| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 46.7% | 50.6 / 23.9 | 45 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (6/18); new base G (21/0); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATT > NC_000913/1478178‑1478472 | tGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGAGA‑GGAAAAGGGGGGAgg > 2:472666/1‑151 (MQ=255) gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGt < 1:474434/151‑1 (MQ=255) gATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTa < 2:489605/151‑1 (MQ=255) aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGAGG‑GAAAAGGGGGGGGGGGTACa > 1:446042/1‑148 (MQ=255) ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAAGGGGGGGGGGTACATTTCGTAtt > 1:314941/1‑148 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGA‑GGAAAGGGGGGGGGGTGACATTTAGTAtt > 2:271437/1‑147 (MQ=255) cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCa < 1:664147/151‑1 (MQ=255) aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCCGAGA‑GGAAAGTGGGGGAGGTTACATTACGTATTTGTAACTGGGGGGGGATCGTCAtt > 2:129313/1‑148 (MQ=255) aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGG‑GAAAAAGGGGGGAGGGTACATTTCGTATTCGTACCTGGTGGGGGCTCGTCATTTGtt > 1:387377/1‑151 (MQ=255) tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GGAAAGGGGGGGGGGGTAAATTTAGTAATCGTAACTTGTGGGGGATAGTCCtttttttt > 1:403295/1‑151 (MQ=255) tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGG‑GAAAAAGGGGGGAGGGTTGATTATCTCTTTCGAACTGGTGGGGGATCGGCATTTGgttt > 1:228791/1‑151 (MQ=255) aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTc < 2:20427/150‑1 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGAGG‑GAAAAAGGGGGGGGGTTTCATTATGTATTTGTACCTGGGGGGGGATCGTGATTTGtttttttat > 1:442868/1‑141 (MQ=255) tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGGGA‑GAAAAAGGGGGGAGGTTAAATTTCGTATTTGTACCTGGGGGGGGATCGTCCTTTTTTTTTTTATGGGGGTTTTAGGGcc > 1:145951/1‑151 (MQ=255) ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGA‑GAAAAAGGGGTGGGGGTACATTACGGATTTGGACCTGGTGGGGGCTCGTCATTTTTTTTTCTATGGGGGTTTTAGGTccc > 2:582714/1‑150 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAt < 2:256493/150‑1 (MQ=255) tCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtga < 1:489873/150‑1 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGATACATTTACTATTTGTACCTTGGGGGGGATTGGCAATTTTTTTTTTATTGTGGttttcaggccaccccgg > 1:290805/1‑131 (MQ=255) gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGG‑GGAAACGGGGGGGGGGTAAAATACTTATTTGTAACTCGGGGGGGGACGTCATTTTTTTTTTTATGGTGATTTTATGTCCCCCCGGGAt > 1:351056/1‑146 (MQ=255) cgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACCGGGA‑GAAAAAGGGGGGGGGGTACAATACGTATTTTTACCTCGTGGGGGATCGTCCTTTTTTTTTTTATGGTGATTTTAGGTCCACCCTTGAtggcggt > 1:156823/1‑149 (MQ=255) gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACCGTGG‑GAAAAGGGGGGGAGGGTATATTAAGTATTTGTACCTTGTGGGGGGTCGTCCTTTTTTTTTTTATTGTGGTTTTTGGGCCACGCTGGGtgacggt > 1:93195/1‑148 (MQ=255) tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgttga < 1:514569/150‑1 (MQ=255) ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGAGGGTACATTACGTATTCGTACCTGGTGGGGGATCGTCATTTGTTTTTTAATGGTGATTTTAGGTCCACGCTGGGTGACGTTGTCGttttt > 2:152380/1‑151 (MQ=255) aGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGCGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg < 1:116380/151‑1 (MQ=255) aGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTTCCGGGGAGGAAAGGGGGGGGAGGAT‑CATTACGTTATCGTACCCGGGGGGGGAACGGCATTTTTTTTTTAATGGTGAGTTTTGGACCCCGCAGGGTGGCGTTTACGgttttt > 2:593020/1‑148 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGGGA‑GGAAAGGGGGGGGGGGTACATTACGTATTTGTAACTGGTGGGGGGACGTCATTTTTTTTTTTATGGGGGTTTTAGGGCCACGCTGGGTGCAGTTGACGtttttttt > 1:523326/1‑145 (MQ=255) cGCTGATAAACCGTTTTCTGCTATGGCGGTTATTGGGGA‑GAAAAAGGGGGGAGGTTTTATTACGCATTTGGGACTCGGGGGGGAACGTCATTTTTTTTTCCATTGTGATTTCATGGCCACGCCTGGGGACGTTGTCGGTTTTTtcg > 1:507225/1‑146 (MQ=255) cGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGAt < 2:397147/151‑1 (MQ=255) gCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGG‑GAAAAAGGGGGGGGGGTACATTAAGTATTTGTTACTGGTGGGGGATCGGCATTTGTTTTTTAATGGGGGTTTTAGGGCCACGCTGGGTGACGTTGACGgttttt > 2:68235/1‑142 (MQ=255) gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATaa < 1:348975/149‑1 (MQ=255) aaaCCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAAGGGGGGGGGTTACATTTCGTATTCGTACCCGGGGGGGCATCGTCATTTTTTTTTTAATGGTGGTTTTAGGTCCACGCTGGGTGACGTTGACGTTTTTTGCGCTGATAAGtttt > 2:359643/1‑151 (MQ=255) aaaCCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtttt < 2:292400/151‑1 (MQ=255) ccGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGGTACATTTCGTATTTGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATGGGGATTTTAGGGCCACGCTGGGTGACGTTGGCGTTTTTTTTGCTGGTAAGtttttt > 1:238935/1‑148 (MQ=255) gCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTg < 2:257584/148‑1 (MQ=255) aTGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAg < 2:479249/149‑1 (MQ=255) gCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAatat < 1:83457/151‑1 (MQ=255) tACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc > 1:173371/1‑151 (MQ=255) ccGGTGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGAGATTTCAGGTCCATGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGc > 1:384769/1‑151 (MQ=255) ggAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt > 2:665826/1‑151 (MQ=255) ga‑gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata < 2:523329/151‑1 (MQ=255) ga‑gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat > 1:624098/1‑150 (MQ=255) aaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg > 2:115683/1‑151 (MQ=255) aaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg < 1:280073/151‑1 (MQ=255) aGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTa < 1:161072/151‑1 (MQ=255) ggTGAGGTTACATTACGTATTCGTACATGGTGAGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTCCGCTTATATCTGTACAta < 1:648900/151‑2 (MQ=255) | TGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATT > NC_000913/1478178‑1478472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |