Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,1500GA100.0% 483.0 / NA 119A59V (GCT→GTT) pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (64/55);  total (64/55)

Only 100 of 119 total aligned reads displayed.

GTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAG  >  NC_000913/2647003‑2647296
                                                                                                                                                   |                                                                                                                                                  
gTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACAt                                                                                                                                                 <  2:1079545/151‑1 (MQ=255)
  gagaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCg                                                                                                                                               >  2:126673/1‑151 (MQ=255)
   agaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCgg                                                                                                                                              >  1:322984/1‑151 (MQ=255)
      tCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCg                                                                                                                                            >  4:228126/1‑150 (MQ=255)
        ttGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGa                                                                                                                                           >  1:1715724/1‑149 (MQ=255)
         tGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGaa                                                                                                                                          <  3:448604/149‑1 (MQ=255)
          gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAgcg                                                                                                                                       >  4:434077/1‑151 (MQ=255)
          gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAgc                                                                                                                                        <  1:405829/150‑1 (MQ=255)
               gctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGcc                                                                                                                                     >  2:1574988/1‑148 (MQ=255)
                  gcgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCagag                                                                                                                                 <  2:317365/149‑1 (MQ=255)
                   cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAgc                                                                                                                                <  2:1291548/149‑1 (MQ=255)
                      gcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGt                                                                                                                           <  1:512729/151‑1 (MQ=255)
                      gcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGt                                                                                                                           <  3:562528/151‑1 (MQ=255)
                       cgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGt                                                                                                                           >  3:319282/1‑150 (MQ=255)
                          cACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTAcc                                                                                                                        >  3:33476/1‑150 (MQ=255)
                           aCCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCAt                                                                                                                      >  3:90225/1‑151 (MQ=255)
                             cGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCAt                                                                                                                      <  4:386182/149‑1 (MQ=255)
                             cGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATcc                                                                                                                    >  4:522142/1‑151 (MQ=255)
                             cGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATcc                                                                                                                    >  2:754964/1‑151 (MQ=255)
                                     gATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCAc                                                                                                            <  4:532121/151‑1 (MQ=255)
                                      aTTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACg                                                                                                           <  2:559360/151‑1 (MQ=255)
                                         cACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAcc                                                                                                        <  1:927808/151‑1 (MQ=255)
                                          aCCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAcc                                                                                                        >  1:978992/1‑150 (MQ=255)
                                            ccccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACt                                                                                                     >  2:112961/1‑151 (MQ=255)
                                             cccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTc                                                                                                    >  3:275790/1‑151 (MQ=255)
                                               cGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGt                                                                                                  <  2:51482/151‑1 (MQ=255)
                                               cGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGt                                                                                                  <  2:108939/151‑1 (MQ=255)
                                                 gATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGc                                                                                                >  2:1147628/1‑151 (MQ=255)
                                                       tCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGAtt                                                                                          >  1:588566/1‑151 (MQ=255)
                                                        ccAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCg                                                                                                   >  2:1169516/1‑141 (MQ=255)
                                                        ccAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCg                                                                                                   <  1:1169381/141‑1 (MQ=255)
                                                         cAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTg                                                                                         >  1:687580/1‑150 (MQ=255)
                                                         cAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTg                                                                                         >  1:1397152/1‑150 (MQ=255)
                                                            aaCCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACtt                                                                                     >  4:38953/1‑151 (MQ=255)
                                                             aCCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTc                                                                                    >  1:1586334/1‑151 (MQ=255)
                                                              ccAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTc                                                                                    >  2:819514/1‑150 (MQ=255)
                                                              ccAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCa                                                                                   <  2:1127503/151‑1 (MQ=255)
                                                                aGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATg                                                                                 <  2:214787/151‑1 (MQ=255)
                                                                  cGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGc                                                                                >  2:811062/1‑150 (MQ=255)
                                                                   gATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGc                                                                                >  3:173789/1‑149 (MQ=255)
                                                                    aTCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGc                                                                             >  2:500878/1‑151 (MQ=255)
                                                                       ttCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCgg                                                                           >  1:1469941/1‑150 (MQ=255)
                                                                        tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCa                                                                               <  1:1213422/145‑1 (MQ=255)
                                                                        tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGc                                                                             <  2:793454/147‑1 (MQ=255)
                                                                         cATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTag                                                                        <  3:228145/151‑1 (MQ=255)
                                                                            aaTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAgg                                                                     >  1:1676765/1‑151 (MQ=255)
                                                                            aaTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAgg                                                                     <  2:49669/151‑1 (MQ=255)
                                                                             aTTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGc                                                                    <  2:1301010/151‑1 (MQ=255)
                                                                              ttGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  1:1192217/1‑151 (MQ=255)
                                                                              ttGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGcc                                                                   >  1:597796/1‑151 (MQ=255)
                                                                                 aTCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCaga                                                                <  2:1064227/151‑1 (MQ=255)
                                                                                  tCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCag                                                                 >  2:26020/1‑149 (MQ=255)
                                                                                   cAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGt                                                              >  3:311852/1‑151 (MQ=255)
                                                                                    aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                             <  1:454317/151‑1 (MQ=255)
                                                                                       gcTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTAt                                                          >  2:1229934/1‑151 (MQ=255)
                                                                                       gcTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTAt                                                          <  2:990793/151‑1 (MQ=255)
                                                                                       gcTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTAt                                                          >  3:567460/1‑151 (MQ=255)
                                                                                            aaGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGttt                                                            >  2:482157/1‑144 (MQ=255)
                                                                                            aaGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGttt                                                            <  1:482080/144‑1 (MQ=255)
                                                                                               gTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTccc                                                  >  4:265396/1‑151 (MQ=255)
                                                                                                 aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                                <  1:497136/151‑1 (MQ=255)
                                                                                                 aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                                <  2:1310911/151‑1 (MQ=255)
                                                                                                 aaCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCa                                                <  3:233561/151‑1 (MQ=255)
                                                                                                  aCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCAc                                               >  2:1388217/1‑151 (MQ=255)
                                                                                                  aCGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCAc                                               <  2:747165/151‑1 (MQ=255)
                                                                                                      ggAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCt                                            <  2:11134/150‑1 (MQ=255)
                                                                                                        agaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                         <  1:1028519/151‑1 (MQ=255)
                                                                                                          aaaCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAg                                       <  1:1391939/151‑1 (MQ=255)
                                                                                                            aCATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGAGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGa                                      >  3:463947/1‑150 (MQ=255)
                                                                                                                 ttCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                >  1:516021/1‑151 (MQ=255)
                                                                                                                  tCGATTGTTACCGGATGACGCCAGATGCCGACAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCAATCGGGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                >  1:615650/1‑150 (MQ=255)
                                                                                                                  tCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaaaa                                   <  2:552283/147‑1 (MQ=255)
                                                                                                                   cGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGga                               <  2:251342/150‑1 (MQ=255)
                                                                                                                    gATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                >  3:125707/1‑148 (MQ=255)
                                                                                                                    gATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                                >  4:438278/1‑148 (MQ=255)
                                                                                                                    gATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGgaga                             <  1:1574828/151‑1 (MQ=255)
                                                                                                                     aTTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGgaga                             >  4:478930/1‑150 (MQ=255)
                                                                                                                        gTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAgg                         <  3:478001/151‑1 (MQ=255)
                                                                                                                         ttACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAggg                        >  4:477825/1‑151 (MQ=255)
                                                                                                                          tACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaaa                                    >  4:481592/1‑138 (MQ=255)
                                                                                                                          tACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaaa                                    <  3:481610/138‑1 (MQ=255)
                                                                                                                              ggATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCgg                   <  1:1192595/151‑1 (MQ=255)
                                                                                                                                  aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGGCTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc               <  2:1561951/151‑1 (MQ=255)
                                                                                                                                  aaCGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGc               >  4:445523/1‑151 (MQ=255)
                                                                                                                                     gCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCaa            <  1:131026/151‑1 (MQ=255)
                                                                                                                                      ccAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAAc           >  2:1442617/1‑151 (MQ=255)
                                                                                                                                      ccAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAAc           <  2:59714/151‑1 (MQ=255)
                                                                                                                                      ccAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAAc           <  1:1326121/151‑1 (MQ=255)
                                                                                                                                       cAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACg          >  2:1302582/1‑151 (MQ=255)
                                                                                                                                        aGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGt         >  1:654730/1‑151 (MQ=255)
                                                                                                                                        aGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGt         >  3:174649/1‑151 (MQ=255)
                                                                                                                                          aTGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGtt        >  2:419785/1‑150 (MQ=255)
                                                                                                                                          aTGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTa       <  1:1146107/151‑1 (MQ=255)
                                                                                                                                          aTGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTa       <  3:391/151‑1 (MQ=255)
                                                                                                                                           tGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTAt      >  2:458272/1‑151 (MQ=255)
                                                                                                                                             ccGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATcc    >  2:575090/1‑151 (MQ=255)
                                                                                                                                             ccGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATcc    >  2:655038/1‑151 (MQ=255)
                                                                                                                                              cGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATcc    >  2:193651/1‑150 (MQ=255)
                                                                                                                                              cGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCca   <  2:1365491/151‑1 (MQ=255)
                                                                                                                                               gTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCcag  <  3:159403/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAG  >  NC_000913/2647003‑2647296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑