Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	264,465	C→A	E90* (GAG→TAG)	ykfG ←	CP4‑6 prophage; RadC‑like JAB domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	264,465	0	C	A	100.0%	157.2 / NA	41	E90* (GAG→TAG)	ykfG	CP4‑6 prophage; RadC‑like JAB domain protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/20);  total (21/20)

GGACACGGATATCCACCAGCTGAAGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTCCCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACGTACGGCGTG  >  NC_000913/264318‑264612                                                                                                                                                    |                                                                                                                                                    ggACACGGATATCCACCAGCTGAAGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGTGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCg                                                                                                                                                  <  1:216812/151‑1 (MQ=255)                  aGCTGAAGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGc                                                                                                                                 >  1:230894/1‑151 (MQ=255)                      gAAGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGt                                                                                                                              <  2:227714/150‑1 (MQ=255)                        aGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTaa                                                                                                                           <  2:650624/151‑1 (MQ=255)                         gCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAAt                                                                                                                          <  2:535772/151‑1 (MQ=255)                                   cAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTg                                                                                                                >  2:537062/1‑151 (MQ=255)                                           gCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCg                                                                                                         <  1:481187/150‑1 (MQ=255)                                                    ttttGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGc                                                                                               >  1:176332/1‑151 (MQ=255)                                                       tGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCaa                                                                                             <  1:581295/150‑1 (MQ=255)                                                        gTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATc                                                                                           <  1:62954/151‑1 (MQ=255)                                                            gCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACt                                                                                       <  2:142035/151‑1 (MQ=255)                                                              cTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTg                                                                                      >  1:607802/1‑150 (MQ=255)                                                                 gCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTc                                                                                  >  2:461642/1‑151 (MQ=255)                                                                   tAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTg                                                                                >  1:332232/1‑151 (MQ=255)                                                                    aGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTg                                                                                <  1:585649/150‑1 (MQ=255)                                                                          cgtcTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTcc                                                                         <  1:316117/151‑1 (MQ=255)                                                                              tcGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTcc                                                                         >  2:522486/1‑147 (MQ=255)                                                                                gCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATac                                                                   >  1:438336/1‑151 (MQ=255)                                                                                 ccGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATaca                                                                  <  1:322442/151‑1 (MQ=255)                                                                                    gAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATacaac                                                                >  1:157911/1‑150 (MQ=255)                                                                                        gATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCg                                                           <  1:474582/151‑1 (MQ=255)                                                                                          tGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCgg                                                          >  1:665056/1‑150 (MQ=255)                                                                                             ttATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAAct                                                      >  2:530077/1‑151 (MQ=255)                                                                                              tATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAAct                                                      <  1:546228/150‑1 (MQ=255)                                                                                                         aTCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCa                                           <  1:12191/150‑1 (MQ=255)                                                                                                           cACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAgc                                        >  1:611447/1‑151 (MQ=255)                                                                                                           cACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAgc                                        >  1:299369/1‑151 (MQ=255)                                                                                                           cACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAgc                                        <  1:195795/151‑1 (MQ=255)                                                                                                              cgccgccgcGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGcgcg                                     <  2:153109/151‑1 (MQ=255)                                                                                                              cgccgccgcGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGcgcg                                     <  1:573100/151‑1 (MQ=255)                                                                                                                 cgccgcGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGcc                                  >  2:423155/1‑151 (MQ=255)                                                                                                                  gccgcGTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGcc                                  >  1:71098/1‑150 (MQ=255)                                                                                                                    cgcgTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGACata                               <  1:22490/151‑1 (MQ=255)                                                                                                                     gcgTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCatat                              >  1:624247/1‑151 (MQ=255)                                                                                                                     gcgTTGAAGTGCAGAGCACGATTGACAACCTACCGGGGCTGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGACCatat                              >  1:665557/1‑151 (MQ=255)                                                                                                                       gTTGAAGTGCAGAGCACGTTTGACCACCTACCGGGGATCCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGc                            >  2:623584/1‑151 (MQ=255)                                                                                                                             gTGCAGAGCACGTTTGACCACCTACCGGGGATCCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGc                      <  1:623574/151‑1 (MQ=255)                                                                                                                                        gTTTGACCACCTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACg           >  2:141114/1‑151 (MQ=255)                                                                                                                                                accTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACGTACGGCGt   <  2:141127/151‑1 (MQ=255)                                                                                                                                                accTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACGTACGGCGt   >  2:608090/1‑151 (MQ=255)                                                                                                                                                 ccTACCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACGTACGGCGTg  >  1:111498/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGACACGGATATCCACCAGCTGAAGCACCTGAACCAGTCGCTGCGTGAGGGTTTTGTCGGCCTGGCTAGGTGTCGTCTCGCCGGAAGGATGGTTATGCGCGAGTATCACCGCCGCCGCGTTGAAGTGCAGAGCACGTTTGACCACCTCCCGGGGATGCACCTCGGTGCGGTTAATCGTGCCGGTGAAGAGCGTTTCATGGGCAATCAACTGATTCTGGTTGTCCAGATACAACACCCGGAACTCTTCCCGCTCAAGCGCGGCCATATGCAGTCGCAGCCATTCACGTACGGCGTG  >  NC_000913/264318‑264612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑