Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,706	C→A	intergenic (‑156/‑53)	pgpC ← / → yfhH	phosphatidylglycerophosphatase C, membrane bound/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,706	0	C	A	100.0%	157.1 / NA	43	intergenic (‑156/‑53)	pgpC/yfhH	phosphatidylglycerophosphatase C, membrane bound/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/18);  total (25/18)

CTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGC  >  NC_000913/2698567‑2698853                                                                                                                                            |                                                                                                                                                    cTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGtctc                                                                                                                                          >  1:141905/1‑151 (MQ=255)       cATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTg                                                                                                                                    >  1:198307/1‑151 (MQ=255)          ccTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCt                                                                                                                                 >  1:660009/1‑151 (MQ=255)             gATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCtgtt                                                                                                                              >  2:336900/1‑151 (MQ=255)                 aaTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTc                                                                                                                           <  2:204881/150‑1 (MQ=255)                 aaTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCg                                                                                                                          >  1:58953/1‑151 (MQ=255)                  aTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGc                                                                                                                         <  2:51302/151‑1 (MQ=255)                  aTTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGc                                                                                                                         >  2:392526/1‑151 (MQ=255)                    tttCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGcc                                                                                                                        <  2:323456/150‑1 (MQ=255)                             tacATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCAt                                                                                                              >  2:69056/1‑151 (MQ=255)                                      tAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGa                                                                                                      >  1:358585/1‑150 (MQ=255)                                       aaCAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGaaa                                                                                                    <  2:111808/151‑1 (MQ=255)                                              agatCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATg                                                                                              <  1:450091/150‑1 (MQ=255)                                                     caCTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCtt                                                                                      <  2:198308/151‑1 (MQ=255)                                                          ttCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACtt                                                                                  <  1:651703/150‑1 (MQ=255)                                                                caacaaTCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATcc                                                                           <  1:77278/151‑1 (MQ=255)                                                                       cGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCg                                                                    >  2:126040/1‑151 (MQ=255)                                                                               tcatGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAgg                                                            >  2:62816/1‑151 (MQ=255)                                                                                    gCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCtt                                                       <  1:576793/151‑1 (MQ=255)                                                                                      tAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGc                                                     >  1:501856/1‑151 (MQ=255)                                                                                        aCTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   <  2:488861/151‑1 (MQ=255)                                                                                         cTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   >  1:238074/1‑150 (MQ=255)                                                                                           ggAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGccc                                                   >  2:45909/1‑148 (MQ=255)                                                                                           ggAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCaga                                                <  1:251438/151‑1 (MQ=255)                                                                                            gAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATACTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCag                                                 >  1:111886/1‑149 (MQ=255)                                                                                               tttttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTg                                             >  2:426345/1‑150 (MQ=255)                                                                                                 tttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGAt                                           >  2:151377/1‑150 (MQ=255)                                                                                                  ttAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATa                                          >  1:503/1‑150 (MQ=255)                                                                                                   tAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATaa                                         <  2:548188/150‑1 (MQ=255)                                                                                                   tAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATaa                                         <  1:217124/150‑1 (MQ=255)                                                                                                          tGGCAATTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTgg                                 >  1:45273/1‑151 (MQ=255)                                                                                                               aTTAGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGAt                            >  1:51257/1‑151 (MQ=255)                                                                                                                  aGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACTAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTa                          <  1:573438/150‑1 (MQ=255)                                                                                                                  aGCGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTa                          <  1:17384/150‑1 (MQ=255)                                                                                                                    cGGCAATGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATct                       <  1:150432/151‑1 (MQ=255)                                                                                                                        aaTGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATctgctg                   >  1:293938/1‑151 (MQ=255)                                                                                                                          tGGAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTgctgct                 >  1:551255/1‑151 (MQ=255)                                                                                                                           ggAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTCACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTgctgct                 >  1:493556/1‑150 (MQ=255)                                                                                                                            gAATATAAAATTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTAc               >  2:156735/1‑151 (MQ=255)                                                                                                                                     aTTCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATa      <  1:372420/151‑1 (MQ=255)                                                                                                                                      ttCACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCGGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATAc     >  1:379763/1‑151 (MQ=255)                                                                                                                                        cACTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACgg   >  1:479577/1‑151 (MQ=255)                                                                                                                                         aCTAGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGgc  <  1:284376/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                    CTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGC  >  NC_000913/2698567‑2698853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑