Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,272,752	C→T	P130L (CCC→CTC)	yejA →	microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,272,752	0	C	T	100.0%	163.7 / NA	42	P130L (CCC→CTC)	yejA	microcin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/22);  total (20/22)

AGCAGCTGTACGACACGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCCCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCAAAGAAG  >  NC_000913/2272605‑2272898                                                                                                                                                    |                                                                                                                                                   agcagcTGTACGACACGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCAt                                                                                                                                                 <  1:86967/151‑1 (MQ=255)     gcTGTACGACACGCTATGTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACt                                                                                                                                             <  1:329122/151‑1 (MQ=255)         tACGACACGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGccc                                                                                                                                         >  1:514414/1‑151 (MQ=255)             acacGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCcgcg                                                                                                                                      >  2:246864/1‑150 (MQ=255)               acGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATg                                                                                                                                   <  2:206030/151‑1 (MQ=255)                    aTTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTaga                                                                                                                               <  2:575435/150‑1 (MQ=255)                    aTTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTaga                                                                                                                               <  1:241182/150‑1 (MQ=255)                    aTTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTag                                                                                                                                <  1:88402/149‑1 (MQ=255)                     tttACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGt                                                                                                                             >  1:340519/1‑151 (MQ=255)                          gACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACt                                                                                                                        <  2:546268/151‑1 (MQ=255)                               ccGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTc                                                                                                                    >  2:23663/1‑150 (MQ=255)                                              gCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGa                                                                                                    <  2:617812/151‑1 (MQ=255)                                                 gTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAgg                                                                                                 <  1:294580/151‑1 (MQ=255)                                                       aCCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGcc                                                                                           <  2:340112/151‑1 (MQ=255)                                                        cccGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCg                                                                                          >  1:13106/1‑151 (MQ=255)                                                              gATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAAttt                                                                                    >  2:309103/1‑151 (MQ=255)                                                               aTTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTc                                                                                   <  1:464681/151‑1 (MQ=255)                                                                    tGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTg                                                                              >  1:643204/1‑151 (MQ=255)                                                                           gcACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGa                                                                                                        <  1:156428/118‑1 (MQ=255)                                                                           gcACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGa                                                                                                        >  2:156431/1‑118 (MQ=255)                                                                           gcACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACa                                                                       <  2:567190/151‑1 (MQ=255)                                                                                 tATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGc                                                                  >  2:113773/1‑150 (MQ=255)                                                                                         cGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTc                                                         <  2:136977/151‑1 (MQ=255)                                                                                           attattCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCa                                                        >  1:425179/1‑150 (MQ=255)                                                                                                   tGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTg                                               >  1:472435/1‑151 (MQ=255)                                                                                                    gggTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCAtt                                                <  2:509348/149‑1 (MQ=255)                                                                                                       tGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCAcc                                           >  2:104451/1‑151 (MQ=255)                                                                                                           ggTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTa                                       <  2:100569/151‑1 (MQ=255)                                                                                                              cgatATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACAGTTAAcc                                    >  1:115274/4‑151 (MQ=255)                                                                                                               gCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCg                                   >  1:441168/1‑151 (MQ=255)                                                                                                                         cgcgcgCCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTg                          >  1:345241/1‑150 (MQ=255)                                                                                                                           cgcgcCCGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGt                       <  2:153444/151‑1 (MQ=255)                                                                                                                                 cGTTTTCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCt                  >  1:593597/1‑150 (MQ=255)                                                                                                                                      tCATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAAccc            >  2:172067/1‑151 (MQ=255)                                                                                                                                       cATGATGGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAAccc            <  1:523880/150‑1 (MQ=255)                                                                                                                                        atgatgGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCgg          >  1:155391/1‑151 (MQ=255)                                                                                                                                          gatgGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGc         <  1:113770/150‑1 (MQ=255)                                                                                                                                            tgGTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCaa       >  1:110696/1‑150 (MQ=255)                                                                                                                                             ggTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCaaa      <  2:433375/150‑1 (MQ=255)                                                                                                                                             ggTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCAaag     <  1:357720/151‑1 (MQ=255)                                                                                                                                              gTTCGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCaa       >  2:237376/1‑148 (MQ=255)                                                                                                                                                 cGCTCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCAaagaag  <  1:219285/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGCAGCTGTACGACACGCTATTTACGACTTCCGATGACGAACCAGGCAGTTATTACCCGCTGATTGCTGAAAGCGCACGCTATGCTGACGATTATTCCTGGGTGGAGGTCGCTATTAATCCGCGCGCCCGTTTTCATGATGGTTCGCCCATTACTGCCCGCGATGTAGAGTTTACTTTTCAAAAATTTATGACCGAAGGCGTGCCGCAATTTCGTCTGGTCTACAAAGGCACCACCGTCAAAGCCATTGCACCGTTAACCGTGCGCATTGAGTTAGCTAAACCCGGCAAAGAAG  >  NC_000913/2272605‑2272898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑