Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,054	(C)7→8	coding (1279/2526 nt)	ecpC ←	ECP production outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,047	1	.	C	100.0%	107.0 / NA	31	A429G (GCG→GGG)	ecpC	ECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (18/13);  total (18/13)

ATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGC  >  NC_000913/307899‑308184                                                                                                                                                      |                                                                                                                                          aTCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑cc                                                                                                                                         <  1:509215/151‑1 (MQ=255)  tCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑ccc                                                                                                                                        >  2:514713/1‑151 (MQ=255)        cGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑cccccccca                                                                                                                                  <  1:557667/151‑3 (MQ=255)         gTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑cccccccca                                                                                                                                  >  1:443671/1‑148 (MQ=255)                 tgattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGc                                                                                                                          >  2:268974/1‑151 (MQ=255)                  gattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGc                                                                                                                          <  2:91860/150‑1 (MQ=255)                          aTGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACgg                                                                                                                 >  2:182986/1‑151 (MQ=255)                          aTGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACgg                                                                                                                 >  2:176313/1‑151 (MQ=255)                            gCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGt                                                                                                               <  1:411668/151‑1 (MQ=255)                                           aTTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                                                                >  1:216051/1‑151 (MQ=255)                                           aTTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                                                                >  1:668711/1‑151 (MQ=255)                                            ttAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                                                                >  2:657994/1‑150 (MQ=255)                                            ttAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTg                                                                                                >  2:521718/1‑150 (MQ=255)                                              aaCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTCCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAtt                                                                                             >  2:573582/1‑151 (MQ=255)                                                 ccACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATc                                                                                          <  2:614366/151‑1 (MQ=255)                                                    caGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCGGACGGGTTTCACCCACCGCCTGATTATCGt                                                                                        >  1:514870/1‑150 (MQ=255)                                                          aCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCata                                                                                 <  1:327899/151‑1 (MQ=255)                                                             aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATcc                                                                              <  2:47495/151‑1 (MQ=255)                                                                    cccGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTg                                                                         <  1:130692/149‑1 (MQ=255)                                                                      cGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccgc                                                                     >  1:425815/1‑151 (MQ=255)                                                                                gcgcTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGc                                                           <  2:52654/151‑1 (MQ=255)                                                                                     gATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTa                                                        <  1:290868/149‑1 (MQ=255)                                                                                     gATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACt                                                      <  1:447247/151‑1 (MQ=255)                                                                                      aTGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTc                                                     <  1:356585/151‑1 (MQ=255)                                                                                                 gctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGa                                          <  2:425820/151‑1 (MQ=255)                                                                                                         aTGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAgg                                  >  1:167046/1‑151 (MQ=255)                                                                                                          tGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGc                                 >  1:478645/1‑151 (MQ=255)                                                                                                               tGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTg                            >  1:508831/1‑151 (MQ=255)                                                                                                               tGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTg                            >  2:442136/1‑151 (MQ=255)                                                                                                                     tGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGcc                      >  2:210378/1‑151 (MQ=255)                                                                                                                       gCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCa                     >  2:405690/1‑150 (MQ=255)                                                                                                                             atatTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAActctct              >  1:174515/1‑151 (MQ=255)                                                                                                                              tatTTTGCAGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCtt             >  2:6973/1‑151 (MQ=255)                                                                                                                                      aGGTTAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGt     <  1:194977/151‑1 (MQ=255)                                                                                                                                          tAACGTTGATCGCCCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTcgc  <  1:442131/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                          ATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGC  >  NC_000913/307899‑308184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑