Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,140 T→C L53L (TTG→CTG)  rnlB → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,1400TC98.6% 537.3 / ‑1.8 141L53L (TTG→CTG) rnlBCP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (79/60);  total (80/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 142 total aligned reads displayed.

ATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCAT  >  NC_000913/2766993‑2767286
                                                                                                                                                   |                                                                                                                                                  
aTCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCtgt                                                                                                                                                 <  2:3431/151‑1 (MQ=255)
aTCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCtgt                                                                                                                                                 >  2:495339/1‑151 (MQ=255)
     cGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCt                                                                                                                                            >  2:223878/1‑151 (MQ=255)
      ggAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCt                                                                                                                                            >  4:356049/1‑150 (MQ=255)
        aaTTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAAc                                                                                                                                         <  2:1612314/151‑1 (MQ=255)
            aaCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTc                                                                                                                                     <  1:632346/151‑1 (MQ=255)
            aaCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTc                                                                                                                                     >  2:363496/1‑151 (MQ=255)
             aCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTcc                                                                                                                                    >  2:1008076/1‑151 (MQ=255)
                tctcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTcc                                                                                                                                    >  1:1112944/1‑148 (MQ=255)
                 ctcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCag                                                                                                                                  >  2:1184276/1‑149 (MQ=255)
                   cAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGt                                                                                                                               <  3:177805/150‑1 (MQ=255)
                   cAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTg                                                                                                                              >  3:198222/1‑151 (MQ=255)
                   cAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTg                                                                                                                              >  1:1270426/1‑151 (MQ=255)
                    aGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTgg                                                                                                                             <  2:283522/151‑1 (MQ=255)
                        gCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAAcc                                                                                                                         <  3:195326/151‑1 (MQ=255)
                         ctctAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCg                                                                                                                        >  2:1723518/1‑151 (MQ=255)
                            tAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCtt                                                                                                                     >  3:112441/1‑151 (MQ=255)
                             aaaaGCTGTCGTTATGGCGACCGGCTTCGAGAATCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGGCCTCCTGTGCGCTAACGGTCCCGAGTGGAACCGCt                                                                                                                      <  1:1264668/149‑1 (MQ=255)
                             aaaaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCtt                                                                                                                     <  1:376251/150‑1 (MQ=255)
                                aGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGtt                                                                                                                 <  1:142703/151‑1 (MQ=255)
                                   tGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACg                                                                                                              >  4:293178/1‑151 (MQ=255)
                                          aTGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACTTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTCCGCTGgaa                                                                                                        >  2:1173759/1‑150 (MQ=255)
                                          aTGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaa                                                                                                       <  2:1553084/151‑1 (MQ=255)
                                              cGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatga                                                                                                    >  4:158993/1‑150 (MQ=255)
                                              cGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatga                                                                                                    >  2:766490/1‑150 (MQ=255)
                                              cGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatg                                                                                                     >  2:1024355/1‑149 (MQ=255)
                                               gACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatgaa                                                                                                   >  1:1598558/1‑150 (MQ=255)
                                                aCCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatgaaat                                                                                                 <  2:166177/151‑1 (MQ=255)
                                                 ccGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGgaaatgaaat                                                                                                 >  2:934576/1‑150 (MQ=255)
                                                    gCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATg                                                                                              <  1:689242/150‑1 (MQ=255)
                                                    gCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATgg                                                                                             >  3:524662/1‑151 (MQ=255)
                                                       tCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGcc                                                                                           <  3:594862/150‑1 (MQ=255)
                                                       tCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGc                                                                                            <  4:588595/149‑1 (MQ=255)
                                                        cGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                         >  3:369956/1‑151 (MQ=255)
                                                         gagaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                         >  3:444567/1‑150 (MQ=255)
                                                         gagaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                         >  4:161563/1‑150 (MQ=255)
                                                         gagaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCTGa                                                                                        <  1:1427908/151‑1 (MQ=255)
                                                         gagaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGa                                                                                        <  1:1349073/151‑1 (MQ=255)
                                                          agaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGa                                                                                        >  1:1014271/1‑150 (MQ=255)
                                                            aaCCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCa                                                                                     <  1:373643/151‑1 (MQ=255)
                                                              cccTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATg                                                                                   >  3:374388/1‑151 (MQ=255)
                                                               ccTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCGACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGc                                                                                  <  1:1672194/151‑1 (MQ=255)
                                                                cTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCt                                                                                 >  1:874375/1‑151 (MQ=255)
                                                                      ccTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATa                                                                            >  3:594620/1‑150 (MQ=255)
                                                                      ccTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATAc                                                                           <  2:828825/151‑1 (MQ=255)
                                                                           gTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGcc                                                                       >  1:395339/1‑150 (MQ=255)
                                                                           gTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGcc                                                                       >  2:210010/1‑150 (MQ=255)
                                                                            ttAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCa                                                                      >  2:1141694/1‑150 (MQ=255)
                                                                             tAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCaa                                                                     >  2:304897/1‑150 (MQ=255)
                                                                              aaTGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCaaaa                                                                   <  2:1440582/151‑1 (MQ=255)
                                                                              aaTGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCaaa                                                                    >  1:577108/1‑150 (MQ=255)
                                                                               aTGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAat                                                                  <  2:880381/151‑1 (MQ=255)
                                                                                tGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATTCTTTTTGCCCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATCGCCGGATCATGCTGGATACGGCCAAAatt                                                                 >  1:9929/1‑151 (MQ=255)
                                                                                 gAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAatta                                                                <  1:1513092/151‑1 (MQ=255)
                                                                                 gAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAatt                                                                 <  1:363443/150‑1 (MQ=255)
                                                                                    aTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGGGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtg                                                             <  1:1081072/151‑1 (MQ=255)
                                                                                      tGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgat                                                           >  2:558501/1‑151 (MQ=255)
                                                                                      tGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgat                                                           >  2:958405/1‑151 (MQ=255)
                                                                                       gagaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                          <  1:180194/151‑1 (MQ=255)
                                                                                       gagaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                          <  4:473790/151‑1 (MQ=255)
                                                                                       gagaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                          <  2:836825/151‑1 (MQ=255)
                                                                                        agaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatga                                                         <  1:1738872/151‑1 (MQ=255)
                                                                                        agaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                          >  1:980244/1‑150 (MQ=255)
                                                                                         gaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAg                                                        <  2:992435/151‑1 (MQ=255)
                                                                                             aaaCTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAgg                                                    >  2:879922/1‑151 (MQ=255)
                                                                                             aaaCTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAg                                                     >  2:588711/1‑150 (MQ=255)
                                                                                              aaCTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGa                                                   <  2:58744/151‑1 (MQ=255)
                                                                                               aCTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGAc                                                  <  2:980490/151‑1 (MQ=255)
                                                                                                       cccTACTGGGTAGTGAGACTACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCACAATTATTGATGAGCAGAACGATCCGa                                           >  4:291279/1‑150 (MQ=255)
                                                                                                       cccTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGac                                          >  1:1605429/1‑151 (MQ=255)
                                                                                                         cTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGacac                                        >  1:1550927/1‑151 (MQ=255)
                                                                                                           aCTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACat                                      <  1:552529/151‑1 (MQ=255)
                                                                                                            cTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACat                                      >  2:1520506/1‑150 (MQ=255)
                                                                                                              gggTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACatat                                    <  3:159014/150‑1 (MQ=255)
                                                                                                                 tAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTg                                >  1:309945/1‑151 (MQ=255)
                                                                                                                  aGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTg                                >  2:1524240/1‑150 (MQ=255)
                                                                                                                  aGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTg                                >  2:582710/1‑150 (MQ=255)
                                                                                                                        aCCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAAc                         <  2:1270571/151‑1 (MQ=255)
                                                                                                                          cACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTg                       >  2:836520/1‑151 (MQ=255)
                                                                                                                          cACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTg                       <  1:605011/151‑1 (MQ=255)
                                                                                                                             tGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACt                    >  2:118451/1‑151 (MQ=255)
                                                                                                                             tGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACt                    >  2:101595/1‑151 (MQ=255)
                                                                                                                             tGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACt                    >  3:517082/1‑151 (MQ=255)
                                                                                                                               gTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACttt                  <  2:1153187/151‑1 (MQ=255)
                                                                                                                                tGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt                 >  4:270849/1‑151 (MQ=255)
                                                                                                                                tGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt                 >  2:846554/1‑151 (MQ=255)
                                                                                                                                 gAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt                 >  3:472437/1‑150 (MQ=255)
                                                                                                                                 gAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt                 >  2:208797/1‑150 (MQ=255)
                                                                                                                                 gAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTttta                <  2:443293/151‑1 (MQ=255)
                                                                                                                                  aaaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttac               >  1:1136282/1‑151 (MQ=255)
                                                                                                                                  aaaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttac               <  2:1429476/151‑1 (MQ=255)
                                                                                                                                  aaaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttac               <  2:554044/151‑1 (MQ=255)
                                                                                                                                   aaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttact              >  4:577317/1‑151 (MQ=255)
                                                                                                                                   aaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttact              >  1:1129446/1‑151 (MQ=255)
                                                                                                                                   aaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttact              >  2:373140/1‑151 (MQ=255)
                                                                                                                                      ccTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttacttt            <  3:223334/150‑1 (MQ=255)
                                                                                                                                          tttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGt        <  2:1275486/150‑1 (MQ=255)
                                                                                                                                           ttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTa       <  2:1604270/150‑1 (MQ=255)
                                                                                                                                             gACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAatc    >  1:755669/1‑151 (MQ=255)
                                                                                                                                               cctcctGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAatcat  <  3:522067/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCAT  >  NC_000913/2766993‑2767286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: