Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,530,256	G→A	A326A (GCC→GCT)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,256	0	G	A	100.0%	104.2 / NA	27	A326A (GCC→GCT)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

GATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCT  >  NC_000913/2530110‑2530401                                                                                                                                                   |                                                                                                                                                  gATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCg                                                                                                                                                <  2:494570/150‑1 (MQ=255)       gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGa                                                                                                                                         <  1:400056/151‑1 (MQ=255)          atTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACtt                                                                                                                                      >  1:259183/1‑151 (MQ=255)           ttCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTc                                                                                                                                     >  2:641682/1‑151 (MQ=255)            tCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTcg                                                                                                                                    >  2:398953/1‑151 (MQ=255)                    gCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGgatgat                                                                                                                            <  2:321236/151‑1 (MQ=255)                    gCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGgatgat                                                                                                                            <  2:248557/151‑1 (MQ=255)                     cGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGatgatg                                                                                                                           >  1:538947/1‑151 (MQ=255)                      gAAGCGTCGTTCGCAGTTCCGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGt                                                                                                                          >  2:199618/1‑151 (MQ=255)                          cgtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                                                                      >  1:642719/1‑151 (MQ=255)                          cgtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                                                                      <  2:133251/151‑1 (MQ=255)                              gtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTAc                                                                                                                  >  1:548255/1‑151 (MQ=255)                                                     ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGtcat                                                                                           >  2:231118/1‑151 (MQ=255)                                                       cGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGtcatca                                                                                         <  1:60123/151‑1 (MQ=255)                                                        gattgattCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTcatcat                                                                                        <  1:577976/151‑1 (MQ=255)                                                                              cAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCAc                                                                   <  2:590207/150‑1 (MQ=255)                                                                                     aaaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGc                                                           >  2:235333/1‑151 (MQ=255)                                                                                      aaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGcc                                                          >  2:346778/1‑151 (MQ=255)                                                                                      aaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGcc                                                          >  1:461308/1‑151 (MQ=255)                                                                                                          gTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATAtgct                                      <  2:121586/151‑1 (MQ=255)                                                                                                              gAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTgcgc                                  >  2:135163/1‑151 (MQ=255)                                                                                                               aaGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTgcgc                                  >  1:157508/1‑150 (MQ=255)                                                                                                                    gagTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTg                            >  2:56581/1‑151 (MQ=255)                                                                                                                                  tAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTg               <  2:460589/150‑1 (MQ=255)                                                                                                                                         aTCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGTGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCt        <  1:49448/150‑1 (MQ=255)                                                                                                                                            aGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGc      >  2:261452/1‑149 (MQ=255)                                                                                                                                              gCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCt  <  2:476005/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCT  >  NC_000913/2530110‑2530401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑