Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,975,171 G→A P282S (CCC→TCC)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,975,1710GA99.3% 516.8 / NA 137P282S (CCC→TCC) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (61/75);  minor base C (1/0);  total (62/75)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGG  >  NC_000913/2975023‑2975317
                                                                                                                                                    |                                                                                                                                                  
aCCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACa                                                                                                                                                  <  2:1419399/151‑1 (MQ=255)
 cccAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACa                                                                                                                                                  >  2:1388674/1‑150 (MQ=255)
   cAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATg                                                                                                                                                >  1:1467979/1‑150 (MQ=255)
    aGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATgcg                                                                                                                                              <  4:194674/151‑1 (MQ=255)
    aGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCCGTGTAGTTCATCATTGCGGACATgcg                                                                                                                                              >  1:392503/1‑151 (MQ=255)
     gcgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATgcgg                                                                                                                                             >  2:1663981/1‑151 (MQ=255)
     gcgcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATgcgg                                                                                                                                             <  2:852050/151‑1 (MQ=255)
       gcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATgcggc                                                                                                                                            <  2:410485/150‑1 (MQ=255)
       gcACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCCCCGTAATAGCACTGGGCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACACgcggc                                                                                                                                            >  1:1207297/1‑150 (MQ=255)
        cacgagataaaggggCTCCGGCAGTTGCGAGAGTTTCCCTTAAAGGAGAAACTGGTGGGGAGTGAAGAGGTTTTTGATTCCAGCCGCCGTAATAGCACTGGACAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATgcggcg                                                                                                                                           <  1:1388303/136‑1 (MQ=255)
         aCCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGa                                                                                                                                          >  1:829823/1‑150 (MQ=255)
          ccTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACg                                                                                                                                        >  4:118018/1‑151 (MQ=255)
           cTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACgg                                                                                                                                       >  1:1652702/1‑151 (MQ=255)
            tGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACggg                                                                                                                                      >  2:773675/1‑151 (MQ=255)
            tGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCCACggg                                                                                                                                      >  3:90723/1‑151 (MQ=255)
             gAGTAAGTTGCTCCGGCAGATGCAAGAGTTTGCCTTTATAGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGAc                                                                                                                                         <  1:171169/147‑1 (MQ=255)
                 aaGTTGCTCCGGCAGATGCCAGAGTTTGTCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGAt                                                                                                                                  <  2:28244/150‑1 (MQ=255)
                    ttGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATg                                                                                                                                 <  2:1287128/148‑1 (MQ=255)
                           ggCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGGGAAGCTGGCTTTGGTTTCAGCCGCCGTAATAGGACAGGCCAGCCCTTTTACCCCGGCAGGGGAATTCATCCTTCCGGCCATGCCGGGACCGGCGATGGCCCCaaaa                                                                                                                        >  4:283475/1‑150 (MQ=255)
                             cAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATc                                                                                                                     <  4:305857/151‑1 (MQ=255)
                                       agTTTGACTTTATGGAGAATCGGGCGGGAAGTGTAGTTTATTAGAATATCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCAGCAGTGTAGTTCATCATTTCGGACATGCGGCGACGGTCGATGGCCCCAAAAATCACTg                                                                                                                 <  1:1643837/145‑1 (MQ=255)
                                         tttGCCTTTATCGAGAAACGGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACt                                                                                                          <  2:1670915/150‑1 (MQ=255)
                                           tGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGa                                                                                                        <  4:49892/150‑1 (MQ=255)
                                           tGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGAt                                                                                                       >  1:732219/1‑151 (MQ=255)
                                           tGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGAt                                                                                                       >  3:319948/1‑151 (MQ=255)
                                                ttATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCt                                                                                                   <  1:1346100/150‑1 (MQ=255)
                                                  aTCGAGAAACTGGCGGGAAGTGGAGATGGTTTTGATTTCAGCCGCCGTAATAGCGCTGGTCCGCCCTTTTACCCCGGCAGTGTAGGTCATCATTGCGGACATGCGGCGACGGGCGATGGACCCAAAAATCACTGCCGCACTGATGCCTg                                                                                                  >  4:26154/1‑149 (MQ=255)
                                                  aTCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCa                                                                                                <  4:55110/151‑1 (MQ=255)
                                                  aTCGAGAAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTg                                                                                                  >  2:847883/1‑149 (MQ=255)
                                                   tCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCa                                                                                                <  3:178940/150‑1 (MQ=255)
                                                     gagaAACTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTg                                                                                             >  2:795182/1‑151 (MQ=255)
                                                      agaACCGGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTg                                                                                             <  1:21699/150‑1 (MQ=255)
                                                      agaAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTgg                                                                                            <  2:59955/151‑1 (MQ=255)
                                                      agaAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTg                                                                                             <  2:1436451/150‑1 (MQ=255)
                                                         aaCTGGCGGGAGGTGAAGAGGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgca                                                                                         <  1:117492/151‑1 (MQ=255)
                                                         aaCTGGCGGGAAGTGAAGATGGGTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTTCGGACATGCGGCGACGGGCTATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgc                                                                                          >  1:1046693/1‑150 (MQ=255)
                                                            tGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCCCTGATGCCCGCATTGGgcagc                                                                                       >  1:730229/1‑150 (MQ=255)
                                                             ggCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGgcagc                                                                                       <  2:41370/149‑1 (MQ=255)
                                                             ggCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCAt                                                                                     <  2:602316/151‑1 (MQ=255)
                                                              gCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCAtt                                                                                    >  3:224845/1‑151 (MQ=255)
                                                                gggAAGTGAAGATGGTTTTGTTTGCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTaa                                                                                  <  2:674101/151‑1 (MQ=255)
                                                                   aaGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGc                                                                                <  2:1078944/150‑1 (MQ=255)
                                                                    aGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCg                                                                              >  3:113128/1‑151 (MQ=255)
                                                                    aGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCg                                                                              >  2:166662/1‑151 (MQ=255)
                                                                     gTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGa                                                                             <  2:337019/151‑1 (MQ=255)
                                                                        aaGATGGTTTTGTTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATg                                                                           <  1:489012/150‑1 (MQ=255)
                                                                         aGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCg                                                                         <  4:186636/151‑1 (MQ=255)
                                                                             ggTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTc                                                                      <  1:71009/150‑1 (MQ=255)
                                                                             ggTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCg                                                                     >  3:532354/1‑151 (MQ=255)
                                                                               ttttGATTTCACCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCg                                                                     <  1:1676099/149‑1 (MQ=255)
                                                                                tttGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGGTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCt                                                                  >  1:1451270/1‑151 (MQ=255)
                                                                                 ttGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCt                                                                  <  1:43336/150‑1 (MQ=255)
                                                                                  tGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt                                                                 <  2:792968/150‑1 (MQ=255)
                                                                                  tGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc                                                                >  4:435209/1‑151 (MQ=255)
                                                                                   gATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc                                                                >  2:268378/1‑150 (MQ=255)
                                                                                    aTTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCaa                                                              <  3:316046/151‑1 (MQ=255)
                                                                                       tCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAacac                                                           >  2:696954/1‑151 (MQ=255)
                                                                                        cAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACt                                                          <  3:594974/151‑1 (MQ=255)
                                                                                          gccgccGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGt                                                        >  1:861525/1‑151 (MQ=255)
                                                                                           ccgccgTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGt                                                        >  2:1029386/1‑150 (MQ=255)
                                                                                           ccgccgTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTa                                                       <  2:1041895/151‑1 (MQ=255)
                                                                                                 tAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTc                                                 >  1:1030088/1‑151 (MQ=255)
                                                                                                  aaTAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTc                                                 <  2:1278297/150‑1 (MQ=255)
                                                                                                  aaTAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCa                                                <  2:1207437/151‑1 (MQ=255)
                                                                                                   aTAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCa                                                >  1:1366433/1‑150 (MQ=255)
                                                                                                    tAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCa                                                >  1:177804/1‑149 (MQ=255)
                                                                                                     aGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAgg                                             <  3:485642/151‑1 (MQ=255)
                                                                                                     aGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAg                                              <  2:472813/150‑1 (MQ=255)
                                                                                                      gCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAgg                                             >  2:1711558/1‑150 (MQ=255)
                                                                                                       cACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGAt                                           <  1:1540906/151‑1 (MQ=255)
                                                                                                       cACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGAt                                           >  2:644062/1‑151 (MQ=255)
                                                                                                         cTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGc                                         >  1:983238/1‑151 (MQ=255)
                                                                                                           ggTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGt                                       <  2:1425932/151‑1 (MQ=255)
                                                                                                              cAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCca                                    <  2:1519889/151‑1 (MQ=255)
                                                                                                               aGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCca                                    >  2:383624/1‑150 (MQ=255)
                                                                                                                     tttACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCaacaaac                              >  2:1330115/1‑150 (MQ=255)
                                                                                                                     tttACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCaacaaa                               <  2:387778/149‑1 (MQ=255)
                                                                                                                     tttACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCaacaaa                               <  1:1125853/149‑1 (MQ=255)
                                                                                                                      ttACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCaacaaaca                             <  2:1537893/150‑1 (MQ=255)
                                                                                                                      ttACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTCTATTTCCCAAGGATGCGTCCaacaaaca                             <  1:422038/150‑1 (MQ=255)
                                                                                                                       tACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAg                            <  2:227105/150‑1 (MQ=255)
                                                                                                                       tACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAg                            <  2:50854/150‑1 (MQ=255)
                                                                                                                       tACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGc                           >  1:515795/1‑151 (MQ=255)
                                                                                                                       tACCCCGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGc                           >  2:870821/1‑151 (MQ=255)
                                                                                                                            cGGCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGtttt                      <  1:950559/151‑1 (MQ=255)
                                                                                                                              gCAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCg                    <  1:1430711/151‑1 (MQ=255)
                                                                                                                               cAGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGt                   <  2:463645/151‑1 (MQ=255)
                                                                                                                                aGTGTAGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGt                   >  1:1555898/1‑150 (MQ=255)
                                                                                                                                     aGTTCATCATTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCaa             <  2:307135/151‑1 (MQ=255)
                                                                                                                                        tcatcaTTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAAttt          >  1:1189699/1‑151 (MQ=255)
                                                                                                                                         catcatTGCGGACATGCGGCGTCGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGTAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTg         <  4:430662/151‑1 (MQ=255)
                                                                                                                                         catcatTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAAttt          <  2:380207/150‑1 (MQ=255)
                                                                                                                                         catcatTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTg         >  4:536462/1‑151 (MQ=255)
                                                                                                                                         catcatTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGATCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGCTTTCGTAAGCAAttt          >  1:1060989/1‑150 (MQ=255)
                                                                                                                                          atcatTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGc        >  1:1080220/1‑151 (MQ=255)
                                                                                                                                            catTGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCg       >  1:98688/1‑150 (MQ=255)
                                                                                                                                               tGCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGAt     >  1:175995/1‑149 (MQ=255)
                                                                                                                                                gCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAgg  >  1:1413645/1‑151 (MQ=255)
                                                                                                                                                gCGGACATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAgg  >  3:357278/1‑151 (MQ=255)
                                                                                                                                                gCGGACATACGGCCACGCGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCCGCATTGGGCAGCATTAAGCCGCTGCGTTGGCCTTCAACACTGTATTTTTCCAGGTTGCGTCCAACAATCAGCGTTTACGTAAGCAATTTGCGATa    >  1:1725222/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAAGTGAAGATGGTTTTGATTTCAGCCGCCGTAATAGCACTGGTCAGCCCTTTTACCCCGGCAGTGTAGTTCATCATTGCGGGCATGCGGCGACGGGCGATGGCCCCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTCAACACTGTATTTTTCAAGGATGCGTCCAACAAACAGCGTTTTCGTAAGCAATTTGCGATAGG  >  NC_000913/2975023‑2975317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: