Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,684,690 G→A A273V (GCG→GTG)  glyA ← serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,684,6900GA97.4% 130.4 / NA 38A273V (GCG→GTG) glyAserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (24/13);  total (25/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGG  >  NC_000913/2684548‑2684834
                                                                                                                                              |                                                                                                                                                
cagGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCg                                                                                                                                          >  1:472722/1‑151 (MQ=255)
             ttATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGc                                                                                                                              >  1:285010/1‑150 (MQ=255)
              tATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCAt                                                                                                                            >  2:574578/1‑151 (MQ=255)
                       cgccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGAcc                                                                                                                   >  1:207091/1‑151 (MQ=255)
                        gccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCg                                                                                                                  <  2:508463/151‑1 (MQ=255)
                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGAt                                                                                                                                     <  2:375929/128‑1 (MQ=255)
                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGAt                                                                                                                                     >  1:375925/1‑128 (MQ=255)
                            gAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCt                                                                                                              <  2:605405/151‑1 (MQ=255)
                                     ttGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGAccacca                                                                                                      <  1:562483/150‑1 (MQ=255)
                                             gcgcTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACg                                                                                             >  1:226022/1‑151 (MQ=255)
                                                cTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGc                                                                                           >  2:288988/1‑150 (MQ=255)
                                                cTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGc                                                                                           >  1:481391/1‑150 (MQ=255)
                                                   gAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCag                                                                                         >  1:108317/1‑149 (MQ=255)
                                                     ggAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGtt                                                                                     >  1:352921/1‑151 (MQ=255)
                                                       aaCACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCa                                                                                   <  1:34794/151‑1 (MQ=255)
                                                           cTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGCAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGttt                                                                               >  1:178364/1‑151 (MQ=255)
                                                            ttCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGtttt                                                                              >  2:224765/1‑151 (MQ=255)
                                                                   aTCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTAc                                                                        >  1:351632/1‑150 (MQ=255)
                                                                     cGCTTTAGCGTTTCTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGc                                                                     <  2:529914/151‑1 (MQ=255)
                                                                          tAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCtt                                                                 <  2:317431/150‑1 (MQ=255)
                                                                                   tAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTacca                                                         >  1:643845/1‑149 (MQ=255)
                                                                                    aGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTaccacc                                                       <  2:285009/150‑1 (MQ=255)
                                                                                       gACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTc                                                   >  1:82424/1‑151 (MQ=255)
                                                                                         ccTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGc                                                 <  1:144365/151‑1 (MQ=255)
                                                                                          ctgctgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGcc                                                >  1:474497/1‑151 (MQ=255)
                                                                                              tgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAgg                                             >  1:548503/1‑150 (MQ=255)
                                                                                              tgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAgg                                             >  2:489787/1‑150 (MQ=255)
                                                                                                  ggTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATc                                          >  2:531417/1‑149 (MQ=255)
                                                                                                   gTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAgg                                       <  2:352924/151‑1 (MQ=255)
                                                                                                     gagttgggAACTCAGGCTCCAGGGCAACGTGCAGAGCAAACGCTTTACCGGCGCTGGCGTGCGTCAACGGACAAAAAAAACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGgcc                                     >  1:53897/8‑151 (MQ=255)
                                                                                                              aCTCAGGCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGGTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCgg                            >  2:252701/1‑151 (MQ=255)
                                                                                                                    gCTCCATCGCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGc                      >  1:611927/1‑151 (MQ=255)
                                                                                                                            gCTTCTTTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGttt              >  2:122551/1‑151 (MQ=255)
                                                                                                                              ttcttTCAGAGCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGtttt             >  2:448891/1‑150 (MQ=255)
                                                                                                                                     agagCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAgtgg     >  1:346164/1‑151 (MQ=255)
                                                                                                                                     agagCAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAgtg      <  2:589875/150‑1 (MQ=255)
                                                                                                                                         cAACCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGtggtgg  <  1:505795/150‑1 (MQ=255)
                                                                                                                                          aaCCACTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGCCCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGtggtgg  <  1:574573/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGG  >  NC_000913/2684548‑2684834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑