Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,463	G→A	P11L (CCG→CTG)	satP ←	succinate‑acetate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,463	0	G	A	100.0%	211.1 / NA	55	P11L (CCG→CTG)	satP	succinate‑acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/21);  total (34/21)

TTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGA  >  NC_000913/10317‑10609                                                                                                                                                   |                                                                                                                                                   ttttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTg                                                                                                                                                 <  2:306685/150‑1 (MQ=255)   ttGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCg                                                                                                                                              >  2:620339/1‑151 (MQ=255)    tGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCgg                                                                                                                                             >  1:124080/1‑151 (MQ=255)        cTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGAtt                                                                                                                                          >  1:286599/1‑150 (MQ=255)               agACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACt                                                                                                                                  <  2:670841/151‑1 (MQ=255)                   cAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAgt                                                                                                                              >  2:657482/1‑151 (MQ=255)                     gCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCgg                                                                                                                                             >  1:605918/1‑134 (MQ=255)                      cAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCgg                                                                                                                                             <  2:605924/133‑1 (MQ=255)                       aaaaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTg                                                                                                                          <  2:288269/151‑1 (MQ=255)                         aaaTTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGcc                                                                                                                        <  2:373131/151‑1 (MQ=255)                               gcgcGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAAt                                                                                                                  <  1:397855/151‑1 (MQ=255)                                    aTGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTc                                                                                                             >  2:388690/1‑151 (MQ=255)                                      gccgccGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCa                                                                                                            >  2:560473/1‑150 (MQ=255)                                        cgccgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCaaa                                                                                                          >  2:540703/1‑150 (MQ=255)                                           cgTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                                      <  1:540698/151‑1 (MQ=255)                                            gTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatc                                                                                                      >  2:496458/1‑150 (MQ=255)                                             tAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcat                                                                                                    >  2:330084/1‑151 (MQ=255)                                                aaaaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAatcatcat                                                                                                 <  2:421883/151‑1 (MQ=255)                                                  aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCg                                                                                               >  2:640969/1‑151 (MQ=255)                                                  aaTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCg                                                                                               <  2:511719/151‑1 (MQ=255)                                                   aTGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCg                                                                                               >  2:352680/1‑150 (MQ=255)                                                        cATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCgaatga                                                                                          >  1:607317/1‑150 (MQ=255)                                                          tGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGaatgaatg                                                                                       >  2:476904/1‑151 (MQ=255)                                                              aaGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTg                                                                                    >  2:27187/1‑150 (MQ=255)                                                                  ataataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaat                                                                                >  1:172671/1‑150 (MQ=255)                                                                    aataCCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAaataa                                                                              <  2:581837/150‑1 (MQ=255)                                                                          gTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCt                                                                       <  2:172673/151‑1 (MQ=255)                                                                            ccAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTg                                                                      <  1:396372/150‑1 (MQ=255)                                                                                agCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATaa                                                                 <  2:214269/151‑1 (MQ=255)                                                                                  cGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACt                                                               >  1:585499/1‑151 (MQ=255)                                                                                      aTAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAg                                                           <  2:233658/151‑1 (MQ=255)                                                                                        aaCCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAG‑CCATCAGGCCCAACAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAGCt                                                               <  1:367775/144‑1 (MQ=255)                                                                                          cccACGTTGTGTAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtt                                                       >  2:403904/1‑151 (MQ=255)                                                                                           ccACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGttt                                                      >  1:507263/1‑151 (MQ=255)                                                                                            cACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGtttt                                                     >  1:377743/1‑151 (MQ=255)                                                                                                ttGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAgg                                                 >  1:568664/1‑151 (MQ=255)                                                                                                 tgtgCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAggg                                                >  2:373462/1‑151 (MQ=255)                                                                                                        gTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCa                                         <  1:363174/151‑1 (MQ=255)                                                                                                         ttAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                                                           >  1:488726/1‑116 (MQ=255)                                                                                                         ttAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAAttt                                                                           <  2:488728/116‑1 (MQ=255)                                                                                                         ttAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCat                                        >  2:657514/1‑151 (MQ=255)                                                                                                               aGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGAGCCGGATTAGCCAACTTTGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATaataat                                  >  1:222449/1‑151 (MQ=255)                                                                                                                 aaTGGTGGTCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATc                                 >  1:568091/1‑150 (MQ=255)                                                                                                                       ggtCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGt                          >  2:123801/1‑151 (MQ=255)                                                                                                                       ggtCATGCCGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGt                          >  1:374323/1‑151 (MQ=255)                                                                                                                               cGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAgt                  >  2:480418/1‑151 (MQ=255)                                                                                                                               cGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAgt                  >  1:669765/1‑151 (MQ=255)                                                                                                                               cGAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAg                   >  1:149346/1‑150 (MQ=255)                                                                                                                                gAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAgtg                 >  2:10271/1‑151 (MQ=255)                                                                                                                                gAAGCCCATCAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAgtg                 <  1:631405/151‑1 (MQ=255)                                                                                                                                         cAGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGAt         <  2:609056/150‑1 (MQ=255)                                                                                                                                          aGGCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCt       >  1:644081/1‑151 (MQ=255)                                                                                                                                            gCCCAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCttt     <  2:248433/151‑1 (MQ=255)                                                                                                                                              ccAGCAGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTg   <  1:259227/151‑1 (MQ=255)                                                                                                                                              ccAGCAGTGCCGGATTAGCCAACTTAGCGTGGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCtttt    >  1:120521/1‑150 (MQ=255)                                                                                                                                               cagcagTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGa  <  2:374327/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TTTTGTACTCCAGCAGACCAGCAAAAATTTGCGCGATGCCGCCGTAGAAAATGCCCATGGCAAGAATAATACCGTCCAGAGCGAAATAACCCACGTTGTGCAGGTTAAGCAGAATGGTGGTCATGCCGAAGCCCATCAGGCCCAGCGGTGCCGGATTAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGA  >  NC_000913/10317‑10609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑