Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,086,618 A→G intergenic (+216/‑88) yqgC → / → metK uncharacterized protein/S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,086,6180AG99.4% 621.0 / NA 165intergenic (+216/‑88)yqgC/metKuncharacterized protein/S‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (77/87);  total (77/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 166 total aligned reads displayed.

TGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGA  >  NC_000913/3086473‑3086767
                                                                                                                                                  |                                                                                                                                                     
tGTCAGGAAAAATTTCCAGCCCCGTTTTTAACAGAATGAGACACGATTCAAAAACAAGCGGAAATAGGGCGTAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCAAGCGTTGTTGACCTg                                                                                                                                                  >  1:1655454/1‑151 (MQ=255)
tGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCt                                                                                                                                                   <  2:1670147/150‑1 (MQ=255)
tGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCt                                                                                                                                                   <  2:533986/150‑1 (MQ=255)
 gTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTg                                                                                                                                                  <  2:1388491/150‑1 (MQ=255)
 gTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTg                                                                                                                                                  <  2:703286/150‑1 (MQ=255)
  tCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGc                                                                                                                                                 >  2:765110/1‑150 (MQ=255)
   cAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGc                                                                                                                                                 >  2:842586/1‑149 (MQ=255)
          aaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTg                                                                                                                                        <  2:937664/151‑1 (MQ=255)
           aTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTgg                                                                                                                                       <  2:1112696/151‑1 (MQ=255)
             ttCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGt                                                                                                                                      >  2:63034/1‑150 (MQ=255)
             ttCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGt                                                                                                                                      >  1:302627/1‑150 (MQ=255)
                cAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAgg                                                                                                                                   >  2:183012/1‑150 (MQ=255)
                cAGCCACGTTTTTAACAGAAAGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTa                                                                                                                                     <  1:1193675/148‑1 (MQ=255)
                   ccACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATcc                                                                                                                               <  1:1475511/151‑1 (MQ=255)
                   ccACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATcc                                                                                                                               <  3:418801/151‑1 (MQ=255)
                          tttAACAGAATGAGACACGATTCAAAAAAAAAGGGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTAcc                                                                                                                         >  1:1380901/1‑151 (MQ=255)
                          tttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTAcc                                                                                                                         >  3:185737/1‑150 (MQ=255)
                           ttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcac                                                                                                                       >  4:529358/1‑151 (MQ=255)
                             aaCAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAg                                                                                                                     <  2:1172759/151‑1 (MQ=255)
                                 gAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaaaa                                                                                                                 <  1:886305/151‑1 (MQ=255)
                                     gagaCACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATcc                                                                                                              >  2:1688681/1‑150 (MQ=255)
                                       gaCACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCcaca                                                                                                           <  2:1250734/151‑1 (MQ=255)
                                           cGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACacaac                                                                                                       >  2:466694/1‑151 (MQ=255)
                                             aTTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAg                                                                                                     <  1:233142/151‑1 (MQ=255)
                                             aTTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAg                                                                                                     <  1:1098758/151‑1 (MQ=255)
                                               tCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGtt                                                                                                   >  1:1039711/1‑151 (MQ=255)
                                                cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                  >  1:1357532/1‑151 (MQ=255)
                                                cAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGttt                                                                                                  >  1:39944/1‑151 (MQ=255)
                                                  aaaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                                 <  1:568828/151‑1 (MQ=255)
                                                     aaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGc                                                                                              >  2:1102383/1‑151 (MQ=255)
                                                       aaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTa                                                                                            <  4:462429/151‑1 (MQ=255)
                                                       aaaGAGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCt                                                                                             >  4:328404/1‑150 (MQ=255)
                                                           tGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAAcc                                                                                         >  4:340731/1‑150 (MQ=255)
                                                              aaaTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGGCTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAAt                                                                                     <  1:947834/151‑1 (MQ=255)
                                                               aaTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAAtt                                                                                    <  2:1257906/151‑1 (MQ=255)
                                                                aTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAATACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtc                                                                                   >  1:1692226/1‑151 (MQ=255)
                                                                aTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtc                                                                                   <  1:1234371/151‑1 (MQ=255)
                                                                 tAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtct                                                                                  >  2:327910/1‑151 (MQ=255)
                                                                  aGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATtct                                                                                  <  2:1553617/150‑1 (MQ=255)
                                                                       gaagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAg                                                                            <  4:319894/151‑1 (MQ=255)
                                                                        aagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAgg                                                                           <  2:1438976/151‑1 (MQ=255)
                                                                         agaaTTGACCTAAAATATCCATCCATATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTctct                                                                                <  2:1412824/145‑1 (MQ=255)
                                                                         agaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAgg                                                                           >  1:17021/1‑150 (MQ=255)
                                                                         agaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGt                                                                          >  2:56316/1‑151 (MQ=255)
                                                                         agaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGt                                                                          <  2:438565/151‑1 (MQ=255)
                                                                         agaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGt                                                                          <  2:516356/151‑1 (MQ=255)
                                                                          gaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTg                                                                         <  2:479072/151‑1 (MQ=255)
                                                                          gaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTg                                                                         <  1:860030/151‑1 (MQ=255)
                                                                               gACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGatat                                                                     <  4:44444/150‑1 (MQ=255)
                                                                                aCCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTa                                                                   <  1:1625211/151‑1 (MQ=255)
                                                                                aCCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTAAGCTAACCAAATTCTCTTTAGGGAatat                                                                     >  1:1073573/1‑149 (MQ=255)
                                                                                 ccTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                                  <  1:902293/151‑1 (MQ=255)
                                                                                 ccTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTa                                                                   >  3:549246/1‑150 (MQ=255)
                                                                                  cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                                  >  1:504672/1‑150 (MQ=255)
                                                                                   tAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTACAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                                >  2:488913/1‑151 (MQ=255)
                                                                                    aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                                >  4:237669/1‑150 (MQ=255)
                                                                                    aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAat                                                                >  2:290342/1‑150 (MQ=255)
                                                                                     aaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAatat                                                              >  2:987477/1‑151 (MQ=255)
                                                                                     aaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAatat                                                              >  2:42010/1‑151 (MQ=255)
                                                                                      aaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATg                                                             <  2:354268/151‑1 (MQ=255)
                                                                                         aGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGc                                                           <  2:546425/150‑1 (MQ=255)
                                                                                         aGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCa                                                          <  3:551702/151‑1 (MQ=255)
                                                                                         aGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCa                                                          <  3:52997/151‑1 (MQ=255)
                                                                                          gCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaa                                                         <  2:877122/151‑1 (MQ=255)
                                                                                           ccATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                        <  1:301402/151‑1 (MQ=255)
                                                                                            cATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCaaa                                                        >  3:126428/1‑150 (MQ=255)
                                                                                             aTCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAac                                                      >  2:1536413/1‑151 (MQ=255)
                                                                                             aTCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAac                                                      >  1:1383489/1‑151 (MQ=255)
                                                                                               ccAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAacac                                                    <  1:864436/151‑1 (MQ=255)
                                                                                                  gATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtt                                                 <  4:376723/151‑1 (MQ=255)
                                                                                                  gATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtt                                                 <  4:459594/151‑1 (MQ=255)
                                                                                                   aTGTTAATCCATCCATACCGATTAGCACTCAGACTGCCAGTGTATTAGAACTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATAATAAATATGGCAAAACACCttt                                                <  1:1621747/151‑1 (MQ=255)
                                                                                                   aTGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCttt                                                >  1:456667/1‑151 (MQ=255)
                                                                                                   aTGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCttt                                                >  2:1590425/1‑151 (MQ=255)
                                                                                                       tAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTa                                             <  2:960513/150‑1 (MQ=255)
                                                                                                        aaTCCATCAATACCGAGTAACACTCAGACTGCTAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAACATCCACACAACAATTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACg                                           >  1:1475565/1‑151 (MQ=255)
                                                                                                         atccatccATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGt                                          <  1:1607688/151‑1 (MQ=255)
                                                                                                          tccatccaTACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTc                                         >  2:1679614/1‑151 (MQ=255)
                                                                                                           ccatccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTc                                         <  4:373799/150‑1 (MQ=255)
                                                                                                            catccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCg                                       <  2:208719/151‑1 (MQ=255)
                                                                                                               ccatACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGt                                    <  2:1400291/151‑1 (MQ=255)
                                                                                                                 atACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTcc                                  <  2:1477662/151‑1 (MQ=255)
                                                                                                                       aTTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTctct                            <  1:703940/151‑1 (MQ=255)
                                                                                                                       aTTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTctct                            >  1:314272/1‑151 (MQ=255)
                                                                                                                       aTTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTctct                            >  2:1515313/1‑151 (MQ=255)
                                                                                                                           acacTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGaa                         <  1:1318736/150‑1 (MQ=255)
                                                                                                                           acacTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGa                          >  3:57390/1‑149 (MQ=255)
                                                                                                                           acacTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAg                        <  2:1022927/151‑1 (MQ=255)
                                                                                                                                cAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCAt                   <  2:1655618/151‑1 (MQ=255)
                                                                                                                                 aGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATc                  >  2:745/1‑151 (MQ=255)
                                                                                                                                   aCTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCt                >  2:13165/1‑151 (MQ=255)
                                                                                                                                    cTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTg               >  4:142330/1‑151 (MQ=255)
                                                                                                                                     tGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTg               >  1:584995/1‑150 (MQ=255)
                                                                                                                                        cAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaa           <  4:153497/151‑1 (MQ=255)
                                                                                                                                          gtgtTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaaa         <  3:434282/151‑1 (MQ=255)
                                                                                                                                          gtgtTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaa          >  2:1643799/1‑150 (MQ=255)
                                                                                                                                            gtTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAAt        <  1:1338213/150‑1 (MQ=255)
                                                                                                                                             tttttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTg      >  1:1012879/1‑151 (MQ=255)
                                                                                                                                              ttttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTg      <  2:983032/150‑1 (MQ=255)
                                                                                                                                              ttttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTg      <  2:594955/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGTCAGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGA  >  NC_000913/3086473‑3086767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: