Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,119	T→C	F272L (TTT→CTT)	fadL →	long‑chain fatty acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,119	0	T	C	100.0%	176.3 / NA	48	F272L (TTT→CTT)	fadL	long‑chain fatty acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/21);  total (27/21)

CGCTCATCTGAACGGTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGTTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGG  >  NC_000913/2461971‑2462260                                                                                                                                                     |                                                                                                                                              cGCTCATCTGAACGGTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGctt                                                                                                                                             <  2:508960/151‑1 (MQ=255)          gAACGGTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTa                                                                                                                                     <  2:562373/150‑1 (MQ=255)             cGGTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGt                                                                                                                                 <  2:422449/151‑1 (MQ=255)              ggTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGtt                                                                                                                                <  2:660112/151‑1 (MQ=255)                 aaCCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGc                                                                                                                             <  2:47592/151‑1 (MQ=255)                      gTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGtt                                                                                                                                >  2:45585/1‑143 (MQ=255)                      gTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGtt                                                                                                                                <  1:45586/143‑1 (MQ=255)                       tGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAAtt                                                                                                                        <  1:577673/150‑1 (MQ=255)                              ttGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTAcc                                                                                                                  <  2:285472/149‑1 (MQ=255)                               tGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACcgcg                                                                                                               >  1:171897/1‑151 (MQ=255)                                 gCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGAc                                                                                                             <  1:257096/151‑1 (MQ=255)                                 gCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGAc                                                                                                             >  2:612512/1‑151 (MQ=255)                                            ggAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGcgca                                                                                                   >  1:590933/1‑150 (MQ=255)                                               aTCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGcgcaacgc                                                                                               >  1:249618/1‑151 (MQ=255)                                               aTCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGcgcaacgc                                                                                               >  2:221189/1‑151 (MQ=255)                                                  cTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGcgcaacgca                                                                                              >  1:177409/1‑149 (MQ=255)                                                     tATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCgg                                                                                         >  2:557341/1‑151 (MQ=255)                                                             ggATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCt                                                                                  <  1:612501/150‑1 (MQ=255)                                                               aTAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGAc                                                                               <  1:316699/151‑1 (MQ=255)                                                                   aaaTAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTg                                                                           >  2:522000/1‑151 (MQ=255)                                                                    aaTAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGa                                                                          <  2:119979/151‑1 (MQ=255)                                                                         ccGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAAcctg                                                                     >  2:33019/1‑151 (MQ=255)                                                                                 cACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAAt                                                             <  1:344144/151‑1 (MQ=255)                                                                                 cACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAAt                                                             >  2:383385/1‑151 (MQ=255)                                                                                  aCTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAAtg                                                            <  2:590938/151‑1 (MQ=255)                                                                                     gACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAAtgt                                                           <  2:531856/149‑1 (MQ=255)                                                                                     gACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTgg                                                         >  2:616568/1‑151 (MQ=255)                                                                                     gACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTgg                                                         <  1:380790/151‑1 (MQ=255)                                                                                       ccTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTggg                                                        >  1:421988/1‑150 (MQ=255)                                                                                            cGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAgtgt                                                  >  2:257611/1‑151 (MQ=255)                                                                                             gTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTc                                                 >  2:58828/1‑151 (MQ=255)                                                                                                  gAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGtt                                            >  1:247005/1‑151 (MQ=255)                                                                                                   aaGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTta                                           <  1:537577/151‑1 (MQ=255)                                                                                                    aGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTtat                                          >  2:211652/1‑151 (MQ=255)                                                                                                    aGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTtat                                          <  1:471285/151‑1 (MQ=255)                                                                                                       gAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAAc                                       >  2:247853/1‑151 (MQ=255)                                                                                                         aaaTTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCg                                     <  2:504051/151‑1 (MQ=255)                                                                                                         aaaTTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCg                                     >  1:600671/1‑151 (MQ=255)                                                                                                           aTTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCgtg                                   >  2:311110/1‑151 (MQ=255)                                                                                                              gACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGtt                                 <  2:603202/150‑1 (MQ=255)                                                                                                                  tCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATcc                            >  2:74852/1‑151 (MQ=255)                                                                                                                    aaaGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCcac                          >  2:295760/1‑151 (MQ=255)                                                                                                                    aaaGGTAACTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCcac                          >  1:83351/1‑151 (MQ=255)                                                                                                                          aaCTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTgg                     <  1:211651/150‑1 (MQ=255)                                                                                                                            cTACAGCAGCGATCTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCg                  >  1:256913/1‑151 (MQ=255)                                                                                                                                         cTTAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGcc     >  2:106136/1‑151 (MQ=255)                                                                                                                                           tAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCt    <  1:552513/150‑1 (MQ=255)                                                                                                                                           tAATCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTg   >  1:17794/1‑151 (MQ=255)                                                                                                                                            aaTCGTGCGCTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTgg  >  1:351536/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                              CGCTCATCTGAACGGTAACCAGTGGGGCTTTGGCTGGAACGCCGGAATCCTGTATGAACTGGATAAAAATAACCGCTATGCACTGACCTACCGTTCTGAAGTGAAAATTGACTTCAAAGGTAACTACAGCAGCGATCTTAATCGTGCGTTTAATAACTACGGTTTGCCAATTCCTACCGCGACAGGTGGCGCAACGCAATCGGGTTATCTGACGCTGAACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGG  >  NC_000913/2461971‑2462260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑