Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,272,271	Δ1 bp	coding (481/885 nt)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,272,268	0	C	.	100.0%	162.7 / NA	35	coding (484/885 nt)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/22);  total (13/22)

GCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCA  >  NC_000913/3272123‑3272413                                                                                                                                                  |                                                                                                                                                  gCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTcccggt                                                                                                                                              >  2:646592/1‑148 (MQ=255)   cAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTAt                                                                                                                                           <  1:423053/151‑1 (MQ=255)              gAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGa                                                                                                                                 >  1:430786/1‑150 (MQ=255)               aaTTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTTCCTGCACCGATTT‑CCCGGTATGCACCACGGAAc                                                                                                                               <  1:549121/151‑1 (MQ=255)                          aaaCCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCt                                                                                                                    <  1:120481/151‑1 (MQ=255)                                        ttAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt                                                                                                       >  2:105874/1‑150 (MQ=255)                                         tAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGTCGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATa                                                                                                      <  1:152594/150‑1 (MQ=255)                                         tAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATa                                                                                                      <  2:486757/150‑1 (MQ=255)                                         tAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑ACCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt                                                                                                       >  2:646130/1‑149 (MQ=255)                                           aCGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTa                                                                                                   <  1:178344/151‑1 (MQ=255)                                            cGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTAt                                                                                                  <  1:403826/151‑1 (MQ=255)                                                cGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGt                                                                                              >  2:246480/1‑151 (MQ=255)                                                     tAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa                                                                                          <  1:127057/150‑1 (MQ=255)                                                      aGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaa                                                                                         <  1:627734/150‑1 (MQ=255)                                                         tGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATa                                                                                      <  1:125453/150‑1 (MQ=255)                                                                gTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTc                                                                              >  2:324071/1‑151 (MQ=255)                                                                  tAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTcg                                                                             <  1:115698/150‑1 (MQ=255)                                                                   aaCGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTcgc                                                                            <  2:334556/150‑1 (MQ=255)                                                                              cATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCa                                                                <  2:326867/151‑1 (MQ=255)                                                                                tCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt                                                              >  1:124902/1‑151 (MQ=255)                                                                                  gcgGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGa                                                            <  2:41751/151‑1 (MQ=255)                                                                                     gCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAg                                                         <  1:35171/151‑1 (MQ=255)                                                                                      cAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGc                                                        <  1:410741/151‑1 (MQ=255)                                                                                          atTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTAc                                                    >  1:395535/1‑151 (MQ=255)                                                                                                         gACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCg                                     >  1:289758/1‑151 (MQ=255)                                                                                                              gATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTc                                 <  1:586140/150‑1 (MQ=255)                                                                                                                  tGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc                            >  1:314013/1‑151 (MQ=255)                                                                                                                        tttGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTc                       >  1:166504/1‑150 (MQ=255)                                                                                                                               aCGTTACCTGCACCGATTT‑CCCGTTATGCACCACGGAACCCTCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCCCCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGc               <  1:141925/151‑1 (MQ=255)                                                                                                                                 gTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCAt             >  2:9973/1‑151 (MQ=255)                                                                                                                                  ttACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAACTCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGc               >  2:565929/1‑148 (MQ=255)                                                                                                                                     ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCa          <  1:548441/150‑1 (MQ=255)                                                                                                                                     ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAg         <  1:122051/151‑1 (MQ=255)                                                                                                                                       tGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGc        >  2:225773/1‑150 (MQ=255)                                                                                                                                        gCACCGATTT‑CCCGGTATGCACCACGGACCCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCCCCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCat      <  2:38748/151‑1 (MQ=255)                                                                                                                                            cGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCa  <  1:336230/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCA  >  NC_000913/3272123‑3272413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑