| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 121,578 | (T)7→6 | intergenic (‑27/‑514) | aroP ← / → pdhR | aromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 121,572 | 0 | T | . | 82.6% | 66.7 / 6.9 | 23 | intergenic (‑21/‑520) | aroP/pdhR | aromatic amino acid transporter/pyruvate dehydrogenase complex repressor; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base . (6/13); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCG > NC_000913/121423‑121704 | ggACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTTACCTTCCCTCATGAAACCTCGTGCGGGGgttgtt > 2:476418/1‑151 (MQ=255) ggAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACg < 2:198028/151‑1 (MQ=255) ggAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGGGGGTG‑TTTTTTGATCTAc > 2:35022/1‑150 (MQ=255) ggAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAAACTCGTGCGGGGGGTG‑TTTTTTGATCTa > 2:540576/1‑149 (MQ=255) cAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGGTTTTAAGGCCGCGCTTTAGCTGCCCGCCGGGCTGTTTACCTTCCATCATGAAAACTCGTGCGGGGGGTGTTTTTTT‑ATTTTCGCCGTGATGCgt > 1:64095/1‑150 (MQ=255) ggAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCgtgtg < 1:66467/151‑1 (MQ=255) cccGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAAGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCCTGAAAACCCGTGCGGGGGGTG‑TTTTTTGATCTACGCCGTGATGCGTGTGTAAGttt > 1:480477/1‑151 (MQ=255) gTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCaa < 2:141407/151‑1 (MQ=255) cccTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAAtt < 1:495963/151‑1 (MQ=255) cTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGGTGTTGACCTTCCATCATGAAACCTCGTGCGGGGGGTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTcc > 2:138965/1‑151 (MQ=255) aTCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTTACCTTCCATCATGAAACCCCGTGCGGGGGGTGTTTTTTTG‑TCTACGCAGTGATGCGTGTGTAAGTTTGCCATTTCGt > 2:325723/1‑151 (MQ=255) cgcCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAAACTCGTGCGGGGGTTGTTTTTTT‑TTCTACGCAGTGATGCGTGTGTAAGTTTGCAATTTCGTttgt > 1:548411/1‑151 (MQ=255) ccACCCAGCGCGATAAGCTGAATATGGCGGTTTTTTAAGGCGCGCCTTAGCTGCTCGCCGGGGTGTTGACCTTCCATCATGAAAAATCGTGCGGGGGGTG‑GTTTTTGATCTTAGCAGTGATGCGTGTGTAAGTTTTCAAATCCGTttg > 1:22850/1‑148 (MQ=255) aCCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTa < 2:125542/151‑1 (MQ=255) cgATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAAttt < 2:372543/150‑1 (MQ=255) gCTGAATATGGCGGGTTTTAAGGGCGCGCTTTAGCTGGCCGCCGGGGTGTTGACCCTCCCTTATGAAACCTCGGGGGGGGGGTGTTTTTTT‑ATCTACGCCGGGGTTCGGGGGGAAGTTTGCAATTCCGTTTGTTGGATTAAAtttttt > 1:199549/1‑148 (MQ=255) ttAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAg < 1:637253/149‑1 (MQ=255) gctCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTcc < 1:641106/149‑1 (MQ=255) tCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTc > 2:226422/1‑150 (MQ=255) cGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTc < 2:15794/149‑1 (MQ=255) tCCATCATGAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTa < 1:476415/150‑1 (MQ=255) gAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAg < 2:242947/150‑1 (MQ=255) gAAACCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGc < 1:5561/151‑1 (MQ=255) aaaCCTCGTGCGGTGGTTG‑TTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCg < 2:306654/151‑1 (MQ=255) | GGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCATGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCG > NC_000913/121423‑121704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |