Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,5240GA44.1% 34.2 / 17.0 34intergenic (‑542/+61)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (3/16);  new base A (15/0);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 4.48e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTC  >  NC_000913/987384‑987671
                                                                                                                                             |                                                                                                                                                   
aTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTTGAAAAAAAATTGTTTAATTTAAAAACAAATAAAtt                                                                                                                                              >  1:522963/1‑148 (MQ=255)
   gtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTCTTTTATTAACAAAAAAATAAATTGAc                                                                                                                                           >  2:399805/1‑148 (MQ=255)
     gCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGaa                                                                                                                                       <  2:529335/150‑1 (MQ=255)
            gCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaa                                                                                                                               <  1:602283/151‑1 (MQ=255)
              tAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTTCTTTTCTTTACAAACAAATAACTTTACAGAAAAAATaaaa                                                                                                                              >  2:33689/1‑150 (MQ=255)
                 gAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTCTTTACTTTAAAAAAAAATAAATTTACAGAAAAGAaaaaaaaa                                                                                                                           >  2:477165/1‑150 (MQ=255)
                  aaaaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAaga                                                                                                                         <  1:250576/151‑1 (MQ=255)
                            tCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGGAATAGATTGTTTGTCTTAACAAACAAATAACTTTTAAGAAAAGAAAAAAAAAAAGCCCACggg                                                                                                                >  2:662406/1‑150 (MQ=255)
                             ccGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg                                                                                                              <  1:616234/151‑1 (MQ=255)
                              cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATGTTTGACTTTAAAAACAAAAAAAATTACAGAAAAGAAAAAAAAAAAACCAGCGGgcg                                                                                                              >  1:518407/1‑150 (MQ=255)
                                   tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTTTAAAAATAATTTTTTTCTTTACAAAAAAAAAAATTTAACGAAAAAAAAAAAAAAAAAACCGCGGGCGCtcg                                                                                                          >  2:509982/1‑147 (MQ=255)
                                        aTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGa                                                                                                   <  1:109852/151‑1 (MQ=255)
                                         tGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAGAATGCTTGACTTAACAAAAAAAAAAATTTACAGAAAAAATAAAAAAAAAAACAGCGGGGGCTGGGTCtt                                                                                                     >  2:553914/1‑148 (MQ=255)
                                         tGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGCAAAAAAAATGCTTTTCTTTAAAAACAAATAAAATGACAGAAAAGAAAAAAAAAAAAACAGCGGGCGCTGGGTCTTgt                                                                                                   >  1:159547/1‑149 (MQ=255)
                                          gCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGaa                                                                                                  <  1:207297/150‑1 (MQ=255)
                                            ggATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAATTGTTTTTCTTTAAAAACAAAAAAATTTACAGAAAAGATAAAAAAAAAAGCAGCGGGGGCTGGCTCCTGaa                                                                                                  >  2:438718/1‑148 (MQ=255)
                                                     tttGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAAtt                                                                                       <  2:323439/150‑1 (MQ=255)
                                                       tGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGTAAAAAAAATTTTTGTCTTTAAAAACAAGAAAAATGAAAGAAAAAATAAAAAAAAAAGCAGCGGGGGCCGGGCCTTTTAGAAGCTaaaat                                                                                       >  2:225438/1‑146 (MQ=255)
                                                         cgcgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGGGGAAAAAAAATATTTTATTTAACAAAAAAATAAATTGGTCGGAAAAAAAAAAAAAAAAACAACGGGGGCTGGGGCTTGAAGAAGACAAAATAGaa                                                                                   >  1:666821/1‑150 (MQ=255)
                                                           cgTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCt                                                                                <  1:460010/151‑1 (MQ=255)
                                                              aaaTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAAAATTCTTTAGTTTACAAAAAAATAAATTGACAGAAAAAAAAAAAAAAAAGACAGCGGGCGCCGGGGCCTGAAGAAGcccaaataaaagatg                                                                               >  2:204941/1‑135 (MQ=255)
                                                                aTGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGt                                                                           <  2:468040/151‑1 (MQ=255)
                                                                               atTCCGTAACAGGATGATCGTTATGGCGGGGGTGGAAAAAGATTGCTTTACTTAAAAAAAAAAAAAATTTACAGAAAAGAAAAAAAAAAAAACAGCGGGCGCTGGCTCTTGTAGACGCTAAAATAAAAAATGGCGTTACGCGGAGGACGTg                                                            >  2:367094/1‑151 (MQ=255)
                                                                                     tAACAGGATGATCGTTATGGCGGGGGTGTAAATAGATTTCATGACTTTACAAAAAAAAAAAATGTCAGAAAAAAAAAAAAAAAAACCCGCCGGCGCTGGCTCCTTTAGAACCCCAAATAGAAACCGGCGTTAAGCggcggaaatggg                                                          >  2:222142/1‑137 (MQ=255)
                                                                                        cAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGaa                                                   <  2:31593/151‑1 (MQ=255)
                                                                                        cAGGATGATCGTTATGGCGGGGGTTTAAATAGATATCTTTTTTTAACAAACAGATAAATTTGCAGAAAAAAAAAAAAAAAAAACAGAGGGGGCTGGCTCTTGAAGAAACTAAAATATAAAGTGCCGGTAAGCCGAGAACGTggg                                                          >  1:391867/1‑144 (MQ=255)
                                                                                                  gTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCacg                                         <  1:553909/151‑1 (MQ=255)
                                                                                                                               tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc              <  2:111495/150‑1 (MQ=255)
                                                                                                                               tGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGc              >  1:457773/1‑150 (MQ=255)
                                                                                                                                    tAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATc          <  1:63695/149‑1 (MQ=255)
                                                                                                                                      acaaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGAc      <  2:598436/151‑1 (MQ=255)
                                                                                                                                       caaacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACg     >  1:24758/1‑151 (MQ=255)
                                                                                                                                         aacaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGtt   <  1:52298/151‑1 (MQ=255)
                                                                                                                                          acaGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTc  <  2:71294/151‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCTCTTGAAGACGCTAAATTAGAAGCTGGCGTTACGCGGAGTACGTGGGAACGGAATCACATCACGTACGTTTTGCACGCCAGTTACGTAAGCAATCAGACGTTC  >  NC_000913/987384‑987671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: