Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,460,130	T→C	M114V (ATG→GTG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,460,130	0	T	C	100.0%	122.0 / NA	34	M114V (ATG→GTG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/20);  total (14/20)

GCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCATCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAC  >  NC_000913/2459984‑2460268                                                                                                                                                   |                                                                                                                                           gCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGTCACCAgg                                                                                                                                        <  2:481831/151‑1 (MQ=255)   gagagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCt                                                                                                                                      >  1:328532/1‑151 (MQ=255)     gagaAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCttt                                                                                                                                    <  1:596218/151‑1 (MQ=255)            gTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGAc                                                                                                                             >  2:390231/1‑151 (MQ=255)               tCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGtt                                                                                                                          >  1:432914/1‑151 (MQ=255)                            cATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTg                                                                                                              <  1:364882/150‑1 (MQ=255)                             aTGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTgg                                                                                                             >  2:555535/1‑150 (MQ=255)                                   cGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTgg                                                                                                      >  2:64416/1‑151 (MQ=255)                                         ttgttgACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAgc                                                                                                  <  1:230558/149‑1 (MQ=255)                                                   cAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCt                                                                                      <  1:205143/151‑1 (MQ=255)                                                        aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  1:23738/1‑151 (MQ=255)                                                        aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  2:295424/1‑151 (MQ=255)                                                        aGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGc                                                                                 >  2:224839/1‑151 (MQ=255)                                                                   cAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCg                                                                      <  1:64416/151‑1 (MQ=255)                                                                      ttttttACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGt                                                                    >  1:407296/1‑150 (MQ=255)                                                                        ttttACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTAt                                                                  <  1:495262/150‑1 (MQ=255)                                                                           tACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTa                                                               <  2:592720/150‑1 (MQ=255)                                                                                  gCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCATGCTTTCTGCGACGTTTGCAACTGCCTGGTAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTAcattcatt                                                       <  2:215768/151‑1 (MQ=255)                                                                                   ccAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACattcattc                                                      <  2:69156/151‑1 (MQ=255)                                                                                     aaTTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTcc                                                     <  1:318981/150‑1 (MQ=255)                                                                                         ggCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTa                                                 <  2:433448/150‑1 (MQ=255)                                                                                            aaTACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCg                                              >  1:178339/1‑150 (MQ=255)                                                                                               aCCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCgaga                                           >  1:391521/1‑150 (MQ=255)                                                                                                      agGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTc                                   <  2:481578/151‑1 (MQ=255)                                                                                                        gAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCa                                  <  1:84454/150‑1 (MQ=255)                                                                                                         aaTCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCAcg                                <  1:44875/151‑1 (MQ=255)                                                                                                                 ccACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCa                            >  2:1042/1‑147 (MQ=255)                                                                                                                 ccACCGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCa                            <  1:1042/147‑1 (MQ=255)                                                                                                                    ccGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTgg                     <  2:403058/151‑1 (MQ=255)                                                                                                                    ccGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTg                      <  1:554679/150‑1 (MQ=255)                                                                                                                     cGGCAATCCCCGCTCGAATAGTTCCCGCCACCAGGATTTCTGCGACGTTTGCAACTGCCTTGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGtttg                     >  1:199521/1‑148 (MQ=255)                                                                                                                            ccccGCTCGAATAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCa             <  2:560527/151‑1 (MQ=255)                                                                                                                                      aTAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTa   <  1:594534/151‑1 (MQ=255)                                                                                                                                       tAGTTCCCGCCACCAGGCTTTCTGCGACGTTTGCAACTGCCTCGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAc  >  1:333093/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                           GCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTTGACATCAACCAGCACGCGCGCCAGTTTTTTACTGACGCCAATTGGCAATACCGAAGAGGAATCTGCCCCACCGGCAATCCCCGCTCGAATAGTTCCCGCCATCAGGCTTTCTGCGACGTTTGCAACTGCCTGGAAACTGGTAGCGCAAGCGCGGCTGACGCTGTAAGCATCGGTATGTACATTCATTCCCGTACCGAGAACAATTTCACGCGCAATGTTGGGGGCTTCAGGCATTTGTAC  >  NC_000913/2459984‑2460268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑