGAACAGGATATCAAATGGCTGATGGGCTGGAGTGCCGTCGAAAACGTCGGTATTATTTTGCTGGCAGTGGGTGTGGCGAGggtgg                                                                                                 >  1:1612808/1‑147 (MQ=255)
                                                         tCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTc                                                                                          >  2:1368889/1‑150 (MQ=255)
                                                              tGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGc                                                                                     >  2:928948/1‑150 (MQ=255)
                                                               gATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCa                                                                                    <  2:168320/150‑1 (MQ=255)
                                                                  ggCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAc                                                                                >  3:276843/1‑151 (MQ=255)
                                                                   gCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGAcc                                                                               >  2:1457363/1‑151 (MQ=255)
                                                                     gATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCg                                                                             >  2:475616/1‑151 (MQ=255)
                                                                       tCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCgct                                                                           >  1:1479477/1‑151 (MQ=255)
                                                                          gcgcAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCgctgct                                                                        >  2:418064/1‑151 (MQ=255)
                                                                              aaTCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCAcc                                                                    >  2:1229952/1‑151 (MQ=255)
                                                                              aaTCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCAcc                                                                    >  2:1736491/1‑151 (MQ=255)
                                                                                         tCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACt                                                         >  2:1111730/1‑151 (MQ=255)
                                                                                          ccTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGAc                                                          <  2:1457033/149‑1 (MQ=255)
                                                                                          ccTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACt                                                         <  2:565064/150‑1 (MQ=255)
                                                                                            tGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCt                                                      >  4:378148/1‑151 (MQ=255)
                                                                                             gggCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCt                                                      <  2:979450/150‑1 (MQ=255)
                                                                                             gggCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTc                                                     <  1:52298/151‑1 (MQ=255)
                                                                                             gggCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTc                                                     <  2:1612079/151‑1 (MQ=255)
                                                                                             gggCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGACCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCTATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTc                                                     <  1:1212550/151‑1 (MQ=255)
                                                                                               gCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCgg                                                   >  2:439761/1‑151 (MQ=255)
                                                                                                cGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGgc                                                  <  1:1484363/151‑1 (MQ=255)
                                                                                                   gCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCa                                               <  1:94029/151‑1 (MQ=255)
                                                                                                   gCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCa                                               <  1:1183675/151‑1 (MQ=255)
                                                                                                      atatGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTg                                            <  2:1065375/151‑1 (MQ=255)
                                                                                                          gcgcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCTGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGttt                                         <  2:622501/150‑1 (MQ=255)
                                                                                                          gcgcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGttt                                         <  2:1286364/150‑1 (MQ=255)
                                                                                                          gcgcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTc                                        >  3:284323/1‑151 (MQ=255)
                                                                                                           cgcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCa                                       <  2:469798/151‑1 (MQ=255)
                                                                                                            gcTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCAt                                      <  2:26988/151‑1 (MQ=255)
                                                                                                              tGGCGGAACAGGATATCAAAGGGCTGCTGGCCTGGCGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCAACGTGGTTGGACTGCTCGGCGCACTGTTTCATCt                                    >  1:71485/1‑151 (MQ=255)
                                                                                                              tGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCt                                    >  2:569467/1‑151 (MQ=255)
                                                                                                                    aaCTGGATATCAAACGGCTGCTGGCCTGGAGTTCCGTCGAAATCGTCGGCATTATTTTGCTGGCAGTCGTTGTGTCGATGGTCGGTCTGTCCCTCCCCGACCCGCTGCTCACCGTGCTTCGACTGCTCGGCGCACTGTTTCATCTGCTct                               <  4:140670/150‑2 (MQ=255)
                                                                                                                     aCAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAAc                             <  1:136393/151‑1 (MQ=255)
                                                                                                                      cAGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAAcc                            >  1:1070681/1‑151 (MQ=255)
                                                                                                                       aGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAAcc                            >  1:1539086/1‑150 (MQ=255)
                                                                                                                       aGGATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCa                           <  2:784725/151‑1 (MQ=255)
                                                                                                                        ggATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCAt                          <  1:1205989/151‑1 (MQ=255)
                                                                                                                         gATATCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATg                         <  1:1248604/151‑1 (MQ=255)
                                                                                                                            atCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATgcgc                      >  3:588208/1‑151 (MQ=255)
                                                                                                                            atCAAACGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATgcgc                      <  3:250512/151‑1 (MQ=255)
                                                                                                                               aaaCGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGt                   >  3:210804/1‑151 (MQ=255)
                                                                                                                                aaCGGCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGtt                  <  2:834129/151‑1 (MQ=255)
                                                                                                                                  tggCTGCTGGCCTAGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTc                 >  4:368100/2‑150 (MQ=255)
                                                                                                                                   ggCTGCTGGCCTGGAGTGCCGTCGGAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGCTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGTGCACTGTTTCATCTGCTCAACCATGCGCTGTTc                 >  1:1494159/1‑149 (MQ=255)
                                                                                                                                   ggCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCaa               <  2:1028628/151‑1 (MQ=255)
                                                                                                                                   ggCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCa                >  2:1412549/1‑150 (MQ=255)
                                                                                                                                   ggCTGCTGGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCa                >  3:584343/1‑150 (MQ=255)
                                                                                                                                      tgctgGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAgg            >  2:17019/1‑151 (MQ=255)
                                                                                                                                       gctgGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAggg           <  1:1652534/151‑1 (MQ=255)
                                                                                                                                         tgGCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgct         >  3:528009/1‑151 (MQ=255)
                                                                                                                                           gCCTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgctgc       >  3:72546/1‑151 (MQ=255)
                                                                                                                                            ccTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgctgct      >  2:1131126/1‑151 (MQ=255)
                                                                                                                                             cTGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGgctgct      >  1:293660/1‑150 (MQ=255)
                                                                                                                                              tGGAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAt    >  1:834500/1‑151 (MQ=255)
                                                                                                                                               ggAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAtt   >  1:396630/1‑151 (MQ=255)
                                                                                                                                               ggAGTGCCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAtt   >  2:189108/1‑151 (MQ=255)
                                                                                                                                                gAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGCCGGTGTGGCGATGGTCGGTCTTTCACTCCACGACCCGCTGCTCACCGTGGTTCGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTAtta  <  1:1142521/151‑2 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCTCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTATATGCGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCAGTCGGTGTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTT  >  NC_000913/2602591‑2602885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑