Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,828,902 A→G L94L (TTA→TTG)  srlM → sorbitol=responsive srl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,9020AG97.9% 525.7 / ‑1.6 143L94L (TTA→TTG) srlMsorbitol=responsive srl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (63/77);  total (65/78)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCGGGCGCTTTAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTACAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACGCCCATG  >  NC_000913/2828757‑2829049
                                                                                                                                                 |                                                                                                                                                   
gCGGGCGCTTTAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGc                                                                                                                                                 <  2:1007201/150‑1 (MQ=255)
      gcTTTAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGAtgt                                                                                                                                          >  1:865749/1‑151 (MQ=255)
      gcTTTAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGAtgt                                                                                                                                          <  3:222920/151‑1 (MQ=255)
          tAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGAt                                                                                                                                       <  2:1141885/150‑1 (MQ=255)
             aCCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCttt                                                                                                                                   <  1:1390184/151‑1 (MQ=255)
               cgcgGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTcc                                                                                                                                 <  3:413697/151‑1 (MQ=255)
               cgcgGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTcc                                                                                                                                 >  2:1292730/1‑151 (MQ=255)
                gcgGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTccc                                                                                                                                >  1:969234/1‑151 (MQ=255)
                 cgGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTcccc                                                                                                                               <  1:1229160/151‑1 (MQ=255)
                  gggTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTcccc                                                                                                                               >  2:1319433/1‑150 (MQ=255)
                   ggTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCAt                                                                                                                             <  1:708318/151‑1 (MQ=255)
                    gacGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACATTGTTTATGAAAGGAATGAGCGTCTTCGCCCTAACGCAAAAAATTCCCGCAATGACCGGGATGCATGCGGGAGATTTACAGCCCGATGTGATCTTTccc                                                                                                                                >  4:311864/3‑147 (MQ=255)
                    gTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATg                                                                                                                            <  2:1345107/151‑1 (MQ=255)
                      cGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGAt                                                                                                                          >  2:231579/1‑151 (MQ=255)
                      cGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGAt                                                                                                                          <  2:989569/151‑1 (MQ=255)
                      cGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGAt                                                                                                                          >  3:594900/1‑151 (MQ=255)
                             gCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTAt                                                                                                                   <  2:1391274/151‑1 (MQ=255)
                             gCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTAt                                                                                                                   >  4:157641/1‑151 (MQ=255)
                             gCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTAt                                                                                                                   >  4:571844/1‑151 (MQ=255)
                               cATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATca                                                                                                                 <  2:843524/151‑1 (MQ=255)
                                  cgcgCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATcaca                                                                                                               <  2:180501/150‑1 (MQ=255)
                                  cgcgCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAg                                                                                                              <  1:1613001/151‑1 (MQ=255)
                                   gcgcTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATcac                                                                                                                >  1:1108373/1‑148 (MQ=255)
                                   gcgcTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATcac                                                                                                                >  1:1113863/1‑148 (MQ=255)
                                    cgcTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGaa                                                                                                            <  3:152866/151‑1 (MQ=255)
                                     gcTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCTCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGaa                                                                                                            <  2:1644458/150‑1 (MQ=255)
                                     gcTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAAt                                                                                                           <  3:483192/151‑1 (MQ=255)
                                          aCGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGctct                                                                                                      >  2:246277/1‑151 (MQ=255)
                                              tCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATc                                                                                                   >  3:591492/1‑150 (MQ=255)
                                                 gcagcGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTg                                                                                               <  4:136321/151‑1 (MQ=255)
                                                  cagcGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTgg                                                                                              <  2:1422308/151‑1 (MQ=255)
                                                  cagcGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTgg                                                                                              <  2:53318/151‑1 (MQ=255)
                                                    gcgcATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGgcg                                                                                            >  3:255868/1‑151 (MQ=255)
                                                     cgcATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGgcgc                                                                                           <  1:175817/151‑1 (MQ=255)
                                                      gcATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGgcgc                                                                                           <  1:911370/150‑1 (MQ=255)
                                                        aTCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCtt                                                                                         >  1:425583/1‑150 (MQ=255)
                                                          cgtcgACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTaa                                                                                       <  3:157662/150‑1 (MQ=255)
                                                          cgtcgACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTaa                                                                                       <  2:872801/150‑1 (MQ=255)
                                                          cgtcgACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTaa                                                                                       <  1:721115/150‑1 (MQ=255)
                                                           gtcgACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAAc                                                                                     >  1:1017870/1‑151 (MQ=255)
                                                             cgACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTg                                                                                   >  2:802281/1‑151 (MQ=255)
                                                              gACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTg                                                                                   >  2:564232/1‑150 (MQ=255)
                                                                cacCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGaa                                                                                 >  2:413584/1‑150 (MQ=255)
                                                                  ccTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAAc                                                                               <  1:563354/150‑1 (MQ=255)
                                                                   cTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGt                                                                             >  2:204942/1‑151 (MQ=255)
                                                                      gTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTgg                                                                           >  3:44931/1‑150 (MQ=255)
                                                                       tttATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGAt                                                                         >  2:385811/1‑151 (MQ=255)
                                                                        ttATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGAt                                                                         <  2:1076748/150‑1 (MQ=255)
                                                                        ttATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGAt                                                                         >  2:645292/1‑150 (MQ=255)
                                                                        ttATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGAt                                                                         >  1:737988/1‑150 (MQ=255)
                                                                         tATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATa                                                                        <  2:429653/150‑1 (MQ=255)
                                                                         tATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATa                                                                        <  2:1682202/150‑1 (MQ=255)
                                                                          aTGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATaa                                                                       <  2:527479/150‑1 (MQ=255)
                                                                            gAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAAttt                                                                    <  4:370089/151‑1 (MQ=255)
                                                                            gAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAAtt                                                                     <  1:1187797/150‑1 (MQ=255)
                                                                               tggACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGt                                                                 >  1:782546/2‑151 (MQ=255)
                                                                                 gACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTtg                                                               <  1:535424/151‑1 (MQ=255)
                                                                                  aCTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTtgt                                                              >  2:1207924/1‑151 (MQ=255)
                                                                                    tGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTtgtg                                                             >  1:695414/1‑150 (MQ=255)
                                                                                     gACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTtgtg                                                             <  1:1789/149‑1 (MQ=255)
                                                                                     gACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGa                                                            <  2:1421034/150‑1 (MQ=255)
                                                                                       ccGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATg                                                         <  1:470736/151‑1 (MQ=255)
                                                                                       ccGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATg                                                         <  1:1501152/151‑1 (MQ=255)
                                                                                        cGTCTTCTCCCGACCTGAAAAAATTCCCGCAATTACCGTTATGCATGCGGGTGATTTGCAGCCCGATGTCATCTTTCCCCATGATCCACTATCACAGAATGCTCTAGCATTGTCGCTTAAACTGAAACGTTGATAGTTTCGTTGTGAATg                                                         <  1:1368579/150‑1 (MQ=255)
                                                                                           cTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTAc                                                     >  2:97356/1‑151 (MQ=255)
                                                                                              cgatcGACAGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCGGCCCGATGTGATCTTTCCACATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTActgg                                                  >  1:484701/5‑149 (MQ=255)
                                                                                                  cGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTActtgcttg                                              >  1:268988/1‑151 (MQ=255)
                                                                                                  cGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTActtgcttg                                              <  4:39243/151‑1 (MQ=255)
                                                                                                    aCCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCg                                            >  3:457023/1‑151 (MQ=255)
                                                                                                         aaaaaaTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGtt                                       >  1:1559051/1‑151 (MQ=255)
                                                                                                         aaaaaaTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGtt                                       >  2:868789/1‑151 (MQ=255)
                                                                                                         aaaaaaTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGtt                                       <  3:500185/151‑1 (MQ=255)
                                                                                                          aaaaaTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGtt                                       >  1:1351323/1‑150 (MQ=255)
                                                                                                             aaTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCa                                   <  4:412412/151‑1 (MQ=255)
                                                                                                              aTTCCCGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCAt                                  >  2:1033260/1‑151 (MQ=255)
                                                                                                                 cccGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCAttt                                <  3:437066/150‑1 (MQ=255)
                                                                                                                  ccGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTg                              <  1:898089/151‑1 (MQ=255)
                                                                                                                   cGCAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTg                              >  2:1562165/1‑150 (MQ=255)
                                                                                                                     cAATTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGaa                            >  1:373055/1‑150 (MQ=255)
                                                                                                                      aaTTACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAAc                          <  2:1016509/151‑1 (MQ=255)
                                                                                                                         tACCGGTATGCATGCGGGTGATTTGCAGCCTGATTTGATCTTTCCCCATGATCCACTATTACAGATTGCTCTATTATTTCCGCGTAAACTGAATCGTGTATAATTTCGTTGTGAATGTTACTTGCTTGCGATGTTATCATTTTGAAAcat                        <  1:214007/150‑3 (MQ=255)
                                                                                                                         tACCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCt                        >  2:1187304/1‑150 (MQ=255)
                                                                                                                          aCCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTaa                      <  1:1717180/151‑1 (MQ=255)
                                                                                                                          aCCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTaa                      <  4:500968/151‑1 (MQ=255)
                                                                                                                          aCCGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTa                       <  2:816256/150‑1 (MQ=255)
                                                                                                                           ccGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTaa                      >  2:1714683/1‑150 (MQ=255)
                                                                                                                            cGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAAt                    <  1:1456216/151‑1 (MQ=255)
                                                                                                                            cGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAAt                    <  3:226578/151‑1 (MQ=255)
                                                                                                                            cGGTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAAt                    >  1:1421829/1‑151 (MQ=255)
                                                                                                                             ggTATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTaa                      >  4:152418/1‑148 (MQ=255)
                                                                                                                               tATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATc                   >  2:1128474/1‑149 (MQ=255)
                                                                                                                               tATGCATGCGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATc                   >  2:1360880/1‑149 (MQ=255)
                                                                                                                                  gcatgcGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGt               <  3:103762/150‑1 (MQ=255)
                                                                                                                                  gcatgcGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGt               >  1:1584051/1‑150 (MQ=255)
                                                                                                                                  gcatgcGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTa              <  1:688815/151‑1 (MQ=255)
                                                                                                                                    atgcGGGTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAAt            >  2:1229850/1‑151 (MQ=255)
                                                                                                                                        gggTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACg        <  1:340190/151‑1 (MQ=255)
                                                                                                                                        gggTGATTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACg        >  2:1561122/1‑151 (MQ=255)
                                                                                                                                             aTTTGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACGCCCAt   >  2:1175362/1‑151 (MQ=255)
                                                                                                                                              tttGCAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACGCCCATg  >  2:115742/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGGGCGCTTTAAACCGCGGGTCGTGGTCGCCATCGCGCTGGACGATCAGCAGCGCATCGTCGACACCTTGTTTATGAAAGGACTGACCGTCTTCGCCCGACCGCAAAAAATTCCCGCAATTACCGGTATGCATGCGGGTGATTTACAGCCCGATGTGATCTTTCCCCATGATCCACTATCACAGAATGCTCTATCATTGGCGCTTAAACTGAAACGTGGATAATTTCGTTGTGAATGTTACTTGCTTGCGAAGTTATCATTTTGAAACCTAAATCAGGTAATCACGCCCATG  >  NC_000913/2828757‑2829049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑