BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,151,501	T→C	T162A (ACG→GCG)	exbB ←	membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,151,501	0	T	C	100.0%	539.6 / NA	146	T162A (ACG→GCG)	exbB	membrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (78/68); total (78/68)

Only 100 of 147 total aligned reads displayed.

CACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGG  >  NC_000913/3151354‑3151646

cACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGctt                                                                                                                                                 <  2:442599/150‑1 (MQ=255)

cACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTg                                                                                                                                                <  4:230064/151‑1 (MQ=255)

 aCCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTgc                                                                                                                                               >  2:55041/1‑151 (MQ=255)

  cccAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTgcg                                                                                                                                              <  1:447240/151‑1 (MQ=255)

  cccAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTgcg                                                                                                                                              >  1:241350/1‑151 (MQ=255)

   ccAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTgcgc                                                                                                                                             <  3:464856/151‑1 (MQ=255)

     aGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGa                                                                                                                                           <  2:590502/151‑1 (MQ=255)

        aTCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATAc                                                                                                                                        <  2:956555/151‑1 (MQ=255)

           gCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCa                                                                                                                                      >  3:184771/1‑150 (MQ=255)

                aaaGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATaaa                                                                                                                                 <  1:995196/150‑1 (MQ=255)

                aaaGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATaa                                                                                                                                  >  1:334142/1‑149 (MQ=255)

                 aaGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGc                                                                                                                               <  1:632066/151‑1 (MQ=255)

                  aGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCt                                                                                                                              >  2:51480/1‑151 (MQ=255)

                  aGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGGGGTCTGCGCTTGCGCGATACCAATAAAGCt                                                                                                                              >  2:285071/1‑151 (MQ=255)

                    ccgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGt                                                                                                                            >  2:270940/1‑151 (MQ=255)

                    ccgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGt                                                                                                                            >  1:48949/1‑151 (MQ=255)

                    ccgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTGGCGCGATAACAGTAAAGCTGt                                                                                                                            >  1:1067282/1‑151 (MQ=255)

                     cgccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGtt                                                                                                                           >  2:614602/1‑151 (MQ=255)

                      gccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTc                                                                                                                          <  1:1043610/151‑1 (MQ=255)

                       ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTc                                                                                                                          >  2:1724884/1‑150 (MQ=255)

                       ccAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTc                                                                                                                          >  1:652196/1‑150 (MQ=255)

                        cAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCat                                                                                                                        >  1:1179765/1‑151 (MQ=255)

                        cAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCat                                                                                                                        >  2:1366614/1‑151 (MQ=255)

                         aaTCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCatg                                                                                                                       >  2:526939/1‑151 (MQ=255)

                          aTCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCatg                                                                                                                       >  2:564172/1‑150 (MQ=255)

                             tGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGc                                                                                                                   >  3:413394/1‑151 (MQ=255)

                              ggCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGcc                                                                                                                  >  1:310759/1‑151 (MQ=255)

                                   gCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAg                                                                                                              <  2:1056913/150‑1 (MQ=255)

                                      aaTACGTTATAGATAACGAGCGCAGGAATCGCTGCCACGAGGCCGATTGCAGTTGCTAACAGAACTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGAc                                                                                                            <  1:517050/149‑1 (MQ=255)

                                      aaTACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAATAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCg                                                                                                          >  3:486622/1‑151 (MQ=255)

                                       aTACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGt                                                                                                         >  1:157326/1‑151 (MQ=255)

                                         aCGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCACTAAAGCTGTTCATGATGCCCCAGACCGTAc                                                                                                       >  1:1440902/1‑151 (MQ=255)

                                         aCGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTa                                                                                                        <  2:342970/150‑1 (MQ=255)

                                          cGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTAcc                                                                                                      >  1:101547/1‑151 (MQ=255)

                                             tataGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCaa                                                                                                    >  1:1618952/1‑150 (MQ=255)

                                             tataGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCaa                                                                                                    >  3:583112/1‑150 (MQ=255)

                                             tataGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCaa                                                                                                    >  3:80764/1‑150 (MQ=255)

                                              ataGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAAc                                                                                                  >  2:884797/1‑151 (MQ=255)

                                                aGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAg                                                                                                <  2:1734212/151‑1 (MQ=255)

                                                 gATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGa                                                                                               <  2:190019/151‑1 (MQ=255)

                                                  aTAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGAc                                                                                              <  1:1715813/151‑1 (MQ=255)

                                                       gACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacg                                                                                         >  2:1003106/1‑151 (MQ=255)

                                                        aCCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacga                                                                                        <  2:1130129/151‑1 (MQ=255)

                                                        aCCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacg                                                                                         >  2:449311/1‑150 (MQ=255)

                                                         ccGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacga                                                                                        >  3:132851/1‑150 (MQ=255)

                                                          cGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacgaac                                                                                      <  2:303455/151‑1 (MQ=255)

                                                           gCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacgaacg                                                                                     >  1:127756/1‑151 (MQ=255)

                                                           gCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacgaacg                                                                                     <  2:952527/151‑1 (MQ=255)

                                                           gCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCaacgaacg                                                                                     <  2:1051612/151‑1 (MQ=255)

                                                             aGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACgg                                                                                    >  2:143827/1‑150 (MQ=255)

                                                             aGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACgg                                                                                    >  4:476695/1‑150 (MQ=255)

                                                             aGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGga                                                                                   <  1:1002814/151‑1 (MQ=255)

                                                               gAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGgaga                                                                                 <  4:311769/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  4:184751/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  2:449856/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  3:304511/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  1:1390458/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  1:1682416/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  1:1692349/151‑1 (MQ=255)

                                                                   cGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             <  1:331360/151‑1 (MQ=255)

                                                                    gCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATcg                                                                            >  1:436754/1‑151 (MQ=255)

                                                                    gCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATc                                                                             >  1:927814/1‑150 (MQ=255)

                                                                     cTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATcgc                                                                           >  1:896528/1‑151 (MQ=255)

                                                                       gCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATcgcg                                                                          <  2:339394/150‑1 (MQ=255)

                                                                       gCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATcgc                                                                           <  2:768435/149‑1 (MQ=255)

                                                                       gCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATcgc                                                                           <  3:490788/149‑1 (MQ=255)

                                                                            gAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATg                                                                    >  3:343530/1‑151 (MQ=255)

                                                                            gAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATg                                                                    <  1:875264/151‑1 (MQ=255)

                                                                              ggCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGt                                                                  <  2:1570651/151‑1 (MQ=255)

                                                                                    ttGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGcc                                                              >  1:662331/1‑149 (MQ=255)

                                                                                    ttGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGcc                                                              >  2:1477803/1‑149 (MQ=255)

                                                                                      gCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGa                                                           <  2:1225704/150‑1 (MQ=255)

                                                                                       ccGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATa                                                         <  2:202095/151‑1 (MQ=255)

                                                                                        cGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGAt                                                          >  1:545009/1‑149 (MQ=255)

                                                                                              tAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGtt                                                   >  1:606330/1‑150 (MQ=255)

                                                                                                aCAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTAcc                                                >  4:341581/1‑151 (MQ=255)

                                                                                                aCAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTAcc                                                <  2:497256/151‑1 (MQ=255)

                                                                                                 cAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACcg                                               <  2:718470/151‑1 (MQ=255)

                                                                                                     gCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGc                                           >  3:289219/1‑151 (MQ=255)

                                                                                                      cTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGcc                                          >  1:963507/1‑151 (MQ=255)

                                                                                                          tGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAtt                                      >  2:1172672/1‑151 (MQ=255)

                                                                                                           gCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAttt                                     <  1:547688/151‑1 (MQ=255)

                                                                                                           gCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCAttt                                     <  2:1441768/151‑1 (MQ=255)

                                                                                                            cGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTg                                    >  2:667925/1‑151 (MQ=255)

                                                                                                              aTACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgac                                  <  2:842132/151‑1 (MQ=255)

                                                                                                              aTACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgac                                  >  2:1426935/1‑151 (MQ=255)

                                                                                                               tACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgac                                  >  2:447847/1‑150 (MQ=255)

                                                                                                                aCCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgacg                                 <  2:74965/150‑1 (MQ=255)

                                                                                                                aCCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTgacg                                 <  2:1224280/150‑1 (MQ=255)

                                                                                                                  ccGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGAcc                              >  2:1421813/1‑151 (MQ=255)

                                                                                                                  ccGGCGCAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGAcc                              >  4:219433/1‑151 (MQ=255)

                                                                                                                    ggCGCAACAACTGCCAGGTGAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGGTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGATATCGCGCCGAAGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGAcc                              >  1:35056/1‑149 (MQ=255)

                                                                                                                      cgcAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACt                          <  4:554556/151‑1 (MQ=255)

                                                                                                                      cgcAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACt                          <  2:1318565/151‑1 (MQ=255)

                                                                                                                        cAACGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTgc                        <  1:1634617/151‑1 (MQ=255)

                                                                                                                           cGACTGCCAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTgcggc                     <  2:1497007/151‑1 (MQ=255)

                                                                                                                                 ccAGGTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCg               >  2:329327/1‑151 (MQ=255)

                                                                                                                                     gTTAGTGGTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGc           >  1:426662/1‑151 (MQ=255)

                                                                                                                                           ggTCTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAg      <  1:969450/150‑1 (MQ=255)

                                                                                                                                              cTGCGCTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACgg  >  4:183463/1‑151 (MQ=255)

CACCCAGCATCGCTTTAAAGCCGCCAATCTGGCGTGCAAATACGTTATAGATAACGACCGCAGGAATCGCTGCCACGAGGCCGATTGCCGTTGCTAACAGAGCTTCTGCGATACCCGGCGCAACGACTGCCAGGTTAGTGGTCTGCGTTTGCGCGATACCAATAAAGCTGTTCATGATGCCCCAGACCGTACCAAACAGACCAACGAACGGAGAAATCGCGCCGATGGTTGCCAGATAGCCGTTACCGCGGCCCATTTGACGACCCACTGCGGCGACCCGACGCTCCAGACGG  >  NC_000913/3151354‑3151646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`