Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,780,330 G→A G799G (GGC→GGT ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,780,3300GA99.3% 518.5 / NA 135G799G (GGC→GGTypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (75/59);  minor base C (0/1);  total (75/60)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCT  >  NC_000913/2780183‑2780476
                                                                                                                                                   |                                                                                                                                                  
   ttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACATTTGTTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCat                                                                                                                                               <  1:1721615/150‑1 (MQ=255)
   ttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCat                                                                                                                                               >  1:73547/1‑150 (MQ=255)
     ttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                            >  2:1063171/1‑151 (MQ=255)
      tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                            >  1:895102/1‑150 (MQ=255)
      tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATCCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCTACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                            <  1:1471266/150‑1 (MQ=255)
        ttGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGa                                                                                                                                          <  4:413883/150‑1 (MQ=255)
        ttGCCTCTCCGTGAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAGTg                                                                                                                                           <  1:1605064/149‑1 (MQ=255)
          gCCTCTCCGTTAGGGGAACCTGTTTGTATTCCGCCGTCTTTAATTGTTGTCTCATTCGCCACCCCCTCAACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCt                                                                                                                                       <  1:602686/151‑1 (MQ=255)
           ccTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCtg                                                                                                                                      >  3:344134/1‑151 (MQ=255)
              ctcCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTgtgt                                                                                                                                   >  1:519820/1‑151 (MQ=255)
               tcCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGtt                                                                                                                                  >  2:1122606/1‑151 (MQ=255)
                ccGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCtgtg                                                                                                                                    <  1:1040309/148‑1 (MQ=255)
                ccGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGttt                                                                                                                                 >  2:90442/1‑151 (MQ=255)
                   ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGtttt                                                                                                                                <  1:1066566/149‑1 (MQ=255)
                   ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCTTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGCCGCAGGGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                              >  4:199850/1‑151 (MQ=255)
                   ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                              >  1:799890/1‑151 (MQ=255)
                    tAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                              <  3:329041/150‑1 (MQ=255)
                    tAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTc                                                                                                                             >  2:607252/1‑151 (MQ=255)
                    tAGCTGAAACTGCTTGTATTCCGTCGTCGTTAATTGCTGGGTCATTCGCCACACCCTGGACAATTTCTTCACCGCCGGTGTGCGTCGTGCCGGACGCTACGCCTTTTGTTTTGACGATCTCCCGACCACCCATATTGACCTGTGGTTTGTc                                                                                                                             >  1:844075/1‑151 (MQ=255)
                          aaaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCaga                                                                                                                          >  3:185047/1‑148 (MQ=255)
                                 ttGTATTCCGCCGTCGTTAGTTGGTGTCTCATGCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGGCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCGCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGGGTCt                                                                                                                >  1:619709/1‑151 (MQ=255)
                                   gTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTg                                                                                                               <  2:51052/150‑1 (MQ=255)
                                    tATTCGGACGGCGTTAATTGTTGTATCATTGGCCACACTATCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGAc                                                                                                             >  1:1358358/1‑151 (MQ=255)
                                    tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGAc                                                                                                             >  1:707743/1‑151 (MQ=255)
                                     aTTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                                                            >  3:444234/1‑151 (MQ=255)
                                      ttCCGCCGTCGTTAATTGTTTTCTCATTCCCCACCCCCCCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGa                                                                                                              <  2:674020/148‑1 (MQ=255)
                                       tCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTcc                                                                                                          >  1:504933/1‑151 (MQ=255)
                                        ccgccgTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTc                                                                                                           <  1:210126/149‑1 (MQ=255)
                                          gccgTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACg                                                                                                       >  3:4524/1‑151 (MQ=255)
                                          gccgTCGTTAATTGTTGTCTCATTAGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACg                                                                                                       >  1:247066/1‑151 (MQ=255)
                                            cgtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGt                                                                                                     >  1:1027233/1‑151 (MQ=255)
                                            cgtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGt                                                                                                     >  3:352523/1‑151 (MQ=255)
                                             gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTTTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                                    <  2:444267/151‑1 (MQ=255)
                                              tcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                                    >  4:264614/1‑150 (MQ=255)
                                                gtTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                                    <  1:209671/148‑1 (MQ=255)
                                                gtTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTaa                                                                                                  <  2:1315707/150‑1 (MQ=255)
                                                 ttAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTaa                                                                                                  <  1:1269908/149‑1 (MQ=255)
                                                 ttAATTGTTGCCCTATCCGCCGCTCCCTCGACAATTTGTATTCCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCGCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCCCGGtg                                                                                                    <  1:1492254/147‑2 (MQ=255)
                                                     ttgttgTCTCTTTCGCCGCCCCCTCGACATTTTGTTCTCCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGATTCCACGGTTa                                                                                                   <  1:1459716/144‑1 (MQ=255)
                                                       gttgtCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccg                                                                                           <  3:283402/150‑1 (MQ=255)
                                                       gttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccgc                                                                                          >  1:414906/1‑151 (MQ=255)
                                                        ttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACgccgcc                                                                                         >  3:378261/1‑151 (MQ=255)
                                                           tctcATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAtt                                                                                      >  3:271054/1‑151 (MQ=255)
                                                            ctcATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAttt                                                                                     >  1:1264688/1‑151 (MQ=255)
                                                               aTTCGCCCCCCCCTCGACAATTGGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGcc                                                                                  <  1:1449810/151‑1 (MQ=255)
                                                               aTTCGCCCCACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGc                                                                                   <  2:1308676/150‑1 (MQ=255)
                                                               aTTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGcc                                                                                  >  1:1202217/1‑151 (MQ=255)
                                                               aTTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGcc                                                                                  >  2:120568/1‑151 (MQ=255)
                                                                ttCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGcc                                                                                  >  1:1377340/1‑150 (MQ=255)
                                                                ttCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGAAGTGGCATTTGTTTTGACGAGCTCCCGTCCACCCATATTGAACTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAttt                                                                                     >  1:1700308/1‑147 (MQ=255)
                                                                 tCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGCCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTg                                                                                    >  1:1704448/1‑147 (MQ=255)
                                                                  cGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCca                                                                                 <  1:750589/149‑1 (MQ=255)
                                                                    ccACCCCCTCGACAATTTTTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagca                                                                              <  1:1037807/150‑1 (MQ=255)
                                                                    ccACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagca                                                                              <  3:98798/150‑1 (MQ=255)
                                                                       cccccTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGat                                                                          <  1:376186/149‑1 (MQ=255)
                                                                       cacCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGat                                                                          >  2:193565/1‑151 (MQ=255)
                                                                       cacCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGat                                                                          <  2:159507/151‑1 (MQ=255)
                                                                           cTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTg                                                                      >  2:312303/1‑151 (MQ=255)
                                                                            tCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGt                                                                     >  1:1025899/1‑151 (MQ=255)
                                                                            tCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGt                                                                     >  1:1262888/1‑151 (MQ=255)
                                                                             cGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGGCGCCATTTGCCAGCAGGATATTGtt                                                                    <  2:759918/151‑1 (MQ=255)
                                                                             cGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                                                                    >  2:424673/1‑151 (MQ=255)
                                                                               aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGt                                                                     <  1:577645/148‑1 (MQ=255)
                                                                               aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcg                                                                  <  1:1008860/151‑1 (MQ=255)
                                                                               aCAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTc                                                                   <  4:387861/150‑1 (MQ=255)
                                                                                  aTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                                                               >  2:1060200/1‑151 (MQ=255)
                                                                                   tttGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                                                               <  1:355163/150‑1 (MQ=255)
                                                                                    tttttCCCCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTTTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgcc                                                                <  1:1584794/145‑1 (MQ=255)
                                                                                       ttCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAccc                                                          >  1:1638684/1‑151 (MQ=255)
                                                                                        tCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCt                                                         >  4:220459/1‑151 (MQ=255)
                                                                                         cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCt                                                         >  3:216684/1‑150 (MQ=255)
                                                                                         cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCt                                                         <  2:792373/150‑1 (MQ=255)
                                                                                         cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTg                                                        <  2:519901/151‑1 (MQ=255)
                                                                                            cgccgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTc                                                     >  1:408491/1‑151 (MQ=255)
                                                                                             gccgGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCg                                                    >  1:1284641/1‑151 (MQ=255)
                                                                                                ggTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATg                                                 <  4:1307/151‑1 (MQ=255)
                                                                                                ggTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATg                                                 <  2:295361/151‑1 (MQ=255)
                                                                                                 gTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCCCCCATATTGACCTGTGTTTTGTCAGAAGAGGTTTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGa                                                   <  1:1405194/148‑1 (MQ=255)
                                                                                                 gTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGc                                                >  4:132765/1‑151 (MQ=255)
                                                                                                     gCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGaa                                            <  1:1546734/151‑1 (MQ=255)
                                                                                                      cgtcgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAc                                           >  2:899116/1‑151 (MQ=255)
                                                                                                       gtcgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCCTATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATTCTGAAc                                           >  1:165737/1‑150 (MQ=255)
                                                                                                         cgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAcgcg                                        >  2:1085760/1‑151 (MQ=255)
                                                                                                         cgtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAAcgcg                                        >  3:573376/1‑151 (MQ=255)
                                                                                                          gtGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGa                                       <  2:165318/151‑1 (MQ=255)
                                                                                                            gCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACg                                     >  2:1459592/1‑151 (MQ=255)
                                                                                                             ccTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGc                                    >  1:1654150/1‑151 (MQ=255)
                                                                                                                gTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCAt                                 >  1:1391998/1‑151 (MQ=255)
                                                                                                                    cAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgc                             <  1:867744/151‑1 (MQ=255)
                                                                                                                    cAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCGCGATGCTGAACGCGACGCCATCcgc                             <  1:1336507/151‑1 (MQ=255)
                                                                                                                            tttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTc                     >  1:315055/1‑151 (MQ=255)
                                                                                                                                            cccGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCcgcg         <  2:414973/147‑1 (MQ=255)
                                                                                                                                            cccGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGt        <  2:1627157/148‑1 (MQ=255)
                                                                                                                                            cccGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCg      <  3:170154/150‑1 (MQ=255)
                                                                                                                                              cGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGccc   >  2:1166241/1‑151 (MQ=255)
                                                                                                                                               gTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCt  <  1:157286/151‑1 (MQ=255)
                                                                                                                                               gTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCt  <  1:1197028/151‑1 (MQ=255)
                                                                                                                                               gTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCt  <  1:1166739/151‑1 (MQ=255)
                                                                                                                                               gTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCt  <  2:1504955/151‑1 (MQ=255)
                                                                                                                                               gTCCACCCATATTGACCTGTGTTTTGTCAGAACAGGTGTCGGGCTCCACGGTTAACACGCCACCATTTGCCAGCAGGCTATTGTTCGCCGCAACCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCt  >  2:152608/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCT  >  NC_000913/2780183‑2780476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: